Mycology Lab 101: Agar Work, Cloning, Spores & Sterile Culture Technique for Mushroom Cultivation

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  • Опубліковано 21 лис 2024

КОМЕНТАРІ • 323

  • @田中りょう-i9u
    @田中りょう-i9u 9 місяців тому +108

    Starting off felt like hitting a home run on my first bat, and now I’m stuck in a loop where progress seems like a myth. Watching this felt like someone’s live-streaming my current state of affairs-talk about relatable content! Your insights are the beacon of hope for my next endeavor. Thanks for shining a light on the path ahead!

    • @psilocyborg4775
      @psilocyborg4775 8 місяців тому +9

      Whenever I'm having trouble I just think to myself ' The more problems I have , the more I learn' That usually stops me from giving up👍

    • @davidlloyd3045
      @davidlloyd3045 8 місяців тому +2

      Mushroom is Mushroom you make it hard with big stupid words, you learn as you go that as all will realise love,peace powder and puff

    • @_-pf_gd-_
      @_-pf_gd-_ 4 місяці тому +4

      Stop hitting bats, animals have rights too.

    • @atitagain83
      @atitagain83 4 місяці тому

      Yeah, I got tired of fruiting nothing but whatever I breathed into the still box too.

    • @mikehulbert7343
      @mikehulbert7343 3 місяці тому

      ​@@davidlloyd3045 I laughed way more than I should have

  • @mavericks.9638
    @mavericks.9638 8 місяців тому +53

    this dude has a pure love for the science of mycology and its bio mechanical processes.

  • @Hairofthedog666
    @Hairofthedog666 9 місяців тому +68

    This is one of the best "101" videos I've seen on the platform. Well done!

    • @jeremyscloset
      @jeremyscloset Місяць тому

      Agreed. This is a great find today

  • @stevec8872
    @stevec8872 5 місяців тому +38

    Many spores of wisdom here. You are a golden teacher!

  • @uzzobb
    @uzzobb Місяць тому +2

    The Tao of Mushroom Growing. 02:10 It's no coincidence that he chose a river as a background. This guy gets not only mushrooms, but life. Thank you for the useful info and the beautiful way you share it with us!

  • @nerdymanjoe4948
    @nerdymanjoe4948 8 місяців тому +24

    I spent years doing sensitive wet work in a biology lab. Your advice for sterile work is better than anything I’ve seen before. I wish I had seen this video before having to learn things to hard way.

    • @SuperiorDave
      @SuperiorDave 18 днів тому

      Wet work! Like in Horrible Bosses?

  • @rainemincey9285
    @rainemincey9285 8 місяців тому +15

    This video is very much a super informative "bill nye the science guy" personality with much more inner dialogue that I go through. Couldn't have come at a better time

  • @TB-jl9fr
    @TB-jl9fr Місяць тому +1

    Yeah i remember when i started with growing mushrooms. Damn it was a lot of thingso learn, and back then (almost 11 years) there wasn't any helpful stuff like this guy on YT.
    Most of my first approaches failed so hard, but when you get your first succesfull growth it's a self-runner.

  • @briand.4398
    @briand.4398 5 місяців тому +15

    Good job young man. I have ADHD but was enthralled enough to stay focused till the end. Amazing feat if you knew me. :) Kind of the reason I am exploring medicinal benefits of mushrooms.

  • @chadcowan6912
    @chadcowan6912 6 місяців тому +14

    As a beginner to mycology, I just wanted to say that your videos are the most approachable while being coherently technical. Your production quality and sense of humor are a welcome addition.
    I can see that you are all-in with this endeavor! The 1025R tractor and beautiful lab are clear signs of your investment. Good luck, and thank you for the guidance 🙏

  • @skullcrushers1000
    @skullcrushers1000 2 місяці тому +2

    You are a godsend. These high-quality, long-form videos are so much more helpful than reading through pages of Shroomery from decades ago. And you keep it entertaining too!
    I wish you the best of luck. You deserve all the success.

