Detecting gene mutations: ASO technique

www.sicklecella...
This video gives an overview aimed at science students to explain a common laboratory technique for detecting genetic mutations. In this video, sickle cell disease is used as a case in point. ASO stands for 'allele-specific oligonucleotide' hybridisation. A short piece of DNA is synthesised to act like a probe that can then detect certain mutations in patient samples.
This is an open educational resource part of the 'SCOOTER Project' funded in the UK by the Jisc/HEA Open Educational Resource Programme. We aim to support the teaching of sickle cell and raise awareness of the condition.

[@pranitdhabarde6845]

Being an engineering student, I was able to understand most of the content here. Amazing video. Helped a lot.

[@shivavarma7937]

Thankyou Ma'am, Can you please clarify why the probe used is same as the sequence and not complementary to the gene.

[@saadiamohammed6737]

Thank you! Helped so much :)

[@EilafBadr--]

Thank you a lot