Thin Layer Chromatography (TLC)
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- Опубліковано 13 лип 2024
- We know how to perform extraction, which can separate compounds on the basis of differing solubilities. But what if they have the same solubility? Well there is another technique that utilizes differences in polarity, and it's called chromatography. There are many varieties of chromatography, but the best way to introduce the concept will be to look at thin layer chromatography, or TLC for short. Check it out!
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You just summarized our 3-hour lesson into 9.5 minutes
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He is my hero chemist. I love orga.che. lab because of him
7:52 "It’s usually difficult to predict what will work best, so just try something and see what happens. " professor dave motivates
So much easier to understand than our lab manuals. Thank you!
Getting ready for the MCAT exam. Your videos are THE BEST on youtube for all of ochem and gen chem material! Thank you so much!
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I don't know why o-chem lab manuals have to be mystery novels. Thank you for your videos!
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DAVE. Keep up the good work. Thank you so much for your contributions. You really explain things in a manner that is so straightforward and easy to follow. I appreciate you so much.
Mahal na po kita Professor Dave. Ikaw po ang pinakadakilang guro na nakakapagpaintindi saakin ng mga bagay na mahirap intindihin.
I'm happy you've decided to do youtube... Some of us watching you right now will become great chemists in the future, and you'll be one of the main reasons we'll manage
Since we’re on quarantine my Organic Chem lab professor is using your videos and basing the work he gives around your videos
This honestly seems like an issue of legality and compensation. The situation is the same at my university (however not for this video), I don't think your school is writing checks to professor Dave... but they are certainly getting their monies.
i wish mine did that
@@danielmeaders4005 it's probably kind of new legal ground to be honest. Before quarantine happened and e-learning became almost the de facto standard in most places, using internet resources so heavily in class was probably very limited. I'm not sure ad revenue really would be fair compensation for that type of usage.
thank you! prepping for lab quizzes using your videos bc they are so much easier to understand than our manuals
I'm an msc student and you helped me pass my enterance exam and now helping with my final exams in grad school, you're a life saver! :')
great for visual learners, finally a video that makes it easy to understand. thanks.
Incredibly easy to understand thank you so much!
always satisfied with your explanation prof., a huge fan of you from India
This has changed my life
Thanks a lot man! Our schools told nothing about how this is typically used as an analytic technique
well and a simple-explained tutorial. tnx, prof.!
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At the morning of my exam .. soo helpful a huge thanks to u ❤
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U r unique .i take lab abt tlc anc CC and you make it easy and clear thank you. I wish you good health and long life
man that was a great video!
Being a visual learner instead of trying to understand material that needs to be read, I feel that this video on TLC should be shared with students that would like a better insight/understanding on this topic. From watching this video I actually learned that the TLC plate itself has hydroxyl groups that is dipole-dipole, allowing it to interact with other polar substances.
Thank you dude, it helped me a lot
well explained! Thank uuuuuuu.
شكراً جداً بسيط و مختصر ❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️
in professor dave we trust
Excellent...... thanks a lot
Thanks professor
I loved watching this TLC 👍
I came back to this a few times haha... great vid
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very excellent 💙💙
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Thank you 🙏
thankyou, it help me so much.
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Wow what a haircut Professor! You look good either way lucky you.
Nice easy to understand thaks
Brilliant!
Good personality with hair cut,by the way vedio is help full............
Thank you!
Just Thank you ❤
Hello Doctor
I am now working on an experiment, which is to prepare Schiff bases and run by escalation with the condenser for no less than 6 hours, and I want to follow the end of the interaction with the technique TLC (The solvent used for the reaction is ethanol
The question is what polar and nonpolar solvents can be used in chromatography and what ratio shows the endpoint of the reaction )
thank you so much! I just wanted to ask, do you benefit more from patreon or from UA-cam channel memberships? I really appreciate your help, this explanation was so clear
Patreon takes a smaller cut so that is much better, thanks so much for your support!
Hello Dave, please talk about Keto Enol Tautomerism
check my tutorial on michael addition
If only this video came out a few years ago... I wouldn't have looked like such a fool in my research lab haha
thank you thank you thank you
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Thank you
What solvent would you use to prepare the samples for cannabidiols. Thank you for your class! Love learning.
Thank you for the video! One question: If you have no clue what your sample is made of, how do you know whether to use UV light, or a stain to visualize the dots? Also how do you choose the right dye? Also, what do you do if some components of the mixture only get stained by on stain, while other components only get stained by another stain?
Is there a huuuuge amount of different stains the organic chemist has to experiment with or are there just a few that will cover pretty much most chemicals?
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is the polarity of the samples the only thing that impacts how far each of them will travel, or does their relative mass also have an impact?
nice
Will you be doing HPLC/GC tutorials?
i hadn't planned on it, but i'll put it on the list! it is a big one.
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4:56 just marking for my upcoming university course
So what is the typical solvent used for TLC? would that depend of the sample/compound to be analyzed?
in the lab i would typically use a mixture of hexanes and ethyl acetate. it's kind of personal preference to an extent.
I had a doubt.... how to know what component has been seperated...and also what is the use of rf value
A day before jee mains 2024
Kya mtlb Indian log smart rahte hai
what are the methods or ways of separating plant pigments?? only TLC and paper chromatography ?? please I need help
If the solvent is polar, then the polar molecules will have a smaller Rf and non polar molecules would have a larger Rf? Or is it the other way around?
yes you have it right!
Hello. This might be a dumb question but why does Silica (SiO2) have hydroxy groups?
superb presentation. and good of you to cite how TLC is a stepping stone to column chroma'
... and to work out the solvent(s) x:y ratios w/ TLC for upper half separation.
eager to see the col. chr. vid's on any time-correlation in top to bottom flows vs TLC.
and how you go about collecting the eluent with the separated product and nothing else.
also will be listening for info on the details of how one chooses a silica mesh size. and possible re-usability of silica for those of us who know how costly that stuff is.
and lastly how to choose column size vs number of grams of material to be separated.
Sir my compounds are non polar compounds. so which solvent do I use for dissove the compound and what solvents do I use in the mobile phase? sir can you tell me a little bit? I am facing a lot of problems in tlc checking
Sir why do we use organic solvent(toulene) as mobile phase in thin layer chromatography ?? Pls reply for this sir
what else would we use?
Excuse me, do you know how to prepare silica gel to absorb moisture or silica gel to act as the stationary phase in thin layer chromatography. I need to make my own TLC plate
Hmm no I've never made plates before. But check the tutorial that follows this one on column chromatography, it may help a little.
Sir How can I separate a mixture with 37 compounds in it using column chromatography
Why do you need a capillary tube to place the solution mixture on the plate? Can't you just use a pipette or dropper or something?
the capillary tube is much better for applying the solution because you press it against the plate and it is adsorbed, a dropper would be terribly imprecise
@@ProfessorDaveExplains Thanks for clarifying.
I’m confused to why the dots are not being places with marker but are placed with a capillary tube ??
the capillary tube loads the sample onto the plate
👍🏻
Please what mean tlc its same column ?
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what is the point of the mobile fase? we have to optimize on the mobile fase in a project, but what does it do exactly?
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TLC is so boring 😩 HPLC is way better 👍🏽
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Thank you
Arabic