DIY Tissue Culture: How to Get Started for Less Than $200
Вставка
- Опубліковано 27 вер 2024
- I sacrificed a finger to bring you this video. please subscribe
NEW MERCH: bit.ly/PIJ-shirt
Join the Plants in Jars discord server! / discord
Where I buy tissue culture supplies: www.plantcellt...
(use code PLANTSINJARS for 10% off)
/ plantsinjars_tc
Supplies List Below!
• Scale (0.01g accuracy): amzn.to/3I9fDRM
• pH Meter (0.01 pH accuracy): amzn.to/41CqJFA
• pH Up & Down: amzn.to/3pAM19r
• Spray Bottle: amzn.to/3pI3Q6g
• Plastic Gloves: amzn.to/41CBBmW
• Forceps: amzn.to/42K3VFa
• Scissors: amzn.to/3I9dwgK
• Sterile Petri Dishes: amzn.to/3nPNHLT
• mL Droppers: amzn.to/44X8cXl
• Agar: bit.ly/3o4yPcu
• Murashige & Skoog: bit.ly/3pMpAOr
• Kinetin: bit.ly/41IpXaa
• Polypropylene Containers: bit.ly/3pJrlMF
• Light Meter: amzn.to/42B1THI
Protocol for video: www.researchga...
(I am aware that Philodendron Mamei is not technically a "self heading philodendron", but this protocol has worked well for me before for other similar philos!)
Timestamps:
• 1:31 Building a still air box
• 3:18 Tissue culture supplies
• 4:45 What is a tissue culture protocol?
• 5:03 Where to find tissue culture protocols
• 5:44 What is tissue culture media?
• 8:55 How to read a tissue culture protocol
• 11:00 Making tissue culture media
• 13:52 Sterilizing the media
• 17:19 Prepping the still air box
• 18:30 Preparing the explants (tissue samples)
• 19:39 Putting the explant into tissue culture
• 25:30 Conditions for growth
• 26:19 When to subculture
Please note that some of the links in the description are affiliate links, which means that if you click on one of the product links and make a purchase, I may receive a commission. This commission comes at no additional cost to you.
I am a biotechnology engineer and it is wonderful to see this kind of micropropagation content! Let's hope that more people get their attention to this beautiful thing about science!
I had never heard of this until I came across this video. Now I’m freaking hooked
Hello, fellow BioTech! Yes, I agree with you 100%, now I would add that a good source of information is a book titled, Plants From Test Tubes, which adds a little more science to the above video. PiJ, thank you for the amazing content.
I got an associates degree in horticulture, but couldn't justify travelling to the land grant school for a Bachelor's, so as a certified plant nerd, this is the raddest thing ever. I remember buying a pineapple plant propagated this way from Disney's The Land ride, it made me want to study plants and hydroponics, and I love combining tech and nature. Keep this up, please, and inspire the next generation of plant nerds.
It was actually great having your fiancee do it live, with your coaching and notes! I feel like you added a lot of really helpful details that someone with experience might otherwise forget.
As a biologist, I find your work very-very impressive, the content you share here is amazing, I am stunned! And of course I've just subscribed to your channel :)
I do this professionally in a research lab. I’m honestly impressed by your work. Great job and keep it going!
I really appreciate that! Thank you
Great clear and easy to understand Video😊
Can you answer a question that was popping up in my mind, when watching this? The process doesn't look too different from regular plant propagation with cuttings. Wouldn't this techniqually also work without the hormones? My guess is, that the hormones just speed up the process?
@Izzy-fr1zu Was thinking the same, it was curiosity for difficult to strike plants that led me here
@@Izzy-fr1zu Yes, there are a lot of plants you can tissue culture without plant growth regulators. PGR’s can speed up multiplication and rooting, but sometimes (if used in too high of quantities) they can stunt plant growth
He's trying so hard and its so hekkin cute!! The way you can see every single half error he did (note: yes it was his first time said half errors are borderline expected....) But he was like wait wait fix fix... So wholesome
Thank you for helping make tissue culture accessible!
Your channel is the best on TC!
