Such a wonderful video! I absolutely love the advice. Would this sterilisation method for the nepenthes also work for staghorn fern spores by any chance?
Your channel is great! I've been wanting to get into tissue culture so badly. I'm obsessed with plants, not just house plants but every little thing growing. We went to visit my wife's family in Philippines and I spent a ton of time just looking at plants. I'm gathering lab equipment, but I need a laminar flow hood. Im going to build my own, and I am debating on adding a bottom floor with holes, and an attached fan chamber to pull vapors down and out of the house through a vent. Are there any chemicals that have dangerous vapors in tissue culture, to warrant my concern? Or, would I be better off not adding the suction. I could see where the exhaust vent could suck contaminated air from the window that you put your hands through and into the sterile area of the workspace.
I love your videos, i am a fellow plant and fungus lover. I culture fungi using some similar methods and i am moving back into plant tissue culture. It is amazing all the things we can do with agar and different types of sugars. Have you guys tried light malt extract for the sugar source? How about potato or rice water? Im cery interested to know
Love the channel. Learned so much and had some nice results. However, I don't have time to keep up with the hobby for now. I have a set of biocouplers (never used). I am in the dmv area. Can I donate them for someone wanting to get into tissue culture? Love what you guys are doing, would like to give back.
Thanks so much!! It's great what you want to do. Where do you live? We will communicate with our marketing team to see if we have any enthusiasts who are limited by budget but eagerly want to try tissue culture. We can help them with this donation of yours. How does that sound to you?
I have been wondering this for a week or so, but would it be possible to sterilize the plants in a Sodium dicloro solution put in a vacuum chamber for a short period of time? or would the water in the plant just start boiling and kill the plant instantly? I thinking you don't even need full vacuum, just enough to break the surface tension. I know tween-20 is used for the surfactant but i was thinking a vacuum chamber would help also with getting the sanitizer into every crack and crevasse of the plant. food for thought I guess...
What species have you been able to get into tc from tissue? I heard that nepenthes, like the highlanders, have an internal bacteria that make them hard to place into tc from tissue. Also would they have to have a nighttime temp drip while in tc?
@@kasunrphoto You can do tissue culture at home. But, you need to have setup, clean environment, and a working protocol for the plant you want to grow in vitro.
I've been watching you a week and already have things ordered to get started. However, I ordered a cannabis starter kit, as it was $99, and that was all I saw. Can I use that to get started with other plants, then move them over once the other media is here? I live in a rv now, planning a homestead. Do you have any advice on keeping plants around for a long time?
@PlantCellTechnology it has a complete cannabis cloning mix, and multiplication mix. I've been looking at your channel, and I've come up with yes. I have other media materials on the way. Got a little over zealous and ordered plants too. I was checking in case anything came early or with issues.
@@FarmerShawns This is great! You seem super excited about your experiments.🤩🤩 We hope they turn out great. In any condition, do not neglect to maintain an aseptic environment for your cultures. The health of your plants depends on that, too. And, if you need any help, let us know!
Started crying at 14:35, but so happy you two made this video! I love both your channels and PCT store!
We are in awe! Thanks so much for your love and support!
Cutting the plants always tuggs at our hearts, but the good news is, you will get many more after tissue culture! Thank you for the kind words!
How much sugar would you recommend for a 20ml solution of diluted hydrogren peroxide? for helping with seeds germination
Such a wonderful video! I absolutely love the advice. Would this sterilisation method for the nepenthes also work for staghorn fern spores by any chance?
Thank you. Great video!
Thanks. Happy to know that you find it useful!
Your channel is great! I've been wanting to get into tissue culture so badly. I'm obsessed with plants, not just house plants but every little thing growing. We went to visit my wife's family in Philippines and I spent a ton of time just looking at plants. I'm gathering lab equipment, but I need a laminar flow hood. Im going to build my own, and I am debating on adding a bottom floor with holes, and an attached fan chamber to pull vapors down and out of the house through a vent. Are there any chemicals that have dangerous vapors in tissue culture, to warrant my concern? Or, would I be better off not adding the suction. I could see where the exhaust vent could suck contaminated air from the window that you put your hands through and into the sterile area of the workspace.
Why don't you give a try to our convenient portable laminar flow hood instead?www.plantcelltechnology.com/the-mini-portable-laminar-flow-hood/
Great Job Thank you both.
Thanks!!
I love your videos, i am a fellow plant and fungus lover. I culture fungi using some similar methods and i am moving back into plant tissue culture. It is amazing all the things we can do with agar and different types of sugars. Have you guys tried light malt extract for the sugar source? How about potato or rice water? Im cery interested to know
Love the channel. Learned so much and had some nice results. However, I don't have time to keep up with the hobby for now. I have a set of biocouplers (never used). I am in the dmv area. Can I donate them for someone wanting to get into tissue culture? Love what you guys are doing, would like to give back.
Thanks so much!! It's great what you want to do. Where do you live? We will communicate with our marketing team to see if we have any enthusiasts who are limited by budget but eagerly want to try tissue culture. We can help them with this donation of yours. How does that sound to you?
I have been wondering this for a week or so, but would it be possible to sterilize the plants in a Sodium dicloro solution put in a vacuum chamber for a short period of time? or would the water in the plant just start boiling and kill the plant instantly? I thinking you don't even need full vacuum, just enough to break the surface tension. I know tween-20 is used for the surfactant but i was thinking a vacuum chamber would help also with getting the sanitizer into every crack and crevasse of the plant. food for thought I guess...
This is a video I’ve been wanting for a long time 😁
We were thrilled to finally add it to the channel!
What species have you been able to get into tc from tissue? I heard that nepenthes, like the highlanders, have an internal bacteria that make them hard to place into tc from tissue. Also would they have to have a nighttime temp drip while in tc?
Thanks, realmente great.
Nice video. where do you located? I want to get some information on tissue culture.
We are located in Washington DC. What do you want to know about TC?
@@PlantCellTechnology want to get information on if we can do tissue culture for curry leaf plants at home?
@@kasunrphoto You can do tissue culture at home. But, you need to have setup, clean environment, and a working protocol for the plant you want to grow in vitro.
Great video. how long for sterilization seeds with hydrogen peroxide? Thank you
24 hours was enough.
@@PlantCellTechnology thank you very much sir.
Hi , so if i understand , no hormone in the seed media ?
yes, right.
I've been watching you a week and already have things ordered to get started. However, I ordered a cannabis starter kit, as it was $99, and that was all I saw. Can I use that to get started with other plants, then move them over once the other media is here? I live in a rv now, planning a homestead. Do you have any advice on keeping plants around for a long time?
Hi,
Great to hear from you!
Before we suggest anything, can you tell what the kit actually contains?
@PlantCellTechnology it has a complete cannabis cloning mix, and multiplication mix. I've been looking at your channel, and I've come up with yes. I have other media materials on the way. Got a little over zealous and ordered plants too. I was checking in case anything came early or with issues.
@@FarmerShawns This is great! You seem super excited about your experiments.🤩🤩 We hope they turn out great. In any condition, do not neglect to maintain an aseptic environment for your cultures. The health of your plants depends on that, too. And, if you need any help, let us know!
Can another cytokinin be used in place of TDZ?
Francisco uses TDZ for callus induction.
How can I dispose of the tc media?
Thanks a lot and please update results! I hope they will be all successful👍🙏
Absolutely! Thanks for your love and support!
🌿🍀Thanks♡🙏🏽☘
Happy to help!
I need update on this
Sure!
I need a woman that looks at me, like she does at Francisco :D
HaHA! Laur is an excellent tissue culturist herself, and we really enjoyed making this video with her.