I'm an American plant junkie that's been living here in Thailand......If there are any plants you want from here...just let me know. I've been dying to learn tissue culture forever....so I'm your fan-boy! A very old fan-boy.
Regarding C. sativa, you can always do a demonstration with industrial hemp. It grows like a weed (pun intended) here in Texas. It sticks out once you learn to recognize it.
Your videos have popped up a few times on my feed and I have enjoyed them. Brings back memories as I have a degree in Mycology (mushrooms) and a lot of what you do, I've done in the past. For those suggesting you do mushrooms, I say go for it. You can do tissue culture from most parts of the mushroom, just make sure you sample from an interior area protected from any contaminants. Standard medium is PDA (Potato Dextrose Agar) The recipe is available on lots of sites. Personally, I would like to see you do it because it will highlight where you need to improve in your sterile work under the flow hood. PDA will grow all fungus, molds, and yeast so one small mistake will lead to a contamination of the plate. It took me 100's of plates to get my contamination down to below 2%. ;)
Hello @GrandpasPlace ! I am a frustrated marine mycologist, I say frustrated because my field is actually fish physiology. I wanted to shift to marine mycology but the institution I worked with did not allow me to. Nice to see a veteran mycologist here.
17:52 variegated bananas are very profitable and should be quite easy considering here in Central Florida (Lakeland) where I live one plant can become a large clump in just about 2 years time outdoors with no care once you find The Sweet Spot
Fascinating, digestible and informative. A subject I have never been exposed to, but I am very much enjoying your tutorials. Also, love your philosophy.
As soon as you mentioned mycorrhizal fungi networks I was like "oh yeah nice we'd get along great!", then you said you don't like mushrooms. I'm not sure what to tell you now. Jokes aside, I love this channel. I've learned so much. I've been propagating aquatic plants using various hydroponic systems, but I've been struggling with propagating enough of certain plants. I'm realizing this might be the answer to mass-production of babies to grow out, even if the initial time input might be significant. The scalability seems incredible! I'm also familiar with mycelium culturing and have the equipment and procedures figured out, so hopefully it transfers over well. Thanks for putting this information out there! I hope your finger's healed, I just watched that scalpel accident video.
PLEASE call it "Audrey II" for a few reasons. 1. Monster plant. 2. This is your second try. 3. You get to say "Feed me Seymour" in a fun voice 😂 This is a reference to 'little shop of horrors' for any who don't get it. It's a play about a Monster plant
I just came across this video in my recommended, and when you read the question at 10:20, I was literally thinking about the Lyon Arboretum at UH Mānoa. They're doing really important work to keep the plants my ancestors once used! It's just a shame we can't do the same for our endemic fauna--at least not in the same way.
i visited epcot maybe 10y ago and did the same tour it was amazing also i have been doing hydroponics and mycology work for some time which is now leading into aquaponics and tissue culture so am finding your channel very interesting and love that u are so beginner/hobbyist friendly keep up the good work and thank you
Glad to see you’re hand is doing better. I’ve been there. Cut muscles and and tendons in my hand several times. Love plants and I love you teaching how to propagate!🌱
I started tissue culture 2 years ago and I did tens of attempts and everything was a complete failure until today :( But I never give up and I'm still striving to see a sprouted explant! The interesting fact is that I don't have any fungal contamination (0% fungal) and the only thing that's ruining everything is endogenous and exogenous bacterial contamination from my explant! The last time I almost killed most of my explants in the sterilisation process but again I got contamination from the explants 😭
Try growing your plants that you cut off of sterile , grow with hydrogen or oxide, spray the plant with wet table sulphur to clean it with , before you take your cuts spray the plants down well with hydrogen peroxide/ water mix , 35 percent hydrogen peroxide at 10 mls a gallon. Then cut your material from that plant. This will help a lot getting your tissue through clean.
Thank you for your amazing work! Here two ideas for future videos: 1) what about safety in using hormones and how to deal with disposables? Do you have to sterilize them after use? 2) from what I understand you are reproducing mainly indoor plants, what about making tissue culture with conifers? Is it possible?
Neat! First video of yours I've watched. I'd love to try it but my hands shake too much. About schools: in 2014 I sold my business and went back to school. Now up to 5 degrees. Next is Ms in Biology and BS in Hort. You can do it too. Go for it. If you have questions, ask.
