DNA Microarray (DNA chip) technique

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  • Опубліковано 13 чер 2024
  • Hey Friends,
    DNA Microarrays cover a lot of tasks such as gene expression analysis and genotyping. How this DNA chip technique works, is explained in this animation.
    Best,
    Henrik
    Time Codes:
    0:00 Introduction: Why to use a DNA microarray
    0:33 Sample preparation
    1:15 DNA Microarray chip - Mechanism of Action
    3:01 In the lab
  • Наука та технологія

КОМЕНТАРІ • 105

  • @henrikslab
    @henrikslab  7 місяців тому +2

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  • @ii.noorii
    @ii.noorii 5 днів тому +2

    One of the best videos to explain the DNA microarray, thank you❤❤

  • @jadon5798
    @jadon5798 4 місяці тому +17

    geiler typ man hört den akzent aber du sprichst deutlich und das was du sagst ist sehr verständlich und top erklärt vielen Dank du hast mir den arsch gerettet

  • @monkey6493
    @monkey6493 Рік тому +25

    Better job than my teacher ever could, thanks a lot.

    • @roshan3190
      @roshan3190 11 місяців тому +1

      Exactly 💯😂

  • @simafarokhnejad5882
    @simafarokhnejad5882 5 місяців тому +4

    This video is one of the best explanations of DNA Microarray I've come across on UA-cam. It's concise and efficient, and it is clear even for someone without a background in biology.

  • @V_MA
    @V_MA 2 роки тому +9

    Great! I needed to watch a video about DNA microarray to understand how it works. It helped me a lot! Thank you.

  • @henrikslab
    @henrikslab  Рік тому +7

    From now on you can become a member of this channel! You will have access to some cool emojis like this:.
    This is of course voluntary, but the financial support helps me a lot.

  • @pitkamatka
    @pitkamatka 2 роки тому +11

    Hi Henrik! I just discovered your channel and I think your videos are of great help for me. Thanks for making them :)

  • @Maria-jl5is
    @Maria-jl5is Рік тому +2

    that was brief and so useful, thank you so much

  • @bhavik3201
    @bhavik3201 6 місяців тому +3

    absolutely outstanding explanation. Thank you. Love from india

  • @eloisub902
    @eloisub902 2 роки тому +2

    Really straightforward! TYSM

  • @JulianTheHunter
    @JulianTheHunter 2 роки тому +3

    So simple erklärt, herrlich! Vielen Dank

  • @papridas8979
    @papridas8979 2 роки тому +2

    Thank you🙏 very clear explaination!

  • @sahanahn19
    @sahanahn19 2 роки тому +32

    It would have been very helpful if you could upload the notes of this microarray..
    Explanation is amazing sir!!

  • @yuqingcai3447
    @yuqingcai3447 2 роки тому +2

    This is very helpful! Thank you!

  • @warwar5380
    @warwar5380 8 місяців тому +1

    thank for making complicate topic easier to understand

  • @muffinfighter3680
    @muffinfighter3680 Рік тому +2

    thanks! Now, I understand how this analysis works

  • @ebircsbus6643
    @ebircsbus6643 10 місяців тому +3

    I can hear your german accent a mile away lmao
    This was a amazing explanation bro thx for putting in so much work!

  • @user-ch7xw4ed8u
    @user-ch7xw4ed8u 2 місяці тому

    Amazing! Best one on UA-cam! Thanks

  • @khadeejazardawy971
    @khadeejazardawy971 2 роки тому +2

    thank you so much you made it easy to understand

  • @Mrzaharna
    @Mrzaharna 2 роки тому +2

    Actually it helped me a lot to understand , thanks a lot

  • @m2-instruments
    @m2-instruments Рік тому +2

    Great video, very well explained 👍🏼

  • @callmebonobononim
    @callmebonobononim Рік тому +2

    You are amazing!! Now I can understand finally🎉🎉 thank you soooo much Have a nice day😆😆

  • @sameloliveira6169
    @sameloliveira6169 6 місяців тому +2

    Great explanation, thank you very much

  • @ma.8805
    @ma.8805 2 роки тому +6

    you are the best, thank you

  • @graemelaubach3106
    @graemelaubach3106 2 роки тому +2

    Awesome channel, keep it up!!!

  • @shiweanyswami7371
    @shiweanyswami7371 Рік тому +2

    Thank you it just blessing to me cleared everything thank u so much please upload more vedios am enjoying to study from here and my concepts get more strong cause you made it clear thanks onece again

  • @thefayadbrothers1170
    @thefayadbrothers1170 2 роки тому +2

    Very clear thank you!

  • @tina9753
    @tina9753 11 місяців тому +2

    Very helpful video:) thanks!

  • @halimish
    @halimish 4 місяці тому +1

    that was really helpful thank you⭐️

  • @chickensandos
    @chickensandos Місяць тому +1

    Great video man

  • @narthakiyasendra2917
    @narthakiyasendra2917 2 роки тому +2

    Amazing job

  • @itstimetostudy397
    @itstimetostudy397 Рік тому +1

    Very nice, thanks 👍

  • @melikaabdorrahimi3768
    @melikaabdorrahimi3768 2 роки тому +1

    Amazing!

