No words to express how much your videos are exactly the perfect content for an absolute beginner and also for someone who has been working with mushrooms for years. Thank you for your enthusiastic content.
I agree 100%. I fkg love this guy and his videos. Got my young kids (5 &6) to start watching with me and they love the silliness and the characters he does lol
@@DeathFromAbove_5.56 You do realize sheomery is technically out dated due to the drop in trust as well as pros still using that forum? Was a good run but it's been used to arrest ppl in the past so nah bro. Stop glowing.
Great video Paul, I've seen this and the mycology lab 101, and it has been some of the best long-form content on this website as of late. The quality of information and production is greater than quite a few of my university professors (albeit embarrassing on their behalf). Thank you!
Excellent timing! Thank you for your generosity- I ordered 1 starter syringe of oyster & received 3 extras!!! Wee! You are awesome! Thank you thank you! 🙏🏻 I have been slightly intimidated by the agar process but have my Petri dishes standing by & hopefully after your video will gain the confidence to begin. Mush Appreciation for your work & beautiful Spirit, Paul! ❤️❤️❤️❤️❤️❤️❤️❤️❤️
We have to be careful relying on 20 year old growing advice when we've advanced the hobby beyond that point. I mean look it up, peroxide is toxic to mycellium....homeboy is a rookie. Wallax2 doesn't want him here. GO BACK TO PORTLAND @FUNGIA
😂this dude is what I would say should be the face of the myco community… he’s not afraid to let his weird shine through and he understands the pain and the hurdles of starting out as a young mycologist dude I started crying when you said shoplifting flats of mason jars😂😂😂 you are a legend and when I die I want you to know that it may not be a lot but I have taken my wife out of my will and replaced her name with you…fr
I've been watching these over and over waiting for the next one. It's like rewatching that movie you just can't get enough of! The dude is authentic, knowledgeable, and a true genuine teacher! The humor is just right. My kids' favorite is the pirate character.
Great video as always! Thank you for giving us the directions and the confidence to explore while being fun and interesting. As someone who is new to this I can't understand why you don't have followers in the millions? Thanks again!!
Excellent, very timely, and informative video (for starters like me) giving glimpses of the shroomie enthusiasts and astonishingly, the fungi world pov. I am thinking you must be sharing a pack or two with mycophilia and maybe munchauzen. You guys are admirable in your honest to goodness presentations of your efforts in advancing the field of mushroom growing science. Congratulations!
Love the blaagar, i have a few odd recipes i refer to as slam tech. Lol pretty much 2-3% of watever i have laying around. Have even used the water from boiling black beans, also love using the run off when i ferment wood chips for my outdoor beds. For real anything that is carb/sugar/starch/yeast based will work
Fascinating. When I first got in to growing mushrooms I didn't realize that I'd have to become a micro guy! I just started, I injected spores into my substrate x4 and nothing happened. Great first failure LOL.
Thanks for the video it was very informative, I especially appreciated the detail on condensation, I noticed the scalpel blade was burned at the tip but no carbon deposits, I use a candle to sterilise but always have a build up of carbon. It looks really messy, especially with spores. Any tips for getting the blade sterile yet ‘clean’, thanks
Great videos and great explanations; I’m getting eager to start playing. Can you tell some about the “yeast” part? What type of yeast are you recommending? Wine yeast is good? Or beer? Thanks allot for the great content.
I just ordered your Black Agar and Pink Oyster LC syringe this morning. I'm hoping the Black Agar works for a variety of mushrooms Lions Mane, Turkey Tail, COW, Oysters etc.
So, I never have an issue with the media bottle. I always had issues with pouring from a jar. Now, i have to sut my media bottle at a tilt in my PC. I use a small jar to keep it lifted. While also keeping the lid slightly loose with foil on. Never had it boil over.
anyone have any insight on putting mycelium from a pf cake on an agar plate. It should work to clone right? I'm going to try no matter what just wondering what some of you think.
Yes. You can but keep in mind each exposure to open air will possibly introduce contamination. It also may be a bit clumpy depending on your recipe and age of agar.
do you have any tips for eliminating condensation when you only have a SAB to work with? pouring at 115 helped a lot but I still get some moisture and then I end up seeing contam where that moisture is sitting.