You may not personally like the taste of mushrooms, but you can make a profit selling them. Gourmet/rare culinary mushrooms and medicinal mushrooms can be grown from free materials like used coffee grounds from local coffee shops, or wood chips discarded by arborist companies. I know it's a lot of work running your own business, just an idea if/when you're looking to expend your horizons.
Yo! You went to UF with me, I just realized when you mentioned the Plants Gardening and You class 😂 Dr. Clark was amazing. I wish you had the experience of majoring in Botany, Ag, or microbio, because UF STEM is legit! But you obviously didn’t need it, natural genius
Love your channel and your content no BS just great information. I do have some questions about why you do some things or choose some plants. If it’s because they are your favorites, I can totally get on board with that however, if this is a special strain of begonia, I’m also super interested in that as well. I guess I’m asking why do you choose some projects or how do you choose the projects that you’re gonna get started on
I absolutely love that you’ve created a whole business out of this. I definitely assumed you had a biology degree of sorts, so double kudos to you for learning all of this yourself. I will say, I’m quite sad that you’ve switched to disposable containers. I’m not sure what the benefit is since you still have to sterilize them. And it’s just so much unnecessary plastic waste 😞. Nonetheless, love your videos and can’t wait to see the next one!
There’s allot of benifit in using plastic including not having to expose yourself to contamination to clean you just throw them out, at a exponential size it can be very expensive to invest in the same amount of mason jars and lids etc
So I damaged my tendon with MRSA and I can no longer do a good portion of things especially because it is my index. The tendon branches off. So it's important to use your hand. Keep at it 😊
I found your content extremely informative. I look forward to your future content. I've only had a chance to view 2 of your videos. I looked at your etsy store and would be happy to shop there for rare tropicals, sorry not a begonia fan. I am assuming the Thia Constellation was tissue cultured and hope to find that in one of your videos. You had at least a 1/2 dozen including some Hoya, Burle Marx I am still in the market for. I have been successful with cuttings. Like I said I can't wait for you to finish your series. I subscribe to a few channels and would love to see weekly content. Too bad TC takes so long I came across an affordable batch of Spritus Sancti, but with my luck, the price will have dropped considerably before the plants are viable and I don't have the luxury of your weather living in MI. TC works for me with rarer plants because you can buy anything of real size here. A 4ft monstera is nearly $100 and if you try to ship larger plants they just get damaged. I put almost all my large plants on the balcony for the summer and they grow so well I don't care how much you spend on lights nothing beats mother nature. Come fall everything has to be treated as organically as possible for pests repeatedly I lost half my collection in 2 weeks at the start not keeping a close enough eye on thrips and spider mites. Now I know which big box stores sell plants that really attract them so I go buy some sacrificial ones that I really keep an eye on and place them just outside the main growing areas which has worked well and the plants can handle extra pest treatments better than some of the harder to grow plants. I am amazed that you didn't study botany in college. I do agree with you having shopped Thailand I came across a number of sites that had much cheaper shipping for tissue culture for rare tropicals. The one site would have shipped full-size mother plants for what you were charged. I guess I will have to sign up with a few social media platforms rather than just here if I am going to be able to follow you. I appreciate all the extra information you include like the names of a few places in the states doing tissue culture, where you can go to see it done. What better reason than to plan a trip to Hawaii.
Love the way you present science, like a cool family member than a grumpy teacher. Also checkout ayurvedic plants for propagation they have a huge demand too !!
Please helllp! 😭 I messed up! I accidentally added 30gr/L of agar instead of 6gr/L. I used an expensive DKW media and other expensive additives and hormones and I don't wanna waste it! Will this too high concentration of agar affects plant growth?
You are so funny! I am really impressed that someone with no science background has come so far in such a technical field. Having worked in a high end lab (virus culture, not tissue culture, but many of the techniques are the same), I am in awe at what you're able to accomplish on a shoestring budget. Keep up the great content! I did have a question about tissue culture of patented plants....How big of an issue is that? My first thought when I saw your Watermelon Snow was that was probably a patented variety, but in looking through a bunch of sources, I don't see plant patents listed as frequently as they once were. Not sure how you know if the plant you have is covered by a patent. By the way, accidental tendon damage like you experienced is absolutely no fun. Hope you continue to heal and get back to 100%. I had something similar happen a couple of years ago and my finger is no longer straight and refuses to contract as well as the others. Something you learn to live with. Thanks again for your videos!
One thing I never see mentioned is air during multiplication and rooting. Do containers need air hole and filter patch, and if so, what size hole. Only hole I have seen is on biocoupler.
