In reality they use all FOUR ddNTPs at the same time, e.g. ddATP, ddTTP, ddCTP, ddGTP, on each sample - so you get fragemnts that end at each letter throughout the sample..based on lengths you can tell which base is at which position.
Up to what I know (and as you can see in the video), you can run all of them in the same tube as long as each ddNtP has a different fluorescent mark. If They all have the same mark, then you need to run them in different tubes.
In reality they use all FOUR ddNTPs at the same time, e.g. ddATP, ddTTP, ddCTP, ddGTP, on each sample - so you get fragemnts that end at each letter throughout the sample..based on lengths you can tell which base is at which position.
Thank you for the wonderful teaching.
Bless you.
does anyone have a flow diagran of how to sequence dna using whole blood using the sanger method
Yea that's right mark - so they need to run all 4 rxns in 4 separate tubes to read one sequence just like that.
Up to what I know (and as you can see in the video), you can run all of them in the same tube as long as each ddNtP has a different fluorescent mark. If They all have the same mark, then you need to run them in different tubes.
ya mark just like that 4 separate tubes for each base to read one sequence :)
this video explain what i need. Hope it's true
ya okay, but it doesnt explain the whole sequencing process.