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Thin layer chromatography (TLC) principle explained
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- Опубліковано 16 сер 2024
- This lecture explains about the thin layer chromatography technique. TLC principle is well explained with example. Thin layer chromatography is the easy method to separate different types of chemical molecules from each other with the help of thin layer chromatography plate also known as the TLC plate. Thin layer chromatography separates molecules based on the differential movement rate in the tlc stationary phase which is calculated with the retention factor or Rf value.
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Thank you for watching the video lecture on the thin layer chromatography or tlc.
Thank you very much sir, I got 94 th in CSIR JRF without coaching, I followed your vedios, really God will give great blessings to you sir.
Hii I m also preparing for CSIR net can I get notes
Harvard needs to hire you as their professor! My professors in America are not as good as you! They don't explain the principles as you do! Good job!
Thank you so much for appreciating my efforts
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I was not able to understand this concept for a week, But then I met Shomu sir in this video and Voila....It is fitted in my mind the easiest way. Thank you, Sir!
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I was worried about my paper Biochemical techniques which is going to be held tomorrow... Then i came to ur channel n saw all topics related to biochemistry here.. thanku so much sir. Allah bless u
Thank you. Glad you liked my lectures
My paper is after few days.
Wow sir ur explanation is very clear and very good. U explained in such a way that I need not read it again anymore. Thank you very much 👏
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Hlo I'm following from 3yrs ...your videos was very helpful for my degree biochemistry.......now I'm graduated with 75% thank you so much sir
Thank you so much for such a simple and very informative video I have my exam on 30 and I will be able to understand all the chromatographic techniques from your lectures
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Our teacher teaches us the principles for almost 2 months but still unable to get the grasp of it making me confuse everytime. Your teaching is clear and simple and I'm very thankful since I was having a hard time, may god bless you
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i almost forget the practical that i had done in my grad. thanku to remind me.
You're welcome
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By the way, I am watching this after five years of uploading but still it is thousand times better than my professor's explanation
Thank you so much for appreciating my efforts
Thanks guru ji💞...you are the only one who helped me alot in exams since 1st sem till now🙏...
Tuje samj me aaya?
Tomorrow is my paper and i had this chromatography topic.. I was going to drop this topic.. But MAN you saved my day...Tysm❤
+Danish Drop thank you. Glad you liked my lectures. Please subscribe and share
You are amazing.... your explanation language is very easy to understand easily thank you so much
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Enjoyed your Lecture sir. Can you please make some videos related to SOS DNA repair mechanism and Centrifugation. Your Videos are great. Thanks for being a Long distance teacher.
+Harsh Oza Okay. I will keep that in my mind
Kindly share the standard Rf values of amino acids in TLC with Phenol water as the solvent medium
It would be of great help
Thanks to explain in easy language......I also explained my friends easily
You're welcome
Wonderful....well explained sir...Thank you so much sir...it is very helpful for me...Thank you...And I want to tell you that I regularly attend your videos before I start a new chapter....
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Truely very well explaned 🙏Thank you so much !!
Please make video on interferences in electrophoresis 😢🙏
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It's qualitative...technique becoz one can identify the substance presence in mixture with the help of Rf value!!😊
Thank you for the great way of explanation and the lecture
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what a explanation sir
I always watch u r video before my exam and my all concept is clear due to u r video
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Good class sir.. Ur class is really informative. Thank u
very easy wording,nice communication skills....great job,keep it up
TQ u Bhai ....... This concept of chromatography contains 30marks in our examination.........
Glad to hear that you're getting benefit from my lectures
you are simply toooo good sir.
Thank you so much for appreciating my efforts
if i were to compare polar and non polar solvent, and one of it has more spots than the other, what does it mean? how to determine if polar solvent is better than the non-polar and vice versa?
It means that the one that has more spots has more compounds
U r a great teacher😊
Thank you
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Thanks for this valuable information it is very helpful
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This is so useful . Hats off for your effort sir. With the help of your video I hope I can give my best in my seminar
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Thank you sir for your guidline
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I help my friends understand such concepts and Sir Shomu you are the reason why they are getting the concepts....
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Thank you sir for your explaination.. helped me on my exam....both TLC and ion exchange!
+bhagyajit das glad it helped you
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Superb explanation...👍
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Thanks alot sir 🙏very nice lecture
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In sha Allah sir
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Thank you sir🙏🙏.
You're welcome
Very beautifully explained
You also needed to explain that some plates won't show up in UV light so they have to be sprayed with whatever spray is needed. Also, the Rf value isn't for quantitative analysis. It's used to ID the sample against a standard.
Very clear and nice explanation
Also in some cases IODINE is used. So that the compounds absorb iodine , which will show up as Brown spots.
Also Ninhydrin solution for detecting Amino acids
Thanks a lot. It helps me a lot
You're welcome
Tq sir, very clear and easy way of understanding the concepts , The way of teaching u did was fantastic sir, thanks a lot.
Sir wonderful explanation sir..can you plz describe mushroom cultivation compose, materials used in composing and different formulation used in composing , compost preparation and its methods...it will be your most kindness
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Thank you very much sir.
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thanks for sharing such informative knowledge
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good job important video for freshers
Thank you
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Thanks sir. you really enhance my understanding on the whole chromatography chapter.
+archna that's nice to know
Your videos help me a lot!
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Easy understanding each and everything..
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We were just about to start using this principle in our lab. Good timing and great video.
+Nyan Rapier hope I can help
So the question is why does polar compounds interact with silicagel (also very polar) stronger than non-polar compounds?
This is because silica gel is also a polar substance. Hydrogen bonding is responsible for the polarity.
Very nice explanation sir thank you for making video on this topic 🙏
You're welcome. Glad to hear that you're getting benefit from my lectures
Beautifully explained
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Thank u sooo much sir...it will help for me
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Love your teaching!
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Thank you so much 😊 Sir 🙏
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Thanks for ur lectures l passed molecular florensics well last semester thnx ....am back thnx
Okay. Thank you
what is the effect of the similarity or difference polarity of the mobile phase and the solute we are computing; does it make a difference. i think i did not hear you saying about it except you said about the polarity of the stationary phase versus the solute.
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ThanQ sir...simple method say explain kiya Gaya hay..I know every one pick this lec
Excellent demonstration
sir ur teaching is awsm. can u plz make vedio how to do seminar on gel filtration topic plz
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Superb explanation. Tq very much
Awesome sir.
Thank you
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I like sir ur explanations. Please Try to give like this other topics.
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Sir Can you Make Video On Micro-Organism Because My Science Exam Is On 19th
Please Try To Make The Video Before My Exam It Will Help Me A Lot
3. Silica gel is often used as a Stationary Phase is TLC Chamber. What role does it play in the chamber? (2mks)
Thank you sir 👍😊
You're welcome
sir ...the class was good...nice lecture....please do more lyk this...
+NAVANEETH s Kumar thank you