Thanks for watching! Get the full article here: learn.freshcap.com/growing/agar-agar-so-nice-they-named-it-twice-5-steps-to-pouring-perfect-plates/ Have FUN growing mushrooms :)
Best editing and fastest presentation of information I've ever seen on UA-cam. You are a true artist, Tony. "Trifles make perfection, and perfection is no trifle. Genius is eternal patience." - Michelangelo Buonarroti
I personally think this is second best to Mark Keith's tequnique. He just missed a couple of important notes and tips. Agar solidifies at around 120°f and pouring as cool as you can reduces condensation. I'm also not partial to how he handles the dishes after they have cooled. The insides can be assumed sterile but to really go the extra mile you could place them in a sterile container before shutting the flow hood off. You likely aren't going to pressure cook a tote to put your petri dishes in, and sanitizing a tote is second best. So where do you find a sterile container suitable? The bag they came in is sterile, so if you roll it up and place it under your rack, once your plates are set you can put them right back in the bag, then you dont need to waste the parafilm and dont risk drying out your media. Is this technique straightforward and to the point? Yes. It's very professionally done in both style and production. Please take this as constructive criticism, I'm not bashing at all.
@@FreshCapMushrooms I was wondering if you would be able to do a video on how to spec out and make a 24x24 flow hood and maybe a parts list. I know you did a video on making a 24x48 flow hood but I just think that's to big and expensive for the stage that I am at right now. Side note, I live your videos, the first one I seen was the bucket tek on and that just got me so interested in growing mushrooms. Thank you again for all the info and videos you do. Keep up the amazing work. I don't know if you coild maybe email me the instructions if you don't want to make a video. I'm finding so much information on UA-cam and it is kind of overwhelming and I don't know who to believe and follow hence why I'm asking you I feel that the knowledge that you have in the videos that you have done so far have been an amazing help to me so far
dude, where have you been all my life? i've been learning mycology for a while now and had so many simple yet significant questions that all other online sources have failed to answer until now. thank you and keep up the good work! i'm so happy to find someone willing and capable to include the attention to detail that is so necessary to succeeding in this field. i will be recommending your videos to all my noob myco peers, and hopefully before too long i will have developed the skills and confidence to share my own wisdom via quality video tutorials like these. respect :)
I have an exam tomorrow on Mushroom cultivation technology and I can't explain how helpful your videos are proving to me. Your explanations and physical demonstration are so easy to understand. My book is really annoying and I was having a hard time preparing for tomorrow so thank you for such amazing content. And writing this currently, I have already watched 10 of your videos.
You are so wonderfully informative. By teaching the ignorant like myself, you are changing lives. It takes a community to save the world, a great start.
Great Video! As a passionate Home Brewer I can tell you that you‘ll find all the materials in good brew stores. Including petri dishes Agar agar and off course the rye which is used in the mason jars. These materials are also used for yeast cultivation.
I used a pressure cooker for the first time today. I also made agar dishes and innoculated them for thr first time. First grow ever actually, hoping it goes well but if not thats why i chose to learn the agar! Thanks !
@markgarry7866 by now amazing man. Currently I have 3 LC's I work with TAT, PE, and enigma. It's my first time with it haha today marks 20 days since fc and it's going hard!! I highly recommend a flow hood if you can afford 450$. Lab rat hoodz has AMAZING hoods for the price, but they take like 4 months to reach your doorstep after ordering. 2 man operation build on order... but hey, keep up with the agar. I use to do spores, but ITW has amazing LC. I grew that out and cloned what came from that. All in all tho, my grows are fucking fantastic now... stick with it, try things... if you ever struggle with trich just add 50g lime to bucket tek boom no more trich
materials necessary: barley malt extract -- brewery supply nutritional yeast -- a nice grocery store petridishes -- in example, pack of 25, online or lab supply perifilm (to seal the petridishes), online or lab supply agar agar - science lab type supply stores scale for grams, one liter + measuring cup, a bottle with good cap - 750 ml recomd. (to fit inside of a pressure cooker) small funnel, polystuffing (for bottle cap) pressure cooker other typical lab stuff you already have -- scissors, gloves, masks, alcohol, and hepi-filter or alt. Malt Extract Agar Recipe, for every one LITER of warm water: 20g Malt Extract 20g Agar Powder 2g Nutrional Yeast useful for : making clones, developing from spores, transfer culutures, making spawn, storing/ maintaining a culture bank
Your videos are the best on youtube for mushroom growing. Im sorry to point out that u didnt mention putting water in the pressure cooker. Ive never used a pressure cooker and almost started mine without water. I went back to the part in the video where you put the bottle in the pressure cooker and saw water in the bottom. I love your videos and pray for more content from you, im just worried that a newby may not add water to his cooker... love your work.