As a retired lab-rat turned mycologist, I've always used parafilm until recently. I switched to grafting tape and find it is much cheaper (0.4 cents per plate vs. 10 cents per plate for parafilm) and much faster since I don't have to cut the grafting tape into strips like I did for parafilm. Takes a little getting used to if you're already familiar with parafilm but well worth learning. Since grafting tape is so cheap I do seal every plate after the agar is set before storing. Something I didn't do with parafilm.
I just mix up my agar media in a sauce pan and when ready, take 1/2 pint canning jars, small and wide mouth work and fill them to the same level I would do in a petri dish. Then I put an injection port with a micron filter patch lid on the jars and load up my pressure cooker and sterilize as usual. If I want culture slants, I prop one side of the pressure cooker up while letting it cool or just let it cool to room temp and unload the jars in my cleanroom onto a wire rack for draining (okay, I really take a bunch of paper towels and soak them with rubbing alcohol and let them dry.) and now I don't need to pour the sterile solution & run the risk of contamination while filling the petri dishes. I have clean and viable cultures in plastic bags in my fridge that are years old using this method. I also don't have to reopen the petri dish to inoculate it, or wrap the edges in sealing tape. Just wipe off the injection port and make my injection. Wrapped in plastic bags to stop the slow evaporation through the microfilter patch and these last for years at room temp.
@_fungaia How do you prevent it from the agar from cracking once it is poured and set to dry? I noticed some plated when I go to bag them, some of the agar inside the petri dishes are cracked so to speak.
Thank you for the video. I think, regular jello should work too. Regular gelatin melts at 38 deg C in an incubator. That temperature is used for bacterial growth copying human body temperature. Fungal cultures are incubated, usually, at room temperature 18 deg C to 20 dec C. Gelatin stays jello.
I watch these videos but remember when I watched someone just take mushrooms and chuck them into a blender and layer them with some media in a pattern and then watered them to end up with mushrooms in a few weeks. How is the lab method different or superior? Just wondering if it does anything special.
Thank you for your awesome videos, and your great products! Keep doing exactly what you do sir! Thank you😁 I just noticed all the sound equipment behind you 😯🔥
Do you have any evidence or proof that adding your substrate or all of that extra stuff makes any difference? Or helps the mycelium to 'recognize it later' as you say? I'm just curious.
imho and maybe and all due respect and thank you for the video Isolation of microbes: a mixed culture can be inoculated on the surface of the media about 1/3, 1/4 of . After that the inoculation loop is sterilized and from the inoculated surface using one streak the microbes can be carried to other, sterile side of the media surface in the petri dish. This produces a dilutional effect and the microbial growth shows up on the diluted surfaces a single colonies making it possible to transfer colonies of a single organism to another petri dish. Mixture of microbes can be isolated into colonies grown of single, one kind of microbes. Pure colonies can be obtained this way. imho and maybe and all due respect and thank you for the video
Oh man, yes, you're correct. That's an embarrassing and misleading mistake. 0.01%-0.05% is the correct dilution, 0.3% is way too much! I can't believe I didn't catch that. I'll post a correction in the description, thank you.
This dude is the new, cooler Bill Nye the Science Guy. I'm a big fan.
This is excellent content for getting youth involved in science of mycology, like Bill Nye did for science generally when I was a kid.
Hes Bill Nye's nephew
Really or are you saying this as a compliment @@mindmelt889
Me too. Me too
bill nye is not a scientist and actually is a cucko tv talk host for globalist, do not be funny, this guy is the real deal.
No words to express how much your videos are exactly the perfect content for an absolute beginner and also for someone who has been working with mushrooms for years. Thank you for your enthusiastic content.
I agree! Paul’s enthusiasm is fantastic and he’s in fact an excellent teacher.
I agree 100%. I fkg love this guy and his videos. Got my young kids (5 &6) to start watching with me and they love the silliness and the characters he does lol
This is a criminally underrated channel! I love it when our dude does the pirate and other character impressions like the professor. lol
Dude has character and extremely useful information I have not found elsewhere. AAAARGGGHHHHH
You mean like a SpongeBob knock off pirate? "Aree you ready kidsssss"... Yeah we see it.
