Sanger sequencing
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- Опубліковано 2 жов 2019
- This is an animated video on DNA sequencing by Sanger's method.
Sanger sequencing method is based the use of dideoxy nucleotides in the DNA polymerizing reaction. The hydroxyl group attached at the 3' end plays a very important role in DNA polymerization reaction
during DNA polymerization, There is a reaction between 3'OH group and the phosphate of the new dNTP to be added.
in 1977, Frederick Sanger had a brilliant idea. if the 3'OH group of the nucleotide is absent, then there wont be addition of new nucleotides and the reaction would stop. The incorporation of dideoxy nucleotide and termination of polymerization reaction is a random process. hence the overall mixture will have several fragements that have experienced the chain termination. If the length of each fragment is known then the location of nucleotides in the DNA can be estimated.
Classic example of some random youtube dude better explaining a concept than my professor can
funny how my professor used the video of this random dude to explain it to us
So do I 🤣
You are from which country and which college ,friend
True af😂
God bless you you're right dude...
Its crazy how things like this when taught correctly are so easy to comprehend. Professors/Teachers need to take notes from this guy!
That is 100% true. The video animation and a carefully thought-out presentation enables a good teacher to reach millions of students on UA-cam.
crazy a 6 minute video is more helpful that 1.5 hr lecture...
To be a good teacher, you also need to know how to talk. This guy failed at that, his english is so poor that you do not even know what he is saying.
tf ur talking about his english is great@@m.m.3552
Just because you have a PhD in something, DOES NOT mean you are a good teacher. Thank you so much for this my friend.
Best video on UA-cam available for sanger sequencing.
this is the best video on Sanger's Method I've seen. I finally understand it now. Thank you so much!
Me too!
i have done it at least 1000 times and i wouldn't be able to explain it better
Yes! Totally Agree! Simple and Comprehensive!
Yeah, simple best
so we're all here bc our professors can't teach anything during lecture :')
My goodness, people really weren't joking when they said you're an amazing teacher. Aside from having my mind blown from how genius this technique is, my mind is also blown from how amazing of a teacher you are. Thank you ever so much!!!
i just want to thank you, you explained this better than most of my professors during my pharmacy school years. thank you!
I mean my professor made this topic so hard that I thought I don't have the brain cells to understand this. Tysm buddy
This is hands down the BEST Sanger's Method video I've seen. So clear. Excellent work! Thank you
The most clear and thorough explanation of Sanger Sequencing. I couldn’t wrap my head around how the sequence of the strands are determined but it is thoroughly explained in this video
I have been struggling to understand this concept, and this video was perfect!!! Thank you very much.
This is one of the best science concept explanation videos I've seen on youtube in a while. Thank you for explaining this concept interestingly.
This is my go to video to brush up on Sanger's method. Thank you SO much for this!!
Saved my life, i hate not understanding something but thankfully i found this video. Great explanation!
My Microbiology exams are approaching and this video is a pure Bliss to me.This video is perfectly making my bases strong along with making me understand the whole topic which my professor failed to during a whole 1 hr lecture in my native language.
Clear, helpful, and straight to the point presentation. Well done and Thank you!
Very explicit! I wish I could like this video a million times. Thank you so much!
This is really the beeeeest video about sequencing method . very simple, very clear, wonderful!
It's insane this works for whole genomes! Excellent explanation!
Exactly what i was looking for. Thank you!
The most amazing explanation! Simple and perfect! Thanks
Easily the best explanation of Sanger's method. Thumbs up
this 6 minute tutorial saved me a full hour of time of watching my lectures, thank you.
This was amazing, especially for My MCAT prep !!! cleared up so much , thank you
had to watch it twice but this concept finally clicked for me, thank you!
So far, this is the best video explaining this topic!
U just killed it ...rather than watching so lengthy and complex videos ..its far better and easy way to learn
:-)
I owe you my firstborn child and my left testicle. Thank you for your services I am now enlightened
thank you so much for a clear and detailed explaination! Your work is much appreaciated!!!
Very nice, simplified way of explaining. Very easy to understand. Thanks
Thank you! Very helpful lecture and an amazing explanation as to how the procedure works.
your explanation is perfect thank you so much
That was amazingly Explained in a very simple manner .Thanks Dude.
Absolutely fantastic explanation. The explanation of the x-ray film method and the evolution into the electrophoretic capillary method was unclear to me in the textbook, so this saved me a great deal of time. :)
that was so concise and to the point thank you !
Exceptional presentation. I can breathe again.
This was so clear, thank you so much!
Thank you. Have been going nuts trying to understand my uni lecture and other you tube videos until I found this one.
Fantastic video! The information was presented in such a clear way that it really helped!
Phenomenal explanation! Thank you for this video it helped a lot.
Explained so nicely, and the technique itself is so brilliant ❤
Very clear and easy for understanding! Thank you so much!
YOU ARE THE BEST!! thankkk yoooouu vary vary much from deep inside for this amazing video.
This was so very well explained. Thank you!
an absolute true legend hands down.
Thank you making it easier and more understanding 😌
This clarification is sooooo cool!!! Big thanks
This is called a perfect vedio. Just amazing ♥️ I'm really amazed how someone can make us understood this hard topic so easily within only 7 minutes
Thank you very much!!! The best explanation for Sanger sequencing💌
brilliant, thank you for making this video, best explanation on youtube
So much Understanding God bless you
Thank you for your well clear explanations and well animated video.
What a great video. I understood everything
So well explained. Thank you!
Hey you deserve a Nobel in education!
Never understood this except for now..... Amazing !!
Thank you! You explained it super well!
You are the best explainer ever ❤❤
Thank you so much! I found this video very informative
The Greatest Video in History
The best video explaining sangers method.. Goodjob👍
Great simple explanation! thank you very much
Very well explained, thank you very much!
Thank you for the explanation! Easy to follow and understand! :D
I could learn this I my sleep, so easy to follow
You explained this better than my teacher. Really nice video
That was super clear and helpful !
Your video helped a lot hope you are continuing making this kinds of videos
Thanks for this video, it's the best one! Thank you so much!
very clear and concise; Thanks a lot
Now this is amazing explanation.
Very clear explanation. Thank you
Exactly what I was searching for... 😍🙏👌👌👌
It was super clear, thank you!!
Thank you for this illustrative video I totally get the concept
Thank you! That was very helpful 😊
What a great video! thanks a lot!
This is a fantastic short and simple video :)
Such a beautiful class and good explanation, thankyou ❤
i love this video, my teacher spent 1,5 hour explaining this and i couldn't get it, now I finaly understant
way better than my prof explaining... thanks so much
amazing presentation. thank you for this.
Perfect job at explaining 👌
What an explanation well appreciated 👏👏
You're a star thank you for explaining this so well!!!!! :D
Finally i got best explaination on this topic thank you very much ☺️
the best video, thank you so much man
So happy to see this video..it is much informative
This video is so informative it sort out my problem in this topic
This is an excellent video.
Excellent video, the best related with the topic
Great video! Thank you
awesome explanation and visuals
very clear explanation! thank you!
Thank u soo much ..teachers will hurry to finish their chapters and nothin would enter my brain..thanks for this video
Thanks. This video helped me a lot.
Very well explained.. thank you
Wow I have my biochem exams in 5 hours and this is a life saver
You are good at explanation. I can understand a lot from it.
So cool!! Great explanation, tysm