HPLC | High performance liquid chromatography

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  • Опубліковано 3 жов 2024

КОМЕНТАРІ • 344

  • @artemiskar1897
    @artemiskar1897 3 роки тому +731

    youtube once again helping more than the university instructors

  • @ArtuursPaulausks
    @ArtuursPaulausks 2 роки тому +134

    Well, as a guy who daily works with HPLC have to say video is very good for brief introduction. Obviously HPLC is more complicated, with much more details. Just want to give shout out to all chemistry students and tell them to not be afraid of HPLC. In most cases, it is easy to develope a method on HPLC to determine some kind of compound, it might take just a few tries to make sure your compound of interest is separated from rest. Just always remember to filter your samples using 0.45 um filters and inject clean samples, also use guard columns :)

    • @TrangNguyen-nw9dq
      @TrangNguyen-nw9dq 2 роки тому +2

      Dear, It is grateful when I read your comment. Could you suggest some videos which relate to developing method analysis on HPLC. I am a student who is learning HPLC to support for Master's and phD courses. Therefore, I appreciate if you could support me. Thank you so much.

    • @animaluveteranu9523
      @animaluveteranu9523 Рік тому

      don t forget to mesure you r standards corectly and shake them on ultrasonyc device It happends to my colegue and he finds out after 3 days XDDDD but I have a question for you if you work on hplc are you working on spectofotometers that use uv light?

    • @ArtuursPaulausks
      @ArtuursPaulausks Рік тому +1

      @@animaluveteranu9523 UV/VIS thermo Dionex Ultimte

    • @冯玲巧
      @冯玲巧 Рік тому +1

      Hi there, your latest post about hplc really caught our attention. We are the manufacturer of hplc and have 20 years experience, if you are interested, provide an email address and we can send you the case study proposal.

    • @helmetongrass1893
      @helmetongrass1893 Рік тому +1

      hey, uhh.... can you please tell me if he said absorbent or adsorbent material? I mean when he is talking about the material in the column...
      1:34 Edit: Nevermind, it's adsorbent material

  • @nbent4607
    @nbent4607 2 роки тому +9

    Real-life heroes don't wear capes, a man once said.
    Thanks a lot!

  • @srenathganesan
    @srenathganesan 2 роки тому +29

    Awesome explanation. No need to search for any other topic. Its a complete combo package.
    Thank you very much for making such a video.....

  • @rachmadamayanti1858
    @rachmadamayanti1858 2 роки тому +40

    Wow, a really great explanation! If this video was launched during my college time six years ago, I would have been a great Chemist who confident about using HPLC.

    • @冯玲巧
      @冯玲巧 Рік тому

      Hi there, your latest post about hplc really caught our attention. We are the manufacturer of hplc and have 20 years experience, if you are interested, provide an email address and we can send you the case study proposal.

  • @CM-yp2hg
    @CM-yp2hg Рік тому +207

    Here cuz I lied on my resume

    • @haven3911
      @haven3911 5 місяців тому +2

      How’d it go?

    • @CM-yp2hg
      @CM-yp2hg 5 місяців тому +22

      @@haven3911
      It actually went really well. It’s not that I have no idea what an hplc is but I was not proficient enough to put it down in my resume by any means. I didn’t get that particular job but I applied to a few other and within a couple of weeks I became an analytical chemist and I am very well proficient in it now :)

    • @sinchanakr6156
      @sinchanakr6156 4 місяці тому

      Hey can you help me

    • @NavjotSingh-uq7tc
      @NavjotSingh-uq7tc 3 місяці тому

      Me as well

    • @akezelb7166
      @akezelb7166 2 місяці тому +8

      Fake it until you make it 😊.

  • @cioccolatamania3622
    @cioccolatamania3622 7 місяців тому +2

    Thanks!!
    I am truly amazed by chemists and engineers that develop these analytical instruments

  • @daanstrik4293
    @daanstrik4293 Рік тому +7

    Currently working on my internship report and needed a quick refresher. This video has been one of the most time efficient summaries i've found.
    Cheers!

    • @冯玲巧
      @冯玲巧 Рік тому

      Hi there, your latest post about hplc really caught our attention. We are the manufacturer of hplc and have 20 years experience, if you are interested, provide an email address and we can send you the case study proposal.

