Allele Specific PCR | ARMS PCR |

A method which is used to detect a single base change or we can SNP, using sequence-specific primers.
The techniquie has other names like
ARMS... Amplification Refractory Mutation System.
PASA .... PCR Amplification of Specific Allele
AS- PCR ...for Allele-Specific PCR.
In all these acronyms, everytime we are using the word “Allele”...
the reason being is its specific amplification capacity for a specific allele or DNA sequence.
So,
What is ARMS or Allele-Specific PCR?
As we know Alleles are alternative forms of a gene.
When a mutation occurs, it give rise to, two different alleles for a gene;
one type is wild or normal and onother one is mutant.
The Allele-specific PCR has the ability to detect this specific allele.

[@juandavidsaavedra6525]

Excellent

[@francescabottona783]

Helpful !! Thank you!

[@povilaskasauskas1760]

A very nice explanation of the mutation tracing technique!

[@BMHlearning]

Glad it was helpful!

[@RanaAhmed-ch1vz]

Good 👍

[@gvg9586]

thank you. Could you tell me wwhich one is the constant primer and which one is the standard primer?

[@minimoni3568]

Sir please help me understanding difference between tetra arms pcr and arms pcr only.

[@MohammadMahmoud-ji1jc]

I have created an arms primer; might I perhaps email it for revision?

[@ghaida3860]

Is this method used to detect one SNP at a time ?

[@BMHlearning]

Yeah

[@Marshhc910]

do we proceed with electrophoresis next?

[@BMHlearning]

Yeah