👍
@@nee3029 Thanks for your support! And, you like our content.
I am 60-year's old, and a Life-long plant Lover.....when I was around 13, I went into a Woolworth's that had a luncheon area/kitchen/diner. I found a Product Stand with Test-tubes containing clear gel and a small portion of plant growing in it. The Cardboard backing it was attached to advertised colorfully as "Grow anything in this medium", and I was captivated. To me it seemed like incredible Science to do such a thing, ...Cloning! I really wish I bought it, but knowing Regulations in those days, it was probably best I didn't considering the Ingredients you are talking about! :) I might of grown a few extra fingers, and then some! LOL
I have worked many years in many labs doing this for strawberry and other species. The fact contamination is not a major issue for you is a miracle since for many people, working with wild plants, contamination is always a problem. Great job
its all about sterile technique. If guys can be really efficient with home mycology with home made contraptions, this shouldnt be an issue at all
Its all relative. I did mammal tissue culture. After that most other cell or tissue culture seems easy, even cell culture of Hela cells or other mammalian carcinogenic cell lines is much easier than working with wildtype cells.
Let's go! Great video series. In my plant pathology grad student years, I did tissue culturing to remove viruses from fruits and vegetables in a lab with probably $50k+ gear. Getting this down to $200 to bring the science to the masses is just awesome!
I even added gloves to my still air box when I was working with liquid culture making Petri dishes growing mushrooms
After watching other tc channels, and reading plants from test tubes. I like yours the best. In my opinion they do things that seem odd in process. 🤷🏻
Omgosh!! I am so excited your video showed up in my feed. I love plants and own about 140 houseplants. I love studying botany & science. You really broke this down into such an easy to follow tutorial. Loved this! I have always wanted to do this with orchids. I'd probably also do this with all my Orchids, Hoya, Dischidia, some ferns, Jungle cactus, Zig Zag Cactus and my philodendrons. Thank you for sharing and I am honestly so sorry you got injured badly. A 3 day hospital & severed tendon are a huge deal. I hope you don't have nerve damage & your rehab goes well. So thank you for your sacrifice for Science. I can't wait to binge your other videos abd my inner nerd is super stoked! Take care & get well soon.🫶💚🪴☘️🌵🌿🌱🍃
Thank you! I have a video on my channel about cloning orchids from stem propagations if that helps! It's an older vid so the quality isn't as good but the info still holds up. I am also an orchid lover :) Someone gave me a great idea this morning to make a video about growing orchid seeds in vitro.
You can also use baking soda to up the PH and distilled white vinegar to lower it to save some extra bucks 😊 I love your videos! Thank you for sharing thorough easy to follow info on PTC✌🏽🌱✨
I love this tip! I wish I had mentioned it in the video
@@plantsinjars baking soda adds alkalinity but not pH. You could use a diluted NaOH (lye) or liquid calcium hydroxide. You did an excellent job on this video! I am looking at growing giant bamboo here in the Philippines commercially, and your video is the best explainer I have seen yet. Thank you!
Vinegar is a very poor choice it has chemical similarity to the auxins. Normally phosphoric, sulfuric, or nitric acid would be good choices in hydroponics as they are all plant nutrients, citric acid may also be good and does not effect the nutrient balance(food grade is often found with canning supplies at low cost). Phos is the most common hydroponic pH down.
Non-adultrerated ammonia is a fair choice for pH up (it will convert to more stable ammonium as it grabs protons) but KOH or potassium carbonate are also good (not bicarb. aka hydrogen carbonate). Most other bases are either poorly soluble or will interfere with the biochemistry; sodium is generally tolerable in very small amounts though a few species are quite sodium tolerant.
Nitric acid is a tricky one for normal hydroponics with large active plant growth, plants love to absorb both -NO3 anions and +NH4 cations in much larger quantities than other nutriants. The plants exchange -OH ions for nutrient anios which raises the pH and with nitric you can end up chasing the pH if not aware of the bigger picture. Likewise ammonium cations +NH4 get exchanged for +protons and this lowers the pH. So the two must be balanced, and that balance varies with plant specie. The situation is slightly different with tissue culture because of limited nutrient uptake, and the media is a gel so the bulk solution cannot readilly buffer local changes in pH.
