Indirect ELISA | Basics Explained

Friends, In this lecture we discuss ELISA basics and Indirect ELISA technique. We also discuss how ELISA Test is performed and it's procedure.
ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies, and hormones.
Hope you find this video helpful. In case you have any doubts please feel free to ask in comments. I keep checking comments and reply to them.
Related Videos:
Competitive ELISA: • Competitive ELISA | Ba...
Sandwich ELISA: • Sandwich ELISA | Basic...
Direct ELISA: • Direct ELISA | Basics ...
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[@BaaYo]

To access notes and practice questions, please join our Telegram channel t.me/baayo_official

[@dr.yasirnawaz5230]

Miss arti...perfect delivery at basics. Dear, I suggest you continue for advanced levels of these techniques.

[@suvadipjana1185]

Mam You are the best teacher I've ever seen..your teaching process very easy to learn and understand. Thnk you soo much mam.. Prannam🙏 aap jahan v rahe iss pandemic main bilkul surakshit rahe..aur bohot aache rahe hamesha.

[@pankajissar2279]

You way of explaining is excellent,
Voice sounded with positive vibrations

[@VinodSharma-px8tw]

Thanks a lot mam its amazing i wants to clear this topic in evening ..

[@neetuyadav2881]

Very very nice vedio mam
Aapne sir doubts se clear kr diye
Aap UA-cam pr sabse good teacher ho
And thank you so mucch mam

[@pritamprasad9317]

Kamal ka explanation tha...itna simple or or informative thank u so much

[@ahsanalam2898]

What a creative mind mam to explain so easy way thanks mam very helpful video God bless you

[@mariaqadri2705]

Best explainer ever👍🏽✨

[@jyotirana6884]

Worth watching. Explained in better way

[@arungupta3739]

Quality content 👍

[@farmannabi5784]

Outstanding teaching...

[@vidhyaminde6110]

Amazing ❤️😍 thanku mam... world's best teacher

[@exmuslimadamsiker]

Thank you very much mam for very informative video and tell me about pcr variants

[@Isha45547]

Crystal clear concept

[@anjalijangra8102]

superb way of teaching 👍👍

[@anwaypatra556]

You are awesome, thank you so much.

[@manishasingh3536]

Ma'am you are really a wonderful teacher

[@secretgirls408]

Easiest explanation ever...👍👍

[@sureshchandbairwa3550]

Wow... excellent

[@seemabaheshwar5875]

Thank you so much mem.. Aap bahut achhe se explain karte ho.

[@vermasona2820]

Thankuu youu mam..youu make this topic easy♥️

[@lifemotivation2161]

Woww,, nice explanation 👍thanx

[@fazilfayaz6557]

💥....
Great

[@_DISHARATHOD_CMB_FY]

Well explained 🙏🎉

[@dr.p.shivakumarzoology896]

Thank you very much Very useful video

[@prajjwalpaudel4653]

thanks man.... clear cut teaching... very easy to understand..

[@BaaYo]

Thanks Prajjwal

[@livebiochemistry]

V nice...now waiting for sandwich Elisa..#livebiochemistry

[@preetigautam8405]

Thank so much mam .... App bahut hi achha padhati h means bahot hi achha

[@misbashabir6514]

Thanks soo mch mam... Easy to understand ..... ❤from kashmir..

[@nikhilmalhan5843]

Crystal clear mam ☺

[@ramchandrasuthar2848]

Nice analysis mam

[@nursem.p.abhilashatekam7095]

Well done💯👍👍👍

[@Gargi12334]

Ur way of teaching is best mam 👏👏

[@BaaYo]

Thank you Vidhi..vidhi please share these videos with your friends and classmates

[@getitdone0010]

Yaar thank you so much....

[@rajuharipuria4706]

Very helpful ma'am🙏

[@sarveshsingh1193]

Thanks,ma'am

[@farmannabi5784]

Respect and love from Pakistan...

[@PankajGupta-ij5mr]

Very nice video 🔥🔥

[@sanjeevswarnkar753]

Nice explanation

[@peshawarzalmi7781]

Tnx alot 💝Mam

[@AnshikaSingh-jh6mj]

Thank you mam ❤

[@shardasuthar3548]

Good classes mm

[@gulamsbrajpot6580]

Ur way of teaching is more gd

[@ramveeryadav5647]

Nice teaching

[@amitsarkar7215]

Very nice

[@RajeshKumar-ui7pe]

Thanks a lot mam

[@sumanaparvin8196]

Thank you mam ..

[@raavirangmunch]

👌🏼👌🏼

[@AyeshaKhan-ce3zj]

Mam awesome !

[@dakshapatel2455]

Can u plzz upload video related to ...how ELISA technique is used to find plant disease with example

[@parmaryagnik7712]

Your videos halp me pass my exam thanks mam

[@vvlog20]

Helpful thanks mam

[@lvi_gaming_yt2017]

🔥🔥

[@dr.pravinsinhchauhan8725]

very informative..i want to learn the how to analyse data by Elisa reader.. if any ppr please share

[@jahangirraju5333]

thanks dear

[@bushranaqvi9779]

veryyyyy well

[@mamtaom6098]

👍

[@nemkumarsahu8050]

Thanks mam

[@sunadbalgi1669]

Amazing video😁

[@BaaYo]

Thanks

[@ManiSharma-dn2rm]

Nice video ma'am

[@ruhuljalil]

Ma'am please explain Recombinant DNA technology in full details. With all chemicals and enzymes used in it. Thank you Ma'am for explaining us in so simple language. God bless you.

