Splicing

RNA Splicing | group 1 introns | group 2 introns | self splicing | spliceosome |
The transcription of eukaryotic gene results in formation of pre-mRNA. The pre mRNA have coding sequences and non coding sequences. The coding sequences are known as the exons, while the non coding sequence are known as the introns. splicing is a phenomonon in which the introns are removed and the exons are joined togeather to form mature mRNA. the basic mechanism of splicing is simple. the phosphodiester bond between exon and intron at 5' splice site is first broken, then the 3' hydroxyl group of the 5' exon, reacts with the 3' splice site to complete the reaction.

[@sarvsoltani1674]

it helped me in so many ways to better understanding how exactly the splicing takes place! thanks for the great job.

[@ummeabiha7323]

this is so far the most simplified yet the best video on the topic!!

[@CancerEnthusiast]

Loved it! I never understood splicing until now. Keep it up my friend, you have an excellent way of explaining.

[@ummulbanin9845]

Thank you sir for such a simple yet completely clear and understandable lecture

[@shamshassan6827]

thank you, you made such a complicated
process easy and clear to understand

[@SabrinaScience8]

Thank you so much for this video! I'm a visual and an auditory learner and I thought your explanation was perfect!

[@rachl8400]

Great video! Super helpful, one thing to note is I believe the correct naming of the G and A nucleosides is "guanosine" and "adenosine" because they are joined with ribose, not on their own.

[@yarelisrivas-avila7610]

Excellent explanation. Thank you so much!

[@apekshadandi7767]

It was quiet effortless to learn splicing from your video. Thanks a ton.

[@paige9274]

great video, explained very clearly thank you

[@rajuislam2490]

Thanks man.. I really get understand by your video a day before my exam.i cann't speak out how am i feeling now!!!!!!

[@KarthikDisley]

simplest and shortest explanation of all, very helpful

[@HadasLL]

I'm so happy I found you. You are great!

[@okankilic2297]

To add a minor revision, you could substitute "dash" with "prime" word. Thank you !

[@lorrainelolah6841]

hello Okan! can i kindly have any questions on pre- mRNA that you might be studying. i actually approached you since your comment is the most recent.

[@BioTechNomad]

😂😂😂😂 i was thinking the same

[@varnamohan2629]

We use prime

[@Sunshine-Muskan]

Great work 👍 very clearly explained

[@apurbamukherjee794]

It's very helpful, thank you for this video

[@joyganguly6834]

Really thank you for the video sir, I have never understood Splicing this good before

[@chloesmith2607]

Thanks man, I really appreciate this. Have a great day!

[@bracken7794]

Great explanation, thank you!

[@razanfeki2909]

you explained something in 7 minutes that my professor couldn't in and hour and a half !!!!

[@neethugopal2351]

Thank you.. its very helpful and easy to understand.

[@sharonbhambal2084]

Now I clearly understand this concept thanks

[@aaronmbwabi]

The best explanation ever!

[@linagabriel6356]

Thank you so the video.. It was very educating

[@ganeshgr616]

Great explanation..and . good vedio too.......

[@eisenj21188]

Great video buddy. Thanks!

[@md.ashrafulkarim6778]

very short and to the point..thank u

[@salmasanad710]

thaaabk you so much i spent three days trying to understand this, fortunately i found this and i understood it quicklyy so now i wish if i have founded this 3 days ago hahaha

[@pakeezahashraf197]

I did'nt understand my teacher's wording
But this vedio help me and make me to understand splicing easily
I just love thiss❤

[@yashusingh2289]

Really helpful..... thank U so much....keep it up......!!❤🤗

[@Reah850]

I know I will never ever e-ever give you uuup, and I wanna say thank you in case I don't thank you enouuugh...

[@lordhelmchen6539]

Very helpful. The simple explanation of attack here and there from my professor was not cutting it.

[@joshmemarrynagdey1639]

Helpful video. Thank you

[@mmmpr2261]

Bless you,man!saved my day'

[@memanjoo005]

Best video on splicing

[@drajiv1]

Nicely explained 👍

[@sujankumar3620]

Super explanation 👍

[@rajkishoredas936]

Thank you sir , the video was easy to understand .

[@anjanasaji6557]

Very nyz clz...easy to study😍❤️

[@user-me2uu4gy8w]

You are a god send. thank you.

[@the_explorer_anje8243]

Thanku so much for complete explanation

[@vaishallychugh8577]

Really very nice explaination

[@lucyxiong7301]

thanks for your help! you're pretty awesome!

[@akashsahu6856]

Thank you for your help 🙏🙏

[@danielolumese9083]

thanks making this video mate !

