DNA Microarray (DNA chip) technique
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- Опубліковано 13 чер 2024
- Hey Friends,
DNA Microarrays cover a lot of tasks such as gene expression analysis and genotyping. How this DNA chip technique works, is explained in this animation.
Best,
Henrik
Time Codes:
0:00 Introduction: Why to use a DNA microarray
0:33 Sample preparation
1:15 DNA Microarray chip - Mechanism of Action
3:01 In the lab - Наука та технологія
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One of the best videos to explain the DNA microarray, thank you❤❤
geiler typ man hört den akzent aber du sprichst deutlich und das was du sagst ist sehr verständlich und top erklärt vielen Dank du hast mir den arsch gerettet
Better job than my teacher ever could, thanks a lot.
Exactly 💯😂
This video is one of the best explanations of DNA Microarray I've come across on UA-cam. It's concise and efficient, and it is clear even for someone without a background in biology.
Great! I needed to watch a video about DNA microarray to understand how it works. It helped me a lot! Thank you.
From now on you can become a member of this channel! You will have access to some cool emojis like this:.
This is of course voluntary, but the financial support helps me a lot.
Hi Henrik! I just discovered your channel and I think your videos are of great help for me. Thanks for making them :)
that was brief and so useful, thank you so much
absolutely outstanding explanation. Thank you. Love from india
Really straightforward! TYSM
So simple erklärt, herrlich! Vielen Dank
Thank you🙏 very clear explaination!
It would have been very helpful if you could upload the notes of this microarray..
Explanation is amazing sir!!
This is very helpful! Thank you!
thank for making complicate topic easier to understand
thanks! Now, I understand how this analysis works
I can hear your german accent a mile away lmao
This was a amazing explanation bro thx for putting in so much work!
Amazing! Best one on UA-cam! Thanks
thank you so much you made it easy to understand
Actually it helped me a lot to understand , thanks a lot
Great video, very well explained 👍🏼
You are amazing!! Now I can understand finally🎉🎉 thank you soooo much Have a nice day😆😆
Great explanation, thank you very much
you are the best, thank you
Awesome channel, keep it up!!!
Thank you it just blessing to me cleared everything thank u so much please upload more vedios am enjoying to study from here and my concepts get more strong cause you made it clear thanks onece again
Very clear thank you!
Very helpful video:) thanks!
that was really helpful thank you⭐️
Great video man
Amazing job
Very nice, thanks 👍
Amazing!
Highly conceptional
A good explanation 👍👍
Great explanation
Thank you!
Very nice explanation sir😊
Great , Good job 😍😍😍🤩🥰
very nice very simple
well explained video, the best i habe found, but i have a question: so the oligonucleotides must be complementary to the cDNA and the cDNA has the same direcetion as the leading DNA (3' 5'), so the oligonucleotides have the complementary direction of 5' 3'? sorry for the bad english
Great stuff
It is incredible your ability to do concise videos with a lot of information and completely animated! I have just a question: how exactly does the cDNA become fluorescent? All the bases used are fluorescent?
Thanks, that means a lot!
I did some more in depth research... and there are many ways in practice. One as you mentioned is using fluorescent nucleotides (all dCTPs are labeled). Another option is: using fluorescent forward primers. This paper might help in case you are super interested in the labeling steps:
www.sciencedirect.com/science/article/abs/pii/S0167701205001806
Another possibility:
Using 3´end labeling with fluorescent nucleotides + terminal transferase
(www.future-science.com/doi/10.2144/000112182)
Super interesting and clear. Wish you could do some videos on the energy metabolism of cancer cells!
@@binebum1 I did one about the warburg effect.. check that out!
Really nice
Very great explanation, im studying for med school entry
Thank you
Nice and easy
good explanation
Great!
This is very helpful.,! Thank you!
I want to know about which Bioinformatics tool has been used To (Raw)data analysis.,
Thank you alot
🔥🔥🔥
this is very clear than the dr. explanation
Merci
Kuss geht raus, brudi
Why would the sample 2 material not binding to the array cDNA mean that the gene is not expressed? Could it not just mean, that there is a mutation (e.g. microduplication) that prohibits the probe from binding while the gene can still be translated and expressed into a protein by the cell in a „loss/gain/change of function“-mutation?
U save my 7 marks 😀
How does hybridization occur? Are the oligonucleotide probes single-strands as is the cDNA or is there a thermocycle similar to PCR?
Yes, the oligos are single stranded!
How the cDNAs hybridize with the probes. Probes are one-stranded okay, but aren’t the cDNAs are double-stranded?
Clear and concise explanation! Question: does each sample go on its own microarray, or are they mixed and applied on the same microarray? Thanks!
Each sample is typically applied to its own microarray.
However, there is also the possibility to apply multiple samples to a single microarray. This is commonly done with a technique called "multiplexing". It is done by hybridizing different labeled samples to the same microarray, or by using different probes for each sample on the microarray.
Ah, that's why it was confusing. It seemed like "sometimes". This really clarifies it. Hope it helps others too!
Hi, I got few questions
1 - we agree that we label AFTER retrotranscription during samble preparation?
2 - The oligonucleotides found in each microarray, are complementary to cDNA ? so they are DNA and not RNA ?
Hi,
1 - there might be other assays, but mostly you label with fluorescent nucleotides DURING cDNA synthesis
2 - Yes, they are DNA
@@henrikslab thank you !
Very easy😁
the health and disease sample complimentary strands that is put in one tube is from the same person or one is from the case and other is from the control?
Technically, that depends on the research question you are asking, both would be possible
Thanks for concising it rather than many slides lol.
Can we have more samples at the same time;Does this technique only work for cancerous cells or any type of unhealthy cells;
Yes, you can apply multiple samples to a microarray at the same time. This is commonly done using a technique called "multiplexing". Multiplexing allows multiple samples to be processed simultaneously on a single microarray. It is done by hybridizing different labeled samples to the same microarray, or by using different probes for each sample on the microarray.
👍
how do u make ur animation
To 95% I create everything in Powerpoint, only rarely I also use Inkscape
Is there anyone here to help me? . My master asked us to find (the mRNA -DNA ybridization use for which microbes?) Pleaseeee help me
Hybridization
Is the oligonucleotide RNA or DNA?
Rna only
Ginge das auch auf Deutsch? :D Nicht sonderlich schwer rauszuhören. Mein ich nit böse, danke für's Video!
From 0:40
Thanks for this video ! just a small point here 0:45 "tissue from a healthy cell" doesn't make any sense, you should have said cell from healthy tissue or something like that.
das ist ein sehr deutsches Englisch haha.
ist aber gut erklärt danke :)
let me guess, you are german, but nice vid
Vote park gunwook
you are German, aren't you?
He is Indian
Sound indian
It's literally in the Chanel description mate🤦🤦
Ja er hat einen deutschen Akzent, und jetzt?
The guys in the replies probably never heard an indian accent lol
😢
Du bist doch Deutscher, dann füg doch wenigstens Untertitel ein🥲
How do u apply this technique in microbiology to detect pathogenic microbes?
Thank you!
Thank you