IDvet tutorial: How to perform an ELISA test (protocol for poultry testing)

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Discover how to run an ELISA test, step by step. The procedure shown in this video is specific to avian testing. The IDvet ELISA range covers multiple species, including livestock and poultry. For more information, visit www.id-vet.com.

[@kirankahal4754]

Thank u so much for this

[@InnovativeDiagnostics]

And thank you very much for your interest!

[@hassanmahe8708]

Thank you very much very interesting

[@InnovativeDiagnostics]

Thank you very much for watching us and for this nice comment, very appreciated!

[@GaryHField]

The acronyms of the test is LAM ELISA. That’s very strange.

[@amrosalman5411]

Thanks alot

[@InnovativeDiagnostics]

You are very welcome!

[@amelkanani3570]

I want to ask...
Some well has more color (deep in color) than the positive control?

[@InnovativeDiagnostics]

Hello Amel,
With indirect ELISAs, the more a sample is positive (containing more antibodies), the stronger its color will be during the revelation step.
A sample may contain more antibodies than the positive control. Consequently, this sample will have a stronger color than the positive control.

[@divasan9281]

Which insturment are you using to wash the wells? Around the 5th minute. Thanks

[@hassanalghalebi528]

you need washer to do that

[@InnovativeDiagnostics]

Hello, sorry for the late reply. There are several possibilities to wash the wells. Please contact [e-mail] to ask for more information.

[@zeeshanraza7423]

WHEN we use different strips of plate ...negativ and positiv controlls are used at each step

[@hassanalghalebi528]

different for same diseaes or another diseases

[@InnovativeDiagnostics]

Hello, sorry for the late reply. Please send your question to [e-mail]: our technical support team will be pleased to help.

[@zahraafakih7020]

What is the internal reference material used for? Like what's the purpose..?

[@InnovativeDiagnostics]

Hello, sorry for the late reply. Please contact [e-mail]: our technical support team will be pleased to answer your questions and give you more details about the reference material.

[@NawalArsalane]

I use R18

[@nextagro]

shouldn't it have done that in a sterile conditions under a laminar flow?

[@InnovativeDiagnostics]

In most cases, ELISA testing does not have to be performed under sterile conditions or under a laminar flow.
In some countries, however, there is a regulatory requirement to perform Avian Influenza ELISA tests in level 2 or 3 containment laboratories.

[@user-kh7uy6ir4w]

Tôi thích sản phẩm này ❤❤❤

[@InnovativeDiagnostics]

Thank you so much for the nice feedback! Cảm ơn bạn rất nhiều ;)

[@dvmgeelle5520]

Lets share us calculation formula of
Wash solution and Buffer

[@InnovativeDiagnostics]

Hello, sorry for the late reply. Please feel free to contact [e-mail] to learn more about the calculation formula.

[@iammrm6]

Is this a direct ELISA?

[@InnovativeDiagnostics]

Hello, the test used in the video is an indirect ELISA.

[@sozanhassanein5641]

How to calculate limit of detection for compitative idvet pprv elisa

[@InnovativeDiagnostics]

Dear Sozan, please send your question to our technical support team, who will be pleased to assist you: [e-mail]. Wishing you a great day!

[@shivaprasad6493]

Sir hiv elisha test refar rann 0.2127 nagative positive please respond any where

[@InnovativeDiagnostics]

Hello, you may send your questions to [[e-mail]]. Our technical support team will be pleased to assist you in your result interpretation.

[@shivaprasad6493]

@@InnovativeDiagnostics contact no

[@amandabarthes6953]

@@shivaprasad6493 You will find our contact info here: www.innovative-diagnostics.com/contact-us/

[@sallymusallam2178]

I'm also in for some checkups ELISA refar rann 0.15 negative positive ! In your opinion what is the problem and what is the solution !?

[@saranomrosy7821]

Howl can I get software? I get kit but I haven't software

[@InnovativeDiagnostics]

Thank you very much for your interest. To request the software, please write to: [[e-mail]]. We will be pleased to assist you in installing and using the software.

[@saranomrosy7821]

@@InnovativeDiagnostics thanks alot because I have more than one kit but unfortunately I can't get software

[@IbrahimAhmed-ht7rc]

😄😄😄😄🌹🌹

[@moussaeshra6212]

I didn't understand any
You are talking as we know all the basics
We dont
How did you prepare the sample

[@InnovativeDiagnostics]

Thank you for your interest. This video aims to describe the main steps of a poultry indirect ELISA protocol. If you need any additional information, for example on how to prepare your samples, please feel free to contact us at [[e-mail]]. We will be pleased to assist you.

[@AndishaAlex]

Sorry, in Amharic

[@InnovativeDiagnostics]

Hello, you may activate the subtitle option in the toolbar at the bottom of the video. An automated translation is available in various languages, including Amharic.