Sandwich ELISA | Basics Explained
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- Опубліковано 10 лют 2020
- In this video we discuss Sandwich ELISA in Hindi, ELISA Basics and ELISA Test procedure as well.
Sandwich ELISA typically require the use of matched antibody pairs, where each antibody is specific for a different, non-overlapping part (epitope) of the antigen molecule. A first antibody (known as capture antibody) is coated to the wells. The sample solution is then added to the well. A second antibody (known as detection antibody) follows this step in order to measure the concentration of the sample.
Hope you find this video helpful. In case you have any doubts please feel free to ask in comments.
I keep checking comments and reply to them.
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Nice explanation. You have mentioned epitope simply as they are present at the surface of pathogen. That's for sure it's a part of antigen. But I think more precisely it should be defined as the part of a antigen that binds to paratope of an antibody. Paratope is the part of Fab region of antibody. More precisely the Complementarity Determining Regions i.e. CDRs.
But yeah it's a very nice explanation.
Arti mam, your reading skill are just amazing
Your teaching skills is amazing no needs to read again 🤗
Thanx mam we always wait for ur video
Vey Nice and detailed explanation.
Thanks from Bangladesh 🇧🇩
Excellent. ....
Thankuu mam bahut help hoti h apke videos se 😘😘😘😘😘😘😘😘
best way to explain..thanks mam
😇😇😍😍😍😍very very nice class ji…… thanks a lot 🥰🥰🥰
Awesome lecture 👍👍
Thank u maam ☺️😊 plz maam also make a video on super critical fluid extraction technique
Perfect explaination #livebiochemistry
So amazing vedio mam
Super explanation 👌
Thanks man
Nice explained mam👌
Nice explanation ma'am. I have a question like for negative case you said that before adding substrate we do washing step but if the capture antibody and secondary antibody has bound to antigen but we don't know that as solution is transparent so at that time we also do washing or not like you didn't mentioned washing step in positive case before adding substrate like logically we should do washing. Can you please explain this?
Very very very nice
Explanation is very nice Ma'am.
Thanks 😊
Thank you mam 👍👍👍
excellent
thank you ma'am
Tq mam for ur brife explanation
👍🏻👍🏻
thanks mam
Indirect sandwich elisa can use detection of both antigen & antibody like in elisa hiv 4tg gen kit
Thank you mam a very wonderful lecture and tell me where the video of competitive elisa
Thnks alot mam 🙏🙏🙏
Ma'am indirect ELISA is used only for antibody detection or can be used for antogen detection as well ?????
Thank u mam.clear ho gya
Thank you Mamta
Very nice mem 👍
Thanks mam
Thanks 👍
Welcome 👍
Can u plzz upload video related to ...how ELISA technique is used to find plant disease with example
tq
❤
Mam what is difference between direct and sandwich ELISA? Beacuse both are used for detection of antigens
Great mam. Can you do a video on EIS bio-sensing
Plz make a video on competitive elisa.
If once the antigen is bind to the the capture antibody why we add detection antibody?
appreciable (y)
Not understood
Mam I have a question, so what we can do is we can link enzyme with the capture Abs only why to complicate the whole process? Then also the color reaction can happen as enzyme will be linkedin to the capture antibody only.
Maam can u plz provide videos explaing RIA and PCR ..as well as other topucs of biotech!! ..u teach very nice
Good morning ma'am, kindly make a video on- PhD or BEd? after post graduation.
Radio immuno assay wali video ka link mil sakta h ky plz
Thank you Mam , my background not science but mai life sciences company me job karta hu bahot hi ache tarike se aap ne samjhaya thank you again
In which company you work??
Kya job hai apki??
♥️madam.....but I have a doubt...in sandwich elisa.the E-linked antibody bind to primary. Antibody not dorctly to the antigen
Mam pls make a vdo that how can we calcut bacterial growth genecn tm
Mam then just symptoms s recognize ni kr skty bcz there was no antigen???
🙏👍👌
Mam supposed antigen have only one binding site means single epitope then how other antibody can bind to that antigen?
Generally an antigen has several or many different epitopes
Ham wishing ke liye PBS buffer he kyu use krte hai
Mam Mac elisa keya hote hee??
Light data analysis jo ki direct indirect and sandwich ka hota h apne bola tha end me bataogi aap...but end me ni h ye topic
Ma'am just spose yadi corona ke antigen ko detect krna h but eske lia to capture antibody nhi h to hum elisa tech.use nhi kar skte
We can use direct elisa
@@BaaYo tq ma'am
Maam ...aap "RIA" k bare mein video nahi banaya hai kya?
Mam how can we know that which type of elisa has been performed
Chutya
Indirect aur sandwich Mai kya difference hai?
You explained in previous video indirect alisa started from antigen coating..........then here you are saying use capture antibody......is it important ?
Man western blotting pr bhi videos banana plz
western blotting per already video hai...search in playlist
What type of ELISA we will use if we want to determine the presence of apoptotic proteins???
Sandwich
Mam pdf milega kya elisa test ka?
Hello mam...
In this process..wash with water or other ?
PBS buffer
Ma'am ek comparative video Bana dijiye achha rahega
Sure, will try to make it.
Mam good morning
Mere pass question papers hai
Biotechnology ki
Aap in topics PR video banaiye dijiye mam
Mam please provides notes also for sessional exams
Mam which solution is used for washing ??
Phosphate Buffer Saline(PBS) pH:7.2
Ma'am application of ELISA explain kar de kindly.
Hlo diii i need help from you ...actually i am biotechnology 2nd year soo their are 2 subjects first is molecular biology and second is immunology or immunotechnology .....these 2 subject i didn't understand it ....plss make video on them ur suggest me someone channel on UA-cam or unacademy... and i am also following you on unacademy
Sry i forget to tell...that bsc biotehnology 2nd year
I will try to cover..starting new video series
Thanku soo much dii
In case of indirect detection of sandwich ELISA, the primary antibody used is derived from the patient or designed ?
The antibodies used in either direct & indirect sandwich method are genetically modified for particular antigen, Also this method is for antigen detection so only antigens are taken from patient's serum not antibodies.
Ma'am why you not make new videos
Mam why do we use capture antibodies ? Antigen toh enzyme linked detection antibody se bind karega hi baad mein.... So why capture antibody at first step? With respect, clear my doubt
Ma'am, could you please help me in finding biotech jobs in companies?
😂😂😂😂😂
Can upsc
Mam usko sandwich kyu bola gya h
Indirect ELISA not clear
kindly provide more simplyfy
Can you make video on reverse ELISA
Thanks mam