I couldn't find in your videos what kind of paper you use to make the micropropagation in the sterile cabinet. do you use normal kitchen paper or another kind? do you sterilize the paper in the autoclave? how is it done? thank you very much for your videos and your work!
I wonder, is it possible to tissue culture a pepper plant from a store bought pepper? I know with Aloha peppers if you plant the seeds from them, you just get plain yellow peppers. It would be nice to be able to grow those things at home
Hello from Chile. I bring grow regulators from my stay in Europe, but they came in powder presentation. what is the best way to handle them or prepare stock solutions to work with? I love your channel and I am very excited to start in TC ^^
Hi question: can you grow micro cultures from other seeds? You mentioned growing orchids that way and I'm only able to find rubus chamaemorus seeds even though I've been looking for a plant/cutting/bareroot for over a year
I'm curious if tissue culture would be a better way to propagate specific cannabis cultivars in particular specific phenotypes. Because traditional methods of producing clones, as far as I'm aware are by keeping one or multiple mother plants and taking cuttings then rooting those in rooting hormone but it is a process that often makes the plants more unstable in their growth pattern as I understand it, in particular not developing a vertical main stem that branches out evenly, but results in plants that have to be "monster cropped" and makes the care and training a lot more complicated and labor intensive. How does the quality of plants grown from tissue culture differ from cloning standard cuts? Does it affect the resilience differently? It's another topic of debate in the cannabis community that I haven't found a clear scientific answer to, some people claim cloning have the same exact genetics but others claim the more you clone the same plant the weaker the resilience gets. Isn't the exact phenotype preserved in all instances? Do the different means of propagation affect this differently do you know have any info on this?
Not plants in jail cells XD You could probably use non-active hemp plants used for fiber culture to bypass the law btw, just wouldn't be able to test flower quality
Hello Laur. Yes... I know you think I need a crash course in pop culture because I am on my forties and I don't know who is Hanna Montana. I'm absolutely loving that program from the University of Hawaii you told about. Preserving all plant species is so important, that every country should definitely hop on board before it's too late. Thanks for sharing your knowledge with me, Laur! I'm glad I get to learn all these amazing things with you! Some beautiful plants and flowers for you🏵🪴🌵☘🌺🌷😉😉
Hi, I’m new to this. And you mentioned somewhere at the end of Part II, that on that big glass beaker or jar that the plants are contaminated? What do you mean by contaminated? And how do you know it is contaminated? How do I find out that my specimen is contaminated and with what? I’m just learning tissue culture from you. Thanks
STOP - not the Plants, Gardening & You class! My tomato plant was STOLEN off my porch and I ended up getting a B in the class because of it 😭😂 but I also absolutely loved that class and took it for no related reason to my major too lmao
Typing in "Oh I hit my finger so hard".....Hmmmm, not the results I was expecting LOL Stumbled upon your channel, love the videos, not I'm gonna end up spending money for a home lab i didn't know I needed, but now really really want 😂
Is there any reason why you use two explants? Do you sell or sources where I can buy the tools required in plant culture? Can I store explants for sometime before use?
Great video, looking forward to you next one on acclimation. Quick question, can you show close ups of a callus? I'm trying to TC citrus, and I'm concerned that a callus can look a lot like contamination...
Have you ever tried to TC carnivorous plants like Nepenthes, Drosera, or Venus Flytrap? What, if anything, is different? My concern is the disinfecting the explant material before culturing. They seem to die easily if they come in contact with a variety of substances. You have to use 0 ppm tds water to water the plant, so I'm curious how this changes with the nutrient you use.
I have sundews multiplying in some BioCouplers right now. The main difference is that you want to use less MS media (I think you use 1/3rd the amount you would use for other houseplants).
@@plantsinjars That's awesome. Thanks. Out of curiosity, what parts of the Drosera do you cut for your explant material? Also... what does MS media stand for? 😅
Hi Laur. You mention that people from discord helped you for one of your begonia protocol. Are there any discord server dedicated to PTC enthusiasts that you know?
Thanks for the vid. What varieties seem to be most popular? Are you aware of any folks that do tissue culture for common edible plants, like herbs, fruits and veggies? Thanks. Cheers from N. FL!
I cringed when you hit your finger... I've only recently found your channel so the trauma is still very real here. Can I ask to what extent you need to be careful with waste containing the PGRs? The used media and waste water from washing the plants or washing containers, etc. How do you dispose of your troubled waste? Can't wait to watch you on this incredible journey. Many thanks from Sydney, Australia.
