you're amazing! I studied my last exam from your videos and I got 17 out of 20, the teacher marked my down 3 marks for 3 questions that I didn't answer correctly because simply you didn't mention it in your videos, all thanks to you I wouldn't pass the test
Love it. Thank you so much for being a kind person and explaining things for others to understand and not to show off how much smarter you are and rest so stupid. It is a rare quality in teachers to actually want to teach.
Thank you for this very informative video. There is one thing I noticed though: the image on the bottom right shows the green detection antibody binding to the primary antibody with its Fc region, when actually it would bind with its Fab region. The Fab region is what gives the antibody its specificity against a certain antigen, whereas the Fc region is necessary for recognition by immune cells. Also, primary antibodies binding to two epitopes (antigens) simultaneously is dependent on the size of the epitope and (especially for IgG antibodies) not quite common. I hope I didn't create any confusion. Cheers
Yeah I was super confused by his drawing, thanks for clarifying! Also would each of the enzyme linked antibodies bind to two separate antibodies of interest because there are two Fab domains?
Thank you from Belgium! You are very good! PCR, Plasmids, Polyclonal Antibodies, Monoclonal Antibodies, ... all those videos are amazing! I'm not an English speaker and you talk so clear I can understand :) ... Thank you !!
That took a process that I was having difficulty understanding and made it totally understandable. Thank you so much for this and your Western Blotting video!!!!!!! My GPA thanks you as well!
Great lecture series 👍. Very clear. A minor point, in the indirect Elisa, the orientation of the enzyme linked Ab should be the same as that in the sandwich assay. It may be upside down.
Is it possible that when we 'wash' the wells, there is a chance that the bond between antigen-antibody or antibody-antibody w/ enzyme, break apart, giving us a false negative?
can the manufuctured antibody, with enzyme(green in n particular) cause change in colour incase a substrate is added to it, just in case the antibody is in its reagent bottle alone?
Excuse me, may I ask some question, if for direct detection and direct adsorption of the antigen on the wall of ELISA we still test for the presence of antibody in the blood serum or we are looking for the presence of antigen?
In terms of Indirect ELISA, how does the enzyme linked antibody bind with the antibody that is connected to the antigen? Are these antibodies able to bind through their Fc regions?
Great explanation of the process, but it would have been better if u added more informations about the Enzyme-linked-Antibody and the relation between the substrats catalyzation and the bond between the 2 antibodies. thank you
Just another big thank you from a biochemistry major for all your hard work! You're a talented teacher.
you're amazing! I studied my last exam from your videos and I got 17 out of 20, the teacher marked my down 3 marks for 3 questions that I didn't answer correctly because simply you didn't mention it in your videos, all thanks to you I wouldn't pass the test
Sandwich ELISA starts at 7:08
Very clear explanation and straight to the point. Thank you so much!
no worries, I worked really hard T.T.H Pham
Love it. Thank you so much for being a kind person and explaining things for others to understand and not to show off how much smarter you are and rest so stupid. It is a rare quality in teachers to actually want to teach.
Thank you for this very informative video. There is one thing I noticed though: the image on the bottom right shows the green detection antibody binding to the primary antibody with its Fc region, when actually it would bind with its Fab region.
The Fab region is what gives the antibody its specificity against a certain antigen, whereas the Fc region is necessary for recognition by immune cells.
Also, primary antibodies binding to two epitopes (antigens) simultaneously is dependent on the size of the epitope and (especially for IgG antibodies) not quite common.
I hope I didn't create any confusion. Cheers
good catch!
Yeah I was super confused by his drawing, thanks for clarifying! Also would each of the enzyme linked antibodies bind to two separate antibodies of interest because there are two Fab domains?
Lots of thanks .. you always boos our confidence. I always get excited to switch on your lectures
I appreciate this guy so much. Saving my grade.
OMG I cant thank you enough ♡
+YulYul Addict You're welcome! :)
Thank you from Belgium!
You are very good!
PCR, Plasmids, Polyclonal Antibodies, Monoclonal Antibodies, ... all those videos are amazing! I'm not an English speaker and you talk so clear I can understand :) ... Thank you !!
Sir sir sir..U r saviour of a biochemist.. Profound Respect from Pakistan...
The best explanations require no follow up questions. Thank you!
Thank you for this video! It must have taken a lot of effort to draw the board etc. very very much appreciated!
You are a living legend. Respect!
Great!! Very helpful to an Organic Chemist developing Biopharmaceuticals... God Bless You With More Energy!!!
U did such a good job in explaining this method! Thank you so much for that explanation! (Y)
Just want to say that this video is SO extremely helpful. Please post more.. you explain everything so clearly! THANK YOU!