  • @harekrishnamandal1428
    @harekrishnamandal1428 2 роки тому +1

    Highly conceptional

  • @user-sz5it4wm9f
    @user-sz5it4wm9f 2 роки тому +2

    A good explanation 👍👍

  • @user-yehia
    @user-yehia 8 місяців тому

    Great explanation

  • @bh5169
    @bh5169 2 роки тому +1

    Thank you!

  • @anjalipatel4107
    @anjalipatel4107 5 місяців тому

    Very nice explanation sir😊

  • @AM-hp7ht
    @AM-hp7ht Рік тому +1

    Great , Good job 😍😍😍🤩🥰

  • @dieterandrich1014
    @dieterandrich1014 2 роки тому +1

    very nice very simple

  • @robin2385
    @robin2385 2 роки тому

    well explained video, the best i habe found, but i have a question: so the oligonucleotides must be complementary to the cDNA and the cDNA has the same direcetion as the leading DNA (3' 5'), so the oligonucleotides have the complementary direction of 5' 3'? sorry for the bad english

  • @syedmohammadaanasfarukh890
    @syedmohammadaanasfarukh890 2 роки тому

    Great stuff

  • @mariociencia12
    @mariociencia12 2 роки тому +15

    It is incredible your ability to do concise videos with a lot of information and completely animated! I have just a question: how exactly does the cDNA become fluorescent? All the bases used are fluorescent?

    • @henrikslab
      @henrikslab  2 роки тому +4

      Thanks, that means a lot!
      I did some more in depth research... and there are many ways in practice. One as you mentioned is using fluorescent nucleotides (all dCTPs are labeled). Another option is: using fluorescent forward primers. This paper might help in case you are super interested in the labeling steps:
      www.sciencedirect.com/science/article/abs/pii/S0167701205001806

    • @henrikslab
      @henrikslab  2 роки тому +3

      Another possibility:
      Using 3´end labeling with fluorescent nucleotides + terminal transferase
      (www.future-science.com/doi/10.2144/000112182)

    • @binebum1
      @binebum1 2 роки тому +1

      Super interesting and clear. Wish you could do some videos on the energy metabolism of cancer cells!

    • @henrikslab
      @henrikslab  2 роки тому +3

      @@binebum1 I did one about the warburg effect.. check that out!

  • @SajidAli-zm8fc
    @SajidAli-zm8fc 2 роки тому

    Really nice

  • @tatianaasi3315
    @tatianaasi3315 2 роки тому +2

    Very great explanation, im studying for med school entry

  • @mohammedal-hammadi5085
    @mohammedal-hammadi5085 2 роки тому +1

    Thank you

  • @user-ef3uq9qe9f
    @user-ef3uq9qe9f 6 місяців тому +1

    Nice and easy

  • @prachitrivedi5538
    @prachitrivedi5538 Рік тому +1

    good explanation

  • @afnanashraf5
    @afnanashraf5 6 місяців тому

    Great!

  • @suriyaprakash2252
    @suriyaprakash2252 10 місяців тому +1

    This is very helpful.,! Thank you!
    I want to know about which Bioinformatics tool has been used To (Raw)data analysis.,

  • @panosfragalas3161
    @panosfragalas3161 Рік тому +1

    Thank you alot

  • @sebastianomoscolin267
    @sebastianomoscolin267 7 місяців тому +1

    🔥🔥🔥

  • @doaaismail11
    @doaaismail11 2 роки тому +1

    this is very clear than the dr. explanation

  • @lstrawaya6403
    @lstrawaya6403 2 роки тому +1

    Merci

  • @a_9773
    @a_9773 Рік тому +1

    Kuss geht raus, brudi

  • @maryjakr8538
    @maryjakr8538 4 місяці тому

    Why would the sample 2 material not binding to the array cDNA mean that the gene is not expressed? Could it not just mean, that there is a mutation (e.g. microduplication) that prohibits the probe from binding while the gene can still be translated and expressed into a protein by the cell in a „loss/gain/change of function“-mutation?

  • @bts_rollercosterlifepurplearmy
    @bts_rollercosterlifepurplearmy 6 місяців тому +1

    U save my 7 marks 😀

  • @caasinom8008
    @caasinom8008 2 роки тому +3

    How does hybridization occur? Are the oligonucleotide probes single-strands as is the cDNA or is there a thermocycle similar to PCR?

    • @henrikslab
      @henrikslab  2 роки тому

      Yes, the oligos are single stranded!

  • @ahmetziyaaktas9179
    @ahmetziyaaktas9179 10 місяців тому

    How the cDNAs hybridize with the probes. Probes are one-stranded okay, but aren’t the cDNAs are double-stranded?

  • @sandrawatt
    @sandrawatt Рік тому +2

    Clear and concise explanation! Question: does each sample go on its own microarray, or are they mixed and applied on the same microarray? Thanks!

