HPLC chromatography
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- Опубліковано 5 жов 2024
- HPLC chromatography lecture - This lecture explains about the HPLC chromatography technique in a nutshell by Suman Bhattacharjee. HPLC is performed to separate organic and biological compounds using solid stationary phase.
High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent which is known as the mobile phase at high pressure through a column with chromatographic packing material known as stationary phase. The sample is carried by a moving carrier gas stream of helium or nitrogen. HPLC has the ability to separate, and identify compounds that are present in any sample that can be dissolved in a liquid in trace concentrations as low as parts per trillion. Because of this versatility, HPLC is used in a variety of industrial and scientific applications, such as pharmaceutical, environmental, forensics, and chemicals.
Sample retention time will vary depending on the interaction between the stationary phase, the molecules being analyzed, and the solvent, or solvents used. As the sample passes through the column it interacts between the two phases at different rate, primarily due to different polarities in the analytes. Analytes that have the least amount of interaction with the stationary phase or the most amount of interaction with the mobile phase will exit the column faster.
This lecture explains the following things about Hplc chromatography -
1. Hplc chromatography principle
2. Hplc chromatography instrumentation
3. Hplc chromatography types
High-Performance Liquid Chromatography - Other HPLC Types
Ultra High Performance Liquid Chromatography (uHPLC):
Where standard HPLC typically uses column particles with sizes from 3 to 5µm and pressures of around 400 bar, uHPLC use specially designed columns with particles down to 1.7µm in size, at pressures in excess of 1000 bar. The main advantage of an uHPLC is speed. These systems are faster, more sensitive, and rely on smaller volumes of organic solvents than standard HPLC, resulting in the ability to run more samples in less time.
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Thank you for watching HPLC lecture
Got the degree because of you❤😁 thank you sir... from sri lanka
All the best
@@shomusbiologyofficial ❤💪
You explain this better than any of my university professor's ever could. Thank you.
Yeah ..... same here
really
@@faisalali-mz5hs in many universities teachers are lazy
Same
You must be a back bencher lol
Better come to our university and teach our staffs how teach the students...Really your teaching is good
Thank you. Glad you liked my lectures
The best explaination on HPLC, ever. I have never heard of any explaination better than yours. I mean my professors dont teach us like that. Thank you, sir.
Glad you liked my lectures. Please subscribe and share
omg your the best dude, not even my professor explained it this well. Never stop bro, consider yourself a life saver!
I am a MSc microbiology student, I pass my degree bcz of your classes .Now also I used to watch your classes.Thank you very much sir😊
Thank you
Hlo sis I want to do msc biotech which clg is best
Wow, thank you so much 😭😭you saved my life to think I have an exam in 3 days ❤️❤️ ❤️🙏🙏
All the best for the exam
Comrade, your explanations are very simple, well organised and detailed at the same time. You are one of the best teachers on UA-cam about chemistry.
Thank you so much for appreciating my efforts
I love this tecnology , my final proyect of university was about ultra performance chromatography liquid- spectromatry mass UPLC -QTOF for detected some specific monomers of cutin in fruits , now I follow your job in UA-cam I like
Just 5 days to go for our university sem exam..and thanks to god..We have you here with us😍
I've hard problems understanding this topic. Shomu's made it all simple. I will stick by these lectures. Thanks a lot.
You can think of the interaction between the sample and the stationary phase as someone stopping to chat with a person they like, which delays them from getting to where they need to be. If you do not like the person, you do not stop to chat and speed past them/avoid eye contact (ei. Hydrophobic molecule zooming by hydrophilic stationary phase.)
Nicely described. Will it be possible to develop and describe a vedio on Glycated haemoglobin ( HbA1c) chromatogram ?
Thank you for the all of your explanation videos..most of our university badgemates watch your videos to understand Biochemistry lessons.Thank you From SriLanka❤
i always go back to yr vid sir since study and now even for my job. thank u so much! best online teacher ever!!
You're welcome. Glad you liked it
You are the best teacher 🧬💕
Thank you so much for appreciating my efforts
You are explaining complex things in very simple way . I learned a lot thanks a lot..
Thank you. Glad you liked my lectures
I wish..you were my teacher in my college...
Now you can learn anytime from my lectures
you are eplaining HPLC chromatography much better than my proffessor at university , tanks for that.
You're welcome. Glad to hear that you're getting benefit from my lectures
My favorite teacher, your lesson really helps me a lot. Keep doing so bro. Being a teacher is your precious gift.
