How to Make Agar Media and Pour Petri Dishes for Mushroom Cultivation
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- Опубліковано 22 тра 2024
- Once you’ve mastered the basics of DIY mushroom cultivation and you’re ready to take a deep dive into the heart of mycology, making your own agar media and petri dishes is a must.
In this in-depth video, you’ll discover the secret recipe to unlock infinite nutritious growth media for your fungus. Learn how to prepare, sterilize, pour, catalogue and store your very own petri dishes. Pick up lots of great tips and tricks along the way, including how to make hydrogen peroxide media (a great alternative to antibiotic agar), how to avoid senescence, get the most out of your mycelium and keep your fungus well-fed and happy for years to come.
Once you’ve tackled the techniques in this video, you’ll be ready to charge ahead into the uncharted territory of advanced DIY mycology, including breeding, cloning and culture preservation. Next, check out our tutorial on sterile culture technique, Mycology Lab 101: • Mycology Lab 101: Agar...
Made with love by Fungaia, a heart-centered, donation-based mushroom company:
www.fungaia.life/
For the best, most reliable and super affordable mushroom
genetics and liquid culture, visit our favorite culture library:
True Blue Genetics
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Discount code: BLAGAR
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Supplies List
1. Mushroom Genetics
Gourmet and Medicinal Mushroom Cultures from Fungaia
www.fungaia.life/shop/species/
The best liquid culture on the market:
True Blue Genetics
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This is the last genetics provider you will ever need!
2. Ingredients
Organic Gelidium Agar & Media Premix
www.fungaia.life/shop/agar/
Malt Extract: amzn.to/3IX8LqA
Nutritional Yeast: amzn.to/3PD7sRa
Gypsum: amzn.to/3TBzyxt
Activated Charcoal: amzn.to/3TVTxIP
3. Petri Dishes
Sterile, Disposable Petri Dishes
Pack of 25: amzn.to/3IU0pQD
Case of 500: amzn.to/3PEVpmp
Pyrex Petri Dishes
amzn.to/4axywtq
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Recipes
MEA
10 grams agar
10 grams malt extract
pinch each gypsum & yeast
500 mL water
PDA
10 grams agar
8 grams dextrose
3 grams potato flakes
pinch each gypsum & yeast
500 mL water
VIDEO CORRECTION!
The correct dilution for hydrogen peroxide is 0.03%, not 0.3% as said in the video. The measurement shown is correct, 1 ml 3% H2O2 into 100 mL agar. Sorry!
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Other Useful Supplies
The Fungaia Cube Kit
www.fungaia.life/shop/kits/?q...
An organic, all-in-one grow kit for dung-lover mushrooms
Pre-Sterilized Grain Bags
www.fungaia.life/shop/fertile...
Pasteurized, Manure-Based Mushroom Substrate
www.fungaia.life/shop/fertile...
Monotubs and monotub filters: Microppose
microppose.com/r?id=h776jn
Discount code FUNGAIA
Bulk Liquid Culture IV Bags
www.fungaia.life/shop/spawn/?...
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More Videos from Fungaia
Mycology Lab 101: Sterile Culture Technique
• Mycology Lab 101: Agar...
How to Grow Mushrooms the Easy Way:
• How to Grow Mushrooms ...
How to Make Grain Spawn:
• How to Make Grain Spaw...
How to make your own liquid culture
• How to Make Liquid Cul...
Advanced liquid culture method
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How to make your own injection port jar lids:
• How to Make Injection ...
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Upcoming videos:
Introduction to Mushroom Cultivation
The Basics of Breeding
Storing Genetics and Managing a Mushroom Culture Library
Troubleshooting Contamination: Identification, Prevention and Problem-Solving
Working with Mushroom Grow Bags
Making Substrate for Dung-Loving Mushrooms
Making Substrate for Wood-Loving Mushrooms
Building a Home Lab
The Laminar Flow Hood
The Fungaia Flow Hood Kit
DIY Bulk Pasteurizer
DIY Cold Storage
DIY Water Filtration
Tyndallization: How to Make Grain Spawn without a Pressure Cooker
Low-Tech Mushroom Cultivation
Outdoor Mushroom Cultivation
The Liquid Culture Revolution: 5 Ways Liquid Culture Will Change the World
Fungaia's Bulk Liquid Culture System
R.E.M.I.S.: The Remarkably Efficient Mass Inoculation System
Myco-Materials
Created by Paul Lynn. All rights reserved.
(Made using some free stock footage.)