Tammy would work possibly for the name 😆. Thank you for your content! I have been recently getting into this. I have several pothos, and monstera I am planning on TC 🙂. Thank you so much for what you do!
Just a question, what discord? And how do you clean the plant after it has rooted? How do you root it? How do you clean up the containers after you're done? I'm guessing you can't just remove the gels using water, since you need to specially treat it. How do you clean the tools?
If for some reason you were travelling to Mars on a 6 month journey. On your arrival to Mars, you had to start growing plants for food. Grow rooms were already built by automated robots. Would you bring seeds and then do tissue culture when you arrive on the planet? Or would you start tissue culture when on your 6 month journey to the planet. Or would you do some tissue culture but leave some seeds to use if the culture plants didn’t survive the journey landing on Mars.
How often do you get Contamination? It's funny in mycology Trich is contamination for mushroom cultivation but in the plant world it's used in mycorrhizal relationships. Any thoughts?
I wish I could do tissue culture. I bought a kit, I used my microwave to sterilize everything. But every sample I did ended up infected I need a professional environment to do it right. I just have way too many spores. I live with a large yard full of plants, leaves and spores. I live in central Florida.
Autoclaving everything in a pressure cooker might help. The microwave isn't a perfect method, but pressure cooking at 15 PSI for 20 minutes should kill any microrganisms. I sometimes still get contamination even with the flow hood & autoclave
@@plantsinjars the problem is when you expose things to air which I have to do unless I am in a small plastic room with no spores I will probably end up contaminating most. So I spray everything with alcohol after I place stuff inside the large plastic tote. But still I get contamination. I will try with a pressure cooker next time. Have you ever done tissue culture with heliamphoras?
@@plantsinjars I have had some luck getting roots to form out of sundews and Australian pitcher plants. But heliamphoras never root their pitchers. And if you cut of the plant from the roots, the roots don’t put out new pitchers. Only the plant will grow new roots. It would be nice if I could tissue culture them. I bought a plant for 800.00 and it died on me two months later. I think I over fertilized it.
I'm an American plant junkie that's been living here in Thailand......If there are any plants you want from here...just let me know. I've been dying to learn tissue culture forever....so I'm your fan-boy! A very old fan-boy.
Please do not send wild plants across intercontinental countries. That's how plant disease and parasites spread.
Bro, this is what YT was made for. Love the channel
As a YT lady. I approve of this comment
Your passion is contagious. These days I have to decide whether to begin a phd on tissue culture or not, and your videos make me wanna go with it!
If you do, join my discord server to spread the tissue culture knowledge!
Regarding C. sativa, you can always do a demonstration with industrial hemp. It grows like a weed (pun intended) here in Texas. It sticks out once you learn to recognize it.
I'm joining the tissue culture journey! I ordered everything and more off of your starting list. 🎉
Keep me updated on how it goes!
Keep US updated ❤
Update?
Your videos have popped up a few times on my feed and I have enjoyed them. Brings back memories as I have a degree in Mycology (mushrooms) and a lot of what you do, I've done in the past. For those suggesting you do mushrooms, I say go for it. You can do tissue culture from most parts of the mushroom, just make sure you sample from an interior area protected from any contaminants. Standard medium is PDA (Potato Dextrose Agar) The recipe is available on lots of sites.
Personally, I would like to see you do it because it will highlight where you need to improve in your sterile work under the flow hood. PDA will grow all fungus, molds, and yeast so one small mistake will lead to a contamination of the plate. It took me 100's of plates to get my contamination down to below 2%. ;)
Hello @GrandpasPlace ! I am a frustrated marine mycologist, I say frustrated because my field is actually fish physiology. I wanted to shift to marine mycology but the institution I worked with did not allow me to. Nice to see a veteran mycologist here.
Its so nice to find another person talking about begonias and african violets that isn't 3x older than me. Love the content!
i love your videos, and you have been my main motivation to open a small business using tissue culture
That is so awesome. Thank you!
Bringing down Big Plant ....
The empire is expanding...
Looking forward to the in-depth videos on organogenesis and somatic embryogenesis!
Theyre already available on her onlyfans lol 🤣
Underrated comment 😂😂😂
Great work! Thanks to you I I have been grow strawberry in tissue culture for a month now. I have only 15 jar, but they grow excellent!