  • @mikexibalbafarms4169
    @mikexibalbafarms4169 9 місяців тому +12

    Fungaia makes thee best cultivation videos out there!🙌🔥🔥🔥🔥🔥🙏💚

  • @himawari9430
    @himawari9430 9 місяців тому +11

    this is my favorite youtube channel ever in the whole world

  • @audreycotten1638
    @audreycotten1638 9 місяців тому +12

    Standing by ready to soak up the wisdom! Your presentations are the most informative on UA-cam and your slightly goofy personality make science class fun! Thank you for sharing your spore wisdom with us❤

  • @Mohawk763
    @Mohawk763 7 місяців тому +4

    Absolutely excellent information. I was wondering why my plates contaminated at the edges, and figured 'bad plate' from supplier....then I saw your technique with micro-pore and learned I might be the problem. And that's a morsel of the information I've taken from this video! Thank you.

  • @Perryloc
    @Perryloc 9 місяців тому +7

    Nice surprise for Thursday night viewing. Thank you for sharing knowledge, your time, and effort. It is greatly appreciated!

  • @aj9485
    @aj9485 7 місяців тому +3

    Pure example of dedication to science. Perfect!

  • @aidennymes6335
    @aidennymes6335 9 місяців тому +12

    hope your channel blows up and you get all the attention you deserve..thanks for your contribution to the community

  • @s0cializedpsych0path
    @s0cializedpsych0path 2 дні тому

    This is the best mushroom intro video I've ever seen.... posting on MycoMafia!

  • @fungifocus
    @fungifocus 8 місяців тому +4

    this is a must watch for every level of mycologist

  • @mawman9976
    @mawman9976 9 місяців тому +4

    ❤❤❤
    Yes all videos from you are top.
    I'm having to get involved with mycology to treat my stubborn chronic cluster headache.
    Really appreciate such wisdom ❤❤❤❤

  • @Omoschedastico
    @Omoschedastico 8 місяців тому +4

    Thank you fungaia for your passion and for all the information you are giving us for free!

  • @fractalbroccoli
    @fractalbroccoli 8 місяців тому +3

    By far the most informative fungi cultivation vid I have ever seen. I’m really stoked and I’m gonna give this cultivation a go real soon. Thanks for sharing your knowledge and keeping the vid interesting and comedic.

  • @nastydave6334
    @nastydave6334 7 місяців тому +1

    Learning on UA-cam has been a challenge. A lot of the growers are also selling mentorships and consults. It seems like much of their content is just feeding you enough information to get you interested in a paid mentorship (nothing wrong with that). This video is the first one that I found that went into detail and specific processes in this much depth. This is after watching dozens of hours of content from other channels.

  • @audreycotten1638
    @audreycotten1638 9 місяців тому +6

    New to growing shrooms and LOVE your channel! Greetings with Much Enthusiasm from WA state also🍄❤️

  • @JO-br8tn
    @JO-br8tn 8 місяців тому +2

    Bro. One of the best videos on youtube for mushrooms. Your speaking my language. Keep em coming

  • @migotkami2341
    @migotkami2341 8 місяців тому +1

    Wow 🤯Wow, the best video I've ever seen, the most knowledge presented concisely and simply, since I fell in love with this topic. That's over a year ago spending hours on watching different stuff on UA-cam and other places.

  • @drstraange1
    @drstraange1 9 місяців тому +10

    Thanks dude for another great video!!

  • @dcfromthev
    @dcfromthev 9 місяців тому +5

    This channel is off the chain!! Please keep the videos coming, I love your work!

  • @paulrennie3819
    @paulrennie3819 7 місяців тому +2

    Excellent info. Struggled with using parafilm myself before. All these tips and tricks which come from so many hours of work is going to help me next time.
    Going to save this video for future reference also.
    Thanks so much

  • @MrOldclunker
    @MrOldclunker 8 місяців тому +12

    I work in a micro lab and a small flame of an alcohol burner is not going to give you a small sterile area around the burner. In fact any upward movement of air by thermal rise is just going to bring the particles into your area faster from lower in the air column.

    • @dheasley2
      @dheasley2 8 місяців тому +2

      Because in that small area of upward rise isn't enough heat to kill anything that's already sanitized? Have you heard of oven tech for in the home?

    • @bully1676
      @bully1676 3 місяці тому +1

      What technique did they use before electricity?