@@DeathFromAbove_5.56 You do realize sheomery is technically out dated due to the drop in trust as well as pros still using that forum? Was a good run but it's been used to arrest ppl in the past so nah bro. Stop glowing.
This is easily one of the best agar videos on YT. Great explanation, entertaining, and very useful tips.
Great video Paul, I've seen this and the mycology lab 101, and it has been some of the best long-form content on this website as of late. The quality of information and production is greater than quite a few of my university professors (albeit embarrassing on their behalf). Thank you!
you gave just the right amount of parenthesis and unusual words, such as albeit, to prove to us you went to college, but where is your semicolon?
This is the best free mushroom cultivation educational channel on the internet! Thank you so much Paul from Caracas Venezuela!
Excellent timing! Thank you for your generosity- I ordered 1 starter syringe of oyster & received 3 extras!!! Wee! You are awesome! Thank you thank you! 🙏🏻 I have been slightly intimidated by the agar process but have my Petri dishes standing by & hopefully after your video will gain the confidence to begin. Mush Appreciation for your work & beautiful Spirit, Paul! ❤️❤️❤️❤️❤️❤️❤️❤️❤️
This was exactly what i needed this morning, you are a legend!
Sweet! Just started in mycology and I love learning all these different agar recipes! I can’t wait to watch it!
We have to be careful relying on 20 year old growing advice when we've advanced the hobby beyond that point. I mean look it up, peroxide is toxic to mycellium....homeboy is a rookie. Wallax2 doesn't want him here. GO BACK TO PORTLAND @FUNGIA
😂this dude is what I would say should be the face of the myco community… he’s not afraid to let his weird shine through and he understands the pain and the hurdles of starting out as a young mycologist dude I started crying when you said shoplifting flats of mason jars😂😂😂 you are a legend and when I die I want you to know that it may not be a lot but I have taken my wife out of my will and replaced her name with you…fr
looking forward for your diy laminar flow hood video. its really expensive to ship your stuff to EU. love the videos tho, keep it up!
Amazing video on DIY Agar plates!! Thank you for sharing ❤
I've been watching these over and over waiting for the next one. It's like rewatching that movie you just can't get enough of! The dude is authentic, knowledgeable, and a true genuine teacher! The humor is just right. My kids' favorite is the pirate character.
Love your channel. Reinspiring me as a grower.
I had to rewatch this 4 times, good info that I wanted to Wright down and try out. Thank you!!
I like you Channel very much, as im currently starting to grow my own shroomies. I like the way you explain things. You are a very likable guy.
Dude you’re a natural educator and the camera loves you. Thanks heaps for another awesome video 👍🏼🫶🐶
Great video as always! Thank you for giving us the directions and the confidence to explore while being fun and interesting. As someone who is new to this I can't understand why you don't have followers in the millions? Thanks again!!
Awesome agar tutorial. A mountain of time and love must have gone into the creation of this much appreciated video.
Not only great as an informative guide, but an entertaining content creator. Great video!
This content is absolutely incredible. So much diverse info synthesised in one place. Thank you, Paul.
Love what your doing and how you're doing it
Love the content. It's very informative and quite enjoyable to watch. I've already learnt a bunch.
Yo this content is beyond impressive! Informative. Concise. Creative and captivating delivery. Subbed!
Great stuff. Thanks.
A fan after 2 videos. Awesome work dude.
Hey dude your channel is pretty great, thank you
You really do a good job of breaking it down. Appreciate the videos
All good... wish there was the internet when I was doing this back in the 70's... I like the charcoal additive, creative!
Thanks Professor. I really enjoy your videos and all your great info. You’re a true pleasure to watch. Looking forward to more great videos.
It's such a shame that I can only like your videos once! Thanks for all the amazing content :)
Just received my order and I've never seen mycelium so thick and healthy in a syringe 😮🎉 100% killing it
We live up north of you in Okanogan county! I just started my mushroom growing journey and you have such great videos!
Excellent, very timely, and informative video (for starters like me) giving glimpses of the shroomie enthusiasts and astonishingly, the fungi world pov. I am thinking you must be sharing a pack or two with mycophilia and maybe munchauzen. You guys are admirable in your honest to goodness presentations of your efforts in advancing the field of mushroom growing science. Congratulations!