17:52 variegated bananas are very profitable and should be quite easy considering here in Central Florida (Lakeland) where I live one plant can become a large clump in just about 2 years time outdoors with no care once you find The Sweet Spot
Whats your disposal protocol for media/disposable containers?
Fascinating, digestible and informative. A subject I have never been exposed to, but I am very much enjoying your tutorials. Also, love your philosophy.
As soon as you mentioned mycorrhizal fungi networks I was like "oh yeah nice we'd get along great!", then you said you don't like mushrooms.
I'm not sure what to tell you now.
Jokes aside, I love this channel. I've learned so much. I've been propagating aquatic plants using various hydroponic systems, but I've been struggling with propagating enough of certain plants. I'm realizing this might be the answer to mass-production of babies to grow out, even if the initial time input might be significant. The scalability seems incredible! I'm also familiar with mycelium culturing and have the equipment and procedures figured out, so hopefully it transfers over well. Thanks for putting this information out there! I hope your finger's healed, I just watched that scalpel accident video.
PLEASE call it "Audrey II" for a few reasons.
1. Monster plant.
2. This is your second try.
3. You get to say "Feed me Seymour" in a fun voice 😂
This is a reference to 'little shop of horrors' for any who don't get it. It's a play about a Monster plant
I just came across this video in my recommended, and when you read the question at 10:20, I was literally thinking about the Lyon Arboretum at UH Mānoa. They're doing really important work to keep the plants my ancestors once used!
It's just a shame we can't do the same for our endemic fauna--at least not in the same way.
i visited epcot maybe 10y ago and did the same tour it was amazing also i have been doing hydroponics and mycology work for some time which is now leading into aquaponics and tissue culture so am finding your channel very interesting and love that u are so beginner/hobbyist friendly keep up the good work and thank you
I'm all ears, your character and plant passion makes me tingle
Glad to see you’re hand is doing better. I’ve been there. Cut muscles and and tendons in my hand several times. Love plants and I love you teaching how to propagate!🌱
I started tissue culture 2 years ago and I did tens of attempts and everything was a complete failure until today :(
But I never give up and I'm still striving to see a sprouted explant!
The interesting fact is that I don't have any fungal contamination (0% fungal) and the only thing that's ruining everything is endogenous and exogenous bacterial contamination from my explant! The last time I almost killed most of my explants in the sterilisation process but again I got contamination from the explants 😭
Try growing your plants that you cut off of sterile , grow with hydrogen or oxide, spray the plant with wet table sulphur to clean it with , before you take your cuts spray the plants down well with hydrogen peroxide/ water mix , 35 percent hydrogen peroxide at 10 mls a gallon. Then cut your material from that plant. This will help a lot getting your tissue through clean.
Thank you for your amazing work! Here two ideas for future videos: 1) what about safety in using hormones and how to deal with disposables? Do you have to sterilize them after use? 2) from what I understand you are reproducing mainly indoor plants, what about making tissue culture with conifers? Is it possible?
You have the gift of teaching :)
Another amazing video, chock full of information. Every minute is educational, entertaining, or both!
Neat! First video of yours I've watched. I'd love to try it but my hands shake too much.
About schools: in 2014 I sold my business and went back to school. Now up to 5 degrees. Next is Ms in Biology and BS in Hort. You can do it too. Go for it. If you have questions, ask.
You may not personally like the taste of mushrooms, but you can make a profit selling them. Gourmet/rare culinary mushrooms and medicinal mushrooms can be grown from free materials like used coffee grounds from local coffee shops, or wood chips discarded by arborist companies. I know it's a lot of work running your own business, just an idea if/when you're looking to expend your horizons.
You are helping me to learn alit about this technique. Thank you !
Hie my sister I really happy to see your practical nice works, God bless you
Yo! You went to UF with me, I just realized when you mentioned the Plants Gardening and You class 😂 Dr. Clark was amazing. I wish you had the experience of majoring in Botany, Ag, or microbio, because UF STEM is legit! But you obviously didn’t need it, natural genius
I really like plants but could really watch you talk about anything 😊
great lesson , thank you :) your every video inspiring me towards tissue culture
You can try to grow a fluorescent mushroom it's very pretty
I think you can reach that goal, rooting for you 🎉
Love your channel and your content no BS just great information. I do have some questions about why you do some things or choose some plants. If it’s because they are your favorites, I can totally get on board with that however, if this is a special strain of begonia, I’m also super interested in that as well. I guess I’m asking why do you choose some projects or how do you choose the projects that you’re gonna get started on
you are too cool for doing this series!