    • @tylerdelong5508
      @tylerdelong5508 2 дні тому

      Ima para pharase for the creator.... Keep in mind the flame only works in a giant sab. Lol. But the sab is a placebo not your alcohol flame.

  • @WheatSupreme
    @WheatSupreme 9 місяців тому +3

    Amazing content. I could have watched for several more hours.

  • @Naturamorpho
    @Naturamorpho 5 місяців тому +1

    Waw! So much information in a single video! I don't do mushroom culture (although I may, eventually, who knows...), I do Orchid Seed In Vitro culture. Still, so much of this information was useful and relevant for my own techniques!! I mostly try to avoid the fungi, bacteria and other contamination and I use a glovebox. A home made glovebox! It takes a whole lot of confidence to do it just next to a flame and no walls around! Thank you for the insightful content!

  • @catscradletextiles1400
    @catscradletextiles1400 9 місяців тому +3

    This was incredible, thank you! I wonder if you have any knowledge of folks cultivating mushrooms for dyeing textiles?

    • @_fungaia
      @_fungaia  8 місяців тому +1

      I know a lot of folks use mushrooms for natural dyes, but mostly just foraged specimens. Haven't come across cultivators specifically growing for that purpose, but no reason not to try it!

  • @syberphish
    @syberphish 9 місяців тому +5

    Wow! What an incredible video, you cover all the bases without being high af or talking through a mask. 😄Excellent content! I absolutely despise the way scalpel blades attach to the handle. Can't believe no one has come up with a better solution yet.

    • @dcfromthev
      @dcfromthev 9 місяців тому +3

      I totally agree about the blades, it’s setting us up for injuries! I think I’m going to start using pliers to hold the blade when changing them out, for added safety.

    • @syberphish
      @syberphish 9 місяців тому

      @@dcfromthev Thank you! For some dumb reason that thought never even occurred to me. I'm legally blind, can only see partially out one eye and I always feel like I'm about to stab myself while changing them. Very tedious.
      No joke, when I opened the handle the first time, I thought it was defective. I was like... this is IT?? You've got to be kidding me. Seems like a design that hasn't changed in the last 600 years or so, it just seems incredibly unsafe. (even for people who can see just fine)

    • @_fungaia
      @_fungaia  8 місяців тому +3

      Haha I actually stabbed myself while I was putting the blade on for that clip in the video

    • @syberphish
      @syberphish 8 місяців тому +1

      @@_fungaia Wasn't sure if I should like that or not but it sounds like you understand me all too well. 😄

    • @syberphish
      @syberphish 8 місяців тому

      @@dcfromthev bro, does your name mean what i think it means?

  • @hilohahoma4107
    @hilohahoma4107 3 місяці тому +1

    Cheers to this fungifile and cheers to myself for watching from start to finish, great knowledge and subscribed ❤😊

  • @m.i.c.h.o
    @m.i.c.h.o 8 місяців тому +2

    Aw man you're just like me! Except you know way more and have way more experience in mycology. Thank you for this banger video, I'll be learning from this for a very long time. And I know this is late, but welcome back to youtube!

  • @houssembentounsi5184
    @houssembentounsi5184 6 місяців тому

    I am a mycologist myself and i learned a whole new approach of my discipline with you thank you

  • @vhmccray3705
    @vhmccray3705 7 місяців тому +1

    Simply awesome! Like you said... a lot of info. But one thing I was wondering; do you soak the grain before inoculation? Or just let the mycelium devour the sterile grain? It took me two days to get all the way through, but it was worth it. Keep up the good work. I look forward to checking out more of your videos. Oh, I just noticed a deep dive on how to make grain spawn 😊 Guess that's my next stop!

  • @jennyloohoo
    @jennyloohoo 7 місяців тому +1

    This was very useful info, just now starting agar work.

  • @SarahDibie
    @SarahDibie Місяць тому

    Watching this felt like someone’s live-streaming my current state of affairs-talk about relatable content!

