Gas chromatography | GC

Gas chromatography is a chromatographic technique used for the separation of
volatile compounds. The volatile compounds are the compounds that easily gets
vaporised at room temperature. Separation occurs based on the interaction of molecules with the mobile phase and the stationary phase. The less volatile molecules interact more with the stationary phase and moves slowly
where as more volatile molecules interact more with the mobile phase and moves fast. When the sample is detected the computer gives a peak with respect to the retention time of the sample.
The area under the peak gives information about the concentration of the sample.

[@prangyadas5127]

A detailed information in a very shorter period of time and was explained in a crystal clear manner. Excellent.

[@pepe6666]

oh dude thank you. finally a way to understand this. the terminology of a gas chromatograph is used so much with mass spectropy that its difficult to figure out whats what. i had always thought those peaks on the chart were speaks on a spectrum! this has cleared a lot up for me, thanks.

[@sayurirathnayake48]

Thanks a very lot.i studied A to Z clearly from this video.it's amazing how you explain them simply.please make more & more videos.Good Luck

[@prayoga4419]

Very brief and to the point. Love it. Thank you so much.

[@tuffel03]

Thank you this was the exact format and presentation I needed

[@trevorndhlovu7834]

COOLSOME data.It's awesome staff you arranged here.

[@henrycalhoun1119]

thanks dude, very clear and helpful!

[@mustafeabdiazizismail3771]

Thanks 🙏 to your efforts, we finally understand the different types of chromatography

[@sarahkamau421]

Very well done! You're really helping us beginners! Thank you!

[@sydneym3251]

great video, great explanations, and i love your drawings/ animations!

[@Rootfresh_channel]

Last sem exams I prepared some of difficult topics from your channel and got a 86/87 marks..

[@thatvid.6628]

WOW! I am still repeating.

[@daredevil6117]

Wow online me itne marks.😜

[@sarahholland5980]

this is amazing. so simple and clear. thank you!!!!

[@sequeira7330]

summary of the video:
GC is used for the separation of volatile compounds( compounds which are easily vapourized at room temp)
Components of GC:
1) SAMPLE is mixed withe a highly volatile compound like Hexane, cyclohexane, etc.
• Long coiled column: can either be a packed column(made of glass/stainless steel) or Capillary column (made of fused quartz).
this column is placed in a CHAMBER in order to facilitate a uniform temperature. (HIGH TEMP)
During analysis, placed at 150-300°C
• the SP is packed in the column. it is usually made of silicon grease or wax as it can withstand high temperature
• Presence of a SEPTUM to inject sample just before the chamber - the temperature of the injection region is 20-50° higher than chamber. this is to facilitate the rapid vapourization of the molecules.
2) Mp: usually helium / nitrogen (inert/unreactive gases are used) are placed in a cylinder connected to the Column via the Molecular sieve.
3) Molecular sieve is used to filter out unwanted HCs, Water vapour, oxygen etc that may interfere with the sample during experimentation.
4) Detector

[@sequeira7330]

Detector - common: FID ( FLAME IONISATION DETECTOR ) which has 3 inlets:
• carrier gas from the column
• hydrogen
• oxygen
ignitor ignited the H2 and O2 to produce a flame.
when sample molecules reach the flame, they get ionized and electrons are released.
across the flame, there are 2 electrodes ( cathode for cations, and anode for anions)
the electrons generated are detected by the electrodes in the form of current and it is amplified by the amplifier/Computer.

[@sequeira7330]

the computer then generates a graph:
X axis- time
y axis- current
based on known samples and comparing their output with the output of the unknown samples, the retention time/concentration can be deduced

[@nh-vt5ws]

​@@sequeira7330 seems like you're a technician of GLC...
Excellent explaination bruh🎉

[@chandrashekarn5376]

Good explanation sir with animations 🙏🙏

[@aga4966]

Really good video, made me understand GC!

[@bankthanadorn4598]

Well explained, better than my lecturer tbh thankss

[@rimm2592]

I like these videos. They just make my life easier. Thank you so much for the explanation!!!!!!

[@table2790]

You are absolutely amazing! Thank you So much :))

[@fantasticcreativity6136]

O my God,,,such an amazing explanation
Love it,,, clear my all doubts and confusion

[@softvibes4811]

This video totally cleared everything up, thank you so much

[@Boooommerang]

Excellent video! Thanks

[@Carmelo12320]

Thank you exactly what I needed.

[@sibghatkhan3986]

Stupendous!! It is great effort
You have make it clear as crystal 🔮 mirror

[@temmietemmieson6756]

Really well explained thank you!

[@MrKpy86]

I work for a company in the Chemistry industry that manufactures instrument and that was pretty accurate, good job. Only thing is inlet is at 20-50C higher than the max temperature reached by the GC and there is also an inlet of Makeup Gas for the FID which is usually to compensate the fact that nitrogen or helium coming from the column is usually much lower than the other gases. Other than that, great video

[@shynosalexander9200]

This was very helpful. Thank you :)

[@divya.t.george6030]

very well explained understood it greatly

[@mdraselmiah3403]

good explanation and it will help me more..thanks

[@hajrabarool6044]

This video made my day today

[@themoreyouknow585]

Amazing
I really appreciate it

[@hysysplusunisimandchemcad4499]

Excellent Work, Thumbs Up.