© 2024 Alembic Enterprises LLC
Walla Walla, Washington
0:00 Intro
3:18 Overview
3:35 Background
5:48 Side Note: No-Pour Agar Tek
6:26 Supplies
10:48 Ingredients
17:49 Recipes
23:05 Part 2: Prep & Sterilization
26:12 Part 3: Pouring Plates
29:14 Pro Tip #1: Color Coding
29:47 Pro Tip #2: Hydrogen Peroxide Plates
31:38 Drying
32:06 Pro Tip #3: Collating
32:34 Storage
33:44 Cleanup
34:08 Outro - Наука та технологія
This is a criminally underrated channel! I love it when our dude does the pirate and other character impressions like the professor. lol
This dude is the new, cooler Bill Nye the Science Guy. I'm a big fan.
This is excellent content for getting youth involved in science of mycology, like Bill Nye did for science generally when I was a kid.
No words to express how much your videos are exactly the perfect content for an absolute beginner and also for someone who has been working with mushrooms for years. Thank you for your enthusiastic content.
This is easily one of the best agar videos on YT. Great explanation, entertaining, and very useful tips.
Great video Paul, I've seen this and the mycology lab 101, and it has been some of the best long-form content on this website as of late. The quality of information and production is greater than quite a few of my university professors (albeit embarrassing on their behalf). Thank you!
Dude has character and extremely useful information I have not found elsewhere. AAAARGGGHHHHH
You mean like a SpongeBob knock off pirate? "Aree you ready kidsssss"... Yeah we see it.
@@DeathFromAbove_5.56 You do realize sheomery is technically out dated due to the drop in trust as well as pros still using that forum? Was a good run but it's been used to arrest ppl in the past so nah bro. Stop glowing.
looking forward for your diy laminar flow hood video. its really expensive to ship your stuff to EU. love the videos tho, keep it up!
Excellent timing! Thank you for your generosity- I ordered 1 starter syringe of oyster & received 3 extras!!! Wee! You are awesome! Thank you thank you! 🙏🏻 I have been slightly intimidated by the agar process but have my Petri dishes standing by & hopefully after your video will gain the confidence to begin. Mush Appreciation for your work & beautiful Spirit, Paul! ❤️❤️❤️❤️❤️❤️❤️❤️❤️
Love the content. It's very informative and quite enjoyable to watch. I've already learnt a bunch.
This was exactly what i needed this morning, you are a legend!
Sweet! Just started in mycology and I love learning all these different agar recipes! I can’t wait to watch it!
We have to be careful relying on 20 year old growing advice when we've advanced the hobby beyond that point. I mean look it up, peroxide is toxic to mycellium....homeboy is a rookie. Wallax2 doesn't want him here. GO BACK TO PORTLAND @FUNGIA
This is the best free mushroom cultivation educational channel on the internet! Thank you so much Paul from Caracas Venezuela!
😂this dude is what I would say should be the face of the myco community… he’s not afraid to let his weird shine through and he understands the pain and the hurdles of starting out as a young mycologist dude I started crying when you said shoplifting flats of mason jars😂😂😂 you are a legend and when I die I want you to know that it may not be a lot but I have taken my wife out of my will and replaced her name with you…fr
Amazing video on DIY Agar plates!! Thank you for sharing ❤
Thanks Professor. I really enjoy your videos and all your great info. You’re a true pleasure to watch. Looking forward to more great videos.
Media flask with pouring ring is king 💪
I like you Channel very much, as im currently starting to grow my own shroomies. I like the way you explain things. You are a very likable guy.
Great video as always! Thank you for giving us the directions and the confidence to explore while being fun and interesting. As someone who is new to this I can't understand why you don't have followers in the millions? Thanks again!!
Love your channel. Reinspiring me as a grower.
Awesome stuff! I look forward to more content.
Wow those are big speakers! Fungi Rock!
I learned alot! Thankyou.
Nicely done sir!
Just received my order and I've never seen mycelium so thick and healthy in a syringe 😮🎉 100% killing it
Appreciate the information! ❤
At 20:00 your adding lots of nutrients. This may have an adverse effect on your growth due to the ability of bad bacteria or fungus to thrive eating those same nutrients. Sometimes less is more. It's usually a good precedent to test this out in smaller batches and get your choice of culture stable prior to only using this recipe.
Always have a backup plate ready in case things go wrong. Good luck everyone and learn before you burn. Mycology is awesome 👍😎
Thank you!
Fascinating. When I first got in to growing mushrooms I didn't realize that I'd have to become a micro guy! I just started, I injected spores into my substrate x4 and nothing happened. Great first failure LOL.
Like the videos. Subscribed. 👍
I love that you ate the agar😅
Please keep making videos
He is the king👑🔥🌹
I made a mesh table for my SAB
And I made it a little slanted
I have been pooring plates slanted for months and I love it
upload more please
Thank you for the video.
I think, regular jello should work too. Regular gelatin melts at 38 deg C in an incubator.
