mRNA Splicing
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- Опубліковано 2 бер 2008
- NDSU Virtual Cell Animations Project animation 'mRNA Splicing'. For more information please see vcell.ndsu.edu/animations
Before being used in translation, mRNA must be spliced. During splicing, introns are removed and the translatable exons that remain are spliced into a single strand of mRNA.
I cried when the intron faded away at the end
Mr. ribosome I dont feel so good
😂
U still alive?
I learned more from that 2 minute video, than the 100 min lecture that my professor gave.
Haha
hey u still alive?
I want to ask the same Q😭😭 @@noblevenom2858
How many of you just went "OHHHHHH" after watching this? i know i did.
WHOS WATCHING IN 2020 LOLMAO
@@potassium7794 this should be a high rank animation in 2008
do u get paid to write this
@@gracewalker3300 idk lol
but that's sus
Mee
U1 binds to 5' splice site, U2 binds to the A branch. After they've been rearranged U1 and U4 dissociate. U2 and U6 have the catalytic function with regards to the transesterification reactions.
Thanks the way you worded it really helped
What is the small droplets type thing that strikes to the various proteins in animation ??
@@thakurprathvisingh4048 I believe it's ATP, which should be needed for the complex formation and activation
@@drosophilamelanogaster933 thank you 😊 ☺
it is U4 and U6 that have the catalytic function I think.
what they dont tell you is that this is all happening at the same time of transcription... the 5 cap and the polyadenylation. 10/10 animation for beginners.
this is such a wonderful video! not only did it help me immediately understand the process but also made me super amazed by it! thank you!
I can't tell how helpful it was ✨ thanks a ton ❤️
Thanks for posting these vids! I've already watched 3 and can't stop!
Both of those terms refer to the nucleic acids (or nucleotides) that make up a strand of mRNA. The "A" refers to adenosine, and "Pyr-rich" refers to an area with a large quantity of pyrimidines. They act as signals/locations for parts of the process.
Too bad you didn't add the names of the different parts of the spliceosome
jyfdtrsextredckjhp9h
I'm 1 year too late, haha, but here you go:
The 5 snRNPs involved in splicing are U1, U2, U4, U5, and U6. They make up almost half the mass of the spliceosome. There are also other proteins which together with the snRPNPs make up the spliceosome.
hey hows it going im from he future how is it in 2014?
yeah appreciate
@@divelikejunk8557 NOICE
The best ever (?) animation on RNA splicing
splicesome are so kind.
These videos are brilliant!
I'm learning this stuff for the first time, but it's so damn interesting.
same bro so coooool
This really helped me a lot in my medical studies! Thanks! =D
this was so helpful, thank you very much
Still holds up, great quick video to show what's going on. Thanks!
The 5`-End (GU) of the Intron is fixing at the Branch Point (A).
Introns (INTervening RegiONS) are not coded Pieces of a RNA. At the Splicing, they will be depolymerised.
Exons (EXpressed regiONS) are the coded Pieces of the RNA, at the Splicing the Exon Parts will be fixed together.
prä-mRNA -> (Splicing) -> mature mRNA
There are also special Introns, that go in a enzymatic-conformation after Splicing.
Only Eukaryotes have Introns and Exons
Great for showing the bigger picture, thank you.
No way how smooth can this be omg wow
It's not correct right? Not all snRNP's stay with the spliceosome complex the whole proces, U1 and U4 should leave. Or am I wrong?
Simon Scholz You aren't, you are right. This video is imprecise, but still helps a lot imho
Rest in peace, intron🕊️🌹
"Greatest animations of all time
were all done 12, 13, 14 years ago"
Thank you for catching that--it's fixed!
I agree I learn more from you tube than I do @ florida College of Natural Health
Simply Brilliant
This is majestic.
It's god man
u r great. Would of been helpful if u included how the extron can also be furtherly spliced
@ilikecandycane It is a process that produces alternate mature mRNA's, in which some exons are actually removed along with introns. This leads to a large diversity of gene products from a single sequence. Usually, you would have binding inhibitors or even promoters attach to an end of an exon (splicing sequence) to either inhibit or promote splicing at that end (affinity to spliceosome). Check out the regulation of alternative splicing in the determination of sex of drosophila for an example.
This is beautiful thanks so much
The U subunits ARE there. They make up the spliceosome. In fact, the U1 and U4 subunits are supposed to dissociate before the lariat is formed. Of course, they didn't mention that, BUT they did show the subunit corresponding to U1 dissociate from the mRNA.
hi I was hoping you could help me, how does alternative splicing leads to a one amino acid change in protein sequence on the same gene, with all the exons being transcribed.
I SOOO AGREEE WITH YOU!!!! IT'S SO EASY TO STUDY NOW WITH ALL THESE FREAKING VIDEOS.
