You are very welcome! ❤ While the DNA starts as double-stranded, the Maxam-Gilbert sequencing method involves converting the DNA into single-stranded form to allow specific chemical cleavage and sequencing. Therefore, the DNA is treated to be single-stranded during the critical steps of the process.
Very good explanation Sir, but in the last place where you have show the gel electrophoresis data, there is a mistake. According to your DNA sequence, the shortest sequence should be in the G+A line of the PAGE. But,you have put it on the C+T line. Leaving this, its the best video on youtube for this topic.
Do the 4 chemicals cut on the 3' end or the 5' end or both or does it depend on the chemical? It doesn't seem to matter since the P isotope is always in 5' but I want to know
I have a question if we apply the chemical that cleaves at G wont it cleave at both G sites at the same time so a GCACCATCG be formed disrupting the electrophoresis
Hi, thanks for your video! I have a question though, why wouldn’t you just use A instead of A+G? Also, how is it possible to use one strand of DNA for multiple cleavages, since it literally requires each sequence end to be destroyed?
I'm russian, but I understood your lesson better than the explanations of all russian-speaking bloggers, so thank you
Wow, thank you! That means a lot to me. Welcome to the channel! 😊
clearest expanation of Maxam Gilbert method!
Wow, thanks! ❤️
I cannot believe i never saw you before, THANKS
Welcome to the channel! 🙌
So how do I know the first sequence as the fragment can't break down at the very beginning ending up nothing
❤ Thank you i understand everything just a question, the DNA we sequence is simple strand right?
You are very welcome! ❤ While the DNA starts as double-stranded, the Maxam-Gilbert sequencing method involves converting the DNA into single-stranded form to allow specific chemical cleavage and sequencing. Therefore, the DNA is treated to be single-stranded during the critical steps of the process.
Very good explanation Sir, but in the last place where you have show the gel electrophoresis data, there is a mistake.
According to your DNA sequence, the shortest sequence should be in the G+A line of the PAGE.
But,you have put it on the C+T line.
Leaving this, its the best video on youtube for this topic.
Excellent video mate
Thank you! Glad you liked it! 😊
Hi..thank you so much u helped alot ❤
But i have a question pls ..how do u know the very first nucleotide?! It wasnt in the gel
Do the 4 chemicals cut on the 3' end or the 5' end or both or does it depend on the chemical? It doesn't seem to matter since the P isotope is always in 5' but I want to know
Thank you for making this video.
My pleasure!
I have a question if we apply the chemical that cleaves at G wont it cleave at both G sites at the same time so a GCACCATCG be formed disrupting the electrophoresis
Hi, thanks for your video! I have a question though, why wouldn’t you just use A instead of A+G?
Also, how is it possible to use one strand of DNA for multiple cleavages, since it literally requires each sequence end to be destroyed?
wow, very nice and simple to understand. THANK YOU
You're very welcome! 😊
Thank u sir
thank you for your explanation
You are welcome!
thank you so much 💕😭
You're welcome 😊
Wow very helpful. You posted this on my birthday🥰.
So glad to hear that, Thywill! 😊
Nice
Nys one
👍
Hi