Its a good try, but there are few things which has to be dealt for better understanding like: 1. what chemicals are used specifically to cleave a certain nucleotide (G-DMS, A-Formic acid, C-Hydrazine+NaCl, T-Hydrazine) together with piperdine to cleave the phosphodiester bond of modified nucleotide 2. How to make single stranded DNA fragments (heat denaturation or using helicase) 3. Dimer formation are in combinations of A+G and C+T If a band in the DNA sequence appears in both the G-reaction and the G+A-reaction lanes, then that the nucleotide is a G. If a band in the DNA sequence appears only in the G+A-reaction lane, then it is an A. The same decision process works for the C-reaction and the C+T-reaction lanes. 4. What quantity of sample DNA is required for sequencing (if not sufficient, have to run PCR or do cloning to amplify) 5. What about the left part of DNA strand after cleavage (they cant be visualized, due to lack of isotope) 6. What percentage of gel is preferred (6% polyacrylamide gels are used, bcz they are too sensitive in differentiating the DNA bands with 1bp size)
I'm doing an essay on genomic analysis, and these videos on sequencing are amazing for a proper, scientific understanding of how sequencing has developed over the years. Thank you so much for the clear and helpful video!
@@shomusbiologyofficial Man I appreciate them a lot. Besides books some things aren't explained anywhere except by you. Based af that you still read comments
I am a veterinary student .....GADVASU....number one veterinary university in india......still we dont have any teacher like u sir......thank u very much❤️❣️
no need for short videos....you are doing great job... because it is helpul for begginers,,,if you start to make short videos ...then the beginners will not understand....thanks alot...very helpul.....
when you write down on board that method is good for understanding and due to only this difference between you and other you tubers i was watching your videos
Awesomely explained. This is my first video on Maxam-Gilbert, n you cleared the concept seamlessly. I won't go into technical details, I will just say that concept-wise this lecture is awesome. :-}
@@shomusbiologyofficial,your class is really helpful, sir you take an example of 12 nucleotide, and in autoradiography in the 11th portion the 12th nucleotide come..can you please explain it
Iam so happy that you replied to me I have used your site to undertand concepts for my molecular biology masters degree. At Makerere university Uganda… thank you we appreciate your efforts to teach the world science
1. Not Chemical Synthesis. It is Chemical Cleavage (two completely opposite meanings) 2. First nucleotide determination without which DNA sequencing is incomplete. From my understanding, you are supposed to initially cut/cleave the DNA sample with a restriction enzyme. From this you get to know where the DNA is getting cleaved (as you know the restriction sequence of the enzyme). This is then followed by the steps explained in the video.
thank's very much now I depend on you very much to understand any thing .I wish I Can reply the favour ❤❤ and please don't stop many students depend on you . Marwa from egypt
plzzz sir explain this in black board . its a humble request from my side because i saw this explanation in black board on many other u tube channel's. but for me sir only u have abality to explaining in black board with ease. otherwise this presentation is also very good sir👍👍
Thank you so much sir. Your teaching method is very easy and very helpful for understanding. Sir please make a video lecture on Evolutionary Game Theory.
Sir I have a question As we can see that while chemical degradation sequence is cleaved at the site just before to that perticular residue so what about A which is radiolabled as a first residue. How we will know that A is present at first becs when it get cleaved we will get only phosphate without sequence?
At 1 guanine reaction ,u told that DMS will cleave the guanine ,so no stretch of DNA will form . But how it produce 3 types of strand at guanine reaction ,because DMS will break sequence ,so we will get same type stretch DNA only sir ,???
i have a question.. why we select G and C bases separately.. i mean we took purines and pyrimidines in two test tubes n G and C in rest of the two so why not A and T??? and we treated T+C tube with the chemical to cleave T and upto some extent C as well.. so why that chemical isn't working for T?? n why the result is same for C and T+C???? reply
I just realised that I've been passing my entire uni thanks to you but never said a proper thank you so: Thank you! (=
Same 😂😂😂
Samee 🥹
Its a good try, but there are few things which has to be dealt for better understanding like:
1. what chemicals are used specifically to cleave a certain nucleotide
(G-DMS, A-Formic acid, C-Hydrazine+NaCl, T-Hydrazine) together with piperdine to cleave the phosphodiester bond of modified nucleotide
2. How to make single stranded DNA fragments
(heat denaturation or using helicase)
3. Dimer formation are in combinations of A+G and C+T
If a band in the DNA sequence appears in both the G-reaction and the G+A-reaction lanes, then that the nucleotide is a G. If a band in the DNA sequence appears only in the G+A-reaction lane, then it is an A. The same decision process works for the C-reaction and the C+T-reaction lanes.