@@TheDuckofDoom. wow, lots of info! But the whole reason I mentioned vinegar was the very affordable cost lmao because that was the point of the video? Yes, if you have a bigger budget and access, of course get the best of the best. All I know is vinegar is like 3 bucks for a gallon 😂 and my cultures have turned out great if I even had to use vinegar. As you know, before adjusting PH, it tends to be in the low 4 range. Vinegar isn’t needed unless someone went too far over, which is less likely. Btw, back on topic of budget, how much do the options you mentioned cost?
@@nolongerabandoned it literally changes the PH. Have you tried it? The meter don’t lie 😂 But baking soda is a great option for someone starting out on a budget. And if you do need to use it, you use a pinch of it. It doesn’t take much at all to raise the PH.
This popped up in my recommended feed, so glad it did. This content is truly amazing, and I'm shocked you haven't hit 100k let alone 10k subs yet. Keep up the great work, truly amazing and educational!
Greetings from Srilanka. Very practical and informative.
" The couple that works together , stays together."
Nodes are generally the best plant material because they contain an accumulation of meristematic cells ~ the 'building blocks!'. I'm an arborist but I grow everything including mushroom and fern spore cultures. I just started tissue culturing birds nest fern prothalli because I need them to grow and multiple much faster. This was helpful thank you.
This is actually great, when I went through my biochem major, part of our senior project was industrial produce research & production then up scaling the process. We even did plasmid insertions for specific trait expressions. It was good stuff, we got to work with the Chemical Engineering class in teams to scale the process. I like things like this, it’s good to see younger people on UA-cam doing productive things.
One thing that struck me here is the quality of the comments... says a lot about the quality of her content!
I know y are too young to have seen a movie called "Green Fingers" But when I saw your arm and of course the subject matter of this video; I thought about that movie. Good stuff, I used to do a lot of micro for work and one particular company refused to buy a laminar flow because they6 said it was too expensive, so I was forced to do all the culturing in a small closed room just using basic aseptic techniques. Despite losing many cultures (fungi) to other non specific ones, the usual culprits, we did indeed succeed with "jam jars" and no "proper equipment" LOL.
Really nice explanation. BTW, scalpel blades can be inserted manually, you just have to know the technique. During surgery, we place blades manually but remove used blades with a tool. Only to avoid slicing ourselves with a used blade.
I’m pleasantly Amazed!! 🤯 I am a rookie mycologist and had no idea this was a thing! She is Great, Funny and Realistic! FYI, people can get a still air box made from plastic on Amazon, they can sometimes be cheaper, also they do have disposable surgical blades that do not need to be changed. thank you for the introduction to this New hobby and look forward to all your videos!!🫶🏼
Great video! I've cloned plants, and always thought Tissue Culture was only possible through highly trained scientists in a sealed lab..?!
You sure busted that myth, lol! IDT anyone could have broken it down into a more simple, understandable method. A new channel to subscribe to!
Many thanks, and much respect!👍💯✌️🙏
Thank you!
found you because of youtube algorithm, i didnt even know tissue culture was a thing 20 minutes ago and here i am buying all this stuff off Amazon lol
the algorithm knows me pretty well!
Tell me how you did! Before I buy it all😊
Please. Did it work
I can't believe how much this taught me, great content and super informative! Thank you so much.
I am really impressed by the clarity of your video. And I look forward to see a follow up video how you separate those shoots and root them. Thank you!
thank you so much for this video! it was the best video i have seen so far on getting started in tissue culture. i appreciate you considering budget, depth of detail, showing the procedures, and for making it seem so approachable!
this was a hidden gem for me. dont regret clicking on this video
Not nearly confident enough to take this on yet, but subbed from two accounts because you sacrificed your tendon.