  • @wiggywoman5184
    @wiggywoman5184 3 роки тому +10

    thank you for this video! i'm in a biochem grad program but coincidentally never took an actual biochem in undergrad, so these videos are helping a lot!

  • @Richard-kz1ee
    @Richard-kz1ee 3 роки тому +6

    This video is a life saver. Thank you very much . Importantly, I have subscribed to this channel.

  • @aryanshah1943
    @aryanshah1943 4 роки тому +20

    perfect explanation, thank you!

  • @shinchanshinchan9155
    @shinchanshinchan9155 Рік тому +2

    Thank you so much sir it's really a clearcut explanation i can't believe this almost a huge explanation is covered in 7mins 36sec

  • @akezelb7166
    @akezelb7166 2 місяці тому

    I am chemist with 7 year experience in Chromatography and this course is very useful simple clear and straightforward. Good job 👍

  • @celebritystylewatch
    @celebritystylewatch 4 роки тому +9

    Explaining amazingly giving maximum concepts

  • @cozmos3678
    @cozmos3678 11 місяців тому +3

    Brief and clear explanation... Thank u❤️

  • @mangosorbet8183
    @mangosorbet8183 2 роки тому

    This UA-cam helps me more than my teacher in the college.

  • @sashansachinda9812
    @sashansachinda9812 5 років тому +12

    superb clearly understand

  • @sirtintly390
    @sirtintly390 4 роки тому +55

    This was just randomly recommended to me. I don't even know what this is.

    • @nk_17
      @nk_17 3 роки тому

      Lol🤣🤣🤣
      I wish this was recommended to me earlier ...😂

  • @iroshasanjeewani6080
    @iroshasanjeewani6080 Рік тому +1

    Absolutely ,This was helped me to undestand about HPLC.Thank you ❤.

  • @anamariatorres6917
    @anamariatorres6917 2 роки тому +1

    The best explanation of HPLC. Thank you so much

  • @amrayyad5897
    @amrayyad5897 3 роки тому +66

    here with a typical night of due paper in post-grad after not knowing anything about chemistry for the last 3 years

  • @hope2633cu
    @hope2633cu 2 роки тому +3

    Awesome really we are learning a lot freely. Thank you so much

  • @கவிநிலா
    @கவிநிலா 7 місяців тому

    Thank you so much..With in a short time you've explained all the basic things in a wonderful manner with the help of drawings.

  • @TechsScience
    @TechsScience 2 роки тому +2

    Very well explained 👏
    Thanks for this video

  • @jhansilanka1869
    @jhansilanka1869 4 роки тому +4

    Excellent explanation i undertood very easily

  • @MB-vd6hc
    @MB-vd6hc 4 роки тому +15

    that was awesome, thanks sir.

  • @alphynisasteaphen282
    @alphynisasteaphen282 3 роки тому +2

    thank you for such excellent explanation

  • @dawixon1
    @dawixon1 2 роки тому +1

    My phone fell off and that's why I'm here, I've always been curious how the experiment I had in my physics class worked 🥰🥰 thanks for that

  • @vig7095
    @vig7095 Місяць тому

    Once again, an Indian guy saving my grades 2 days before my Chemistry Test.

  • @baskarang7618
    @baskarang7618 2 роки тому

    Within 2 hrs I have my xam. It's a 💎 for last min preparation
    .
    ..

  • @militarybrat123
    @militarybrat123 3 роки тому +20

    Osmm it's enough for exam . 🔥

  • @scimathlab197
    @scimathlab197 3 роки тому +4

    tHANK YOU SO MUCH FOR THIS SHORT INFO!! SO USEFUL AND HELPFUL!BINGO!

  • @livesteps3384
    @livesteps3384 3 роки тому +3

    described very well.Understood the content clearly.Thank you very much.Plz do more clear lessons.

  • @ELICE6
    @ELICE6 3 роки тому +3

    Great video and very simply explained.Thank you so much!