4. What quantity of sample DNA is required for sequencing
(if not sufficient, have to run PCR or do cloning to amplify)
5. What about the left part of DNA strand after cleavage
(they cant be visualized, due to lack of isotope)
6. What percentage of gel is preferred
(6% polyacrylamide gels are used, bcz they are too sensitive in differentiating the DNA bands with 1bp size)
Praveen Kumar you are stunning.help me a lot
hello friends
Praveen Kumar Thanks
Thanks
thanks
I'm doing an essay on genomic analysis, and these videos on sequencing are amazing for a proper, scientific understanding of how sequencing has developed over the years. Thank you so much for the clear and helpful video!
You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share
Even 10 years later Shomu is somehow the only person to explain molucular biology on youtube.
Thank you so much for appreciating my efforts
@@shomusbiologyofficial Man I appreciate them a lot. Besides books some things aren't explained anywhere except by you. Based af that you still read comments
I am a veterinary student .....GADVASU....number one veterinary university in india......still we dont have any teacher like u sir......thank u very much❤️❣️
Glad to hear that you're getting benefit from my lectures
I came across this video , and may i say you are the best youtuber teacher we have got
Hands down sir, you are brilliant
seriously... The Best Lecturer Out there.. I find it so effective.
no need for short videos....you are doing great job... because it is helpul for begginers,,,if you start to make short videos ...then the beginners will not understand....thanks alot...very helpul.....
+Ejaz Khan thank you.
My best BIOLOGY teacher over whole youtube
Thank you so much for appreciating my efforts
@@shomusbiologyofficial Love From Pakistan
This is so helpful but I almost got confused at the initial result interpretation but thank God, I later understood it.
Thank you Shomu Biology
You're welcome
Thankyou sir.....you re the best teacher...😍
Interesting and very clear explanation. don't bother about the time because it's very interesting and understandable. Thanks shomu!
indeed its helpful...........complicated topic bt after watching twice /thrice i can understand for sure..
+Alwina Anam thank you very much
Really really helpful... Understood the topic thoroughly... ☺️
+mayur tupe thank you. Glad you liked my lectures
Shomu's Biology My pleasure... ☺️
when you write down on board that method is good for understanding and due to only this difference between you and other you tubers i was watching your videos
Awesomely explained. This is my first video on Maxam-Gilbert, n you cleared the concept seamlessly. I won't go into technical details, I will just say that concept-wise this lecture is awesome.
:-}
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
@@shomusbiologyofficial,your class is really helpful, sir you take an example of 12 nucleotide, and in autoradiography in the 11th portion the 12th nucleotide come..can you please explain it
Thanks for this video. It's really helpful for me.....This topic is in my syllabus....thanks a lot
You're welcome
Better than my class work ❤🎉
You're welcome
Iam so happy that you replied to me
I have used your site to undertand concepts for my molecular biology masters degree. At Makerere university Uganda… thank you we appreciate your efforts to teach the world science
you have a simple way in teaching and presenting the idea
Thank you. Glad you liked my lectures
Great sir very easy and understanding english best method of teaching 🇵🇰
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
Thankyou for the wonderful explanation sir❤❤
You're welcome
that was some top explanation man. thamks!
You're welcome
Beautiful sir...
God bless you..
🙏🙏🙏🙏🙏🙏🙏
It's really helpful.. Thank you so much
You're welcome
1. Not Chemical Synthesis. It is Chemical Cleavage (two completely opposite meanings)
2. First nucleotide determination without which DNA sequencing is incomplete. From my understanding, you are supposed to initially cut/cleave the DNA sample with a restriction enzyme. From this you get to know where the DNA is getting cleaved (as you know the restriction sequence of the enzyme). This is then followed by the steps explained in the video.
can u plz refer me any book for this topic
Dude you are the BEST! KEEP IT UP
It’s very clear nd helpful .thank you for this🙏🏻
You're welcome
Very nice sir ! no words to tell and your classes helped a lot. thank you soo much sir
Thank you so much for appreciating my efforts
thank's very much now I depend on you very much to understand any thing .I wish I Can reply the favour ❤❤ and please don't stop many students depend on you . Marwa from egypt
Thank you so much!
You helped me alot. I owe you.
Thank you. Glad you liked my lectures
Hain?
Sir your channel is v helpful . I get everything here
U are a super teacher 😍🙏
Thank you.
but what about the first nucleotide ? how to know it?
Thank you very much sir... 🙏
You're welcome
It's really helpful...
