My Favorite Recipe for Making Agar Plates Full Tutorial
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- Опубліковано 29 вер 2024
- Agar plates are very helpful in mushroom cultivation because you can test cultures for contamination by inoculating them with one drop of a spore syringe/LC syringe before attempting to use the syringe on a grow bag. If the agar plate shows clean mycelium after incubating you are good to go but if it shows other contamination growth it is best not to use the syringe. You can use certain techniques to clean up contaminated mushroom cultures while using agar which makes them necessary. They are also great for propagating cultures and storing them. I leave my agar plates on a shelf in a cold room, they are good for 6-12+ months before the thin agar dries up. For longer storage you should use agar slants: • How to Make Mushroom C...
In the past I have used other ingredients and different brands of agar and that actually caused contamination on the plates without even inoculating them. I must have wasted 100s of agar plates before figuring out it was the ingredients that was causing the problems.
As soon as I started using this recipe in this video, every plate is perfect every time.
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If you don’t have a laminar flow hood I recommend making a still air box: • How to Make a Still Ai...
Re-usable agar plate tutorial: • How to Make No-Pour Ag...
How to use the agar plates to clone mushrooms: • How to Clone Enoki & S...
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Light malt extract and agar from a health food store gives me the same results at a quarter of the cost.
That’s cool, this is my favorite recipe.
What ratio of extract and agar do you use?
I believe I’ve seen you do this with equal parts LME. Any reason for leaving it out? not necessary? I still use that recipe and works pretty well, just annoying when some plates have some condensation on the top but I’ll look in to gettin a thermometer like that.
Yeah the added LME is unnecessary because it already has enough nutrients. You can try storing the plates upside down to absorb condensation or use a heating pad on top of the plates for a few minutes.
@@SporenSprout I tried the heating pad after seeing one of your videos a while back. Thanks again for all your advice. I’m producing 80+ pounds of mushrooms a week and I can thank your content for getting me started
So i dont even need to add lme like everyone else says ?? Thanks. keep up the good work.
Right! Everything is in one bottle making it easy!
What kind of nutrients are there in the agar?
They do not say what the nutrients are but whatever it is works best for me. Probably light malt extract or dextrose and a superior scientific grade agar powder.
@@SporenSprout that's so cool. I have never seen this before 👍using the one made from Spanish seaweed and had no idea about different ones
what happens to the condensation ...... i see condensation existing while you wrap with parafilm, then how does it evaporate ..... doesnt it settle down on the agar ?
I store the plates in a warm room and the moisture is absorbed back into the plates and there is no more condensation
I am wondering how you get condensation to go away.I pour mine at 120 same way you do and always am left with condensation on the first few plates on top.By the way love your channel.
Thanks for watching! 🍄 There’s condensation right after pouring but it goes away completely for me after 1-2 days. You can put a heating pad on the top of the agar plate for a few minutes to clear it up. What temperature are you storing the plates at right after making them?
In the bio lab we used to store the agar plates with the agar up so the agar would reabsorb any moisture that condensed.
@@sterher That is great advice! I have seen others do the same thing.
@@sterher: That’s what we always did too. Even after we had inoculated plates, They were always stored upside down.
COOL, but this is Exactly the same as if i go BUY WHOLE STACK OF ALREADY LOADED AGAR PLATES FROM THE LOCAL " CHEMICAL STORE " i understand we talking about PURIFICATION AND CHEMICALS , BUT YOU DONT MIX THE CHEMICALS YOURSELF , YOU JUST BUY ALREADY PRE-MIXED MIXTURE
@@SomeOneHigh That’s why I have more videos you could watch:
ua-cam.com/video/TIFsUUg2yHQ/v-deo.html
@@SporenSprout will try to explore all your videos 👍❤️
Would micropore tape/ paper tape work here or it must be parafilm ?
Yes you can use micropore tape! Thanks for mentioning it 🍄
Good stuff...going to try that agar powder a try. I've used consumer grade ingredients for years but recently have seen some odd results. Maybe the explosion in agar related hobbies has put pressure on the supply chain. Love those PP5 plates and recently started using the new autoclavable clear plastic plates. They are a traditional Petri dish style that do fine in the PC. Being crystal clear and 90mm, they make contam cleanup and genetic work a bit easier than the frosted pp5 plates. Thanks for the quality content.
What clear pp5 plates 🧫 do you use? I've been using the bead containers but the screw on top kind be a hassle when trying to pour quickly.
Good looks on plugging the nutrient mix (evivva) 💯 just came here from your original mix vid 📝 I like the Tito bottle style 😂
Thanks for the video and clear instructions. Love watch videos with different recipes and then experiment.
This isn't to do with the video up. I'm wanting some advice can I add an casing layer after the tub has inoculated or am I to late. Kind of an emergency 🙏 I thought you would be the best on here to ask on here
You add the casing after the substrate is fully colonized, but before pinning or exposing to fruiting conditions. Once pinning starts, don't attempt to add a casing layer.
It's best to pasteurize a casing layer.
@SporenSprout That's great thanks for the quick reply and advice 🙏🍄 Mush Love
Much love! WTF happened to 90? We have a great science based slash educational community here! did he just bail or did they kick him off? There are so many great amateur mycologists that have figured out and simplified growing to a degree I never thought possible twenty years ago! You, 90, PGT, along with many others should sell some digital courses so we can keep a digital library???? Not talking coherently but would buy a memory stick with your video library along with many others! Most people stock silly items for the zombie apocalypse, give me knowledge, seeds and spores, I will be fine!
Yeah he was kicked off UA-cam unfortunately. Storing my videos on memory sticks is a great idea! I’ll have to look into that.
Amen brother!!I am the same way and I feel the same way as well. I love growing things and I love learning!!
Why not just reuse glass Petri dishes ?
Feel free to use whatever container you want
hey man do you think id be able to use gelatin powder and beef broth to make homemade agar plates as theres no agar powder here
Yes that should work
Solid technique 🌞🌙
@@SevenRemedies Thanks for watching!
Fyi. Discord link doesn't work
Thanks, should be fixed now hopefully.
Love your videos thank you.
Thank you for watching! 🍄
How is insemination done? I don't know how to do it, please help me
ua-cam.com/video/EARNzNHAzf0/v-deo.html
If you increase the size of the recipe, say by 50%, do you also have to increase the PC time?
No 45 minutes is good
@@SporenSprout Thank you!
Your Discord Link is invalid btw
Thanks for letting me know, should be fixed now.
so you mix only nutrient agar powder with water ? no need to add malt extract , dextrose or any other stuff ?
Yes that’s right!
thats some new dimension thing 🤩and mycelium grows well on it ? @@SporenSprout
Please add Turkish subtitles bro