thank u so much... I guess shomu's biology is the best channel for every type of query... very very helpful and the way u explain is understandable... nice job sir... :-)
Sir What if smaller molecules enter the void along with larger.. its not compulsory that smaller take the pores only, they may enter the voids too. Than first to be eluted is smaller particles in this case..
Thankio Sir I like Your Teaching method, Your video Satisfied me in my aim and many many thanks at last iwant to say you Sir please explain Uranic Acid Pathway If You can. Thankyou Sir
sir , when we will pour a sample it may contains undesirable materials but we get product according to size. So undesirable materials are also disributed in product according to size. Then how we can separate undesirable materials.
Another difference between gel electrophoresis and size exclusion chromatography is that smaller fragments will run faster and larger ones will run slowly in gel electrophoresis while in size exclusion chromatography smaller molecules will come slowly and larger ones come faster, am I right Sir?
If I'm using agarose instead of sephadex beads as the matrix of the column then how do the protein of high mol. wt. will elute faster than that of lower ones
thanks for your big healp . I just look to go deeper in technics.i mean how to do calculations of the V0 or Retention volume and time . how to read a cromatoramme if you can o that I'll be thankfull
hlw sir , u teach us very smartly . tx for that . but , somewhere i studied that some mixture of protein standards added first before mix of protein . can you explain why is it ?
HI! Sir I have this question above could you please help to me solution please. Proteins may be quantified by spectrophotometry at 280nm. Explain the underlying principles of this technique and outline the advantages of this assay over alternative methods.
column chromatography covers many types of chromatography like ion exchange, affinity and also gel filtration. so gel filtration is a type a column chromatography since its run through a column
If sample contain both smaller and larger prtn then how only larger prtns I'll come through pores why can't small prtns come through the same pores from which larger one comes
anthati mastan small particles has two route: through pores and from surrounding with large ones the letter has one so low percentage of small will run beside large
In another lecture... they said larger molecules will travel slower as they want to find alternative way, around the st phase and but small molecules can easily move through the pores in them and travels faster.... Actually which statement is true ??🤔🤔
Aravind SR I think the one where large mol. Are eluted first is much more gripping concept because as you see the surface are for the large molecule will be greater and the way they had to find will be small in comparison to the small molecule which are stuck in the labyrinth of pores.....like a ant which has average speed way much faster than a cat but the cat will still outrun them.
You have a talent for teaching others. Huge thumbs up and thank you
Glad to hear that you're getting benefit from my lectures
RD Sharma is describing concepts specially for physics students...like that u r there fr us(chem & bio students)..thank u sir
Maths students* not physics
Thank you sir your videos are helping me. . .recently i came to know there is a chanel shomu s biology. . Nd this is a gift for lifescinces students
+Kuldeep kaur Dhaliwal thank you. Hope to serve you more
thank u so much... I guess shomu's biology is the best channel for every type of query... very very helpful and the way u explain is understandable... nice job sir... :-)
Thanks sir your lecture is very helpful for me because i am doing msc biotechnology
Sir u are a Gem 💎 , and I give thanks to God for it and I even thank God for giving us shomu sir 🙏
You're welcome. Glad you like it
Thank you so much sir your lectures are helpful and pretty easy to understand. Love from Pakistan
+Syed sahil Shah glad to hear that
Syed sahil Shah you are commenting near all vedios of him! Stubborn fan! Nice..
thnx suman....getting addicted to watch ur videos..so much of knowledge..
Incredible way of teaching. 💖👍
Thank you so much for appreciating my efforts
Tq sir u are very helpful to me to understand easy about chromatography.... Tq ...
You're welcome
It's.a.big honour to me to taking your lecture
Thank you for your effort for explaining each and every point clearly.
All ur video's helping so much for me nd to my frnds aa big lyk to uu sir 🤝👍 Tysm 😊
I understood in the first go. This video is really helpful.. 😄😊
I love ur videos. You literally explain everything very well
Thank you very much for crystal clear explanation
Thank you so much for appreciating my efforts
Its very easy to understand... Thank you brother ☺
You're welcome. Glad to hear that you're getting benefit from my lectures
Very very educative . thank u so much mr.Suman. LOVE U always
Nice
Tq very much dir....
my mdm teach very badly but u r soo good sir
Thank you. Glad you liked my lectures
Sir What if smaller molecules enter the void along with larger.. its not compulsory that smaller take the pores only, they may enter the voids too. Than first to be eluted is smaller particles in this case..
Thank u sir ur explanation is awesome ......