Thank you for the great information! I've been using your agar recipe to grow oyster mushrooms and have had very good results. Also, I love your dog in the background...very stoic!!
Your videos are great thank you! Curious why you wouldn't just pour the unsterilized Agar solution into the petri dishes and sterilize them all in a bag in the PC. Wouldn't that eliminate all that contamination risk of opening the sterilized agar solution and pouring into petri dishes?
Excellent video. I recommend using a canning jar, not a whiskey bottle. If you must use a bottle, choose one that has rounded corners at the bottom. If it has an abrupt transition from the walls to the bottom, it is much more likely that the bottom will break off the bottle when heating or cooling. An abrupt change in the thickness of the glass could also be a problem. The ideal container for heating or chilling is either borosilicate glass or spherical in shape or both. (Chemists use round-bottomed flasks.) The closer your bottle is to this ideal, the less likely you'll lose your bottle and contents in the pressure canner. BTW, pressure canners are moderately common at garage sales and flea markets. I've bought two this was and passed up many more. Not all of them are as large as Tony's seems to be, but I've never seen one that couldn't handle seven 1-quart jars.
Short sweet and best information !! Thanks Few questions 1) After inoculations, how to incubate these agar plates? Do this really require BOD incubator? Or any cost saving ideas? 2) How many times can we subculture these primary culture? Does passaging multiple times affect productivity? Looking forward for your experienced guidance
For brewers out there, 20g of dry malt extract per liter is about 1.8°P or 1.007 S.G. Basically, you could take your final runnings and water it down to 1.8°P or 1.007 S.G. just add the 20g of agar agar and nutritional yeast, and your good.
How long can you keep the petridishes with agar? Do you have to keep them refrigerated? When it is being inoculated with mushroom spores/mycelium how long can you keep it?
Man I love your videos. You give so much important information in a quick and easy to understand way. I like watching your videos as much or more than I enjoyed Roger Rabbits cultivation class videos that I had to pay for!
Thanks for the awesome vids! I am struggling to find malt extract, I have collected everything else but that. Ive been looking all over. Please tell me if and what substitute I could use as a replacement ?
Hola podias subtitular, es tan interesate lo que mostras de los hongos. Yo cultivo desde hace 20 años. Pero siempre miro video para aprender mas. Gracias.saludos desde argentina!
Tony you're awesome; I just subscribed! Very clear and well-paced. 👍🏻 Question, can you use a different medium than agar? For instance, gelatin, or carageenan?
NO. You're better off using the agar. Gelatin is an animal base product (collagen) and has a thin consistency unlike the plant-based agar. Also, it requires refrigeration to stay firm. so it's not a good replacement. I also heard that it can dry up very easily causing it to fail. I would think that mushrooms prefer the plant-based medium anyways.
in our lab we would autoclave the agar in the dish. that is much easier and contamination is minimized than pouring into dishes. just be sure to use glass dishes.
I liked the video because i like your stacking while pouring technique seems like it will help my arms a lot. Also folding the parafilm is a good idea makes it a little stronger so doesn't rip if you pull too hard
Have you considered adding an antibacterial component to your agar? I am curious if that would allow you to skip the sterilization process because it would eliminate bacteria and potential mold growth.
You can also add 500mg of amoxiciline, peniciline, doxiciline, cefalexine for every 500ml of agar solution after sterilization, be aware high temps destroy antibiotics, you can also add t your agar mix around 3gr of powdered charcoal before sterilization.
Background sound is a distraction to listening for some of us older folks 76, just saying. Thanks for the info you’re providing. I hope the answer is soon to come on your video.
