Genius idea!! 🤯 How important is it to make media and sterilize it right away? Would it affect the media at all to leave that jug in the fridge for a day or two (and hope nobody tries to drink it 😅)
Giving this method a try now. Thank you for sharing. Any concern with using RO water? It comes out of my filter right around the target pH. EDIT - I re-watched the video, you mention reverse osmosis is OK around 0:55
Can you please explain that the ms media that you used in the video is in the powder form, but I couldn't find it in powder form on your Plant Cell Technology's website. On the website, MS Basal medium is available in liquid state. So, my question is, is there any difference or just same
Uh oh! Yes, our media doesn't contain sucrose. It's mentioned on the website and will also be written in the manual. "MURASHIGE & SKOOG MEDIUM (W/ VITAMINS & CALCIUM CHLORIDE, AGAR)"
for initiation phase, it's always media with agar, but at multiplication and rooting phase, that we perform in Biocoupler, it's only media with hormones, without agar.
You must not have mixed the agar well with the media. You need to ensure that you mix it well before sterilizing and then swirl the media in the bottle a bit before pouring in the vessels.
PGRs are added at the multiplication and rooting phase of plants. Generaly, the initiatin media doesn't involve PGR. But, it totally depends on the protocol you follow for your plant.
Thanks full information sir one by one name tell us and mixed tissue culture media how can make thanks brother please again home i shall work at home and be ready
We have many videos on our channel on media preparation that you can watch to learn more about the process as a beginner. If you face any problems, you can visit our website and learn from the written blogs that we have specially curated for our culturists.
Francisco, where do you get those containers? The ones we got from Karat melt in our autoclave.
They're the Round Snap-Lock Containers from our PCT Store, you can find them here: www.plantcelltechnology.com/round-snap-lock-containers/
Genius idea!! 🤯
How important is it to make media and sterilize it right away? Would it affect the media at all to leave that jug in the fridge for a day or two (and hope nobody tries to drink it 😅)
Hi. I have a quick question. Can i take a small tissue culture plant with roots puchhased from ebay and put it a biocoupler or would there be issues
Giving this method a try now. Thank you for sharing. Any concern with using RO water? It comes out of my filter right around the target pH.
EDIT - I re-watched the video, you mention reverse osmosis is OK around 0:55
That's great!
Can you please explain that the ms media that you used in the video is in the powder form, but I couldn't find it in powder form on your Plant Cell Technology's website. On the website, MS Basal medium is available in liquid state.
So, my question is, is there any difference or just same
Does plant cell technology’s ms media not have sucrose? Oh no my plants I just TC’d might be In Trouble 😢
Uh oh! Yes, our media doesn't contain sucrose. It's mentioned on the website and will also be written in the manual. "MURASHIGE & SKOOG MEDIUM (W/ VITAMINS & CALCIUM CHLORIDE, AGAR)"
You are not alone! I just made this same mistake with my last batch, too.
I have a question. Do you use the skoog with agar? Or do you use it by itself?
for initiation phase, it's always media with agar, but at multiplication and rooting phase, that we perform in Biocoupler, it's only media with hormones, without agar.
Thank you for the help. I appreciate it
@@JordonCope Happy to help!
Hello..thx for the video...
But i've a qu...
I made 100ml of ms media..use agar about 0.8gm .but it didn't make solid ...why is that..??
You must not have mixed the agar well with the media. You need to ensure that you mix it well before sterilizing and then swirl the media in the bottle a bit before pouring in the vessels.
If the Agar doesn't gell up what do I need to do? Add more agar?
How much agar do you use in 1 L of media?
4.5 grams of ms medium?
Murishige and skoog with vitamins?
yes, that's right!
Will my plants die if I did not add sugar to the PCT media?
Yes, sugar is the source of carbon for plants. So, you need to add it to the tissue culture media.
Thanks, Just re-did all my cultures
@@PlantCellTechnology
why aren't you not adding any pgrs?
PGRs are added at the multiplication and rooting phase of plants. Generaly, the initiatin media doesn't involve PGR. But, it totally depends on the protocol you follow for your plant.
Thanks full information sir one by one name tell us and mixed tissue culture media how can make thanks brother please again home i shall work at home and be ready
We have many videos on our channel on media preparation that you can watch to learn more about the process as a beginner. If you face any problems, you can visit our website and learn from the written blogs that we have specially curated for our culturists.
Why my media is solid?
Solid media results from adding agar or gellan gum (a solidifying agent) to the media.
@@PlantCellTechnology thank you
you are to far from the mic
So counting 20 drops is easier than the flick of a thumb? No thanks I'll continue to use my metered pipette before I'm counting drops.
you know? why say anything at all?
It's easier to buy pre-made media...
Perspective, tools-on-hand, and budget are all factors that determine the meaning of "easy."