Can you give specifics on the prime editing? The first protospacer has no pam site so therefore will not nick it? The genomic spacer has the Pam therefore cas9 nicks it? The secondary guide rna knows to nick the first strand due to a Pam? Does the protospacer unbind after this and allow for the secondary guide rna? Are these all regulated by promoters?
@@GenomicsGurus You have utilised most of the time explaining already known things. Almost every researcher know these things. In my personal opinion, if you could use more time on prime editing, that could be more better, because it is new to many people. TALENS and ZFNs are now old stories..
Noor Bahadar Thanks for your feedback. This lecture is primarily intended for those who are relatively new to genome editing or those who only know a part of the story. Prime editing is explained at the end and there is a separate shorter video on that. Researcher focussed videos are coming up shortly, and prime editing will be one of the topics, which will have critical analysis.
@@noorpk At the beginning of the video he clearly says that the lecture is intended for University students. I'm a uni student and he explains very well. I'd rate his lecture 5/5
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Thank you for your work. Excellent video!!! It is unfair you have not so many views. It definitely the best video about gene editing. Thanks!
Great :)
Very good lecture Dr Adam West, very in depth!
Thanks Jamie. I'll be quizzing you on what you have learned...
Thank you so much. You have explained it in a simple and understanding way. I rate 5/5.
Videos are really helpful. Thanks, we really appreciate your efforts.
You are very welcome. There’s more videos and tutorials coming each week, so consider subscribing 👍🏻
better explanation than my prof. who only says useless nonsense during the lectures.
Thank you, I was looking for a lecture like this for a long time)
Really good video and super helpful with all the sources!
Glad it was helpful!
well explained!
Thanks!
Can you give specifics on the prime editing? The first protospacer has no pam site so therefore will not nick it? The genomic spacer has the Pam therefore cas9 nicks it? The secondary guide rna knows to nick the first strand due to a Pam? Does the protospacer unbind after this and allow for the secondary guide rna? Are these all regulated by promoters?
I will give you 4 stars out of 5
Thanks a lot Noor! 👍🏻 What would you suggest we improve to get that fifth star?
@@GenomicsGurus You have utilised most of the time explaining already known things. Almost every researcher know these things. In my personal opinion, if you could use more time on prime editing, that could be more better, because it is new to many people. TALENS and ZFNs are now old stories..
Noor Bahadar Thanks for your feedback. This lecture is primarily intended for those who are relatively new to genome editing or those who only know a part of the story. Prime editing is explained at the end and there is a separate shorter video on that. Researcher focussed videos are coming up shortly, and prime editing will be one of the topics, which will have critical analysis.
@@noorpk At the beginning of the video he clearly says that the lecture is intended for University students. I'm a uni student and he explains very well. I'd rate his lecture 5/5
youre the best, thank you so much !!
Glad you found it useful Lucie!