you're amazing! I studied my last exam from your videos and I got 17 out of 20, the teacher marked my down 3 marks for 3 questions that I didn't answer correctly because simply you didn't mention it in your videos, all thanks to you I wouldn't pass the test
Love it. Thank you so much for being a kind person and explaining things for others to understand and not to show off how much smarter you are and rest so stupid. It is a rare quality in teachers to actually want to teach.
Thank you for this very informative video. There is one thing I noticed though: the image on the bottom right shows the green detection antibody binding to the primary antibody with its Fc region, when actually it would bind with its Fab region. The Fab region is what gives the antibody its specificity against a certain antigen, whereas the Fc region is necessary for recognition by immune cells. Also, primary antibodies binding to two epitopes (antigens) simultaneously is dependent on the size of the epitope and (especially for IgG antibodies) not quite common. I hope I didn't create any confusion. Cheers
Yeah I was super confused by his drawing, thanks for clarifying! Also would each of the enzyme linked antibodies bind to two separate antibodies of interest because there are two Fab domains?
Thank you from Belgium! You are very good! PCR, Plasmids, Polyclonal Antibodies, Monoclonal Antibodies, ... all those videos are amazing! I'm not an English speaker and you talk so clear I can understand :) ... Thank you !!
That took a process that I was having difficulty understanding and made it totally understandable. Thank you so much for this and your Western Blotting video!!!!!!! My GPA thanks you as well!
Great lecture series 👍. Very clear. A minor point, in the indirect Elisa, the orientation of the enzyme linked Ab should be the same as that in the sandwich assay. It may be upside down.
Is it possible that when we 'wash' the wells, there is a chance that the bond between antigen-antibody or antibody-antibody w/ enzyme, break apart, giving us a false negative?
Great explanation of the process, but it would have been better if u added more informations about the Enzyme-linked-Antibody and the relation between the substrats catalyzation and the bond between the 2 antibodies. thank you
can the manufuctured antibody, with enzyme(green in n particular) cause change in colour incase a substrate is added to it, just in case the antibody is in its reagent bottle alone?
Excuse me, may I ask some question, if for direct detection and direct adsorption of the antigen on the wall of ELISA we still test for the presence of antibody in the blood serum or we are looking for the presence of antigen?
In terms of Indirect ELISA, how does the enzyme linked antibody bind with the antibody that is connected to the antigen? Are these antibodies able to bind through their Fc regions?
Sandwich ELISA starts at 7:08
Just another big thank you from a biochemistry major for all your hard work! You're a talented teacher.
Sir sir sir..U r saviour of a biochemist.. Profound Respect from Pakistan...
you're amazing! I studied my last exam from your videos and I got 17 out of 20, the teacher marked my down 3 marks for 3 questions that I didn't answer correctly because simply you didn't mention it in your videos, all thanks to you I wouldn't pass the test
Very clear explanation and straight to the point. Thank you so much!
no worries, I worked really hard T.T.H Pham
Love it. Thank you so much for being a kind person and explaining things for others to understand and not to show off how much smarter you are and rest so stupid. It is a rare quality in teachers to actually want to teach.
Lots of thanks .. you always boos our confidence. I always get excited to switch on your lectures
Thank you for this very informative video. There is one thing I noticed though: the image on the bottom right shows the green detection antibody binding to the primary antibody with its Fc region, when actually it would bind with its Fab region.
The Fab region is what gives the antibody its specificity against a certain antigen, whereas the Fc region is necessary for recognition by immune cells.
Also, primary antibodies binding to two epitopes (antigens) simultaneously is dependent on the size of the epitope and (especially for IgG antibodies) not quite common.
I hope I didn't create any confusion. Cheers
good catch!
Yeah I was super confused by his drawing, thanks for clarifying! Also would each of the enzyme linked antibodies bind to two separate antibodies of interest because there are two Fab domains?
Thanks very much Dr, My immunology course is a always easy to understand when I listen from you!
OMG I cant thank you enough ♡
+YulYul Addict You're welcome! :)
You are a gift. Thank you for your awesome, clear, concise and colorful explanations on these methods. :D
no you are are gift kim.
Thank you from Belgium!
You are very good!
PCR, Plasmids, Polyclonal Antibodies, Monoclonal Antibodies, ... all those videos are amazing! I'm not an English speaker and you talk so clear I can understand :) ... Thank you !!
I appreciate this guy so much. Saving my grade.
Thank you for this video! It must have taken a lot of effort to draw the board etc. very very much appreciated!
Great!! Very helpful to an Organic Chemist developing Biopharmaceuticals... God Bless You With More Energy!!!
The best explanations require no follow up questions. Thank you!
i love you AK lectures
Just want to say that this video is SO extremely helpful. Please post more.. you explain everything so clearly! THANK YOU!
I love your Teaching approach. I passed my Exams because of this online class❤❤❤❤
saving my gpa like always
thank you so much
Excellent. For medical students this is a Godsend
You are a living legend. Respect!