  • @iddah3732
    @iddah3732 3 роки тому +4

    Very well explained. I can now answer any question in HPLC with ease.thank you

  • @drashtidarji
    @drashtidarji 10 місяців тому +1

    Amazingly explained!! 😍👍

  • @sabinahasanova7198
    @sabinahasanova7198 2 роки тому +1

    SO SIMPLE AND INFORMATIVE! THANK YOU

  • @christophergoudarzi9992
    @christophergoudarzi9992 Рік тому +1

    Great video as always, thanks so much!

  • @Hozanaa
    @Hozanaa 5 місяців тому

    Thanks, it was very clearly explained and specific. Neat diagrams and good presentation. Easy and not messy ❤

  • @mahakrathod8679
    @mahakrathod8679 2 роки тому +1

    Best video on HPLC

  • @AA-gl1dr
    @AA-gl1dr 2 роки тому +1

    Excellent video, thanks!

  • @deeppunch28
    @deeppunch28 3 місяці тому

    It's truly very helpful to me. I am learning from this just before my exam. Lot's of love from India ❤🇮🇳

  • @tanvirahsan5775
    @tanvirahsan5775 3 роки тому +3

    Outstanding, Thank you ♥️

  • @setupatel1476
    @setupatel1476 10 місяців тому +6

    Back ground music is not needed ,,it disturb the understanding

  • @roroiyaka
    @roroiyaka 2 роки тому +1

    very helpful, thank you for making the video. much appreciated

  • @ulamgnuszewska
    @ulamgnuszewska 2 місяці тому

    now I understand! You're a great teacher :)

  • @AS-zf4nj
    @AS-zf4nj 3 роки тому +2

    You're the real MVP.

  • @shirinmittal3225
    @shirinmittal3225 2 роки тому +1

    Very nice explanation. Thank you

  • @anikislam8507
    @anikislam8507 4 роки тому +8

    love this channel. from bangladesh.

  • @42najibshaikh98
    @42najibshaikh98 2 роки тому

    It's just awesome. Shorter video , better explanation.

  • @EnglishExcel2208
    @EnglishExcel2208 4 роки тому +1

    Nice xplanation

  • @luizantoniodavila47
    @luizantoniodavila47 3 роки тому +3

    Bastante útil para abordagem preliminar sobre HPLC

  • @Abdulkader-sq3qo
    @Abdulkader-sq3qo 2 роки тому +1

    Very well described.

  • @chennamsettiprasad7257
    @chennamsettiprasad7257 Рік тому

    What ye class explanation,,great video

  • @aprajita629
    @aprajita629 4 роки тому +1

    Thank you so much. Very nicely explained with animation & way u speak. Plz keep it up.👍👍👍

  • @samueld77
    @samueld77 3 роки тому +1

    Great video

  • @MayankSingh-uj6hi
    @MayankSingh-uj6hi 4 роки тому +1

    Really awesome videos

  • @shaikvaseema6535
    @shaikvaseema6535 5 місяців тому +1

    Awesome

  • @myworldofinstrumentation6523
    @myworldofinstrumentation6523 3 роки тому +1

    Helpful information

  • @LinitedfiA
    @LinitedfiA 3 роки тому

    Lmao. I still did’t get it why my class need 8hour to explain HPLC instead of 10 min. Good work btw really appreciate your work ,very helpful for overview.

  • @shaikabdullah..
    @shaikabdullah.. 3 роки тому +1

    Good explanation

  • @samuelbabalola3479
    @samuelbabalola3479 3 роки тому +1

    This was super helpful!

  • @shabinmillet9261
    @shabinmillet9261 Рік тому

    explained very clearly and nicely

  • @NannannanSJN
    @NannannanSJN 3 роки тому

    Thanks for video. It helped me to understand HPLC!!

  • @pepelaugh5825
    @pepelaugh5825 3 роки тому

    Really really thank you for the video
    The very best explanation

  • @rowidakhalily5760
    @rowidakhalily5760 8 місяців тому

    Awesome explanation,Thank you so much

  • @jacktam5855
    @jacktam5855 3 роки тому

    You saved my life. Thanks man

  • @dilshanchathuranga6962
    @dilshanchathuranga6962 9 місяців тому

    Thank you very much . This video was very useful for my exam❤️

  • @ponmadasamym8469
    @ponmadasamym8469 Рік тому

    Really easily understand

  • @iannalins2369
    @iannalins2369 3 роки тому

    this video helped me so much for a apresentation, thank you so much!!!