Thank you so much! I loved the way you explained all the concepts
Thanks very much Dr, My immunology course is a always easy to understand when I listen from you!
Omg im so glad i found your channel. Thank you!
You're amazing! Thank you for making this simpler.
Absolutely beautiful explanation on Sandwich ELISA! Thank you so much, this will help me with my lab reports! 🥰🥰
That took a process that I was having difficulty understanding and made it totally understandable. Thank you so much for this and your Western Blotting video!!!!!!! My GPA thanks you as well!
You are a gift. Thank you for your awesome, clear, concise and colorful explanations on these methods. :D
no you are are gift kim.
saving my gpa like always
thank you so much
Thank you for uploading this! Very concise and helpful!
I love your videos. They are so clearly explained. Thank you.
Wow you are such a good explainer and teacher.... Keep up the good work... Kudos to your explanation
So clear and concise! Thank you!!!
Thank you very much for this explicit lesson!
Very clear! I didn't understand the Sandwich Elisa really well, but now I do! Thanks!
This video is just what i needed! thank you
Great lecture series 👍. Very clear. A minor point, in the indirect Elisa, the orientation of the enzyme linked Ab should be the same as that in the sandwich assay. It may be upside down.
thank you so much all the other videos I watched were so confusing but you made it so understandable!
These videos are great! You deserve more views and likes
Brilliant!! Thank you. So clear.
You're seriously underrated! Thank you so much!
the opinion of the scientific community is that he is appropriately rated
the best explanation ever! thank you
AK lectures are the best....best explanation to every topic...thank you for making such helpful videos...can't thank you enough😊
Thank you so much for this clear explanation!
Why are you such a legend?
Killed it bro! Thanks !
Thank you for your help!!
Thank you so much for your videos!
i love your lectures so much thank you!!!!
Excellent. For medical students this is a Godsend
Awesome explanation!
you've helped me very much ,, thank you
THANK YOU , THANK YOU , THANK YOU!!! GOD BLESS YOU!!
Excellent lecturer!
So good! Thank you so much!!!!
thanks for the explanation helpd me alot !!
Is it possible that when we 'wash' the wells, there is a chance that the bond between antigen-antibody or antibody-antibody w/ enzyme, break apart, giving us a false negative?
I finally got it right! Thanks!
Great Job!
I love you. You're videos are so helpful. I wish i could just take your class
he might not be able to wear that sexy athletic-cut v-neck in his class though, so you may be better off!!
one of the best Videos
Watch this on the day of your exam….I see you!
Staying up late trying to understand this
Keep going!
Thank you!
Very clear , thank you a lot !
how is the first antigen or antibody we added at the first not removed when we wash off after each step ??
Thanks a lot! You are the BEST ever! I just donated:-)
thanks a lot , good explanation ! ^^
You are the best! Thank you sooooooo much
Thank you very much, so well explained and understandable.
Thank you so much for this very informative video :)
Thank you so much for this perfect explanation
Sir, YOU ARE AMAZING, thank you so much! :)
thanksss ! very useful and detail explanation
thank you !
Really helpful, thank you :)
very helpful and clear as usual one question though I'm not seeing Direct and Competitive ELIZA did I miss it?
Thanks a lot.. Very very helpful..❤
Wow ! This was very informative thank you
Just continue
Thank you so much
O my Goa! Really you are agreat lecturer!!!
can the manufuctured antibody, with enzyme(green in n particular) cause change in colour incase a substrate is added to it, just in case the antibody is in its reagent bottle alone?
Thank you so much!!!!
great video
nice explanation.....thank you soo much
You are the best really🦋
Excuse me, may I ask some question, if for direct detection and direct adsorption of the antigen on the wall of ELISA we still test for the presence of antibody in the blood serum or we are looking for the presence of antigen?
Amazing explanation
Thank u so so muchhh👏🏼
In terms of Indirect ELISA, how does the enzyme linked antibody bind with the antibody that is connected to the antigen? Are these antibodies able to bind through their Fc regions?
Thank you ❤
Wow, super helpful.
Great explanation now it’s clicking
Very clear explanation ☺
you are amazing, thaaaaaankkkk you
ربنا يكرمك يا حاج والله 😂❤❤❤
THANK YOU!!!!!!!!!!
Thank you so much it helped me for exams
this guy rocks
Great explanation of the process, but it would have been better if u added more informations about the Enzyme-linked-Antibody and the relation between the substrats catalyzation and the bond between the 2 antibodies.
thank you
Great thanks
i love you AK lectures
thanks be to AK lecture ,sir you forgot to add stop solution especially HCL acid .but actually explanation was very fine.
thank you sir. may GOD bless u
thanks really helpful
plz do upload a lecture on competitive ELISA if possible, thanku