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How to Grow ANY Plant from Seed
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- Опубліковано 7 жов 2023
- Please enjoy this synthetic seeds (aka biological boba) tutorial with a side helping of covid. This tutorial is definitely more intermediate-level but I have some beginner still air box videos coming out very soon so make sure to subscribe! To clear up some confusion in the comments: this is not a replacement for real seeds! This is a method to preserve plant genetics in a small space.
NEW MERCH: bit.ly/PIJ-shirt
Check out the video that Francisco and I filmed on Plant Cell Technology's channel!: • I Had A Great Chat Wit...
/ plantsinjars_tc
Want to try tissue culture at home? Use code PLANTSINJARS for 10% off your Plant Cell Technology order: www.plantcelltechnology.com/?...
Supplies to Make Synthetic Seeds:
• Murashige & Skoog: bit.ly/3pMpAOr
• Sodium Alginate: amzn.to/3rB42pA
• Calcium Chloride: amzn.to/3Q1aMX8
• Micro-pipette: amzn.to/3Q4gkAf
• Pipette tips: amzn.to/3rAaPQh
Timestamps:
0:35 Preparing Solution A & B
3:29 Isolating the node
7:32 Making Seeds at Home
Please note that some of the links in the description are affiliate links, which means that if you click on one of the product links and make a purchase, I may receive a commission. This commission comes at no additional cost to you.
I have everything to do tissue culture. I've been wanting to get into doing it for some time now. Keeping mothers and clones of my favorite plant is a bit of work by its self. Thank you for the video.
Somewhere I was looking, they wanted 200 for the class but 2,000 for the cannabis class! Just like everything else that has for Mary Jane in the title, that price multiplies exponentially. Plants are plants, some easier than others, since some are woody, which would seem difficult, maybe not difficult but different
I am going to DC in 2024 to do a class with Francisco. I have been growing fungi since the mid-90s, so I have 80% of the materials that I need for micropropagation, but I think the class will be fun, not to mention educational. Plus, I want to meet others that are into this hobby. IMO, these techniques and the technicians who practice them will be in HIGH DEMAND as we reach out into the future.
The class is really fun and you'll learn a lot. I actually went to the most recent one :)
You sound like Bill Gates.
That is not a good thing.
See you soon 🎉
What fungi?! I'm interested in growing eatable wild mushrooms. They do however need their respective symbiosis with the tree/soil..
but why is he making such a point of the components being sterile while working in open air, and using a paper towel as a workspace?
This is what research is for. Trial and error will occur during these periods of research. This was informative thank you.
Ive been looking for a video of this process for years. Thank you Laur and Frankie!
Doing the lords work with these tutorials, packed full of detail and great info
Your videos are great!
Very informative and thank you for making them!
It is something that I have always been interested in. You are a big help for me to learn more about what it is and what it takes to tissue culture plants.
You’re the best thing that’s happened to horticulture since the concept of botanical gardens
Wow, this is so awesome. It shows me how delicate yet tough life is.
Love your vids they are the best! The edits like the news on are amazing. Thx for the joy lol
holy crap I've been looking for something like this for ages thank you so much!
Very Very cool! I had no idea this even existed until you brought this to us.
i've just discovered your channel today and woah- so cool. thanks for sharing all of this !!
That's fantastic, I'm learning so much with your channel
I love the manicure glass bead sterilizer you use! I recognized the brand and it honestly is exciting to know that the product works well enough for lab use!
Francisco is great. Seeing his lab was neat, especially the scope with the digital camera. Neat video. I liked that I even knew how to do a small part of it; the mixing is just like making gravy. One trick with gravy is first make a slurry by putting the powder in a little bit of liquid, and then put that into the rest.
Holy shit. This channel is a fucking gem.
Really amazing process
I am going to have to try this over the winter.
20% germination isn't that bad. If you compare it to the percentage of real seeds that actually grow (in the wild) it's very good rate lol
Well, in relation to the effort put into these synthetic seeds, 20% is extremely bad compared to directly planting the plants fresh from tissue culture. Why would you make these; just to better store them? You can simply keep some fully grown plants alive, from which you can take samples at any time.
These would be cool, if germination-rate could be drastically increased and the seeds would actually allow to plant huge fields from one donor plant. This only applies to indoor farming, since these mono-cultures would be highly susceptible to pests due to their lack of genetic diversity (see banana fungi problem).
@@haifutter4166 "Why would we make these?" yea for normal alone person it doesn't make sense. If people or companies are trying to spread their clones to others via sale or free etc then it makes sense. Eventually it'll get automated so the 20% isn't so bad in that regard... by that time we'll probably see an increase in the rate too
20% is pathetic.
@@Aussiehomestead1965 some seeds have like 3% naturally
i propagated all my plants using cuttings in an aeroponic cloner and allready felt like a scientist :D seems like i found a new method to try on my thyme and rosemary. very interesting!