That temperature is used for bacterial growth copying human body temperature. Fungal cultures are incubated, usually, at room temperature 18 deg C to 20 dec C. Gelatin stays jello.
imho and maybe and all due respect and thank you for the video
Isolation of microbes: a mixed culture can be inoculated on the surface of the media about 1/3, 1/4 of . After that the inoculation loop is sterilized and from the inoculated surface using one streak the microbes can be carried to other, sterile side of the media surface in the petri dish. This produces a dilutional effect and the microbial growth shows up on the diluted surfaces a single colonies making it possible to transfer colonies of a single organism to another petri dish. Mixture of microbes can be isolated into colonies grown of single, one kind of microbes. Pure colonies can be obtained this way. imho and maybe and all due respect and thank you for the video
Hi i have the 8qt Instant Pot Pro & wondering how long on HIGH to sterilize Agar & brf jars?
@_fungaia
How do you prevent it from the agar from cracking once it is poured and set to dry? I noticed some plated when I go to bag them, some of the agar inside the petri dishes are cracked so to speak.
imho and maybe and all due respect and thank you for the video
The video shows the petri dishes held upside down. The larger dish should be lower and the agar should coat the smaller dish the way that the ceiling of the smaller dish is coated. This way microbes cannot fall on the surface of the agar to avoid contamination. This is routinely done in microbiology laboratories. Petri dishes with microbiological medias are routinely stored like this before inoculation during storage of sterile condition, and after inoculation during incubation.
Mycological specimen are usually incubated on room temperature (18 -20 dec C) bacterial media is incubated at human body temperature (38-40 dec C). At 38 deg C gelatin melts, agar-agar stays solid.
Gelatin media is probably useful for mycology specimen, because it stays solid at 20 deg C incubation.
Obtaining gelatin, jello, is easier in an everyday setting, and it is useful for incubation on petri dishes.
It may be a kind of biiohack.
anyone have any insight on putting mycelium from a pf cake on an agar plate. It should work to clone right? I'm going to try no matter what just wondering what some of you think.
Ty, this is great. I might have missed it, but... what if I have agar leftover? Can I store it for later and reheat it and pour more petris?
Yes. You can but keep in mind each exposure to open air will possibly introduce contamination. It also may be a bit clumpy depending on your recipe and age of agar.
@@TheTubejunky Thanks 🤙
What would you recommend for Lion's Mane agar? Thank you
Any of the recipes mentioned work great for Lion's Mane!
@@_fungaia thank you, oh wise blagar pirate 🙇🏻
Here's a SUPER THANKS! You are the best!
Pyrex may be confusing. Not every pyrex materials is heat resistant. Has to be borosilicate container.
Are you using chlorinated tap water for your agar?
I use grafting tape instead of the specialized tape. I guess it's only an issue if you do no-pour agar?
As a retired lab-rat turned mycologist, I've always used parafilm until recently. I switched to grafting tape and find it is much cheaper (0.4 cents per plate vs. 10 cents per plate for parafilm) and much faster since I don't have to cut the grafting tape into strips like I did for parafilm. Takes a little getting used to if you're already familiar with parafilm but well worth learning.
Since grafting tape is so cheap I do seal every plate after the agar is set before storing. Something I didn't do with parafilm.
Thank you! Yeah I have so very much to learn.@@dthomas021
One day I shall use this information to make antibiotics once society has broken down
What type of alcohol is used In the little lamp?
Denatured I think?
wait, no parafilm?
What's the name of the intro song? ❤
isn't 1ml of 3% h2o2 in 100ml going to be 0,03% instead of 0,3% ?
Oh man, yes, you're correct. That's an embarrassing and misleading mistake. 0.01%-0.05% is the correct dilution, 0.3% is way too much! I can't believe I didn't catch that. I'll post a correction in the description, thank you.
Hey who ordered the poo poo platta
How to make agar? Well, you just link those sugars in a very specific way, et voila
You're joking. Your comment is intentionally misleading.
(The gelling agent in the agar is a polysaccharide, or two polysaccharides, formed from a sugar, (galactose?) linked in long chains, I think? You have some knowledge of biology.)
(Agar may be found in oriental food stores, or certain health food shops. It is only part of the culture medium mixture.)
appreciate every piece of your edu work, and the true blue ad made me laugh out loud fr. uphill both ways, with disgusting culture at both ends? too close to home matey
for anyone who hasn't tried true blue's genetics yet - grab the discount and do it. not a shill but they're zero bullshit, with super fast shipping, sweet customer service if there's ever an issue, and zero misses on quality yet. I think I'm four or five orders in, tried well over 10 strains, and every one of them has outperformed competitor options, whether I liked it or not.
Thank you Cpt. Blagarrr! You Rock!
What is best?
Maybe I DO want to stay in a dark room and eat mountain dew my entire life! Don't judge me!😅
Thank you!