Correction as sir mentioned in video ..polar solvents and polar components move fast but mam said correctly ... The sample in polar in nature move slow because polar components have strong interaction with the polar stationary phase ,causing them to move slowly through the TLC plate And Sample is non polar ..move fast This is because non polar components weaker interaction with the stationary phase in TLC, allowing them to move quickly through the TLC plate. The reduced affinity between the non polar sample and polar components results in faster migration on TLC plate.
If you use a polar solvent as the mobile phase in silica TLC, the polar compounds will travel faster, and the non-polar compounds will travel slower. This is because: - Polar solvents, such as methanol, acetonitrile, or water, can form hydrogen bonds with the silanol groups on the silica gel, reducing the interactions between the silica gel and polar compounds. - Polar compounds can dissolve and move more easily in the polar solvent, increasing their migration speed. - Non-polar compounds, on the other hand, have weaker interactions with the polar solvent and are more retained by the silica gel, slowing their migration. By using a polar solvent, you're essentially reversing the elution order, making it suitable for separating polar compounds. Some common polar solvents used in TLC include: - Methanol - Acetonitrile - Water - Ethanol - Acetone Keep in mind that the choice of solvent depends on the specific compounds you're trying to separate and their properties.
@@chemcatalyst.. I have a question, if solvent is intermediate ( in between polar and nonpolar) then movement of components on the basis of mobile phase or stationary phase?
Thank you so much ma'am. Apko main bohot phyle se follow kar raha huun . Apse padhke sab topic Cristal clear ho jata hyy. Aur theory padhne ke baad practical main zyada samj ata hyy . 😊
Apart from the great work that you been doing since past years, which i have been following you with appreciation of your work and knowledge, I would see you as the next Kiran M Shaw in your domain. Apart from the knowledge that you impart towards biotech which drives the community by engagement, I think product or service based collaborative research would be great too.
Mam kamal ka smjhaya.❤ Please PCR and its types aur baki sb common use hny wali techniques ki ak ak kr ky vedioes upload kr dian. hm studenta k liy yeh bht helpful hngi.❤
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Ma'am please ,never stop we are with you .
Thank you very much for great explanation.
You are so down to earth person .
Thanks a lot .
Thanks so much 👍🏼
Correction as sir mentioned in video ..polar solvents and polar components move fast but mam said correctly ...
The sample in polar in nature move slow because polar components have strong interaction with the polar stationary phase ,causing them to move slowly through the TLC plate
And
Sample is non polar ..move fast
This is because non polar components weaker interaction with the stationary phase in TLC, allowing them to move quickly through the TLC plate.
The reduced affinity between the non polar sample and polar components results in faster migration on TLC plate.
If you use a polar solvent as the mobile phase in silica TLC, the polar compounds will travel faster, and the non-polar compounds will travel slower. This is because:
- Polar solvents, such as methanol, acetonitrile, or water, can form hydrogen bonds with the silanol groups on the silica gel, reducing the interactions between the silica gel and polar compounds.
- Polar compounds can dissolve and move more easily in the polar solvent, increasing their migration speed.
- Non-polar compounds, on the other hand, have weaker interactions with the polar solvent and are more retained by the silica gel, slowing their migration.
By using a polar solvent, you're essentially reversing the elution order, making it suitable for separating polar compounds.
Some common polar solvents used in TLC include:
- Methanol
- Acetonitrile
- Water
- Ethanol
- Acetone
Keep in mind that the choice of solvent depends on the specific compounds you're trying to separate and their properties.
@@chemcatalyst.. thank u so much
@@chemcatalyst.. I have a question, if solvent is intermediate ( in between polar and nonpolar) then movement of components on the basis of mobile phase or stationary phase?
Ever best Video. I learned a lot
Thank you so much
Thanks a lot mam.......I don't have deep knowledge regarding biotechnology practicals......you are a life savier mam...
Thanks 😊
Really Appreciable, Thank you so much ma'am 💙
thank you . please share
50 Subscriber completed. Thank you everyone.
Oh damn 🎉🎉🎉 wow kitna acha samjhaya hai mam aapne😊😊
Thanks a lot :)
So finally its clear now ☺️
Thank you so much ma'am. Apko main bohot phyle se follow kar raha huun . Apse padhke sab topic Cristal clear ho jata hyy. Aur theory padhne ke baad practical main zyada samj ata hyy . 😊
Thank You 😊
Thank you so much ma'am 🙇♀️🙇♀️🙇♀️🙇♀️
Want more videos regarding techniques
Thank You Rashmi, will try to create more such videos 😊
Very much informative thankyou so much maam and sir 😊
Thank you mam...aur bhi aisi tara vid chahye mam
Sure, Thank You 😊
Thank so much mam its just amazing session
Thanks 😊
Ma'am you are doing a great job.... Hume kafi help rhegi apke is video se😊 we love you ma'am
Thanks a lot :)
Thank you so much Mam and Sir....Very helpful explanation....❤
Awesome session❤❤👍👍
Thank you 😁
Informative video .. thank you so much ❤
Glad to have u ma'am waiting for more such videos ❤️
Thank you 😊
Mam upload more vedio about chemistry and chemistry experiments
Very nice explanation mam 🙏🙏
Apart from the great work that you been doing since past years, which i have been following you with appreciation of your work and knowledge, I would see you as the next Kiran M Shaw in your domain. Apart from the knowledge that you impart towards biotech which drives the community by engagement, I think product or service based collaborative research would be great too.