Thank you so much for appreciating my efforts
it is always wonderful to learn from you. may Allah always bless you Ameen
You're welcome. Glad to hear that you're getting benefit from my lectures
Appreciate your clarity of thoughts .
Your efforts to promote science thro lectures like this amazes me .
Keep it up and continue your good work .
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
This video is HIGHLY informative and extremely clear. I understand HPLC now, more than I ever have. Wow! Thank you for taking the time to help us all. :)
Tomorrow is my end semester exam and u are helping me a lot with this thank u sir
You're welcome
Amar puro MSc ta tumi bachiye dile. ...thank u a lotttt💐
You're welcome
No one can't replace u sir...great lecture...keep doing, thank u sir.
you have told the traditional and the reverse phase chromatography as same...the mobile phase as philic and stationary phase as phobic....while explaining... please do check and tell what exactly the difference is
thanq..this video helped me a lot
I FINALLY GOT IT! YOU ARE A LIFE SAVER!
Thank you
Thank you so much 😊 Sir 🙏.
Your video classes are not my first options or last options They are my Only options 🙏
These are Very much helpful.
So many good wishes for u Sir🙏.
Thank you so much for appreciating my efforts. Stay tuned for more
One of the best, if not the best, video for HPLC i've watched. Thank you so much.
Very clear about the topic. The best lectures I attend ever 🙏
Glad to hear that you're getting benefit from my lectures
My university professors don't even explain...you tube is my only professor...thanks to technology and all the professors who are uploading their lectures here...❤
Thank you so much for appreciating my efforts. Glad to hear
GC is mainly used for volatile and semi-volatile analytes. Biological samples are not ideal for GC-MS not because they are vaporized but they can't be vaporized. Rather, biological samples are thermo-labile and degrade at high temperature in GC column. But, process such as derivatization can enhance volatility and thermo-lability of biological analytes such that you can analyze in GC-MS.
not all heroes wear capes.. thank you for your efforts
Method of teaching is awesome
Thank you so much for appreciating my efforts
Thank you very much .you have explained this technique better than anyone else ☺
Thank you so much for appreciating my efforts
Thanking you alot for saving us from boring lectures of our Professors....you are the saviour of millions of the students like me who have not Studied at all during the whole year becoz we knew that we have our beloved teacher Shomu sir on UA-cam to teach complicated things just like alphabets. We really appreciate your efforts for making Science easy and lovable.... thnakuhh 😊
@Sagar_University
Thank you so much for appreciating my efforts to help
You saved me too many times that i can count. Thank you ❤❤
Thank you. Glad you liked my lectures
I understand HPLC because of you. Thank you.
same with me
Very nicely and in a very simple language it is explained the complex system.
systematic and simple approach to topics makes it easy to absorb...thanku
Thank you. Glad you liked my lectures
Your explanation is really nice. Basic concepts was clears specially hydrophobic and hydrophilic. Thank u so much
You're welcome
Tq u Sr u r clases are very help full to undstand than our lecturers....... Tq u so much sr
Thank you so much for sharing the information! I've been to class and go through the notes given but still I couldn't get in but after I watch this video it really help me to understand the principle of HPLC! Very good explanation and easy to understand!
You are explaining a very nice n clear n easy to understand..thank you
You're welcome. Glad to hear that you're getting benefit from my lectures
last step of instrument is very helpful to clear confuseness
so again thanks a lot
I never attend any lecture on HPLC . But iam good at it. 👊👍👍 Waters HPLC+Empower 3 ❣️🔥 good job bro.
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
you are really awesome brother i will not able to express my all thank you for you...
Glad to help you out
love following videos even not needed for now..
Thank you
Thanks!
I really liked the way you explained it. Plain and simple but very informative at same time.
+Dinesh Kumar thank you. Glad you liked it
Thanks sir for being the reason to face the exams 🙏
You're welcome
Thank you very much, Suman! You explain very well!
Especially the one with the Gas Chromatography lecture. It is very interesting and understandable thanks to you. You know how to teach propely.
u make the things very clear and easy. thankuuuuuu so much sir
Amazing explanation about HPLC . Very clear concept with basic cover .