For anyone wondering the alginate and calcium chloride technique used is how they make popping boba in boba tea (bubble tea) 🍵
It reminded me of some of the "molecular gastronomy" techniques.
Yes! But with MS media 🎉
It's all recipes and cooking and cleaning glassware, etc..@@PlantCellTechnology
For anyone who don't fact check what they read on internet...
Bubble tea use tapioca, not alginate.
Alginate + CaCl2 is use on molecular fine cuisine for 'caviar/pearl' of different flavour.
Wow I'm impressed
This is really interesting. Thanks for sharing!
So whose thinking of making a fortune with artificial salvia seeds by mail delivery?
Nobody. Buy shroom spores instead. They are legal for scientific purposes of course
@@ItemN9ne they require sterile conditions and do not produce salvinorin
I want one
For those of us on a budget, you can use a clear disposable straw in place of a micro pipette.
FASCINATED!!!!!!! Thanks
Cool, if these seeds had a better germination rate I could see this being a great way to cut down on some of the tissue culture in lab since a lot of what we have is just propagating old plates so we don’t loose the lines.
I loved that face after he said the blade can really cut you up 😂
Thanks so much for uploading a new video. I was withdrawing from your content. But now I get my fix 😂. Synthetic seeds! Omg brain overload. So much to learn
Great video! I would like to try this with auto flowering cannabis, since they are not clone friendly. Would be a game changer. 🍀
You could put the jar in a pot of boiling water rather than the microwave as you can keep stirring.
"be the hot plate, you are the hot plate" hahaha amazing 😂
Channel your anger, and be your own hotplate. That is what I say.
AmScope has a variety of quality microscopes for reasonable prices. I have both a light and dissecting microscope from them and they are comparable in quality to the ones I use at work. I 3D printed a phone mount so I can take photos directly through the eye piece.
You have a bright future in science!
When i grow up I want to be a hot plate.
😂
Good protection for your seeds. Thanks for your video.
👌💎💎💎💎💎
what a nice lifhack, exactly what i need for my project, thanks
WTF.... I'm learning so much from your channel. I never even heard of this.
I just saw a video that made me think of you and your efforts. A man came across surviving American chestnut trees that are seemingly unaffected by blight. First I wondered if these trees may have some natural resistance to blight. Second I thought perhaps they could be propagated via tissue culture. I know efforts are being done to alter the genetics for disease resistance. But, it seems to me propagating trees with natural resistance would be the way to go.
It would help in a sense, but they would be clones of the same genetics (or the diversity would be greatly reduced at least) so the trees propagated could wind up vulnerable to other threats on a very broad scale.
When you spread genetics far and wide, if they are all the same or very similar you will often find they’re all vulnerable to the same diseases and they spread it readily between each other.
I saw that video as well.
have to try it
That s interesting philodendron
I know it would take a long time to make each video, but have you ever done a "growing a ___ from start to finish using tissue culture"? I'd love to see that.
I'm working on a few videos like that! They just take forever to make
good info, i just such the information in, hoping to do my own experiments, hopefully economical as well .. cheers :)
Awesome!! Thanks
This is fn awesome.
interesting use of molecular gastronomy techniques.
very interesting and surprisingly simple technique. When he pulled out the Alginate i already had some idea what he's going to do but it was very useful for me to see precisely what plant part he is using for the seed. I think i might do that at home as well, thank you very much for the informations!
For storage of the "seeds" just a cold environment needed? no light requirements? then looks like start growing it like any other normal seed?, very interesting. perhaps a good way to ship larger numbers of plants reducing possible damage during transport as well as size of package. Have you considered cococoir for a growing medium? the compressed bricks they come in can be rehydrated and autoclaved (at same time even I would think) to be sterile. I found cococoir to be the best substrate for rooting cutting and germinating seeds when playing around with hydroponics. Coco just seems to have perfect environment roots love and a neutral PH7. I used to just rehydrate the coco bricks with boiling water to steralize before using. Thanks for this, I had no idea this existed haha.
This is really cool. I can't figure out why you would do something like this (other than for fun and the cool factor). Seems like you could just plant the plant lol.
Maybe for additional ways to clone very rare expensive plants, for one
@@my2wins yeah i guess i just dont see the value really. The plant is already cloning itself. You just incase it in an sphere. Then "plant" that sphere. That is like taking a normal seed and instead of planting it you wrap in it another sphere and plant that. It doesn't really offer any benefits that I can see.
Oh, edible wild mushrooms were something to multiply and grow by creating "seeds" out of them. However, they need the symbiosis with their respective tree/soil is essential! Interesting 🙂👍
Glad to see youre doing well! In April I cut my hand like you did 😂
Sooo good ☺️
o.k. o.k. that fits into my actual insight on propagation by cuttings.
its the same, but on smaller scale, right?! does i work with any plant?
Hey! Your videos are amazing. Just a technical question on your videos, what camera/software do you use? Your background/green screen set up look really great! I've had problems in the past with background/greenscreens for my work from home set up, so I was just curious.