This is totally off topic, but you are SO CUTE and I love your hairstyle! 🥰
I absolutely love that you’ve created a whole business out of this. I definitely assumed you had a biology degree of sorts, so double kudos to you for learning all of this yourself. I will say, I’m quite sad that you’ve switched to disposable containers. I’m not sure what the benefit is since you still have to sterilize them. And it’s just so much unnecessary plastic waste 😞. Nonetheless, love your videos and can’t wait to see the next one!
There’s allot of benifit in using plastic including not having to expose yourself to contamination to clean you just throw them out, at a exponential size it can be very expensive to invest in the same amount of mason jars and lids etc
We need you in the cannabis industry ❤ thanks for the nice content
So I damaged my tendon with MRSA and I can no longer do a good portion of things especially because it is my index. The tendon branches off. So it's important to use your hand. Keep at it 😊
Love your videos! Super interesting! :)
I found your content extremely informative. I look forward to your future content. I've only had a chance to view 2 of your videos. I looked at your etsy store and would be happy to shop there for rare tropicals, sorry not a begonia fan. I am assuming the Thia Constellation was tissue cultured and hope to find that in one of your videos. You had at least a 1/2 dozen including some Hoya, Burle Marx I am still in the market for. I have been successful with cuttings. Like I said I can't wait for you to finish your series. I subscribe to a few channels and would love to see weekly content. Too bad TC takes so long I came across an affordable batch of Spritus Sancti, but with my luck, the price will have dropped considerably before the plants are viable and I don't have the luxury of your weather living in MI. TC works for me with rarer plants because you can buy anything of real size here. A 4ft monstera is nearly $100 and if you try to ship larger plants they just get damaged. I put almost all my large plants on the balcony for the summer and they grow so well I don't care how much you spend on lights nothing beats mother nature. Come fall everything has to be treated as organically as possible for pests repeatedly I lost half my collection in 2 weeks at the start not keeping a close enough eye on thrips and spider mites. Now I know which big box stores sell plants that really attract them so I go buy some sacrificial ones that I really keep an eye on and place them just outside the main growing areas which has worked well and the plants can handle extra pest treatments better than some of the harder to grow plants. I am amazed that you didn't study botany in college. I do agree with you having shopped Thailand I came across a number of sites that had much cheaper shipping for tissue culture for rare tropicals. The one site would have shipped full-size mother plants for what you were charged. I guess I will have to sign up with a few social media platforms rather than just here if I am going to be able to follow you. I appreciate all the extra information you include like the names of a few places in the states doing tissue culture, where you can go to see it done. What better reason than to plan a trip to Hawaii.
Love the way you present science, like a cool family member than a grumpy teacher. Also checkout ayurvedic plants for propagation they have a huge demand too !!
You can compensate the mushroom eating with Soya Sauce. The chinese variety. *nod nod*
Yo you are an absolute gangster! Love your energy and information! Also, thanks for that link in the description 😅
Please helllp! 😭 I messed up!
I accidentally added 30gr/L of agar instead of 6gr/L. I used an expensive DKW media and other expensive additives and hormones and I don't wanna waste it!
Will this too high concentration of agar affects plant growth?
You are so funny!
I am really impressed that someone with no science background has come so far in such a technical field. Having worked in a high end lab (virus culture, not tissue culture, but many of the techniques are the same), I am in awe at what you're able to accomplish on a shoestring budget. Keep up the great content!
I did have a question about tissue culture of patented plants....How big of an issue is that? My first thought when I saw your Watermelon Snow was that was probably a patented variety, but in looking through a bunch of sources, I don't see plant patents listed as frequently as they once were. Not sure how you know if the plant you have is covered by a patent.
By the way, accidental tendon damage like you experienced is absolutely no fun. Hope you continue to heal and get back to 100%. I had something similar happen a couple of years ago and my finger is no longer straight and refuses to contract as well as the others. Something you learn to live with.
Thanks again for your videos!
One thing I never see mentioned is air during multiplication and rooting. Do containers need air hole and filter patch, and if so, what size hole. Only hole I have seen is on biocoupler.
Hail the K-Cup tech 😁 I grow mushrooms and any savings I can use I take advantage of!
I couldn't find in your videos what kind of paper you use to make the micropropagation in the sterile cabinet. do you use normal kitchen paper or another kind? do you sterilize the paper in the autoclave? how is it done? thank you very much for your videos and your work!