Thank you
Somu sir plz increase u r vedio volume u r explanation is good
super sir really you are helping me a lot.good explanation.thank u
very nice video for CC, keep going...
You're welcome
I have a query, so, does folded protein comes out first or unfolded according to their hydrodynamic volume?
really very helpful thing sir...thank u sir...provide some more videos on spectroscopy topics
these videos helped me a lot. thank you
thanks shomu da...you helped me alot. I want to meet you personally
Nice explaination
Thank you
Thankio Sir I like Your Teaching method, Your video Satisfied me in my aim and many many thanks at last iwant to say you Sir please explain Uranic Acid Pathway If You can. Thankyou Sir
sir , when we will pour a sample it may contains undesirable materials but we get product according to size. So undesirable materials are also disributed in product according to size. Then how we can separate undesirable materials.
Another difference between gel electrophoresis and size exclusion chromatography is that smaller fragments will run faster and larger ones will run slowly in gel electrophoresis while in size exclusion chromatography smaller molecules will come slowly and larger ones come faster, am I right Sir?
Thank you so much sir ❤️🥰
You're welcome
i want to lecture and assignment on protein purification in detail .
I really like your teaching style that,s so good .so, i want to take more lecture
Hi... thanks for your all lectures..... would you please upload about various types of detector used in chromatography..... thanks again
If I'm using agarose instead of sephadex beads as the matrix of the column then how do the protein of high mol. wt. will elute faster than that of lower ones
thanks for your big healp . I just look to go deeper in technics.i mean how to do calculations of the V0 or Retention volume and time . how to read a cromatoramme if you can o that I'll be thankfull
hlw sir , u teach us very smartly . tx for that . but , somewhere i studied that some mixture of protein standards added first before mix of protein . can you explain why is it ?
We liked it a lot thanks
Hi SIr, I love your videos a lot...Can you please make a video about chromatofocusing
helped me alot thanks
+Akshay Zagade you're going welcome
HI! Sir I have this question above could you please help to me solution please.
Proteins may be quantified by spectrophotometry at 280nm. Explain the underlying principles of this technique and outline the advantages of this assay over alternative methods.
please sir..take 1 lecture.on this topic
what is flash chromatography?
Thanku sir it's very nice
What about Gel permeation chromatography ? Is it same ?🤔🤔
Yes
Plz explain affinity elution
Good sir 🙏✍️
You're welcome
Is it adsorption column chromatography?
How to separate sample and mobile phase from the reservoir
Please make a video of analytical method development in hplc in hindi
Thanks very amwasome bro
So, gel filtration chromatography is the same as column chromatography? I am little bit confused with terminology
column chromatography covers many types of chromatography like ion exchange, affinity and also gel filtration. so gel filtration is a type a column chromatography since its run through a column
sir plzzzzz upload all video for neet exam
Thank you so much.
You're welcome
Why take about application of.gel filtration chromotography?
If sample contain both smaller and larger prtn then how only larger prtns I'll come through pores why can't small prtns come through the same pores from which larger one comes
anthati mastan small particles has two route: through pores and from surrounding with large ones the letter has one so low percentage of small will run beside large
I though the reason is buckflow not just gravity
How can be the unit of hydrodynamic volume is angstrom??
understand easily
From this GPC data how can I find out the molecular weight of my synthetic compound
I think there is now a technologically advanced set-up where there is a detector such as UV-Vis and refractive index.
many Thanks
Thank you sir
Thanks a lot,
You're welcome
@@shomusbiologyofficial it's helping my homework from my Professor
Helpful
You're welcome
Well sir
Thank you
Sir make video on cell , chromosome in hindi please
Okay
it's good that u helped us but better you give no adds during lectures, so that one can completely concentrate. what are you talking about ?
thanks sir
thanks
sir elution means
Elution mean to carry
nice
Sir aap har aak subject pa lecture kase dete ha
I prepare myself and deliver
Niceeeee sir
You're welcome
In another lecture... they said larger molecules will travel slower as they want to find alternative way, around the st phase and but small molecules can easily move through the pores in them and travels faster....
Actually which statement is true ??🤔🤔
Aravind SR I think the one where large mol. Are eluted first is much more gripping concept because as you see the surface are for the large molecule will be greater and the way they had to find will be small in comparison to the small molecule which are stuck in the labyrinth of pores.....like a ant which has average speed way much faster than a cat but the cat will still outrun them.
🌷🌷
👍
🤔🤔
Thank you so much 😊 Sir 🙏
You're welcome
Thank you so much sir 🙏😊
You're welcome
nice
👍