WHAT IS CRISPR? - GENE EDITING EXPLAINED!

This series of short presentations on gene editing is brought to you by Dr Adam West, College of Medical Veterinary and Life Sciences at the University of Glasgow, Scotland.
www.gla.ac.uk/people/adamwest
This presentation describes the type II CRISPR system, which is an adaptive immune system found in bacteria that has been modified for use in genome editing.
This is part of a series ( • Genome Editing Explained ) that also covers
Zinc finger nucleases (ZFNs)
TALE nucleases (TALENs)
Using CRISPR/Cas9 for genome editing
CRISPR specificity
Precision editing
Homology directed repair
Prime editing
This presentation is primarily aimed at university students, researchers, clinicians and journalists interested in fields related to molecular biology and genetics. It is for education purposes only.
Please leave a comment to let us know whether this was helpful to you and what you think we should cover next. We are a new channel so please give us a like and share our video on your social media if you think others should see it. We have a lot of content coming up, so please subscribe!
More accessible presentations for a wide audience can be found in the “Explained Simply” section of this channel in the near future.
Artwork is © Adam West with the exception of images and data taken from publications referenced in these slides. References to reviews and journal articles are denoted by circled numbers at the bottom right of the slides.
Links to these numbered articles are listed below. We recommend these as your best way to begin further reading on this subject.
Publication List
17. Mali P, Esvelt KM, Church GM.
Cas9 as a versatile tool for engineering biology.
Nat Methods. 2013 Oct;10(10):957-63.
doi.org/10.1038/nmeth.2649
19. Garside EL, MacMillan AM.
Cas9 in close-up.
Nat Biotechnol. 2014 Apr;32(4):338-40.
doi.org/10.1038/nbt.2872
20. Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O.
Crystal structure of Cas9 in complex with guide RNA and target DNA.
Cell. 2014 Feb 27;156(5):935-49.
doi.org/10.1016/j.cell.2014.0...
Music credits
“Go, Icarus! Go!” by The Whole Other
“New Year” by Bad Snacks
(UA-cam Audio Library)

[@praneetasrivastava1919]

thank you so much for the genome editing series.

[@GenomicsGurus]

You're welcome. Glad it was useful

[@Joseph_C4JB]

Absolute legend, massive thanks from a 3rd year struggling on with his Genetics dissertation with only 1.5 years of in person teaching (strikes + a pandemic are a mean combo).

[@GenomicsGurus]

You’re welcome Joseph. Good luck with your studies!

[@fabianperson]

Hi! Thanks a lot for the great lecture series about genome editing! It helps a lot to understand the topic!
At 2:38 , do you mean double stranded RNA sequence instead of DNA?
Greetings from Berlin!

[@GenomicsGurus]

Thanks Fabian. Well spotted. Yes, dsRNA of course. Best wishes for your studies!

[@christiannissen2381]

Thanks for sharing your knowledge!
What does PAM stand for?

[@GenomicsGurus]

Glad you found it helpful Christian! Protospacer Adjacent Motif - the protospacer is the first ~20 bases that match the genomic target. The PAM is the next 3 bases in the genomic DNA that follow after the protospacer target, which Cas9 locks on to.