Have you ever used tissue culture to remove plant viruses? Thanks for sharing all your TC knowledge!
I wonder, is it possible to tissue culture a pepper plant from a store bought pepper? I know with Aloha peppers if you plant the seeds from them, you just get plain yellow peppers. It would be nice to be able to grow those things at home
plants in jailcells 😭😭😭😭
This was the comment that took me out 😂😂😂
Thanks for the tree info, Much apreciated
I’m guna need you to upload way more often! Please. I need to learn all of this and I’m a binge watcher type lol I need all the videos 2maro
im in love with you and your knowledge!
I would want more people like you in tissue culture since I want to find a way to get Wheat and Corn in hydroponics
Hello from Chile. I bring grow regulators from my stay in Europe, but they came in powder presentation. what is the best way to handle them or prepare stock solutions to work with?
I love your channel and I am very excited to start in TC ^^
Do you do mycology as well? Super interested in propagating morels!
Question: do all tissue cultures require the same lumens/foot candles?
I grow mushrooms and find myself drawn to plant tissue culturing. I want to create a permaculture home someday.
LMAO don’t come for me at 8:36 😂😂😂
Hi question: can you grow micro cultures from other seeds? You mentioned growing orchids that way and I'm only able to find rubus chamaemorus seeds even though I've been looking for a plant/cutting/bareroot for over a year
I'm curious if tissue culture would be a better way to propagate specific cannabis cultivars in particular specific phenotypes. Because traditional methods of producing clones, as far as I'm aware are by keeping one or multiple mother plants and taking cuttings then rooting those in rooting hormone but it is a process that often makes the plants more unstable in their growth pattern as I understand it, in particular not developing a vertical main stem that branches out evenly, but results in plants that have to be "monster cropped" and makes the care and training a lot more complicated and labor intensive. How does the quality of plants grown from tissue culture differ from cloning standard cuts? Does it affect the resilience differently? It's another topic of debate in the cannabis community that I haven't found a clear scientific answer to, some people claim cloning have the same exact genetics but others claim the more you clone the same plant the weaker the resilience gets. Isn't the exact phenotype preserved in all instances? Do the different means of propagation affect this differently do you know have any info on this?
Not plants in jail cells XD You could probably use non-active hemp plants used for fiber culture to bypass the law btw, just wouldn't be able to test flower quality
Do you want to become Poison Ivy? Because this is how you become Poison Ivy. Use your powers for good. 😁
So dig this channel thank you.
Can you keep them in there in the jars forever? And when they grow to big split them up? Or do they “mature” and need to be transplanted eventually
Call it the Frankencutlure or saladculture jar, I'm leaning towards Salad culture🤪
This is fascinating.
Try glowing mushrooms
I'm following you to do this with my cannabis cultivars I breed...
Hello Laur. Yes... I know you think I need a crash course in pop culture because I am on my forties and I don't know who is Hanna Montana. I'm absolutely loving that program from the University of Hawaii you told about. Preserving all plant species is so important, that every country should definitely hop on board before it's too late. Thanks for sharing your knowledge with me, Laur! I'm glad I get to learn all these amazing things with you! Some beautiful plants and flowers for you🏵🪴🌵☘🌺🌷😉😉
Hi, I’m new to this. And you mentioned somewhere at the end of Part II, that on that big glass beaker or jar that the plants are contaminated? What do you mean by contaminated? And how do you know it is contaminated? How do I find out that my specimen is contaminated and with what? I’m just learning tissue culture from you. Thanks
For Kryo temperatures - USE LIQUID NITROGEN
STOP - not the Plants, Gardening & You class! My tomato plant was STOLEN off my porch and I ended up getting a B in the class because of it 😭😂 but I also absolutely loved that class and took it for no related reason to my major too lmao
Nice to see you're focusing on b2b orders now. How's it going for you?
Typing in "Oh I hit my finger so hard".....Hmmmm, not the results I was expecting LOL
Stumbled upon your channel, love the videos, not I'm gonna end up spending money for a home lab i didn't know I needed, but now really really want 😂
Is there any reason why you use two explants? Do you sell or sources where I can buy the tools required in plant culture? Can I store explants for sometime before use?
Did you ever get the synthetic seeds to germinate?
So, you can take one little piece of a plant and it grows into a bunch of little plants? I need to know more!
AYO Fellow Begonia lover! heck yeah!