You are more ready than you think! Let us know how it goes if you try it
wow love your channel, I am graduated in medicinal plant biotechnology have being working with tissue culture since 2010, love your channel, and you are very cute my gosh
This is amazing, I have been doing amateur mycology for some 2 years, had no idea you could do this with plants. Thank you for sharing your science.
? this has been done in the aquarium plants hobby for such a long time way before house plants
Amazing video ❤ I worked with tissue culture when I was in University at Mexico and we did not have enough money to buy proper equipment so we sterilized the material in a pressure cooker too and used baby food jars 😊 and they worked really well
I found you today and I am happier for it. I have been growing mushrooms for 25 years and have always wanted to tissue culture. I have lab grade equipment thanks to my fungi obsession so I will only need to buy the media. I am ordering supplies from your links and getting started. Thanks for prodding me into action...PLUR
We use UVC bulbs to STERILIZE our growrooms
5min intervals x 3 times per day
LITERALLY KILLS ANYTHING ON CONTACT - Spores, bacteria, Insects.
We use it as a PREVENTION TECHNIQUE
NO CHEMICALS
Make sure you're not in the room when the lights come on because it's highly cancerous to human skin but the plants love it and you can accidentally kill your mushrooms or your good bacteria with it if you're not careful
This video just answered so many questions I had on tissue culture from just not looking into it. I always thought tissue culture was from scraping any area of a plant so you'd have a bundle of cells and from there a plant would grow in the medium. Makes sense that plants would be propagated from the node though. Maybe one day I'll be able to put this video to good use. Thank you!
Congrats on the views for this video! Your passion and hard work is very inspiring . Keep up the great content.
Well well well... Looks like I have a fun planty project to do over winter break 😁
Hi, Great vid. Your clean box is very similar to the one i use for my brewing yeast culture play.. However i use a pane of glass on the top of my box instead of inverting it onto its lid, which provides much better vision, and also helps stop inadvertently breathing into the box when peering in through the arm holes tho that could be just me..
This is fantastic. I was literally commenting on people spreading misinformation (or just asking about) on succulent propagation from "cuttings" and i said it wouldnt work by cutting the leaf, except in a laboratory setting, talking about tissue culturing. 😂
I never considered this was something that could be done without lab equipment so i never even looked into it. Super cool, itll probably be a year or 2 before i ever try this, but ive built a couple flow hoods for mushrooms, so when i move into the new house eventually i think a flow hood will be one of my first projects. This will be second
❤❤❤
I'm so happy you finally made this video! It helped me a lot! 🤓🙏
Thank you! I'm glad it was helpful!
As far as first plants to tissue culture strawberries because they are easier-ish and there are a few rarer varieties that I'd like to incorporate in breeding Premier aka Howard 17, and Catskill.
Next would be peach, to preserve rarer types like Triogem.
I’ve been wanting to try strawberries! Let us know how it goes
Ok wow, very comprehensive! Love the tutorial ❤
I hope you recover quickly from your injury 😢
Thanks for this! I have been reading a lot of books about getting started, and the visual representation is really helpful. Sorry to hear about your injury. Best of luck on healing.
Thank you! I’m glad you found it helpful
Techplant brought me here! As a biology student I find this to be very interesting and I might just try TC myself. Thanks! :)
Let me know how it goes if you try it! Techplant is awesome
A really great resource. I do however urge caution with regards to any 'math' done by LLM AI such as chatGPT... it is very prone to error, so just be careful, and for important calculations, double check the calculations (at least in 2023)... BTW, awesome info about the recycling symbol.
Just discovered this channel [subscribed]!
Sorry to hear about your fingers!! This is a fantastic video!
What a great basics video! So sorry to hear about your finger. I'd like to try some chimera African violets since they can't be grown from leaves. I'm also interested in meristem culture to clear violets of INSV but that's a bit advanced for now.
Thank you! Clearing INSV through culturing meristem tc sounds extremely interested. Keep us updated on your progress!