  • @casper2917
    @casper2917 3 роки тому +1

    Thanks for the explanation

  • @rajuanarase2465
    @rajuanarase2465 3 роки тому +1

    Nice explain

  • @adnanjamil9429
    @adnanjamil9429 2 роки тому +1

    Thanks of bunch

  • @ahmadhamza6618
    @ahmadhamza6618 4 роки тому

    Best vedio to understand hplc

  • @RylanVG
    @RylanVG Рік тому

    Great video, up voted.

  • @_archana_4147
    @_archana_4147 3 роки тому +1

    Superb!!! 🙌

  • @pharmaboy1510
    @pharmaboy1510 3 роки тому

    Love this Channel From Bangladesh

  • @Ocyon
    @Ocyon 3 роки тому

    Good video! Thanks for that :) Cheers from Germany

  • @Lia_liah
    @Lia_liah 3 роки тому

    Thank you for the vedio and by this vedio i understood the topic very clearly

  • @hafizghaniullahsafi6853
    @hafizghaniullahsafi6853 2 роки тому +1

    Well explained 🧡

  • @marselstomez6999
    @marselstomez6999 3 роки тому +1

    really thanks

  • @ahmadfares5243
    @ahmadfares5243 3 роки тому +1

    Simple and clear thank you 😊

  • @gandhamgracy4937
    @gandhamgracy4937 3 роки тому +1

    Easy to understand 👍🏻

  • @anchorsreyashi
    @anchorsreyashi 2 роки тому

    Very nice explanation...thank you

  • @yesplatinum7956
    @yesplatinum7956 2 дні тому

    Ty so much this made everything so much clearer. Didn’t appreciate the accent at first (I am allowed to say that because I too have an accent, a much sillier one) but towards the end it actually started sounding kind of cute. Is it like German or something or am I much worse at detecting accents than I already thought I was?

  • @Serwada237
    @Serwada237 8 місяців тому

    The best lecture here

  • @durvollagayathri12
    @durvollagayathri12 Рік тому

    Super video

  • @thewwefan57
    @thewwefan57 4 роки тому

    That minecraft noteblock beat at the end tho, lit

  • @brennanhilsher9276
    @brennanhilsher9276 2 роки тому +1

    For normal phase hplc, why would you use a non polar solvent and a polar sample? I get the point is to separate them, but non polar solvents shouldn’t dissolve polar compounds

  • @esraaalmasoudy4630
    @esraaalmasoudy4630 4 роки тому +1

    This was helpful. Thank you sir.

  • @cheneecleveland4588
    @cheneecleveland4588 3 роки тому +1

    Very helpful review!

  • @btsforever5123
    @btsforever5123 3 роки тому +1

    Tq so much sir it's easy to exam preparation😇

  • @martinnrrekjrgrndahl3715
    @martinnrrekjrgrndahl3715 3 роки тому +3

    Nicely done introduction to the subject. Feel a little more confident next time I am applying for a job mentioning this technique.

    • @nk_17
      @nk_17 3 роки тому

      You got the job?

  • @narmeenfatima5145
    @narmeenfatima5145 2 місяці тому

    thanks alottt it was very helpful

  • @kalyanirajalingham1286
    @kalyanirajalingham1286 2 роки тому

    Fantastic video!

  • @HarukaKaku-l7p
    @HarukaKaku-l7p Рік тому

    I like this video so much. If you don’t mind, I’d like to ask your permission to share this video to the other website in China for the embarrassing reason that UA-cam is blocked form accessing in China. Of course, I’ll give sources of the original website. Thank you so much.

  • @abdulrahman-ob5wx
    @abdulrahman-ob5wx 10 місяців тому

    Thank you soo much for helping us

  • @amanisaadeh
    @amanisaadeh 4 роки тому +1

    Great efforts thank u

  • @mosfiqurrahman23
    @mosfiqurrahman23 Рік тому

    Thanks. You saved me

  • @anthonytan6143
    @anthonytan6143 6 місяців тому

    Amazing video THANK YOYUUUU