Thank you so much for appreciating my efforts
i love the way you explained.. i nver find it so easy.thanks
AoA sir i am really impressed by the way u teach chromatography.its remain very helpful for me and clear my doubts, sir thanks alot
Shomu, we will never be able to thank you enough ❤
You're welcome. Glad to hear that you're getting benefit from my lectures
🙏🙏🙏🙏🙌🏻🙌🏻🙌🏻🙌🏻best one
Thank you so much!!! This video made studying this topic so so easy! Thank you so much...
Glad to hear that you are getting benefit from the videos
Splendid! I didn't need to go through my lecture notes in detail. Thanks
Thanku sir... thanks a lot for this video, it helps me a lot.....keep going and keep shining🎆🎆
Very nice explained!! I like it so much !! Very nice study I'm proud of sir !!!God bless u !!!
i wish we could get you as a professor for our class :)
thanks alot sir
Glad to hear that you're getting benefit from my lectures
@@shomusbiologyofficial love you sir May Allah give you success in every part of your life
Thank you soo much sir ... u can explain very easily .. very helpful to me..
Thanks
Thanks!! What you have mentioned in video is really detailed. And I do need this.
+sabrina run thank you. Glad you liked my lectures
Best explaination..far better than my university lecturers..
Thank you so much for appreciating my efforts
Very excellent explanation u explain each and every point Hplc very good.
Glad to hear that you are getting benefit from the videos. Please subscribe and share
Hi Shomu, i just Watch my first video from you on gas chromatography and it was easy and fun to learn. Thank you.
Thank you so much 😭😭😭 its really easy to understand your explanation
You're welcome. Glad to hear that you're getting benefit from my lectures
Very nice presentation .....understand everything in simple path........Thanks alot.....wish you a nice future,,,
Thank you so much, Suman & Shomu's Biology, very well explained.
I have an examination tomorrow...thank you ❤❤❤
You're welcome
now i undertand this ! thank you so much
By the grace of Allah, by seeing ur vdo i got job in reputed pharmaceutical in bd, thanks
Thank you
Very good explanation about hplc thank you sir
this was really helpful thanks so much for the video
but my teacher is making us study a diagram with a pulse damper, drain valve, filter, back pressure and transducer and i've no idea what those are for lol...
Very clear explanation! As i'm using this technique for my research this was very helpful.
+Sander Zwaan thank you. Glad you liked my lectures
Shomu's Biology it's very clear to explain.thanks sir
Man you`re soooooo good;your english better than other Indians
Thank you for appreciating my efforts
best explanation on HPLC.. 👍
Thank you. Glad you liked my lectures
Best teacher in the 🌎
Thank you so much for appreciating my efforts
Thanks a lot Sir.. Highly Helpful for my Sem Exam🥺🥺🤗🤗
You're welcome
Way of explained..just amazing. nd .understandable,❣️
Thank you so much for appreciating my efforts
Good explanation, it is in general, if yiu explain with a protein sample, it is very good to understand what chemicals we can use
Very well explained, I came here to refresh my memory but thank you sir!
Glad to hear that you are getting benefit from the videos
Thanku for your explaination
This is the Really great video about basic idea about hplc working technique , and i request. To u sir please upload a video about hplc operation eg-how we can start and whats is the parameters of a hplc test like -system suit, washing, condition column, purge injection, dry prime, wet prime, tailling factor, breacketing, etc
I think these factors may vary between different HPLC machines
Thank you, you explained this so well!
Thank you so much for appreciating my efforts
Great refresher, thank you!
Waoh its straight and the explaination is so clear. thank you
very nice presentation, full logic concept on the hplc. thank you
+Yarro Paschal thank you. Glad it helped
wonderful explanation brother u done a great video is very useful for pharma students....
Once you get past the accent. The video provides alot of information, which is explained and structured nicely.
Thank you for doing what you do. It makes a big difference in the lives of students. ❤️
Thank you sir .Your videos are very helpful
You're welcome
Outstanding explaination. Thanks a lot.
You're welcome
Thank u so much sir for this video, my lecturer stutterers and it's difficult for me to understand him because he's too fast
Thank you. Glad it helped
Thank you so much, sir! It helped me to have insights about HPLC for my (which is supposed to be our) thesis :)
You are an awesome teacher.
Thank you. Glad you liked my lectures
Plz discuss about granulation.... Thanks for your discussion with us..
You are really good! Easy to understand!
Thank you so much for appreciating my efforts
briefly, nicely explained #HPLC
good speaker thank you sir.....
thank you so much ! this really helped.
+Nour Ghazal glad it helped