OMG, you're such a very good teacher! Thank you so much, I learned a lot! GBU! 👍🏻🏆💝
🔬🌱👏 Your professional work in a research lab is truly impressive! It's amazing to see your dedication and the results you've achieved. Keep up the great job and continue pushing the boundaries of your field. 🌟💪 Your passion and expertise are inspiring, and I look forward to seeing more of your groundbreaking work. 🚀🔬 Keep the innovation flowing!
as a veterinary that worked with RSV culture and a aquarist that love planted tanks... think that your work is AWESOME!! would love to hear from you something about "aquatic" plants... echinodorus, cryptocorynes, aponogeton, etc! greetings from japan/brazil!
:O ROBS IN THE VID!?!
Fantastic and very impressive Ms Laur. Thank you so much for the great video. lots of work and time needed to do editing like that etc. Hope your finger has recovered. God bless you.
I love this! Don’t think I will be doing tissue culture but I love watching the science behind it! Thank you so much, both!
tip on light meter; most phones have one for auto brightness. If you have a grow light with PPFD maps you can calibrate them and they are ususlly a good reference.
As far as ph goes, the strips/ph indicators tend to be more accurate than the cheap meters.
There are possibly reasons not to do this in these circumstances but a flame can be useful in sterilizing tools too, just becareful near alcohol obviously.
Speaking of, a spray bottle of isopropyl is good for sterilizing equipment.
Well presented video, makes it seem less intimidating overall👌
Actually planning to build a laminar flow hood, just will take a while to acquire all the fitting parts
That is awesome! Keep us updated on how it goes
Gordotek has a great video on laminar flow hoods 👍 I build a similar one and it works great
ua-cam.com/video/lInfdAVvBts/v-deo.html
I can second the GordoTek laminar hood easy to build and it’s working for my mycology. I also use a instant pot instead of a pressure cooker. thanks for starting me on a new hobby never thought of home tissue cultures. Thanks great video. ❤
Never seen mycology methodology used with plants. Good to know!
Ok, I'll stop using my bare hands to set up my scalpels.....
I was into Mycology for nearly a decade. This is very similar in so many ways and will be easy to cross over. Thanks for making it easy to understand
Awesome video! You are really getting better at editing and creating with every video!
Since I've done tissue culture many moons ago in a lab, things appear to have gotten a bit easier (we actually had to make our own MS Medium from scratch with the individual chemicals!).
Now I'm working to get back into it in my home 'lab'. I have quite a few of the supplies, but I'm still not satisfied with any of the culture containers I can find. I do however... *really* like (dare I say ❤) those jars with the PP lids you are using. Can you please share where to get those?
Sure! They are the 240 mL culture vessels from Phytotech. Thanks for your comment! phytotechlab.com/culture-vessel-glass-70-mm-8-oz-240-ml.html
@@plantsinjars Awesome! Thank you so much! And they have them with filter vent tops!
Hi Laur,
Thanks a lot for sharing.
I tried several times but each time the cuttings got contaminated.
You don't get problem when you leave your tools in bleach?
After my TC, I wash all tools with water and wipe them but all my tools get corroded each time I use and sterilize in bleach.
Working with anthuriums these days.
Daniel Jheelan from France
@@danieljheelan5256 You may want to try sterilizing everything (both the tools and your tissue culture media) in a pressure cooker or insapot before doing the transfers if you're having issues with contamination. I usually pressure cook everything at 15 PSI for 15-20 minutes. I have a few videos about this subject on my channel :)
You are my newest favorite smart person!! Thank you for sharing this knowledge and compiling the list of resources. I hope you are healing well, and I look forward to joining your youtube community. Please don't beat yourself up emotionally about the accident. We all have lapses in judgement.
i have tissue culture stuff for less than 30$. Not to mention propagation via water/vermiculite or perlite, which is like 7$ at most
I’m sure it was somewhat frustrating directing a newbie to do the transfer process but I think I got a lot out of learning from the mistakes. Thanks for the video!
Hello, thank you for this video. I have toyed with the idea of doing this at home, mostly out of curiosity.
This video is convincing me that i don't have the time a the moment...
I don’t expect I will actually do this, but I have been quite curious. Thank you for the concise but wide ranging tutorial! My son cut the tendon in his left thumb cutting a zip tie off a coil of rope, while camping a two hour drive from home, and just about as far from a hospital. It all worked out, he had an excellent surgeon, and four years later it is just a memory.
