GLUCOSE MEASUREMENT
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- Опубліковано 18 вер 2024
- Related video 👉 • CARBOHYDRATES
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Clear + concise= excellent lecture 🙂❤️❤️❤️
Makes my heart melt. Thank you so much ♥️♥️♥️♥️
wow. that was very good presentation! I'm happy I found this channel. you explain it in a simple and comprehensive way. Watching from New York, Cubao!
abna hahaha
Awesome and very educative. I enjoyed every bit of it. Thank you.
Please I would like to know if the Benedict's test can also determine the concentration of glucose in a sample and how to go about it. Many thanks.
Hi. I just saw your comment.
Clinitest (Benedict’s test) is a copper reduction test that can be used to test for glucose but is usually used to screen for other reducing substances which may be present. But Benedict's has low sensitivity, so in the lab, we usually use reagent strips that are impregnated with the enzyme Glucose oxidase and this reagent strip detects only Glucose. We can also evaluate the condition based on the results of two tests (Benedicts and Rgt strips).
For example:
If + for Benedicts and + for Rgt strip = this could mean the presence of glucose and other reducing substances.
If + for Benedicts and negative for Rgt strip = this could mean non-glucose reducing substance or could also be interference from ascorbic acid.
If negative for Benedicts and positive for Rgt strip = this could mean a small amount of glucose is present in the sample.
@@MsLabCuison Thank you so much.
@@izuchukwuiwuamadi7039 You are welcome. Have a great day!
The best presentation. Proud student here.
Thank you, Marv :)
THANK YOU SO MUCH
Hello, do you have any pdf version of this lecture ? Thank you.
Hello! Please send me an email so I can provide instructions on access to these materials.
Here’s my email:
ms.lab0517@gmail.com
Hay so timely, diri nlng ko mereview hahaha
Hahahaha Gosh, indi ko late!!! 🤣
Nice.... Important... Go ahead
Thank you :)
What is the effect of a hemolyzed sample on the result of glucose measurement using the oxidase method? Can you answer it??
Hi, Ftoun 3! :)
Normally, when there is hemolysis, the blood glucose is decreased because of “dilutional effect” due to the release of intracellular components to the extracellular fluid (e.g. plasma).
In the glucose oxidase method, I think the release of hemoglobin from the lysed cells will decrease the glucose values. Since there is an increased amount of hemoglobin released from the cells, this hemoglobin will be oxidized by the enzyme GOD (so the amount of glucose that is oxidized is relatively decreased). Also, in the spectrophotometric method, the presence of hemoglobin and other reducing agents like uric acid and bilirubin (end product of hemoglobin degradation) will compete with the coloring agent that is usually used in the analysis, causing falsely decreased values. Also, these substances are oxidized by peroxidase and will decrease the amount of chromogen that is oxidized.
Just remember these:
• In the polarographic method, the level of oxygen depletion is measured and is proportional to the amount of glucose present.
• In Spectrophotometric measurement, oxidized chromogen is proportional to the amount of glucose present.
I hope I clarified things well 😊
We need lecture about monohexse pathway
I will try to upload a video on that 😊 Just kinda busy right now
👍❤️