Hybridization (microarray) | Biomolecules | MCAT | Khan Academy

Visit us (www.khanacademy...) for health and medicine content or (www.khanacademy...) for MCAT related content. These videos do not provide medical advice and are for informational purposes only. The videos are not intended to be a substitute for professional medical advice, diagnosis or treatment. Always seek the advice of a qualified health provider with any questions you may have regarding a medical condition. Never disregard professional medical advice or delay in seeking it because of something you have read or seen in any Khan Academy video. Created by Ronald Sahyouni.
Watch the next lesson: www.khanacadem...
Missed the previous lesson? www.khanacadem...
MCAT on Khan Academy: Go ahead and practice some passage-based questions!
About Khan Academy: Khan Academy offers practice exercises, instructional videos, and a personalized learning dashboard that empower learners to study at their own pace in and outside of the classroom. We tackle math, science, computer programming, history, art history, economics, and more. Our math missions guide learners from kindergarten to calculus using state-of-the-art, adaptive technology that identifies strengths and learning gaps. We've also partnered with institutions like NASA, The Museum of Modern Art, The California Academy of Sciences, and MIT to offer specialized content.
For free. For everyone. Forever. #YouCanLearnAnything
Subscribe to Khan Academy’s MCAT channel: / @khanacademymcatprep
Subscribe to Khan Academy: www.youtube.co...

[@g2k25d94]

I can't express how much this made sense at such a crucial time. Thanks a bunch.

[@chandrashekharchauhan4120]

Just wow.... How do you guys at khan academy make such brilliant content?? Such complicated stuff explained with such clarity and made really simple, as if I was watching it happen, live in the lab.
Thanks and love from India

[@brookecarlson9110]

what the heck is wrong with the audio? It's repeating itself, and then sometimes disappearing..

[@danielmillan6144]

Don't listen what the people say thanks man you make amazing videos

[@Misstigrine]

cDNA from the mRNA are used. In the wells, there are single strand DNA not RNA.

[@darrelltan7603]

thanks bro

[@shaki6500]

some of these videos are so wrong and confusing :( thank you i was like what happen to cDNA part of it

[@sabil1998]

right that makes more sense

[@rasheedolajiire5135]

Thanks a gazillion for the clarification!

[@ruhidyusifov5106]

Thank you for helping by sharing you guys are brilliant.

[@1992deval]

This video has made understanding so simple, and its 7 years old yet the best video

[@juliedao6860]

Thanks a lot! Very thorough and clear

[@TheOnlyRero]

i love khan academy videos .. but some of them like this one has a very low audio i can barley hear it!

[@nu.wa.n]

+TheOnlyRero If you are using Windows, go to your speaker properties and make sure 'Loudness equalization' is ticked under the Enhancements tab.

[@britneymichelle4]

THANK YOU!! I can finally hear this!!

[@sylee9165]

@@nu.wa.n Thanks!!

[@lolacasielleslapeira8936]

i love you guys, this is just fantastic!

[@manlikanamkaew2220]

Thank you~

[@arpittyagi6404]

Thanks..helps a lot..

[@JimRohn-u8c]

Thank You so much! God Bless!

[@DrFelix125]

His mic is bit on the quiet side, but I've heard worse. Good explanation, thx!

[@sciencenerd7639]

amazing!

[@zhansayabauyrzhanova2492]

here is my question: how to label with yellow and blue? could u explain the mechanism of it? thank you for considering!

[@Nisia44]

omg it was sooo helpfull, thank you VERY much ;)

[@vuongyenxuan]

Thank you so much.

[@s.b.5896]

Thank you very much :)

[@FabriceTheArtist]

Great video. But, in my textbook it says that the wells are filled with DNA, not mRNA.

[@kaustek]

There are different kinds of microarrays -- gene microarrays (DNA) and gene-expression microarrays (mRNA).

[@ZAsym]

@@kaustek ahh makes sense

[@merna2724]

THANK YOU SOOOOOOO MUCH

[@Colderlarry1]

GRACIAS

[@uroojaarif9943]

I have a confusion about the radio labeling (yellow dots & blue dots) how is it done ? How can one know whether one is a cancer cell or normal cell in the first place ?

[@cdorman11]

As to the second question, it's the same as knowing where to take the biopsy from. It's what's growing rapidly, and showing up as dense tissue in an X-ray.

[@AnkushSharma-zv5hv]

good

[@ErinRaciell]

Jesus I’m barely awake for my biotech midterm and this guys enthusiasm it’s not helping...

[@ss-nu8xx]

Yeah he is not your keeper. His job isn’t to keep you awake. Wtf

[@2legit2hit]

Cuts out at:
0:25
1:35

[@jsantana331]

In the well, would their be complementary RNA, or ssDNA complementary to the probed cDNA ?

[@mikell.6064]

The video was very poorly explained. The well doesn't contain RNA. It contains ssDna that hybridizes with complementary DNA from sample. The sample is obtained from reverse transcribing the RNA from the cell to cDNA. This CDNa is used to hybridizes with the well.
So in short the answer to your question is ssDNA

[@sarahali1788]

I swear his voice is Armando hasudungan ! Biology and medicine you tuber?

[@atti1120]

so is each well complementary to only 1 gene each?

[@عمارالاسمري-ت4ف]

Please try to raise your voice, Couldn't complete the list because of that!

[@juzy1022]

😃😃

[@sundasrashid6026]

dude just go to sleep

[@isaackogan269]

its 2:24am lmfaoooo hlep me

[@sukhpreetsingh5603]

plz get some sleep

[@sara-qz8zw]

Is there anyone here to help me? . My master asked us to find (the mRNA -DNA ybridization use for which microbes?) Pleaseeee help me

[@kamilab131]

does up regulation mean the same as duplication of a specific gene ?

[@sumitgupta7983]

Upregulation means amplification of gene

[@Vencomycin]

Where is cDNA???

[@Bar0n0s]

we need some good subs. good work though

[@rimaakter2116]

Low sound

[@arrankambo3665]

this video needs a far better mic. i can barely hear what he's saying

[@soumasoum2048]

I need explication in French

[@Asura6969]

non

[@menaameer6848]

sorry but you didn't make me understand

[@mohammado.eshkalak3140]

This guy has no enthusiasm whatsoever to teach. He must make you wake up instead of making you sleep. Dont like it.

[@christiankorkis630]

Kinda sounds like this guy needs a nap like Bernie

[@rizaln8315]

boring

[@EilafBadr--]

Thanks a lot .This is so helpful

[@gustavojuantorena]

Thank you!

[@dreamerscastle]

Thank you very much !