  • @ten-hx2xi
    @ten-hx2xi 7 місяців тому +2

    thank you, not only for this great video, but also for clearly demonstrating what all good teachers know, Entertainment makes for great education! ❤

  • @EsotericMajestic420
    @EsotericMajestic420 9 місяців тому +7

    Liked and subscribed.
    Thanks for teaching me

  • @jameswheatley9883
    @jameswheatley9883 7 місяців тому +1

    Ran some true blue genetics in some all in one bags a few months ago, the local shop here in Denver sells their stuff! Extremely entertaining and educational video loved it thank you very much!🍄🌱😎

  • @ZehMariap
    @ZehMariap 2 місяці тому +1

    That was a long instruction, I've loved each second. I would like a sample of Felinus Robiniae.

  • @aarondingus3548
    @aarondingus3548 9 місяців тому +2

    Great video, thanks for spreading spores of knowledge 🍄

  • @christianblocker1782
    @christianblocker1782 8 місяців тому +1

    Waiting on my first lion's mane grow kit right now but I'm already eyeing a more complex indoor mushroom growing setup, thank you for the video

  • @jonahmartin332
    @jonahmartin332 11 днів тому

    I’ve been studying this videos for a few days now. So much info, definitely have a notebook handy. Thank you for your enthusiasm, and information

  • @JohnSmith-us2ru
    @JohnSmith-us2ru 8 місяців тому +2

    This video was educational and made the process seem less intimidating.

  • @blur3d
    @blur3d 5 місяців тому +1

    Really enjoyable and amazing resource for mycology. Thank you!

  • @thraxxius
    @thraxxius 19 днів тому

    I love it! Your videos are so educational in a fun and professionally way. Thanks to you I will sterilise my old syringe and start cultivating again

  • @oouziii4679
    @oouziii4679 4 місяці тому

    Your natural poeticism and enthusiasm for nature's beauty is inspiring and refreshing

  • @wombatkins
    @wombatkins 3 місяці тому +1

    Im learning so much that even if i fail my first grow im still having fun

  • @akdrywallguy60
    @akdrywallguy60 9 місяців тому +1

    Wow! The timing of your video drop is perfect for my level of knowledge right now! I've been playing around with agar with some successes and a few failures as well. The techniques that you've outlined are EXACTLY what I need right now! Looking forward to taking my agar genetics back to LC and onto grain. Thanks again for your amazing content! I'm going to go check out your website now. Mush love!

  • @clementnataf9520
    @clementnataf9520 8 місяців тому +1

    Bro I love your videos, I watch tones of videos and following a couple of the best channels, you deserve a 5 stars for the way you educate.
    Love you man and love gaïa.

  • @Nokaramu
    @Nokaramu 8 місяців тому +1

    Damn man this was really a great video! I just started today working with agar and I'm super excited to keep expanding my shroom repertory. Thank you for this information that is truly PURE GOLD!

  • @GeneralMcNuggs
    @GeneralMcNuggs 5 місяців тому +1

    Amazing knowledgeable job Sir.

  • @mateo8120
    @mateo8120 2 місяці тому

    Such a good video. I can feel the passion for mycology through the screen. This is what I love, Good people sharing their knowledge in a creative way for all of us to learn. Well done, and thanks for sharing with us!

  • @danielmcardle3476
    @danielmcardle3476 17 днів тому

    I really enjoy using clingwrap on petri dishes! I find it simple, effective and immensely satisfying. Cheaper and easier than parafilm.

  • @ambrosioplaza3899
    @ambrosioplaza3899 7 місяців тому +1

    Hi from Caracas Venezuela, excellent content my friend... I learned a lot in this first video I have seen.... I am just starting... One of the best educational content if not the best I have found on UA-cam, to the point and cristal clear 👏👏👏

  • @nathanarcher6764
    @nathanarcher6764 5 місяців тому +2

    God Bless You, your motivation is much needed. Mycology is like trying to take on the entire royal navy and you're jack sparrow, the rum is always gone, and your compass doesn't point north; only at what you most desire.

    • @pennymiller2254
      @pennymiller2254 4 місяці тому

      Do you want me to take a lesson from Jack sparrow ;-)

  • @themushroombloke
    @themushroombloke 9 місяців тому +2

    One thing that I can add as a possible improvement is the use of grafting tape instead of parafilm. Cheaper, easier and mycelium doesn't need to breathe.