Ever tried to sterilize plates under UV light instead of pressure cooking? Just thinking it might be a way to streamline the process and have some other benefits, provided it's actually effective... Basically, 1) make your agar mix 2) bring it to temperature in a pot on a stove until everything dissolves 3) pour your plates, leaving the lids off to avoid condensation 4) wait for the plates to cool off, place the lids on, seal with parafilm 5) place under germicidal UV for 5-10 minutes. Any thoughts/experience? Seems like it would cut down on time, eliminate the need for a pressure cooker, and drastically reduce the need to care about contamination (since you're sterilizing at the end after everything else is done). So no need for a flow hood or SAB.
I have a UV wand , as an added sanitary measure, in addition to alcohol. Personally, I have doubts that it does anything at all, but I would not use it in place of tried and true sterilization methods. Even with a SAB, and scrupulous sanitary and sterile techniques, plus UV I still get contam . Do everything reccomended, and then do it again, and more of it. I havent tried anti biotics or peroxide but some swear by it. NOTE: a little contamination when fruiting isnt the end of the world. The shrooms can win. But in LC, its the kiss of death. I am learning agar now. By all accounts, quickest and safest method. Don't know why so many still do spore to spawn, to substrate and hope for the best.
Awesome video, question: where i live the only barley extract i can find is in liquid form- dark color- looks like maple syrup. Would that do the trick or does it have to be powder? Cheers
Hello! In hope that you see this comment and question. I was wondering if it were fine to grow mycelium in a plain agar plate... I have lots of agar plates at home and wouldn’t want to waste them. Thanks so much
Excellent presentation. One question. You are working directly in front of the filter, so is it blowing toward the agar dishes or sucking air from the agar dishes? Or does it matter?
Hey man love your work. Both your videos and the very well organized blog. I am trying to make agar plates for ultimately home grown yummy mushrooms using coffee grounds. I would study your content. Also I would love to know what wordpress theme (or what tool) did you use for the blog. I love the side index that moves to scroll.
For years ive microwaved the agar to boil it before i put in the PC, but i see alot of people just mixing & then sterilizing.. pssh ive had so many overflows over the years i cant believe i couldve skippes that step!
How to wash glass plates that are reusable? If you grow the fungus on plates without agar, does it multiply in the same way but more slowly or does it not multiply on its own?
Thank you Tony for all the mycoinfo you’re sharing. I have some dowl inoculated shiitake logs that are slowly flushing for their first time. I have access to an industrial autoclave and lfh as well as YM (yeast/mold) nutrient agar. Is this media suffient for growing shiitake culture? What type of streak or inoculation method do you prefer when transferring from spore print to plate?
To update, i picked my shiitake cap a bit too soon and didn’t get a good spore print. I had good success cloning from a cap and now have 4 quarts of healthy grain spawn and agar plates. My plan is to use the grain spawn to inoculate 500 dowels for logs this spring and start some fresh jars of grain spawn as well. I also have isolated a wild oyster (yellow?). It’s amazing how much faster the mycelium grows compared to shiitake. I’m going to inoculate substrate blocks this weekend and hopefully have indoor oysters this winter. Thank you Tony and Teegan for all your helpful videos and blogs!
Thanks for watching! Get the full article here: learn.freshcap.com/growing/agar-agar-so-nice-they-named-it-twice-5-steps-to-pouring-perfect-plates/ Have FUN growing mushrooms :)
Hey whats the best temperature to store your agar and syringes in your lab fridge
Best editing and fastest presentation of information I've ever seen on UA-cam. You are a true artist, Tony.
"Trifles make perfection, and perfection is no trifle. Genius is eternal patience." - Michelangelo Buonarroti
Wow thanks so much for the kind words!
I personally think this is second best to Mark Keith's tequnique. He just missed a couple of important notes and tips. Agar solidifies at around 120°f and pouring as cool as you can reduces condensation. I'm also not partial to how he handles the dishes after they have cooled. The insides can be assumed sterile but to really go the extra mile you could place them in a sterile container before shutting the flow hood off. You likely aren't going to pressure cook a tote to put your petri dishes in, and sanitizing a tote is second best. So where do you find a sterile container suitable? The bag they came in is sterile, so if you roll it up and place it under your rack, once your plates are set you can put them right back in the bag, then you dont need to waste the parafilm and dont risk drying out your media. Is this technique straightforward and to the point? Yes. It's very professionally done in both style and production. Please take this as constructive criticism, I'm not bashing at all.
Mason Lundy great, great tips. Thank you so much!