[@JERKINSRICE]

Amazing! Very well explained. Thank you very much.

[@triciajones6923]

So very good.Thank you!

[@Ara2061]

thank you.. .thank you... thank you

[@user-yt2yl3fn4z]

Respect 🙏 from Africa Zambia better than my lecturer for a year thanks I know love this course❤

[@AntonioAdame1]

Great vid. Thank you

[@palanirajramalingam1131]

WELL EXPLANATION EASILY UNDER STAND THANK YOU

[@abrarrakin1757]

superb man, thanks a lot

[@imtme]

thank u, u are so helpful, more helpful than my lecturer

[@sabahedayati4474]

Thank you🌱💙

[@wen6519]

That was helpful, thank you for sharing

[@rabiaameer8588]

Amazing lecture clear my all ambiguous points about GC

[@faizakainat9427]

impressive..beautifully explained

[@esterindahkusumaputr]

Thank you for your video, it's clear

[@ValeriaGobjila]

thank you for this informational video on behalf of all science students :)

[@carmempacificodasilva4799]

thank you so much!!!

[@satyasurya8923]

Thank uuu sooo much for this wonderful explanation 👏👏👏

[@mustakim2144]

Thank you!

[@dimitrikolotauri6622]

thank you sir.!

[@michaelnowland1687]

Thank you

[@jono2167]

Excellent video. Very clear and informative. Good job mate!

[@stellalesirite4059]

Thank you for this🙏

[@sujikumar7831]

Super..very useful.. simple

[@user-wt9gv3kd3q]

Thank you!!!!!

[@shourovhossain8498]

Amazing.....the video clear the concept of chromatography.

[@mariofelipequevedolozano3659]

Thanks a lot!!!

[@nargesmaqsudi62]

Thanks a lot

[@jayliu7786]

i have a organic chemistry lab tonight and hoping that it goes well. thanks for the video, it's very insightful :)

[@muhammadammar6262]

very well done video very good keep it brother

[@_sri_702]

Thank you 👍

[@arbazchaudhary5752]

thanks for this video you explained
very well

[@sanjayg114]

In fid carrier gas was nitrogen/ helium which carries the sample to burning area?

[@zahidhussainzaigham9223]

Well explained briefly. Thanks

[@ianedmonds9191]

Excellent. Thanks for that.
Luv and Peace.

[@khushbujha4957]

As we can say in 6min our separate sample is there??

[@bhaveshthakor4219]

Amazing explanation

[@BrainKhan]

Nice one explanation i always found there

[@juliana_jesus]

Thank you very much!! May I have the references for further reading please?

[@dinosanchez8528]

Good video, thanks

[@baochau4877]

thsnk you so much, very helpful

[@ianmatthewkline8279]

Okay but what about when two samples have similar or even the same retention time? I use LC-MS/MS as a main tool for my PhD work but I was curious about how this other technique works.

[@1987Wulf]

You can use a different phase to achieve different RTs. There are some that will never work on GC. For instance, M,P-xylenes are not chromatographically separable. However, using a HRMS will differentiate the identifications.

[@mumizashahid6047]

Very very very very helpful
Thanks alot for this

[@9madhumitha928]

Very useful ☺️👍 thank you

[@bijiteshchoudhury8274]

Well explained
Thank you

[@kamnabharati2970]

Thank you Sir.❤️🙏🏻

[@yasminarijah1646]

Thank you. Very helpful

[@jignasachiramana8269]

really helpful !

[@alaulislam1869]

Brilliant explanation dear sir☺️❤️

[@praveenkumarkp4084]

Very helpful 👍 plz do classes of HPLC and spectrophotometer

[@aminechahid2004]

THANK YOU

[@j.d.tgaming7199]

Hiee

[@hisanathasni5499]

Thanks bro ❤

[@user-dq9ty5tq1z]

thank you sir for your good explaination.

[@user-kt3xu3ml1q]

It's really helpful ...thanks 💜

[@ankitagaonkar4298]

Wow explained well

[@fancytracy]

How much of the sample is put in the column?

[@nihalanasrin9598]

Highly useful

[@laylachemist5245]

Well done sir

[@pharmasolutionpakistan7668]

Good explaination

[@kooky434]

Amazing :D

[@seeker8906]

Thank you Sir

[@venkatk669]

Good information

[@Matteo-mx1sz]

thanks

[@muhammadasifresearchschola6620]

can we get these slides in PDF?

[@mairaseiilgazy7510]

Thanks

[@user-jd3bk2wq6m]

In bad need for applications of gas chromatography 🥺💔

[@spurthiblk3306]

tq ...Vry useful

[@muskanagarwal1173]

Amazing sirr

[@medotedo8410]

My teacher says that GC is rapid faster than others (Column chromatography and HPLC) is that correct ?

[@kamnabharati2970]

Yes. Analysis is done within minutes or even sec.

[@b4baigan989]

Wow... Just wow

[@eswarnihargaming673]

Nice.. Keep go head...