    • @henrikslab
      @henrikslab  Рік тому +1

      Each sample is typically applied to its own microarray.

    • @henrikslab
      @henrikslab  Рік тому

      However, there is also the possibility to apply multiple samples to a single microarray. This is commonly done with a technique called "multiplexing". It is done by hybridizing different labeled samples to the same microarray, or by using different probes for each sample on the microarray.

    • @sandrawatt
      @sandrawatt Рік тому

      Ah, that's why it was confusing. It seemed like "sometimes". This really clarifies it. Hope it helps others too!

  • @Unreal2424
    @Unreal2424 2 роки тому +2

    Hi, I got few questions
    1 - we agree that we label AFTER retrotranscription during samble preparation?
    2 - The oligonucleotides found in each microarray, are complementary to cDNA ? so they are DNA and not RNA ?

    • @henrikslab
      @henrikslab  2 роки тому +4

      Hi,
      1 - there might be other assays, but mostly you label with fluorescent nucleotides DURING cDNA synthesis
      2 - Yes, they are DNA

    • @Unreal2424
      @Unreal2424 2 роки тому +1

      @@henrikslab thank you !

  • @sketchy1113
    @sketchy1113 2 роки тому +1

    Very easy😁

  • @anmolpardeshi3138
    @anmolpardeshi3138 Рік тому +1

    the health and disease sample complimentary strands that is put in one tube is from the same person or one is from the case and other is from the control?

    • @henrikslab
      @henrikslab  Рік тому

      Technically, that depends on the research question you are asking, both would be possible

  • @urfujji937
    @urfujji937 5 місяців тому

    Thanks for concising it rather than many slides lol.

  • @effiek3886
    @effiek3886 Рік тому +1

    Can we have more samples at the same time;Does this technique only work for cancerous cells or any type of unhealthy cells;

    • @henrikslab
      @henrikslab  Рік тому

      Yes, you can apply multiple samples to a microarray at the same time. This is commonly done using a technique called "multiplexing". Multiplexing allows multiple samples to be processed simultaneously on a single microarray. It is done by hybridizing different labeled samples to the same microarray, or by using different probes for each sample on the microarray.

  • @muhadsaghaffar746
    @muhadsaghaffar746 Рік тому +1

    👍

  • @HenryGhost-dx9gb
    @HenryGhost-dx9gb Рік тому +1

    how do u make ur animation

    • @henrikslab
      @henrikslab  Рік тому

      To 95% I create everything in Powerpoint, only rarely I also use Inkscape

  • @sara-qz8zw
    @sara-qz8zw 7 місяців тому +1

    Is there anyone here to help me? . My master asked us to find (the mRNA -DNA ybridization use for which microbes?) Pleaseeee help me

  • @user-xe4yu2om8j
    @user-xe4yu2om8j Рік тому +1

    Is the oligonucleotide RNA or DNA?

  • @evilrubin2455
    @evilrubin2455 2 роки тому

    Ginge das auch auf Deutsch? :D Nicht sonderlich schwer rauszuhören. Mein ich nit böse, danke für's Video!

  • @PoojaKumari-jl4ly
    @PoojaKumari-jl4ly Рік тому

    From 0:40

  • @oe4022
    @oe4022 2 роки тому

    Thanks for this video ! just a small point here 0:45 "tissue from a healthy cell" doesn't make any sense, you should have said cell from healthy tissue or something like that.

  • @dernachname5240
    @dernachname5240 Рік тому

    das ist ein sehr deutsches Englisch haha.
    ist aber gut erklärt danke :)

  • @robin2385
    @robin2385 2 роки тому +4

    let me guess, you are german, but nice vid

  • @ym.hj_
    @ym.hj_ Рік тому

    Vote park gunwook

  • @hannahx6347
    @hannahx6347 Рік тому +69

    you are German, aren't you?

    • @sameerhussain3709
      @sameerhussain3709 11 місяців тому +3

      He is Indian

    • @ChigaChad
      @ChigaChad 9 місяців тому +4

      Sound indian

    • @goharabrar4191
      @goharabrar4191 7 місяців тому +1

      It's literally in the Chanel description mate🤦🤦

    • @ldngroulette2888
      @ldngroulette2888 6 місяців тому

      Ja er hat einen deutschen Akzent, und jetzt?

    • @adamnogell7663
      @adamnogell7663 18 днів тому +2

      The guys in the replies probably never heard an indian accent lol

  • @mmmmmmmmm4383
    @mmmmmmmmm4383 4 місяці тому

    😢

  • @Destina-du9sl
    @Destina-du9sl Рік тому

    Du bist doch Deutscher, dann füg doch wenigstens Untertitel ein🥲

  • @945395jagan
    @945395jagan Рік тому +1

    How do u apply this technique in microbiology to detect pathogenic microbes?

  • @Mary-eu6bm
    @Mary-eu6bm 2 роки тому +1

    Thank you!

  • @navadeepdorai2918
    @navadeepdorai2918 Рік тому +1

    Thank you