@@FreshCapMushrooms I was wondering if you would be able to do a video on how to spec out and make a 24x24 flow hood and maybe a parts list. I know you did a video on making a 24x48 flow hood but I just think that's to big and expensive for the stage that I am at right now. Side note, I live your videos, the first one I seen was the bucket tek on and that just got me so interested in growing mushrooms. Thank you again for all the info and videos you do. Keep up the amazing work. I don't know if you coild maybe email me the instructions if you don't want to make a video. I'm finding so much information on UA-cam and it is kind of overwhelming and I don't know who to believe and follow hence why I'm asking you I feel that the knowledge that you have in the videos that you have done so far have been an amazing help to me so far
dude, where have you been all my life? i've been learning mycology for a while now and had so many simple yet significant questions that all other online sources have failed to answer until now. thank you and keep up the good work! i'm so happy to find someone willing and capable to include the attention to detail that is so necessary to succeeding in this field. i will be recommending your videos to all my noob myco peers, and hopefully before too long i will have developed the skills and confidence to share my own wisdom via quality video tutorials like these. respect :)
How does someone get into mycology? Sounds interesting
Multisanchez.. have an interest for starters.
Bill Palmer of course, of course ^^'
ممكن لو سمحت تعطيني بعض النصائح انا ماعم أنجح ابدا
@@sanamahmod8610 ask the right questions
I have an exam tomorrow on Mushroom cultivation technology and I can't explain how helpful your videos are proving to me. Your explanations and physical demonstration are so easy to understand. My book is really annoying and I was having a hard time preparing for tomorrow so thank you for such amazing content. And writing this currently, I have already watched 10 of your videos.
You are so wonderfully informative. By teaching the ignorant like myself, you are changing lives. It takes a community to save the world, a great start.
I like how the dog looks through the window. Very calm.
Accidentally had my playback at 1.5 he was very up beat
My playback usually sits at x2.5 I got things to do, places to go, people to see. :P
heyou yea definitely I keep my playback at the max. Watch 4 videos in the time of 1 😂😂
😂
@@joshualowe79 what???? Duh..u uped my info intake by 4. Lol wow. I'd have a doctorate by now
This comment is too funny
Great video, very informative, quick and to the point. No wasted banter and you covered everything! Thanks
Great Video! As a passionate Home Brewer I can tell you that you‘ll find all the materials in good brew stores. Including petri dishes Agar agar and off course the rye which is used in the mason jars. These materials are also used for yeast cultivation.
Thanks for that info! Home brewing is something I'm learning about as well as mycology.
I used a pressure cooker for the first time today. I also made agar dishes and innoculated them for thr first time. First grow ever actually, hoping it goes well but if not thats why i chose to learn the agar! Thanks !
How are your grows going? I'm just starting agar work rn.
@markgarry7866 by now amazing man. Currently I have 3 LC's I work with TAT, PE, and enigma. It's my first time with it haha today marks 20 days since fc and it's going hard!! I highly recommend a flow hood if you can afford 450$. Lab rat hoodz has AMAZING hoods for the price, but they take like 4 months to reach your doorstep after ordering. 2 man operation build on order... but hey, keep up with the agar. I use to do spores, but ITW has amazing LC. I grew that out and cloned what came from that. All in all tho, my grows are fucking fantastic now... stick with it, try things... if you ever struggle with trich just add 50g lime to bucket tek boom no more trich
Best tutorial I have seen.
materials necessary:
barley malt extract -- brewery supply
nutritional yeast -- a nice grocery store
petridishes -- in example, pack of 25, online or lab supply
perifilm (to seal the petridishes), online or lab supply
agar agar - science lab type supply stores
scale for grams, one liter + measuring cup,
a bottle with good cap - 750 ml recomd. (to fit inside of a pressure cooker)
small funnel, polystuffing (for bottle cap)
pressure cooker
other typical lab stuff you already have -- scissors, gloves, masks, alcohol, and hepi-filter or alt.
Malt Extract Agar Recipe, for every one LITER of warm water:
20g Malt Extract 20g Agar Powder 2g Nutrional Yeast
useful for :
making clones, developing from spores, transfer culutures, making spawn, storing/ maintaining a culture bank
Your videos are the best on youtube for mushroom growing. Im sorry to point out that u didnt mention putting water in the pressure cooker. Ive never used a pressure cooker and almost started mine without water. I went back to the part in the video where you put the bottle in the pressure cooker and saw water in the bottom. I love your videos and pray for more content from you, im just worried that a newby may not add water to his cooker... love your work.
