G : dimethyl sulfate ft hot piperidine (90°C) C : hydrazine w 1.5M NaCl hot piperidine (90°C) C & T : hydrazine w/o NaCl hot piperidine (90°C) G & A : formic acid hot piperidine (90°C) 1. Cut fragments 2. Separate using PAGE 3. Autoradiography 4. Data Analysis (bottom to top)
thanx, this is really helpful but I have a question that I can't find the answer for: What do we have todo to label only one of the 2 strands of the molecule once we denaturate it?
You're so copying the style of that Guy from AK lectures lol. Please, go with a straight Indian accent, it's sort of annoying. Very useful tutorial nevertheless!
G : dimethyl sulfate ft hot piperidine (90°C)
C : hydrazine w 1.5M NaCl hot piperidine (90°C)
C & T : hydrazine w/o NaCl hot piperidine (90°C)
G & A : formic acid hot piperidine (90°C)
1. Cut fragments
2. Separate using PAGE
3. Autoradiography
4. Data Analysis (bottom to top)
DUDE. I LOVE YOU. THANK YOU VERY MUCH FOR THIS
Sincerely,
a struggling molecular bio student
molecular bio... have you completed ur degree?
Thanks after searching for few minutes to explain how sequence is made by seeing gel,I got
Thanks 🎉
Thank you . I have an exam tomorrow and you made it a lot easier🎉
Me too 😂
Any one from bsc biotech???
🙋♀️
Yes
Me
Doing msc in that bro 🙃
@@highlightspro8274 from which university
I am very greatful for this channel
thx for the explanation. it helps me a lot
Thanks so much+! This was much more concise than every other video on this and far more clear!
Thank you this vedio cleared all textbook points
You just saved me ❤Thiz help me to take seminar 🤧💞
Thank you so much❤️
AMAZING EXPLANATION!
Iam in B.sc.How the heck do they expect us to understand without practical( cus of corona) thanks bro for your help
Amazing explanation..
Keep doing good work❤
Best sequencing video
Thank you so much a clear cut explanation ❤️
Nice video
Wonderfully explained 😌
Thanks for the video !
Thank you.⭐
Thank you for this amazing explanation!!! I finally understand this!
Awesome video
Thank you so much for the video! It's really helpful
great video, really hepled!
Thank you very much ❤❤
Thank you so much.. Its so helpful..
you compare lines based on what ?
thanks so much❤❤❤
Thank you so much!
thanx, this is really helpful but I have a question that I can't find the answer for: What do we have todo to label only one of the 2 strands of the molecule once we denaturate it?
could you please make videos for all the NGS methods? O:)
Are base specific cleavage reactions done on single stranded DNA?
Very well explained.. Thankyou so much
. pls make more vedios
You are the besttttttttt
Thanks. Quite simple
Thanks a lot
Bosssss thank You ❤️
Good teacher
i have a question
I Love this video
thanks a lot. God bless
Thanks for this atleast i get it now
THANK U
Thanks
Outstanding
Love it thankyou
Yes
I am
Great
Great..
Best
❤️❤️❤️❤️❤️
👍
❤
You're so copying the style of that Guy from AK lectures lol. Please, go with a straight Indian accent, it's sort of annoying. Very useful tutorial nevertheless!
Thanks so much+! This was much more concise than every other video on this and far more clear!
Thanks