[@BaaYo]

Ruhul Some parts i have covered..remaining i will cover soon..

[@ruhuljalil]

@@BaaYo Thank you Ma'am

[@AdityaRaj-zk9eh]

thanks mam

[@rajthacker8329]

Mam in all the specific microtiter plate same blocking agent is used ie. BSA ?

[@shailidevi4085]

Mam, is it conjugate is equal to bsa? Image based q comes in aiims pg , i m that one is indirect elisa!in q we hav to locate conjugate in that image?

[@exmuslimadamsiker]

Tell me about substrate and their reaction colour

[@rajthacker8329]

How secondary antibody can bind to primary antibody ? As antigen and antibody can interact due to epitope and paratope present on the surface . Are there any specific structure on pri and sec.. antibody

[@ginjalasaraswathi1166]

Ya same doubt

[@suryainformator421]

Why is secondary antibody used while already, primary antibody and malarial antigen are attached

[@blissfulanddevotionallife7056]

👌👍

[@sumayaislam8565]

Mam, keya a possible hai ki second antibody antigen ke sathe bind kare?????

[@renutailorshorts8155]

Ma'am please provide more video for techniques and also advanced videos

[@ptiyouthiya]

What if we add substrate and wash it and can we read that ?

[@muhammadahmadmushtaq2811]

AoA
I'm from Pakistan
Please ap jis topic ki video uploaded karti ha. Es ka relative questions b batya kara

[@durgeshsharma9896]

Hello mam
First step plate wash sabhi test ke liye rahta hai
Ye kuch test me use hota hai

[@itsallaboutpathology]

Very well explanations

[@BaaYo]

Thank you Prachi

[@BaaYo]

Prachi please share this video with your friends and classmates

[@medicaleducation8978]

Mam indirect Elisa main ham antigen ko b detect karsaktai hain.
It only increase the sensitivity of the test .?

[@snehakulthe1546]

What is used for washing?

[@KoyelsuvoVLOG]

Mam washing k liye konsa solutions use kiya jata hai?

[@manjeetkumar7143]

Wash ham kis reagent karenge ma'am

[@ashutoshnavik4818]

Hlw mam please explain all biochemical test ex- Indol, MR, VP, etc

[@dikshantsharma7494]

The antibody extracted from human body and introduced in rat act as antigen for rat and rat body start producing antibody against it and called secondary antibody.

[@meghadixit8954]

ma'am secondary Ab primary Ab se kese attach ho jati h constant region pr

[@ithirstyforknowledge]

why do we add secondary AB?

[@banotalpur9152]

I have a question that antibodies can only present in serum? Why Not in plasma ?

[@zohakhan7004]

MAM you are explain the difference btw direct and in direct elisa?

[@nimrakanwal8341]

mam is me hm media ko wash kis sy krty hy ?

[@sanjeevsoni7460]

Free radical and antioxident par video baniye mam

[@moazzammajid6140]

Question?
What's the difference between direct and indirect elisa? Only the secondary antibody binding?

[@mominatariq2823]

In direct elisa ,we do antigen identification and in indirect elisa we detect antibody.

[@krishanusamanta2085]

Mam ap na jo 2nd Entibody usu kari ha kaya o market sa cell ki ha? Ar o kaya E add hotai ha?

[@hafsanasir6398]

Okay, thank you but what would happen if a patient has the symptoms of some disease and the body is not producing the antibodies and when there is no antibody the substrate won't bind to it and we will not see the color and we call that patient a negative. What test we use to see the disease when a person's body is not making the antibodies.

[@mominatariq2823]

If disease is present antibodies will be produced obviously

[@muhammadbilalurramzan2369]

Mam direct or indirect elisa both antibodies ko detect krne k leay nh hotaa
Sandwich antigen k keay hota hein

[@himanshusharma8188]

Mam please explain radio immune assay

[@aamjehainsaan2994]

Mam Mene hbsag screening krwai thi .or ruslt Aya (0.69) Aya ..mam mtlb hbsag ki antybody bni hui hai meri body main ?.mltb main immune hu. Mujhe haptites b ki vaccination krwane ki jrort to nhi ab ?

[@poojathakur4739]

I want where I can apply for companies

[@shuru5957]

Why do we use two antibodies ma'am,? I mean, why don't we use only one antibody tagged with enzyme, like in case of direct one??

[@BaaYo]

In direct elisa we detect antigen from blood serum, here in indirect elisa we detect antibody from blood serum...agar antibodies hume known nhi hai ki patient serum me hai ta nhi..to use kaise detect kroge??? Aap socho..kuch detection method hona chahiye na..ya to hum tagged antigen use kre ya tagged antibody...tagged antigen aate nhi hai...isliye antibody detect krne ke liye tagged antibody use ki jati hai

[@BaaYo]

Isliye one antibody we are detecting with the help of another tagged antibody

[@pst_iit-delhi]

@@BaaYo Thank You I was having the same Doubt.

[@mahimatomer8408]

Nic mam

[@ChiragPatel-ih5xj]

Which instrument used in ELISA ?

[@hennabyhira4632]

When we use indirect elisa?

[@rsusmitha5946]

In blood serum both antigens and antibodies ho saktha hy na... Kaise bol sakte serum meh only antigen hy

[@satishmalviya4714]

Agent used for washing??