Thank you. Glad you liked my lectures
plzzz sir explain this in black board . its a humble request from my side because i saw this explanation in black board on many other u tube channel's. but for me sir only u have abality to explaining in black board with ease. otherwise this presentation is also very good sir👍👍
I love your explanation♥️
Thank you so much ♥️
Maxan and Gilbert sequencing explain very easily..
Suman sir please make video lecture on Game Theory..
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
@@shomusbiologyofficial sir please make video lecture on Game Theory
The video was quite helpful and made understanding easier. Thank you very much
You're welcome
Thank you sir for explaining it beautifully!
You're welcome
Thank you so much sir. Your teaching method is very easy and very helpful for understanding.
Sir please make a video lecture on Evolutionary Game Theory.
You're welcome. Glad to hear that you're getting benefit from my lectures
@@shomusbiologyofficial sir please make video on Evolutionary Game Theory
Cysteine.....😅 repeated twice lol
But nice explanation as always your videos are helpful keep it up👍
praveen Kumar.....thanks for adding more information
I think whole biotechnology students are graduating seeing your videos ❤
Thank you so much for appreciating my efforts
Yeah🥰
Absolutely right
Agree 💯
Thank you soo much professor 🙂
You're welcome. Glad to hear that you're getting benefit from my lectures
Great...... Thank you
You're welcome
Awesome. It helped me a looooot. THANK YOU
You are the best💜💜💜
sir carry on your vedios are very helpful
amazing video
Thank you
Thank you very much Sir. It helped me a lot
extremely helpful!!!
+samjhana stha thank you. Glad you liked my lectures
And what about first base Adenine...gel doesn't have this info.. so whats the reason sir..plzz...tell us
thank you bro i got help from this video..
keep it up
Thank you
doing a great job man (Y)
How can you tell the first nucleotide is A(adenine) from electrophoresis? Please answer
yes plz
thank you...
great explanation...
great explanation. thanks :)
Thnk u for helpful explain
You're welcome
How did you know that the very first base in the gel is Adenine?? Someone pls answer
Thank you
You're welcome
Thanks♥
You're welcome
waah sir...lovely...very easy
diagram is from which book? a good informative video!!!
Thank u so much.. really helpful
+Suprita Dash Glad it helped
simply awesome!! got it :)
Sir please do video on edible vaccines it is very useful for us.
Okay
thanks for your explain
Sir it was very helpful but how A is the 1st nucleotide is not I think explained
How can you identify A at Terminal 5 prime end. As it shows the band in T+C.
you are AMAZING
During chemical termination is the single strand added newly to each test tube everytime?
Please what is the name of the referenced book, how do we get it
Sir why we Have To elute out the heavier band and work with only the lighter band after the denaturation of DNA double-strand??
Please reply sir
Like many others I also want to know whether 1st base can be detected correctly from this method or not.......if not why
Sir I have a question
As we can see that while chemical degradation sequence is cleaved at the site just before to that perticular residue so what about A which is radiolabled as a first residue. How we will know that A is present at first becs when it get cleaved we will get only phosphate without sequence?
Thank u
You're welcome
Great work
Thanks alot👌👍
The last step is quite complicated. ..
Awsome great work love you
Thank you so much SIR
I love this guy
Sorry sir can you tell me a little beat in interpretation of result sequence if you have C and G of the same size what base will you labble first
Won't their be be combinations for cleaving out T in 3rd tube
Like 1 and 2 T
And another one 1 and 3 , 1 and 4, 2and 3, 2 and 4?
Thanks! what is the sequencing QiaSeq target amplicon method different from Sanger sequencing method ? which is superior?
At 1 guanine reaction ,u told that DMS will cleave the guanine ,so no stretch of DNA will form . But how it produce 3 types of strand at guanine reaction ,because DMS will break sequence ,so we will get same type stretch DNA only sir ,???
infact it is helpful.
Great.
i have a question.. why we select G and C bases separately.. i mean we took purines and pyrimidines in two test tubes n G and C in rest of the two so why not A and T??? and we treated T+C tube with the chemical to cleave T and upto some extent C as well.. so why that chemical isn't working for T?? n why the result is same for C and T+C????
reply
but how can we get the first base by using this method
Sir.. How can we know about the first nucleotide?
why the first base (A) is not identified..?
sir why are we unable to find the first nucleotide ?
I have understood all but why shoud we underline some particular sequences
Nice video man, what are u studying? :)
Thanks a lot.
May Allah please uuuu😍😍
Thank you
How same DMS cut each nucleotide at different tubes sir ???
Sir can you recommend books related to life science
Campbell Biology
That sorry got everyone's attention 😂