It's like instant noodles, just add water.
It's all so easy when you get beyond the difficulty.
Great information Tony. How long will the agar keep before it’s not useable?
Tony is the best guy, when it comes to disseminating Mycology Knowledge! ❤️
I have learned so much from you! Please don't stop teaching!
Thank you for the great information! I've been using your agar recipe to grow oyster mushrooms and have had very good results. Also, I love your dog in the background...very stoic!!
Your videos are great thank you! Curious why you wouldn't just pour the unsterilized Agar solution into the petri dishes and sterilize them all in a bag in the PC. Wouldn't that eliminate all that contamination risk of opening the sterilized agar solution and pouring into petri dishes?
Assuming you can get Glass Petri dishes that is...obviously plastic will melt
A whiskey bottle? Tell me you're from Alberta without telling me you're from Alberta...
Love it!
Great job on the clip..
Very informative and clearly presented I will be trying soon. I need to make a flow hood .
Excellent video.
I recommend using a canning jar, not a whiskey bottle. If you must use a bottle, choose one that has rounded corners at the bottom. If it has an abrupt transition from the walls to the bottom, it is much more likely that the bottom will break off the bottle when heating or cooling. An abrupt change in the thickness of the glass could also be a problem. The ideal container for heating or chilling is either borosilicate glass or spherical in shape or both. (Chemists use round-bottomed flasks.) The closer your bottle is to this ideal, the less likely you'll lose your bottle and contents in the pressure canner.
BTW, pressure canners are moderately common at garage sales and flea markets. I've bought two this was and passed up many more. Not all of them are as large as Tony's seems to be, but I've never seen one that couldn't handle seven 1-quart jars.
Great video Tony. Very helpful and professional. We really enjoy your Lions Mane and Cordycepts supplement powers.
Short sweet and best information !! Thanks
Few questions
1) After inoculations, how to incubate these agar plates? Do this really require BOD incubator? Or any cost saving ideas?
2) How many times can we subculture these primary culture? Does passaging multiple times affect productivity?
Looking forward for your experienced guidance
For brewers out there, 20g of dry malt extract per liter is about 1.8°P or 1.007 S.G.
Basically, you could take your final runnings and water it down to 1.8°P or 1.007 S.G. just add the 20g of agar agar and nutritional yeast, and your good.
Dude, your channel is awesome. Thanks for sharing so much valuable information.
How long can you keep the petridishes with agar? Do you have to keep them refrigerated?
When it is being inoculated with mushroom spores/mycelium how long can you keep it?
You produce the best condensed videos of lots of great information and cool little tips that made my life easier. Thanks
Amazing content but I have been getting contaminations do I need to sterile the paralfim I do it in still air box
Can you store agar dishes in the fridge? or what temp /environment is best in your opinion?
yeah the fridge is good for storage. I just used some that had been in the fridge for 6 months and they seem fine.
i would say 2°Celcius , not 1 degree below
How many times can I use spores from either spore prints, liquid culture, or fruiting body before the genetics are weakened noticeably?
Man I love your videos. You give so much important information in a quick and easy to understand way. I like watching your videos as much or more than I enjoyed Roger Rabbits cultivation class videos that I had to pay for!
Awesome! Glad you like the videos :) RR's videos were gold- pretty sure he made those before UA-cam was really a thing :)
roger rabbit can suck it (hence the lower case r's) I have always found him so arrogant in the forums.
how do you clean the bottle afterwords ? after the residual in the bottle solidifies...?
TaThank you from thousands of miles away for the tutorials
does anybody know if i can use liquid malt extract instead of powder?
Thank you for this video. I’m very new to all of this and videos like yours make the whole process less daunting.
Thank you Professor🙏
How long do the agar dishes last? Do you put the tape on them after they agar has solitified or before?
Parafilm is the coolest material on earth. I used to LOVE playing with that shit when I used to work at a microbiology lab.
Great technique! Getting good at handling plates is like getting good at shuffling cards.
Thanks for the awesome vids!