Thank you again for the amazing content!
Hello, I love your videos. I have a question on the microscope. It looks like Francisco has one of the amscope binocular stereo microscope. Do you know what magnification would be suitable for tc work? I'm looking at one that has 10x and 20x magnification. Thanks!
Goals! Saving up and searching out equipment now! Thank you!!! 💚💚💚
HONEY WAKE UP PLANTS IN JARS POSTED
Did you have good luck growing them? Do they take longer than regular seeds to grow? Can they be contaminated, or would they have died before getting to this point?
Andonstar microscopes for electronics are super cheap and would probably work pretty awesome for that
I´m surprised, how easy that is. It seem´s to be a method for everyone?! [ ... ]
Green screens and "Philodendrons" ;) Gettin' fancy!
Make a video about how to get rid from viruses using tissue culture. There is a bit of info on the internet about it, but it is not clear enough
Once I get a microscope I definitely will try :)
I'm wondering if I could place some mycelium in stasis with this.
Not sure how I got here unless YT related it to gardening, but now I'm intrigued. What is the purpose of this? And why is it used? Thanks!
Now you have to try tissue culture, that's how this works
very cool! the "biological boba" cracked me up lmao. when i eventually get some equipment, I wanna try this!
Wait what? I had no idea this existed. That's crazy
Beautiful charismatic and smart young lady doing science!
We don't have enough of you, keep on rocking babe!!!! 🎸
Thumbs up from Montréal
I wonder if it can be done with potassium algenate with greater success.
I could see tissue culture being really useful for genetically modifying plants since you can start with a single cell and create an entire plant from it.
This is indeed an essential part of the process in nearly all species.
@@greggoralogia7401 I mean as opposed to modifying a germ cell or a series of cells in cluster after gamete fusion. Obviously all life starts with a single cell haha.
You could get a mature plant capable of bearing fruits or clones from a single cell with no seeds necessary.
Definetly, genetic engineering in plants is almost never in the germline though, pretty much only Arabidopsis and a few brassica species are amenable to that technique, everything else requires tissue culture. I would love it if it worked during fertilization in a wider array of species! It would make my job so much easier.@@Nanamowa
Nice video
Serious question. would giberrellic acid help the success rate? is it used in tissue culture?
Living orbees! This is gonna sound weird but I kinda wanna take a bath in them lol.
How many jeers are they good in the breezer?
I would subscribe, but I'm worried I'd become too obsessed with this channel, I'll just go now.
How about Calamus sp. LOL? Something a little more difficult than Monstera's etc.(that grow like weeds anyway). Cool channel. I'm a biologist Tissue culture, way to go! I've subbed too.
Does this procedure need to be done from the tissue cultured plants or can this be done from sterilized plant material from a mother plant?
The laugh @ be careful with the blades
So what's the use case? What would you do with these that couldn't be done with a cutting, with the germination rates being so low and the process so tedious? Just why? x)
I'm not complaining, this was interesting, I'm just confused.
I like real seeds Most plants can do fine on their own. That’s why I planted 20 million plants and trees this year.
I am a student who interested in genetic modification in floriculture. I need to know about , is it possible to do color pigment of flowers change in every plants?
Wow so interesting! So can you actually make a female cannabis seed this way?
I'm not shure what to think on one hand very cool never even heard of synthetic seeds on the other hand I'm very close to the earth. People seem to be cool people.
You should make a video on how these seeds can be used to grow out a plant. Maybe title the video: "Lessons for luddites"
Can these be shipped usig an ice pack?
Could these survive shipping?
What are you adding at 1:33?
Molecular Gastronomy technique is called “Reverse Spherification”
I don’t do tissue culture and find your vlogs so entertaining. Great job at production, filming and editing. Thanks.
thank you!
I have a question for you. Do you know if the chemicals involved stay in the plant and can cause long term damage?? I would like to try this on vegetable/fruit plants, but I'm concerned if those chemicals involved stay in the plant, and even into the fruit??? Or do they dissipate after some time. I would assume the plant actually makes use of the stuff, and then grows healthy enough that the chemicals don't exist in it.... but just curious. I appreciate any response.
Apparently, you need a well equipped lab....
Finally a honest realistic and fun channel about plants. I want to learn so bad.
Cute
Great Video!
(The sodium alginate Link in the discription is the wrong one 🙂)
OOPS thank you
Could this be done with bananas?
Put a seed inside of a saturated water saver Crystal 🌱
This is a new epoch in plant propagation. It's now coupled to human industrial processes.
What is "ms media" at the beginning?
“LETS GET INTO IT” 🙄
You forgot to move to cover the camera lens in your transition with your hand like ALL THE OTHER KOOL UA-camRS do. 😂
Do you have to use a pressure cooker to autoclave things or can you just use a steamer?
I don't really know the answer. Although a pressure cooker at 15 psi reaches 120c where a steamer would be 100c or less. So I assume the higher temperature would kill a broader range of microbes.
its like making popping bobba