    • @_fungaia
      @_fungaia  8 місяців тому +1

      Great tip!

  • @Eric-w8l
    @Eric-w8l 2 місяці тому

    I just found your channel and I have to say that you are extremely underrated. Keep up the great work my friend, I'm sure everyone else enjoys your positive attitude as well! Many thanks.

  • @kiliankilian8747
    @kiliankilian8747 8 місяців тому +1

    thank you ! im waiting for your next video about maintaining a mushroom culture libary!!

  • @themushroombloke
    @themushroombloke 9 місяців тому +9

    This video was awesome. Well explained. Well shot. Perfect amount of dorky cringe.Thank you!

    • @jennak.8541
      @jennak.8541 8 місяців тому +2

      I agree with you. Just the right amount.

  • @MacCelium
    @MacCelium 8 місяців тому

    Nice contribution to the growing community. Thanks! Pro tip...bag the Parafilm and just use grafting tape. Dirt cheap and easier to work with. A twist or 2 before you break it will make the ends easy to find.

  • @jake_runs_the_world
    @jake_runs_the_world 7 місяців тому +1

    goldmine for me whos trying to get my mushroom business up and running !

  • @Sturdy_Penguin
    @Sturdy_Penguin Місяць тому

    Damn, this was a great video. I majored in biology with a focus on biotechnology so I got to learn all about sterile technique and am usually super disappointed with how people in tutorials for things like canning food or growing mushrooms don't do a great job of sanitization let alone sterilization or know the difference, but this was on par with taking a microbiology course. I did a lot of tissue culture, so I'm familiar with having to tell people to open the door in the culture room slowly to avoid airflow disruptions and having the words "mycoplasma contamination" trigger Vietnam flashbacks. This is especially true considering you don't have the many-thousand dollar pieces of research lab equipment with a sliding sash, programmable UV sterilization cycles, and gas or vacuum line hookups to work with. Also taking into account different levels of access to supplies for people who follow your tutorials.

  • @panfabn
    @panfabn Місяць тому +1

    Worth a subscription for me.

  • @DS-xg9kf
    @DS-xg9kf 9 місяців тому +6

    A fantastic video. Thank you.

  • @kylegivler8372
    @kylegivler8372 6 місяців тому +1

    Thanks for sharing your knowledge :)

  • @gabrielwong6198
    @gabrielwong6198 8 місяців тому +1

    Amazing life advice on not giving up. Thank you for that !

  • @picklepie781
    @picklepie781 8 місяців тому +1

    Can't believe this is free 😮thank you very much

  • @TommyMcSharp
    @TommyMcSharp 5 місяців тому +1

    It's probably the most informative video about this subject I've watched over the last few years. Well done! Keep up the good work 👏 looking forward to more videos! Definitely like and subscribe 💪

  • @Shadow.strain
    @Shadow.strain 2 місяці тому

    You put so much effort in this video I would say that this is the ultimate beginner guide to mycology 👊🏻💯 thank you for sharing your insights and knowledge on this journey through the world of fungi 👍🏻

  • @pwnzormcduck6546
    @pwnzormcduck6546 8 днів тому

    This guy is awesome. He teaches it all but the mushroom community as a whole is great. They always offer help and information. Hey man I have a envirco Mac 10 XL 2*4 flow hood. It is my first hood, how do you recommend cleaning the face of it. I got it refurbished from nothing but air. They are highly rated and known in the community. Has brand new filters and was cleaned. I just want to know how I should clean the grill of it. I know you don't want the filter material getting wet.

  • @FainTMako
    @FainTMako 14 днів тому

    love your guide and I was really surprised by the amount of knowledge you have. Its obvious that you not only have done your research but you are passionate about the craft and you clearly have experience :)
    One thing I want to add as my personal opinion though is that with petri dishes its better to not let the mycelium grow to the outer edges. If I notice that my plates are almost fully colonized I will try to just use them to inoculate some bags or something but I wouldnt use them for more lab work.

    • @_fungaia
      @_fungaia  8 днів тому +1

      Appreciate the kind words! I'll pass the tip on up, I haven't heard of that before. Sounds worth looking into.