Wonderful video, thanks. I help me in me molecular biology class
Thank you very much for the nice animation. Very clear. Congratulation.
This video is very helpful. I love biochemistry! Woohooo!
Just amazing!!
This is a great video! It would only be better if you could name the snRNPs as they attach to the mRNA. Also, one inaccuracy is that the video doesn't show U1 and U4 leaving the complex after the spliceosome is activated for the following transesterifications.
Thank you :)
Fascinating
Dhanyawad (Thank You)
GOOD INITIAL STEP TO UNDERSTAND SPLICING
This video is very useful. Thanks a lot
I kinda like the weird/creepy music, lol.
very very helpful, thanks.
that is so beautiful.
Pretty sure I didn't need to know that for my exam, but that was cooooooool!!
What a great video!
@soccerchic315 alternative splicing. different exon combinations will form different proteins from the same pre-mRNA
LOL i'm glad because im in your position now...except im nearly at the end of revision and still have few weeks left before exam :D. so boys and girls! do not start to study a day or 2 days before exam
thanks a million! :D
It's the description that's wrong. The video is accurate.
VERY helpful..
thanks
i cried
that was beautiful *snif*
pff, the music is the best part
U1 binds to 5' then u2 binds to the A branch site , then the 5' binds to the A site with help of U1 and U4 and u6 after the binding u1 and u4 leave , then u5 binds to the 3' end and cuts it , then the exons binds and that's how it's done
and what will happen with the intron later?
Amazing❤️
hey thanx... nw i actually understood the actual process. it is so helpful.. this process i couldnt understood in todays class.. i do undestand nw....... thank u thank u so much. and plz............ do upload more such helpful video........:-)
Is GU and AG the only bases fond at the end of introns?
Does this mean the splice site is constant?
thanks........ but a A phosphodiester bond is actually formed between the 4' OH and the lactase phosphofructokinase end of the ligase ester. NT
amazing!!
is the purpose of this DNA compression? (encoding multiple proteins) I half expect to find a version of ZLib running in molecular clockwork..
Lovely!
Totally just said that in a Russian accent and it was amazing.
@ChickenWingChampion I guess not proteosome, since that's for protein... Most probably, RNAse enzyme is the one degrading the introns
wonderful!!!
this video helpd a lot......
Thanks but I have a question that why eukaryotes bears both exons and introns and not prokaryotes ???
cristian1092
they said in the video that it will be degraded
2:16
" the introns debranched, and is then degraded"
very cool. thank you
thank my brother
Yeah, that is what happens. And I don't know why people are getting thumbs down for pointing that out because it is true. I will likely get thumbs down too for saying that. In order for the lariat to be formed...U1 and U2 MUST leave the spliceosome.
how awesome... school needs to adapt and include links to videos for at home, then the classroom opens up for discussion
I'm looking for the 3' of the splice leader but can't identify the sequence. Any suggestions?
Hmmm... This isn't what I'm learning in my genetics class. We're being tuaght that 2'OH on the A site, with the help of splisomes that change the conformation of the mRNA, attacks the phosphodiester bond at the where the first exon and intron meet making the lariet. Next, again via conformation, the exposed 3'OH of the first exon attacks the phosphodiester bond at the remaining exon/intron connection. I guess I'll just go by what we learned in class.
just what i needed, thanx!!
is it a bird? is it a plane? NO! its DentistMan
Two transesterifications, between GU and A, and the cleaved 3' end of the exon and the AG.
Splicing occurs in the nucleus so that then mRNA only has exons when it leaves the nucleus to go get translated and what not
@soccerchic315 through the process of alternative splicing. a single primary transcript can be spliced into different mRNAs by the inclusion of different sets of exons.
amazing
its very clear thanks a lot
what are
"A" branch site
Pyr-rich region
?
or what are they doing ?
Thanks
Where in the does the splicing occur? Is it outside the nucleus?
No phosphodiester bond brakes during this... just transferring of bond takes place between exons unit.
thanks!
I stand corrected, "transesterification" is more accurate and precise.
fantastik mekanizma!
hey, uhm I hate tro bring it up, but how did you do?
nvm...this was intended for the transcription video
I wonder how exactly they visualised this or came up with the order of work of these proteins.
Great question
ELE VAI SER APROVEITADO PELA CELULA
video posted 15 years ago and here I am with a test on Friday lol
thanks
how is the intron degraded? Proteosome?
i wish my teacher knew how to teach like you
is this bio part 1 or 2?
@betteronblondes LMAO! Excellent reply.
There is a small problem here - the pre-mRNA is not cleaved. Chemically, splicing involves two phosphotransfer reactions, not cleavage followed by ligation as this video shows.
inside the nucleus, only when RNA is mature it's able to get out of the nucleus