This video was a little grainy 😁 Thank you so much, loving your free education ❤🍄
Here's a SUPER THANKS! You are the best!
Love the blaagar, i have a few odd recipes i refer to as slam tech. Lol pretty much 2-3% of watever i have laying around. Have even used the water from boiling black beans, also love using the run off when i ferment wood chips for my outdoor beds. For real anything that is carb/sugar/starch/yeast based will work
I love your organization. I wish I had the space to do agar and cultures. I'm limited to ordering spores or cultures for now.
I made a mesh table for my SAB
And I made it a little slanted
I have been pooring plates slanted for months and I love it
I learned alot! Thankyou.
Fascinating. When I first got in to growing mushrooms I didn't realize that I'd have to become a micro guy! I just started, I injected spores into my substrate x4 and nothing happened. Great first failure LOL.
Awesome stuff! I look forward to more content.
Saludos desde Chile. Me encantan tus videos! ❤🍄
Thanks for the video it was very informative, I especially appreciated the detail on condensation, I noticed the scalpel blade was burned at the tip but no carbon deposits, I use a candle to sterilise but always have a build up of carbon. It looks really messy, especially with spores. Any tips for getting the blade sterile yet ‘clean’, thanks
Media flask with pouring ring is king 💪
Great videos and great explanations; I’m getting eager to start playing.
Can you tell some about the “yeast” part?
What type of yeast are you recommending? Wine yeast is good? Or beer?
Thanks allot for the great content.
Thank you for the videos!! ❤❤ They're super helpful!
I'm glad!
Totally re-ignited my agar excitement. I have all the tools and material/ingredients I need, just never quite got around to it
I wish I could like this video 1000x ⭐️⭐️⭐️⭐️
Can't wait for that library video
I just ordered your Black Agar and Pink Oyster LC syringe this morning. I'm hoping the Black Agar works for a variety of mushrooms Lions Mane, Turkey Tail, COW, Oysters etc.
So, I never have an issue with the media bottle. I always had issues with pouring from a jar.
Now, i have to sut my media bottle at a tilt in my PC. I use a small jar to keep it lifted. While also keeping the lid slightly loose with foil on. Never had it boil over.
Wow those are big speakers! Fungi Rock!
Another wicked video
anyone have any insight on putting mycelium from a pf cake on an agar plate. It should work to clone right? I'm going to try no matter what just wondering what some of you think.
Could you explain why the yeast? Doesn't it oppose the fungus?. TKs
I think yeast is a marker of wrong sterilization
Hi i have the 8qt Instant Pot Pro & wondering how long on HIGH to sterilize Agar & brf jars?
Ty, this is great. I might have missed it, but... what if I have agar leftover? Can I store it for later and reheat it and pour more petris?
Yes. You can but keep in mind each exposure to open air will possibly introduce contamination. It also may be a bit clumpy depending on your recipe and age of agar.
@@TheTubejunky Thanks 🤙
do you have any tips for eliminating condensation when you only have a SAB to work with? pouring at 115 helped a lot but I still get some moisture and then I end up seeing contam where that moisture is sitting.
Dude! Hiw loud do you listen to your music? 😂 the size of those speakers! Love you content 👍🏻
i bet those old peaveys still crank!! hehe niiice!
I use grafting tape instead of the specialized tape. I guess it's only an issue if you do no-pour agar?
As a retired lab-rat turned mycologist, I've always used parafilm until recently. I switched to grafting tape and find it is much cheaper (0.4 cents per plate vs. 10 cents per plate for parafilm) and much faster since I don't have to cut the grafting tape into strips like I did for parafilm. Takes a little getting used to if you're already familiar with parafilm but well worth learning.
Since grafting tape is so cheap I do seal every plate after the agar is set before storing. Something I didn't do with parafilm.