I am struggling to find malt extract, I have collected everything else but that. Ive been looking all over. Please tell me if and what substitute I could use as a replacement ?
I learned so much . Thanks a lot for your valuable time. Thanks for Sharing your knowledge.
Great video.
Just a quick question.
How long will the agar in the petri dish keep for, if i was to make them to be used at a later date?
In theory, if no contamination / cracks due to drying are visible, they are still good. We stored them in cold chambers for months
How long can you store them
Nice! I´m gonna buy some whisky just to try this in a couple of days after I´m done with the whisky and with the hangover. XD
Thanks, you couldn't have explained that any better. I wish more people videos would just get down to basics.
Your the greatest👍🏻 always spot on explanations for us to try out our luck at a great hobby or possibly a business
I would like to learn this to save money and have as a hobby. The whole process is super cool
Thank you, Tony, for another extremely helpful tutorial!
Hola podias subtitular, es tan interesate lo que mostras de los hongos. Yo cultivo desde hace 20 años. Pero siempre miro video para aprender mas. Gracias.saludos desde argentina!
After making your Agar plates what is its shelf life (proper long term storage)? 1 - Storage without being cultured 2 - storage with culture?
until it dries out
Tony you're awesome; I just subscribed! Very clear and well-paced. 👍🏻
Question, can you use a different medium than agar?
For instance, gelatin, or carageenan?
NO. You're better off using the agar. Gelatin is an animal base product (collagen) and has a thin consistency unlike the plant-based agar. Also, it requires refrigeration to stay firm. so it's not a good replacement. I also heard that it can dry up very easily causing it to fail. I would think that mushrooms prefer the plant-based medium anyways.
can I reccomend losing the background music. For someone with noise sensitivity, it makes it difficult to listen to you
At least lower the volume
@@immunesicness4950 that is impossible to do she puts the video on and it replays for entire year at full volume
About how long will plates last after being made?
By far the best video on mushrooms👍
Is there a cheaper alternative to the parafilm?
in our lab we would autoclave the agar in the dish. that is much easier and contamination is minimized than pouring into dishes. just be sure to use glass dishes.
coolest job done tony loved your script best defined and executed.
Such a great video. Can the actual plates be cleaned out and reused later for fresh agar?
I liked the video because i like your stacking while pouring technique seems like it will help my arms a lot. Also folding the parafilm is a good idea makes it a little stronger so doesn't rip if you pull too hard
Have you considered adding an antibacterial component to your agar? I am curious if that would allow you to skip the sterilization process because it would eliminate bacteria and potential mold growth.
Lisette Land you could use hydrogen peroxide if your growing mycelium
You can also add 500mg of amoxiciline, peniciline, doxiciline, cefalexine for every 500ml of agar solution after sterilization, be aware high temps destroy antibiotics, you can also add t your agar mix around 3gr of powdered charcoal before sterilization.
Background sound is a distraction to listening for some of us older folks 76, just saying. Thanks for the info you’re providing. I hope the answer is soon to come on your video.
Good video, I like to add a little bit of food color, green specifically. It shows contamination better.
i've only ever used green agar since the beginning. good to see someone else who agrees :)
Agreed
marcman5109 hello, so the food coloring is added at the very beginning of the mixture ?
Ever tried to sterilize plates under UV light instead of pressure cooking? Just thinking it might be a way to streamline the process and have some other benefits, provided it's actually effective...
Basically, 1) make your agar mix 2) bring it to temperature in a pot on a stove until everything dissolves 3) pour your plates, leaving the lids off to avoid condensation 4) wait for the plates to cool off, place the lids on, seal with parafilm 5) place under germicidal UV for 5-10 minutes. Any thoughts/experience?
Seems like it would cut down on time, eliminate the need for a pressure cooker, and drastically reduce the need to care about contamination (since you're sterilizing at the end after everything else is done). So no need for a flow hood or SAB.
I have a UV wand , as an added sanitary measure, in addition to alcohol. Personally, I have doubts that it does anything at all, but I would not use it in place of tried and true sterilization methods. Even with a SAB, and scrupulous sanitary and sterile techniques, plus UV I still get contam . Do everything reccomended, and then do it again, and more of it. I havent tried anti biotics or peroxide but some swear by it. NOTE: a little contamination when fruiting isnt the end of the world. The shrooms can win. But in LC, its the kiss of death. I am learning agar now. By all accounts, quickest and safest method. Don't know why so many still do spore to spawn, to substrate and hope for the best.