These videos are great! You deserve more views and likes
U did such a good job in explaining this method! Thank you so much for that explanation! (Y)
That took a process that I was having difficulty understanding and made it totally understandable. Thank you so much for this and your Western Blotting video!!!!!!! My GPA thanks you as well!
Very clear! I didn't understand the Sandwich Elisa really well, but now I do! Thanks!
I love your videos. They are so clearly explained. Thank you.
AK lectures are the best....best explanation to every topic...thank you for making such helpful videos...can't thank you enough😊
Why are you such a legend?
Absolutely beautiful explanation on Sandwich ELISA! Thank you so much, this will help me with my lab reports! 🥰🥰
Wow you are such a good explainer and teacher.... Keep up the good work... Kudos to your explanation
You're seriously underrated! Thank you so much!
the opinion of the scientific community is that he is appropriately rated
I spent the whole trying to understand this concept . just after I watched your video everything became clear ,thank you so much
Thank you so much! I loved the way you explained all the concepts
I love you. You're videos are so helpful. I wish i could just take your class
he might not be able to wear that sexy athletic-cut v-neck in his class though, so you may be better off!!
THANK YOU , THANK YOU , THANK YOU!!! GOD BLESS YOU!!
You're amazing! Thank you for making this simpler.
Omg im so glad i found your channel. Thank you!
Thank you so much for this clear explanation!
thank you so much all the other videos I watched were so confusing but you made it so understandable!
Watch this on the day of your exam….I see you!
Staying up late trying to understand this
Keep going!
Thank you for uploading this! Very concise and helpful!
Great lecture series 👍. Very clear. A minor point, in the indirect Elisa, the orientation of the enzyme linked Ab should be the same as that in the sandwich assay. It may be upside down.
Thank you so much for this perfect explanation
Thank you very much, so well explained and understandable.
U r the best 💜🇮🇶🥀
the best explanation ever! thank you
Excellent
Thank you so much it helped me for exams
So clear and concise! Thank you!!!
Thank you very much for this explicit lesson!
i love your lectures so much thank you!!!!
one of the best Videos
Awesome explanation!
Excellent lecturer!
Wow ! This was very informative thank you
Thank you for your help!!
This video is just what i needed! thank you
Thank you ❤
Jazak ALLAH sir❤️❤️❤️
Brilliant!! Thank you. So clear.
Is it possible that when we 'wash' the wells, there is a chance that the bond between antigen-antibody or antibody-antibody w/ enzyme, break apart, giving us a false negative?
Killed it bro! Thanks !
O my Goa! Really you are agreat lecturer!!!
thanks be to AK lecture ,sir you forgot to add stop solution especially HCL acid .but actually explanation was very fine.
thanks for the explanation helpd me alot !!
Thanks very much for this helpful video.
Great explanation now it’s clicking
Thank you so much for your videos!
thanksss ! very useful and detail explanation
Sir your oldest student, I passt my many tests bcz of u
You are the best! Thank you sooooooo much
I finally got it right! Thanks!
You are the best really🦋
Very clear explanation ☺
Amazing explanation
i’m taking A level biology, and this was confusing but thank you for this video!!!
taking this again in med school, and i came back to your video… thank you so much!
Thanks a lot.. Very very helpful..❤
You sir are a legend. Thanks a lot.
no, you madam are a legend
Very clear , thank you a lot !
Anyone know if all his biochem videos cover all the MCAT biochem topics? Please help!
ربنا يكرمك يا حاج والله 😂❤❤❤
Great explanation of the process, but it would have been better if u added more informations about the Enzyme-linked-Antibody and the relation between the substrats catalyzation and the bond between the 2 antibodies.
thank you
Saved me again. Now im ready for my exam tomorrow!
😂😂
you've helped me very much ,, thank you
can the manufuctured antibody, with enzyme(green in n particular) cause change in colour incase a substrate is added to it, just in case the antibody is in its reagent bottle alone?
Thanks a lot! You are the BEST ever! I just donated:-)
Excuse me, may I ask some question, if for direct detection and direct adsorption of the antigen on the wall of ELISA we still test for the presence of antibody in the blood serum or we are looking for the presence of antigen?
Great Job!
Thank you so much for this very informative video :)
thank youuuuuuu so muchhhh
In terms of Indirect ELISA, how does the enzyme linked antibody bind with the antibody that is connected to the antigen? Are these antibodies able to bind through their Fc regions?
Sir, YOU ARE AMAZING, thank you so much! :)
how is the first antigen or antibody we added at the first not removed when we wash off after each step ??
So good! Thank you so much!!!!
Just continue
Thank you so much
nice explanation.....thank you soo much
elisa for s sorbent !!! u wrote eliza .. :) luv ur videoss................thankuu
I wish that I can explain information just like you
very helpful and clear as usual one question though I'm not seeing Direct and Competitive ELIZA did I miss it?