This comment made my day 😊, thanks for such kind words. 🙏🏻
Thats a perfect explanation thanks mam suddenly it's was very useful for me
@@ZainabDalvi-q3d thank you. Please share with your batchmates
Mam..ithni aasan se samajh gaya mam..Tq..
Thanks for watching :)
Highly appreciated step, thank you mam
Thank You 😊
🤩🤩🙏bhut bhut Sukriya mam
Thanks for watching.
Mam kamal ka smjhaya.❤ Please PCR and its types aur baki sb common use hny wali techniques ki ak ak kr ky vedioes upload kr dian. hm studenta k liy yeh bht helpful hngi.❤
Thanks for appreciating, we will try to upload more such videos.
Amazing mam👏🏻👏🏻😍 plz bring such more videos.. thankyou soo much mam😊🙏
Thanks Kajal. Yes we will upload more such videos.
very nicely explained maam.
Waiting for more videos like this ma'am 🤩
More to come!
Thank you 😊
Unknown sample hai. To mobile phase decide kaise kiya
Thank you so much mam ❤
Thank you ma'am ❤ it's very helpful
Thanks ☺️
Wah Ma'am new avatar mein 🙌
haha thank u ankit. ye avatar kaisa hai :)
Mam Microbiology se bhi related lab work procedure n detail plz upload kre ......
Thank You, sure will try to create more such videos.
Really helpful
mam is k through ham lysozyme ko egg white sy separate kr skty hein?
Madam near ftnir pr vedio banaye
Thankyou so much Mam ✨🌸
Welcome!! Thanks for watching.
How could I join the.
Please let me know.
Thanks with best hope.
Superb
Thanku so much mam this is very helpful
Mam i completed MSC in pharmaceutical chemistry please suggest me job after this?
Very helpful
Thank You 😊
Thankyou sumach 😅
Please upload more vedio regarding chemistry
Sure, we will try to upload Chemistry videos as well
How to join this program/ rupture biotechnology
Jo polar component rahega wo solute front ke jyada paas yani kam distance travel karega n kyuki silica bhi polar nature ka hai ?
yes
Maam aisa he kuch technique certification course bhi kijiyee❤
Good suggestion, we will consider this idea.
@@BaaYo 🩷🤗
Thanku mam...
Welcome 😊
Jo sample ko dry krna h vo hot air oven m krna h ya phir open air m
Hot Air oven
Solvents konsa he
Mam plz share video on transcription nd translation
Please follow my new youtube Baayo live.I have created this channel for lifescience students only
Here stationary phase is polar, non polar phase.and Mobile phase is non polar?
Thank u maam,
Welcome :)
7:27 meto dry ke bina hi karta hu 30 second funk marta hu or ho jata he 😢
Love u maam
@@shilpagawade9575 love ❤️
Mam please guide me mam aapne class 12 th ke bad kon sa course ki hai 🥺
Mai ne abhi 12 th ki hai
Mujhe iss field me jana hai
Mam yeh aapka khud ka lab 🧪🧫 hain kya?
Have you started a new company?
No, this is Rapture Biotech lab.
Maim,I want to learn practical and i where to learn please suggest
Mera job nahi lag rha hea
सर का आवाज बहुत कम है, वॉल्यूम फूल करना पड़ रहा है, 👂 फोन लगाने के पश्चात् भी
Ma'am
Private research institute me job kaise receive kre
Mam aapki ki sound nhi aa rhi ahi
Please try again, audio is clear in the video
🎉
❤🎉
Mam jis lab me record krri h usi lab me mai work krta hu😊
yesss :)
Me to TLC without gloves karta hu 😅
Or mere sir to direct hi spots kar dete he without any drawing 😂
😂
You teach very well as like you beautiful
Mam is type ki or videos lao
Yes, we are going to upload more such videos.
Apne to only one time hi sample load kiya h
College hme 10 times load karne ko kaha tha
Sahi kay h mam
@@dhanashrijitkar6181 10 times kyo.? 10 different sample or one sample 10 times?
@@BaaYo one sample
No entiendo nada ..pero estás guapa 😘😘😘😘
What is mobile face
Mobile phase.. its liquid buffer solution