Great video! Precise and great presentation. Just a damn solid video!
could i also use micropore tape instead of polyfil when sterilizing?
For how long can Agar Agar be stored in Petri dishes before you have to use it.?
Does nutritional yeast flakes work?
This is a great we'll done video, but then what? How does this eventually equal mushrooms?
I have that same Tupperware funnel lol don't know why that makes me so happy lol
I'm pretty sure in college my lab would pour the agar into the Petri dishes. Then pop them into the steriliser
Well I hope malt extract will work for the barley. Cause that what I have, so I'm going to use it.
Hey Toby, I just love your vids, can you put the agar in the petri dishes and then sterilise the petri dish??
Great videos! Just wondering if you ever plan on doing a liquid culture video?:-)
Great video..great presentation
One thing I use is the grain water from simmering is used to make my agar plates instead of plain water
Is there another name for "parafilm"?
I don't know what it is in my language. Thanks in advance
I wonder if this recipe works for all species of mushrooms
Awesome video, question: where i live the only barley extract i can find is in liquid form- dark color- looks like maple syrup. Would that do the trick or does it have to be powder? Cheers
Hello! In hope that you see this comment and question. I was wondering if it were fine to grow mycelium in a plain agar plate... I have lots of agar plates at home and wouldn’t want to waste them.
Thanks so much
Excellent presentation. One question. You are working directly in front of the filter, so is it blowing toward the agar dishes or sucking air from the agar dishes? Or does it matter?
it is pushing clean air
Hey T, should I be concerned about the black build up on my blade after sterilization??
Good question. I was wondering that also.
Haventgot a pressure steriliser. What do i do?
Could someone use something other than barley malt? I can't medically have it.
Can I use an electric pressure cooker instead? I already have one and wondering if it's necessary to buy a stove one for this. :)
instead of polyfill for the air exchange at 2:02 could I use micropore tape instead?
I cut my parafilm in half it works well seals 2 dishes instead of 1
So is "nutritional yeast" the same as bakers yeast?
Thanks, I was looking for some pointers on how to apply the parafim tactfully.
What’s the yeast for? Can’t you just use the malt extract alone? :)
Where do i get the stainlesssteel rack for flow hood
Woah woah man, I know a sprain Morgan bottle when I see it
Hey man love your work. Both your videos and the very well organized blog. I am trying to make agar plates for ultimately home grown yummy mushrooms using coffee grounds. I would study your content. Also I would love to know what wordpress theme (or what tool) did you use for the blog. I love the side index that moves to scroll.
For years ive microwaved the agar to boil it before i put in the PC, but i see alot of people just mixing & then sterilizing.. pssh ive had so many overflows over the years i cant believe i couldve skippes that step!
How to wash glass plates that are reusable?
If you grow the fungus on plates without agar, does it multiply in the same way but more slowly or does it not multiply on its own?
Out of curiosity why are you adding the yeast? Wouldn’t that be a competing fungus?
Very good and clear explanation, more video please!! ;) Thanks for making this one! ;)
Awesome, I'm glad you found it useful! I will be working on many more videos in the near future.
How long do these last? Preference in fridge a good idea for shelf life?
Your videos are the best out here, thanks!
Thanks so much for sharing your knowledge!!! I can’t wait 🤩
Thank you Tony for all the mycoinfo you’re sharing. I have some dowl inoculated shiitake logs that are slowly flushing for their first time. I have access to an industrial autoclave and lfh as well as YM (yeast/mold) nutrient agar.
Is this media suffient for growing shiitake culture? What type of streak or inoculation method do you prefer when transferring from spore print to plate?
To update, i picked my shiitake cap a bit too soon and didn’t get a good spore print. I had good success cloning from a cap and now have 4 quarts of healthy grain spawn and agar plates. My plan is to use the grain spawn to inoculate 500 dowels for logs this spring and start some fresh jars of grain spawn as well. I also have isolated a wild oyster (yellow?). It’s amazing how much faster the mycelium grows compared to shiitake. I’m going to inoculate substrate blocks this weekend and hopefully have indoor oysters this winter. Thank you Tony and Teegan for all your helpful videos and blogs!