Thank you! Yeah I have so very much to learn.@@dthomas021
Damn. Do yall be having dj sessions where you give your presentation? Nice speakers lol
I just mix up my agar media in a sauce pan and when ready, take 1/2 pint canning jars, small and wide mouth work and fill them to the same level I would do in a petri dish. Then I put an injection port with a micron filter patch lid on the jars and load up my pressure cooker and sterilize as usual. If I want culture slants, I prop one side of the pressure cooker up while letting it cool or just let it cool to room temp and unload the jars in my cleanroom onto a wire rack for draining (okay, I really take a bunch of paper towels and soak them with rubbing alcohol and let them dry.) and now I don't need to pour the sterile solution & run the risk of contamination while filling the petri dishes. I have clean and viable cultures in plastic bags in my fridge that are years old using this method. I also don't have to reopen the petri dish to inoculate it, or wrap the edges in sealing tape. Just wipe off the injection port and make my injection. Wrapped in plastic bags to stop the slow evaporation through the microfilter patch and these last for years at room temp.
Cool DIY method, thanks for sharing!
that was great video thank you
@_fungaia
How do you prevent it from the agar from cracking once it is poured and set to dry? I noticed some plated when I go to bag them, some of the agar inside the petri dishes are cracked so to speak.
You are amazing! Thank you
Not sure what kind of glove it is but I put a heat proof glove under a nitrile glove to keep from burning my hand when I pour the agar
Nicely done sir!
Great video!
Hahahahha LOVE the jar label ‘Donkey Sh**’!!! 😂
What type of alcohol is used In the little lamp?
Denatured I think?
do the speakers help with mushroom cultivation?
Thank you for the video.
I think, regular jello should work too. Regular gelatin melts at 38 deg C in an incubator.
That temperature is used for bacterial growth copying human body temperature. Fungal cultures are incubated, usually, at room temperature 18 deg C to 20 dec C. Gelatin stays jello.
Appreciate the information! ❤
Amazing 👏🏽
Bought from true blue 21$ for LC plus shipping. Awesome deal. My other vendor 40$ with shipping. I will come back with reviews
You're Awesome!
Like the videos. Subscribed. 👍
🍄Thanks, Capt'n! 🎇🪄✳🗿🧫🕯🎑🐉🧑🎤👽
wait, no parafilm?
I watch these videos but remember when I watched someone just take mushrooms and chuck them into a blender and layer them with some media in a pattern and then watered them to end up with mushrooms in a few weeks. How is the lab method different or superior? Just wondering if it does anything special.
I really need to try this BLAGAR recipe 😊
By the way, how much activated carbon did you add to 500ml of water?
A good starting point to try is 2-3 grams for 500ml
He is the king👑🔥🌹
What's the name of the intro song? ❤
thanks Paul. now i can eat mushrooms till the end
Thank you for your awesome videos, and your great products! Keep doing exactly what you do sir! Thank you😁
I just noticed all the sound equipment behind you 😯🔥
upload more please
Do you have any evidence or proof that adding your substrate or all of that extra stuff makes any difference? Or helps the mycelium to 'recognize it later' as you say? I'm just curious.
Yes, I have run numerous controlled trials. In some cases pre-exposure has cut incubation in half!
Are you using chlorinated tap water for your agar?
I love that you ate the agar😅
Please keep making videos
What would you recommend for Lion's Mane agar? Thank you
Any of the recipes mentioned work great for Lion's Mane!
@@_fungaia thank you, oh wise blagar pirate 🙇🏻
The speakers/amps❤
Thank you!
Pyrex may be confusing. Not every pyrex materials is heat resistant. Has to be borosilicate container.
imho and maybe and all due respect and thank you for the video
Isolation of microbes: a mixed culture can be inoculated on the surface of the media about 1/3, 1/4 of . After that the inoculation loop is sterilized and from the inoculated surface using one streak the microbes can be carried to other, sterile side of the media surface in the petri dish. This produces a dilutional effect and the microbial growth shows up on the diluted surfaces a single colonies making it possible to transfer colonies of a single organism to another petri dish. Mixture of microbes can be isolated into colonies grown of single, one kind of microbes. Pure colonies can be obtained this way. imho and maybe and all due respect and thank you for the video
isn't 1ml of 3% h2o2 in 100ml going to be 0,03% instead of 0,3% ?
Oh man, yes, you're correct. That's an embarrassing and misleading mistake. 0.01%-0.05% is the correct dilution, 0.3% is way too much! I can't believe I didn't catch that. I'll post a correction in the description, thank you.
what is reason for not using chemical agar just because it over processed.