Great video, looking forward to you next one on acclimation. Quick question, can you show close ups of a callus? I'm trying to TC citrus, and I'm concerned that a callus can look a lot like contamination...
Contamination will grow in the culture ,
Hey, can you please share the model and make of your Flowhood?
Have you ever tried to TC carnivorous plants like Nepenthes, Drosera, or Venus Flytrap? What, if anything, is different? My concern is the disinfecting the explant material before culturing. They seem to die easily if they come in contact with a variety of substances. You have to use 0 ppm tds water to water the plant, so I'm curious how this changes with the nutrient you use.
I have sundews multiplying in some BioCouplers right now. The main difference is that you want to use less MS media (I think you use 1/3rd the amount you would use for other houseplants).
@@plantsinjars That's awesome. Thanks. Out of curiosity, what parts of the Drosera do you cut for your explant material? Also... what does MS media stand for? 😅
Which light timer do you use?
Nice work
Laur i love you 🥰🥰🥰🥰
19:26 my neighbor just got arrested for growing pot (I guess the property line is not where I thought it was 😊😅😂)
Hi Laur. You mention that people from discord helped you for one of your begonia protocol. Are there any discord server dedicated to PTC enthusiasts that you know?
Name the giant flask “Big Birtha”!! 😂
Thanks for the vid. What varieties seem to be most popular? Are you aware of any folks that do tissue culture for common edible plants, like herbs, fruits and veggies? Thanks. Cheers from N. FL!
If you decide to do woody plants. I have a small-ish pine tree thats worth growing for pine nuts. Would it be possible to try Pinus pumila?
I cringed when you hit your finger... I've only recently found your channel so the trauma is still very real here. Can I ask to what extent you need to be careful with waste containing the PGRs? The used media and waste water from washing the plants or washing containers, etc. How do you dispose of your troubled waste? Can't wait to watch you on this incredible journey.
Many thanks from Sydney, Australia.
Tammy would work possibly for the name 😆. Thank you for your content! I have been recently getting into this. I have several pothos, and monstera I am planning on TC 🙂. Thank you so much for what you do!
What size are those deli containers you use??
Can you sterilize with an instant pot? I assume so just don't know how long to keep them in.
Just a question, what discord? And how do you clean the plant after it has rooted? How do you root it? How do you clean up the containers after you're done? I'm guessing you can't just remove the gels using water, since you need to specially treat it. How do you clean the tools?
If for some reason you were travelling to Mars on a 6 month journey.
On your arrival to Mars, you had to start growing plants for food.
Grow rooms were already built by automated robots.
Would you bring seeds and then do tissue culture when you arrive on the planet? Or would you start tissue culture when on your 6 month journey to the planet.
Or would you do some tissue culture but leave some seeds to use if the culture plants didn’t survive the journey landing on Mars.
How often do you get Contamination? It's funny in mycology Trich is contamination for mushroom cultivation but in the plant world it's used in mycorrhizal relationships. Any thoughts?
Usually around 10% or so get contaminated. Trying to get that number as low as possible though
I wish I could do tissue culture. I bought a kit, I used my microwave to sterilize everything. But every sample I did ended up infected
I need a professional environment to do it right.
I just have way too many spores. I live with a large yard full of plants, leaves and spores.
I live in central Florida.
Autoclaving everything in a pressure cooker might help. The microwave isn't a perfect method, but pressure cooking at 15 PSI for 20 minutes should kill any microrganisms. I sometimes still get contamination even with the flow hood & autoclave
@@plantsinjars the problem is when you expose things to air which I have to do unless I am in a small plastic room with no spores I will probably end up contaminating most. So I spray everything with alcohol after I place stuff inside the large plastic tote. But still I get contamination.
I will try with a pressure cooker next time.
Have you ever done tissue culture with heliamphoras?
@@samuelmiensinompe4902 I don't but I actually just ordered some sundews which will be my first carnivorous plants
@@plantsinjars I have had some luck getting roots to form out of sundews and Australian pitcher plants. But heliamphoras never root their pitchers. And if you cut of the plant from the roots, the roots don’t put out new pitchers. Only the plant will grow new roots.
It would be nice if I could tissue culture them. I bought a plant for 800.00 and it died on me two months later. I think I over fertilized it.
@@plantsinjarscan you grow Venus fly traps like you do?
There's some cool mushrooms out there some even glow in the dark
how do you tell the jar has been contaminated?
😍🤗 sayin rooting lots of times sounds like a naughty Australian lol :D