Fun fact that symbol on your plastic is not a recycle symbol. It's a plastic classification symbol. This was a ploy by big plastic they made a symbol similar to the recycling symbol to trick ignorant customers into thinking that all plastic is recyclable. While more than just numbers 1 and 2 are recyclable, 1 and 2 are almost always the only ones recycled because the cost of recycling the other numbers is way more expensive.
If you couldnt tell by your views on this vid... TC is HUGE in the cannabis culture and industry. Not a whole lot of folks offering this info for free or willing to take the time to show. But anyways... Im no pro but i knew more than the basics on TC... I REALLY appreciate the subject, content and your channel. You now have a new loyal follower
I am now totally invested in tissue culture. Please stand by while I go purchase all the necessary items and lose myself down a rabbit hole that will rival the one I fell down after I thought, “I wonder how hard it is to use a spinning wheel?”
This was fascinating! Thanks for showing that the barrier to entry is doable at home to this super cool technology
I can’t wait until you get to a million subscribers and you will!!!!
"moo-rah-shee-geh" EDIT: The still air box is a really good idea and inexpensive to construct. Although, if you have good technique it isn't strictly necessary. I took a cell culturing class where we sterilized and implanted tobacco leaf, African violet leaf, carrot segments, and corn embryos on non-antibiotic containing MS media just on the bench. The rate of contamination was higher than under a flow hood, of course.
UNDER 200 dollars!?!?!?
That's a good price especially when you look at how expensive it can be without a great guide like this!
Monsteras (decent ones) start @ $50
Can be cheaper if you already grow mushrooms
@@lazy5373 considering a mushroom is more closely related to a newborn baby than it is to a plant, i struggle to see the relevance of this comparison. Obviously the needs of all three organisms are radically different
im going to do this with my cannabis cultivars to keep them long term storage. I will share you video with the cannabis community.
pls also make videos on culturing aquatic plants used in aquariums
Lol i didn't even realize right away as you were talking with your hands that you had a rap. Really glad you made this video. The fact that you include relivent useful information is so nice thanks. Hope your arm heals up ok
I cannnot wait to try this. I got a Blue Basil Culture from Disney and I’ve cloned many plants but wanted to get into tissue culture. I couldn’t find any videos about this at all a year ago. I’m so happy now 😂
Plan to tissue culture the plants I like to keep. Cannabis, and thankyou for the video, the info on the media was essential.
I’m SUPER new and found out about tissue culture because I wanted to sell houseplants online. This video made it so much more approachable!!
Thank you, your attention to detail & simplified short cuts are truly inspiring along with your enthusiasm in doing something you enjoy,
I grow a huge amount of perennial & annual garden plants & this year I bought in some tissue cultured delphinium & my knowledge in this is..& was limited but truly find your explanation a joy & considering setting up some basic laboratory myself. I currently have a ever growing collection let alone the ones I sell, it's such fun & I have so many happy plants & ever more happy customers... 11 years in & so different from my training in carpentry..
Keep up the good work & love your posts, thank you 🌻
I don't know why the youtube gods sent this to my feed, I'm more smoked brisket, golf, music and approaching retirement..........but I'm all in. You're awesome🥳
UA-cam algorithm on point! Terrific content.
I am not a biologist by ANY means but i do love plants and im very interested in learning tissue culture. I wish i had a friend that was into too so we could learn together.
It’s great to see a you beautiful woman who can be so smart and fun and not just take selfies.
I am a lover and a collector of rare succulents and this is a cloning technique that interests me a lot. In recent years I have collected a lot of information but your video is a perfect synthesis of everything that is needed to carry out this project. Great job and thank you so much! 🙂👍
P.s. I subscribed to your channel even before seeing that the channel is all about this technique! Fabulous! Bravissima! 👍👍👍
I'm a lover too ❤
Thank you so much I need to do this for my business and you are a pleasure to watch. You did a great job on something i really needed and I appreciate it a lot. Its been a year I hope you finger is better way to be a trooper and just do the video anyway... they dont make em like you everyday. Good Form. Thanks.