[@ayshamalik2963]

Much helpful💜💜💜

[@leyiwang3863]

Very helpful!!! Thank you!!

[@niks8924]

Very well explained sir

[@prastutidebi8034]

Really helpful thank you so much

[@Wilderness5283]

Thankk you, it is very informative!

[@mohanaburman8467]

Loved it ....

[@josephineobi8089]

Thank you for the video.
Please can you help with an explanation why in-vitro splicing reconstituted with U-snRNPs is slower than in-vivo splicing?
Thank you.

[@asmita_1999]

Great video

[@funnyvideomarwadi]

Nice information of RNA splicing

[@khoinguyen0210]

Thank you very easy to understand

[@user-mh3mn9ge2b]

You are great bro 👍

[@manjusamyuktha2013]

Good explanation thank you sir

[@shaharbanupp6975]

Tysm,, u really helped me a lot

[@mawadaa.nasser651]

Very simple, Thanks

[@user-tf2vh3jm1x]

1:50 ✍️ It helped me a lot, thx🙏

[@mobarkalwahishi3323]

Thank you so much

[@manikhalder3520]

loveeeeee you a lottttt god bless youuuuuu

[@valentinapaolillo6310]

thanks for the help, great video.

[@ahmadansari6829]

Great sir

[@bharatidas2108]

Thank you🙏

[@mitchellhayes2400]

Great video! What is your primary source for this video? Thanks.

[@240_mandakinisabar4]

Tq so much sir i really appreciate you ❤️

[@muhammadtouseeftahir9477]

thank you so much❣

[@Suche13]

thank you so much

[@arshbir1539]

Thank you

[@deebos980]

wow nice

[@mernas1418]

GOATED.

[@manibio2948]

Thanku so much ........

[@user8582]

AMAZING

[@silva0003]

Why are there no videos of examples using a template strand to help us identify introns and exons

[@durgamthirupathi1838]

I love you sir thanks

[@manikhalder3520]

you are gooooooood

[@karansahare1349]

Thanks sir

[@zoom-zip3473]

So where does the guanine nucleotide in type 1 introns come from?

[@beatriz4118]

Thanks

[@richardshane456]

ty

[@sumanpanigrahi7275]

Nice...

[@syafianursyifa5183]

excuse me, Mr. what's book you were used in this video ?

[@ishanbharti8797]

U5 is attached to both the exons, holding them together

[@extatickiddo5714]

I’ve never heard prime read as dash lol

[@gretabecker2946]

Me neither. Came to the comment section just to see if that's a thing... So, was this by accident or is it, indeed, called 5/3 dash in some other parts of the world?

[@shubhangimorale9684]

@@gretabecker2946 some people call it dash instead of prime which is conceptually wrong. It should always be 5’prime splice site.

[@nadiatumayine7341]

Thank you for letting me know that the dash meant prime, I was on my way to closing the video because I was even more confused :)

[@londonjeremiah8434]

instablaster...

[@bookishmahsa6634]

cool then I'm not alone

[@taniabhadra9313]

What happened to the introns after slicing?

[@HarshitKumarSinghAnine]

Thank❤❤❤❤❤❤

[@gdrt12385]

Sir which is best book for molecular biology study📚

[@alfredomunoz8791]

Also Prokaryotes can't have introns, because they have transcription coupled to translation. They don't have time/space for that, since intron splicing will stop the coupling. Eukaryotes evolved the nucleus, where splicing can be done

[@ganmeii8912]

they still can have introns, prokaryotes perform self splicing which means it won’t impact translation time wise but it can still happen

[@mashedtoilet3033]

Is "five dash" the correct pronunciation of 5' ? 10yrs ago when I had taken courses in biochemistry and ever since then I've only heard it as "five prime". your video is the first time I've ever heard of "five dash".

[@mimineelam1671]

so lariat forms only on group 2?

[@Alkimachos]

am I the only one who thinks snurp is the cutest name ever?

[@muhammadabdullah4121]

Why the splicing of intron in transcription occur in eukaryotic not in prokaryotes?

[@akashbaburaj2349]

can somebody explain what is branching sequence in mRNA and why it is called as branching sequence

[@subhasishbhattacharjee8596]

Thank you mam

[@Prettiest_Aayat]

Oh great

[@ankitcreations9678]

💓

[@alfredomunoz8791]

Thank you for 3:30 lariat

[@amnarehman1278]

Waaaaaaao 👍

[@user-sy6jx7cw7m]

То чувство, когда говоришь по-русски а слушаешь по-английски и все понимаешь)