Pyrosequencing

This video is about pyrosequencing method of DNA sequencing. Pyrosequencing is a sequencing technique in which, the incorporation of dNTP in the DNA is detected in The form of light. If dNTP is not incorporatred, no light is detected. hence the sequence information of the DNA can be easily obtained by detecting the light.

[@kristenapostol6288]

OHMYGOSSH, this is PERFECT! Exactly what I needed for my exam tomorrow, and explained the purpose behind everything, too! Thank you so much!!!

[@rossmoorman9335]

Thank you. The PowerPoint the teacher gave for the test is garbage, but this clearly explains the concept.

[@eneaventurini1621]

This video is really clear and precise. Thank you!

[@studybooks3395]

thank youuuu!!!! I never understood how pyrosequencing diferentiate each nucleotide, until this video.

[@user-jp6qx5ky7u]

This video was so helpful for me to understand the principle of pyrosequencing. Thank you so much!

[@giddygiddybangbang9425]

What an amazing video! Thank you for the clear and concise explanation.

[@manospapadopoulos9816]

Such a beautiful video. Made the concept crystal clear🙂

[@noahjendrasheske1843]

Thanks! My professor did not say anything about the flow cell. This clears everything up.

[@martapastori9847]

extremely useful!!! thank you very much :)

[@mawinstallation6626]

Brother your channel is a godsend 🙏

[@mastersacademy5393]

This really amazing and very easy for learning.

[@geovanniruiz359]

Amazing explanation ! Congratulations and thank you!

[@aliceyonemin241]

That's so useful and easy to understand, thank you very much

[@trainingmedic26]

Thank you! This was really helpful.

[@camilocastelblanco2903]

Bro, you make this topic very easy. Thank you

[@priyakamboj5850]

This vedio make my concept clear thanku so much sir

[@Polarcupcheck]

Videos are always easy to understand.

[@piano_master_5246]

they're much easier to understand than text or oral explanations, the Latin terminology on its own stops one from understanding anything lolz

[@helenpessoa8542]

Amazing video. Thank you

[@jozzatheg]

Fantastic vid! Thanks so much for creating this

[@jideofoonwuli5709]

Just made my work easy... Thanks

[@quickbiochemistrybasics]

Glad it helped

[@ramchandrasuthar2848]

Nice analysis

[@Omar-gm2zq]

Awesome video thanks

[@freddykidd6301]

Amazing explanation - thank you! :)

[@malt8596]

Bless you! You've just made my studying so much simpler! Thanks you!! :-D

[@gretamolinaro2567]

Very clear video!!

[@rumad7775]

perfectly explained. thank you so much!

[@sivasankar372]

made easy to understand

[@rav9066]

thanks for teaching me what naomi levi strumpff couldnt

[@kirtiahlawat2181]

Nice explanation

[@irtizakhan7456]

perfect video sir

[@helenateixidor6932]

Great video, thanks for doing it!!!😊😊😊

[@thefayadbrothers1170]

Simple and clear!!

[@riyaroy7386]

Thank you

[@replicationcellulaire9594]

There should be an issue here if I understand well. At 3', during the example, the added nucleotides are in the order : G A T C A G A T etc... However when the second template is "GC", dATP is added, and nothing happens for template 2, only template 1 gets light. But then there is dGTP then an other dATP, WHICH THIS TIME CAUSES LIGHT. Why does the first dATP did not cause light in template 2 ? Just a little error on the video or am I missing something ?
Great explanation otherwise

[@jovanabogdanovic7099]

Thank you very much 💞

[@Frameorderfanreaction2M2.O]

Thanks a lot

[@patriciatak4987]

amazing video

[@FnaffFifii]

How do u know the specific sequence?
The dNTPs can be matching the nucleotides in the template strands randomly..

[@whatman956]

They have to be added in sequence, also, it's the phosphodiester bonding that releases pyrophosphate not hydrogen bonding.

[@user-oi1ds3hk2n]

Thank you sooooooo much !!!

[@ilarialombardi2368]

Thanks

[@rakshitasingh6574]

Thankyou sir

[@ayaqz3144]

thank youuuu

[@marianachartian]

Amazing video and great explanatn too but i juz have a silly doubt...can u plzz clarify..if we add a dNTP to the flow cell and LIGHT gets produced, then doesnt it mean dat the corresponding base in the template is complementary to the dNTP added?? For eg. if we add dGTP and get LIGHT, wont the corresponding base in the template be 'C' den?? The video shows dat it wud be 'G'....so im a bit confused..plz clarify sir..thank u in advance..😇

[@quickbiochemistrybasics]

Thank you very much for your question.
Yes the corresponding base in the template will be "C".
the nucleotide that has been added is "G"
The video shows simplified animation about the technique.

[@marianachartian]

Quick Biochemistry Basics okie....gud to know...thank yew so much for taking the time to clarify...and keep up the great work

[@dagmardepaepe9601]

When you look at the flow gram of a pyrosequence, not all the peaks have the same hight. Why is the light sometimes more intense?

[@paulelling851]

Very helpfull

[@sutapapal8796]

Primer walking DNA sequencing animation not available????

[@anuragsahu1437]

How does the computer know which dNTP is added ? We are performing sequencing because we don't know the sequence. Please answer.

[@mastersacademy5393]

Thank you sir

[@DrGamer-nb7yf]

thx

[@mehandisokatalisumbhaniya7481]

Fabulous 🥰🥰🥰🥰🥰🥰🥰

[@hongleizhao5895]

Fantastic vidio, is it possible to get a copy of the slides?

[@tarteelmohammed3257]

Magic 💕

[@madihakabache9092]

hi qui peut m expliquer en francais comment peut on distinguer entre les nucleotides ajoutees¿

[@nailaimtiaz3617]

Make video for Illuminati and Solid seq

[@alhaeri1]

legend

[@akifgunay6650]

Cool

[@Gardeningismyhobby6667]

Not add only one dNTP ,add only one types of dNTP
Like dATP,dGTP,dTTP & dCTP

[@FarhanAli-qs6dh]

tayyab ,I think you are watching the same video

[@mnacreation5007]

Hi I have a question any one can Help me

[@joshmilne2885]

no

[@lipipragyadevi]

Luciferase 😵‍💫😵‍💫😵‍💫😵‍💫😵‍💫