  • @DaysenSouth-z5p
    @DaysenSouth-z5p 6 місяців тому +1

    Bro dropping knowledge gold

  • @mattharvey8712
    @mattharvey8712 9 місяців тому +4

    Bravo.....mom would say.......teach them to fish.......great share......have u tried black light on ur grow........cheers

  • @Microbe_Master
    @Microbe_Master 7 місяців тому +1

    Thank you for spreading this information to all that may see it

  • @audreycotten1638
    @audreycotten1638 9 місяців тому +4

    Well Done, Sir and Bravo!

  • @cryptohound
    @cryptohound 2 місяці тому

    You have a relatable teaching style and your passion for mycology shines bro. Ty subbed

  • @Jeremy-i9f
    @Jeremy-i9f 7 місяців тому +1

    This was a seriously good video.

  • @imalex1239
    @imalex1239 3 місяці тому

    Man you are a living Golden Teacher! Thabks for your dedication ❤

  • @ditz2998
    @ditz2998 Місяць тому +1

    The alcohol you use in the oil burner isn't sold in the UK. What other clean burning liquids could I burn instead?

  • @TerpyTen
    @TerpyTen 4 місяці тому

    I always choose the flail method. I have about 150lbs of spawn and substrate all ready. The Enoki are ready for fruiting. Right behind them are the 4 varieties of oysters. Mushrooms are fun. I wish i had the space for a laminar flow hood. I'm doing everything in a still air box or open room.

  • @madpenguin0515
    @madpenguin0515 8 місяців тому +1

    Holy shmoly!!! What an amazing collection of information, tips and tricks all organized and easy to digest! Looking forward yo getting started on my new adventure, and more of these! Thank you!

  • @peabea1
    @peabea1 4 місяці тому

    this is wonderful content! hands down best "mycology 101" on youtube. edit quality and informitive on par with fresh from farm Gary. CHEERS

  • @jeremyclimbs
    @jeremyclimbs 8 місяців тому +1

    Can you make a video on identifying what is bacterial growth vs mycelium?

    • @_fungaia
      @_fungaia  8 місяців тому +1

      Yes, keep an eye out for a whole video on contamination!

  • @Michael-x9j7b
    @Michael-x9j7b 3 місяці тому

    Man, I'm so glad I found this channel, thank you thank you thank you.😃

  • @landryquyenhouizot9792
    @landryquyenhouizot9792 8 місяців тому +1

    Salut, j'aime beaucoup votre vidéo c'est très instructif cela va beaucoup m'aider pour mon futur métier, à bientôt.

  • @NihilisticRealism
    @NihilisticRealism 7 місяців тому +1

    You are great. keep this up.
    thank you for your efforts

  • @SubconsciousLight
    @SubconsciousLight 7 місяців тому

    47:04 Omg they evolving! He escaped and were even that sneaky that no one noticed!
    But for real, can´t wait when we use mycelium full potential and start to GROW natural biological stuff out of them! Even connect them to technology! It is already happening on low lvl! Making music with them, out of their impulsing signals, plastic eating bacteria, mushroom hat, mycelium motherboards and labyrint solving mushrooms! We are getting there!
    Great video, thank you!

  • @systemic_disclosure766
    @systemic_disclosure766 7 місяців тому +2

    The professer in the lab coat looks just like Hamilton Morris! (my fav chemist)

  • @satxs5974
    @satxs5974 8 місяців тому +1

    This was an exceptionally well made video. Subscribed and looking forward to more great content.

  • @sonnybrasco9735
    @sonnybrasco9735 9 місяців тому +5

    Thank you for this 💙

  • @VertigoVenturer
    @VertigoVenturer 8 місяців тому +1

    the enthusiasm is contagious! mush love

  • @EVRGRN
    @EVRGRN 3 місяці тому

    35:52 I’ve gotten the most value out of your videos than anywhere else. Thanks for being the Bill Nye of mushrooms! Any hygrometer you’d recommend? I keep getting varying humidities between hygrometers.

  • @digitalphanes206
    @digitalphanes206 6 місяців тому +1

    what a great videos, thanks

  • @Xulzen
    @Xulzen 6 місяців тому

    Just here to show appreciation for your work and to help with the algorithm.