The kids ugh😂 . Fantastic video. I hope your hand heals up quickly. If you peel back the scalpel wrapper to just expose the attachment the cardboard and foil is usually enough to grip onto without risking shoving it through your hand.
Wish I knew that before I impaled myself!
Hello there, your teaching process is AWESOME 👍.
You are one of the best teacher in world. I am BSc (Botany) graduated. I found this video very helpful. I am from INDIA. If I was in USA I would like to meet you. You had a injury Take care. Thank you.
Awesome video, very informative for anyone who wants to try it! The only thing I would recommend is putting any lids face down, they’re less likely to get contaminated that way as the rim is usually exposed to the environment and any contaminating microorganisms on the counter wouldn’t make it to the top of the lid before you re-seal. Just make sure your work surface is clean (which you did). Bleach has “staying power” from the residue it leaves behind so it will continue killing microorganisms on the surface for a bit after cleaning.
I wasn't interested in this, so i didn't expect to actually watch this video. Your passion and energy kept me watching though. What you're doing is very interesting and cool.
It's great that you're introducing this to the greater community, but as a scientist with over 30 years of experience in immunology, biochemistry, and molecular biology, I highly recommend anyone trying this out to watch as many safety videos out there on handling flammable, toxic, and/or explosive chemicals as well as handling sharp objects (for surgical instruments). You don't want to physically hurt or poison yourself or burn your house down.
Hi Laur! I like your channel! Educative and easy to follow. You really now what you are doing what is great! I wish there where more interesting channels like yours and yes I am in my 40's but I know who Ana Montana is. I am a very well informed man in my 40's! I think is great that many other men my age are interesting in what you are doing! Men love plants too!😝😉😉
oh god no i just finished my carnivorous plants setup and have no space at all anymore but now i got recommended your video and the plant nerd in me REALLY got a craving to start my own lab lmao
Clone them!!! Why have 10 plants when you can have 10,000 plants? (And make money selling them hehe)
@@plantsinjars i'll just put the vid into my plants playlist and come back to it in like 5 years when i have a bigger apartment 🤣
Awesome video. Seems like something I would love to get into. My favorite quote was "Plants are Science." :)
Always wanted to get into plant tissue culture, and as an amateur Mycologist for years now, who would have known that there are only a few differences! You’ve inspired me to get a batch going this week
I love the evil finger tap but with the cast lol
I use nuroot, and any knife. 9/10 root. And I have a 97% rate of successful graphing using long razor graphs. I like my 10 in one trees. I will say lemons and grapefruit make a beast of a monster.
This is not too far off a mushroom growing set up . I can’t wait I’m going to binge your videos and buy a kit . I hope you heal quickly . Those blades are tricky I almost got my self a few times .
This was helpful after watching a few times. Thank you plants in jars. I'm going to culture some orchids.
I recently built my own laminar flow hood, it was really hard to find the right filter and fan here in Europe, but I'm happy with the results. It "only" costed about 400€. I still plan to upgrade to a professional one once I manage to find one for sale...
Thank you so much for sharing this!!! Knowledge is a powerful tool especially when it comes to saving genetics
This was such a fascinating subject to me when I took greenhouse horticulture in the mid 80's. The text book at the time was Plant Propagation principles and practices, chapter 17. In there it gives one the compounds and measurements for some stock solutions like the Murashigo & Skoog, the Gamorg, the Anderson and a woody plant medium. Of course each stock solution concentrate will make 100L of final solution.
Another fascinating read which I've read more than once even though I never did continue into the industry was by Lydiane Kyte, Timber Press called Plants from Test Tubes, An introduction to Micropropagation. I see it is still offered but now is in its 4th edition (2013). I'm sure a lot of progress has occurred in the 40 years since first publication. The index offers the make up of many early stock solutions including M&S.
Ooo that smile coupled wit plants... Nice...
props to you on doing this in a cast. Thats dedication!!!