Flow cytometry : basic principles | What the use of flow cytometry ? | Cell sorting by FACS

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  • Опубліковано 24 лис 2024

КОМЕНТАРІ • 271

  • @TheLionheart94
    @TheLionheart94 2 роки тому +26

    Currently applying for an acting senior role in an immunology laboratory as a biochemist. I haven't studied flow cytometry for many years and it is a core area that the role looks after, therefore this video has been a brilliant starting point to try and get my head around things so thank you very much! :-)

  • @angie565656
    @angie565656 4 роки тому +38

    I am French and I understood everything, thank you ! I’m applying to a job and I have an interview tomorrow (I have never done cytometry before 😰)

  • @danaab9735
    @danaab9735 Рік тому +7

    That's an excellent and a very simplified and straight to the point clarification! Thanks a lot! I love your channel.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  Рік тому +2

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  • @astahl1938
    @astahl1938 2 роки тому +20

    This was an incredible video! Excellent animations and easy to understand descriptions. Thank you for your work!

  • @H_golds
    @H_golds 2 роки тому +7

    Thank you thank you thank you for this series. This would have taken me hours to understand using google. Your videos are so easy to follow and concise, explaining the basics of a complicated technique in simple terms, and your visuals/diagrams are fantastic. Cannot thank you enough, you might have just saved my grade.

  • @earthandmedicine3667
    @earthandmedicine3667 3 роки тому +8

    Thank you! This was very helpful for my pathology rotation as a 4th year medical student.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому +1

      Glad to know that it was useful….please share my channel link with your medico friends.

  • @zaphodflys
    @zaphodflys 17 днів тому +1

    Very clear explanation with great minimalistic diagrammatic explanation! Thank you!!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  16 днів тому

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  16 днів тому

      Glad you found it helpful! Please share this with your friends or classmates.

  • @josephdahdouh2725
    @josephdahdouh2725 Рік тому +2

    That was so clear thank you. I watched several on this topic, and could only now really understand where that graph comes from and how the pressure, flow, light, size, and complexity all somehow play a role in the final result of the diagram and the separate forward and side diagrams. Thanks, didn't expect I would understand it this well...

    • @animatedbiologywitharpan
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    • @josephdahdouh2725
      @josephdahdouh2725 Рік тому

      @@animatedbiologywitharpan I wish I could, but I come from a broke ass country, and I am almost broke... Me leaving a comment will give this video slightly more traction and will indirectly help this video get more views.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  Рік тому

      No problem, please share the link with your friends

  • @heleniroanya
    @heleniroanya 4 роки тому +7

    The way you explained this oh my gosh, I cannot thank you enough!

  • @ramenscreams231
    @ramenscreams231 2 роки тому +3

    thank you so much for this explanation! keep it up, your videos are so informative yet concise and easy to digest.

  • @phoebejuma6954
    @phoebejuma6954 5 місяців тому +2

    Wonderful. Makes it so much more clear!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  5 місяців тому

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @Gagaworshipper
    @Gagaworshipper 4 роки тому +6

    thank you! this was a very nice intro before going to class

  • @yasasiperera2948
    @yasasiperera2948 4 роки тому +1

    Hi I'm from Sri Lanka. Thank you for the very clear and comprehensive explanation.

  • @brianayee884
    @brianayee884 2 місяці тому +1

    Very much appreciated this video. Can't wait for more!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 місяці тому +1

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

    • @brianayee884
      @brianayee884 2 місяці тому +1

      @@animatedbiologywitharpan Will do!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 місяці тому

      @@brianayee884 Thanks a lot

  • @Harold_Ofori
    @Harold_Ofori 2 роки тому +1

    Very clear and straight forward about FC basics. Thank you

  • @commongrade89
    @commongrade89 2 роки тому +1

    Great explanation! And it only took mere minutes. You are a wonderful teacher👍

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Small contribution like $5 means a lot for us.

  • @rezkyilhamsaputra8472
    @rezkyilhamsaputra8472 Рік тому +1

    Thanks a lot, it really helped me understand the concept in just a few minutes

    • @animatedbiologywitharpan
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  • @roshnibhatt2801
    @roshnibhatt2801 5 місяців тому +1

    Your videos are so much beautiful and well order manner, which is very important for CSIR exam, thank you so much for making this nice videos. keep going

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  5 місяців тому

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

    • @roshnibhatt2801
      @roshnibhatt2801 5 місяців тому

      @@animatedbiologywitharpan yes of course

  • @bardacha100
    @bardacha100 3 роки тому +1

    Man, many many thks for deep work and clear presentation!

  • @antoniotommasopinto3462
    @antoniotommasopinto3462 Рік тому +1

    thanks so much, this is a very clear summary of flowcytometry

  • @samantadm8269
    @samantadm8269 11 місяців тому +2

    Thank you!! This helped me so much for my exams. Keep it up ❣️

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  11 місяців тому

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

    • @samantadm8269
      @samantadm8269 11 місяців тому

      @@animatedbiologywitharpan of course I will do it (: stay motivated please! Your effort is very much appreciated

  • @drio9589
    @drio9589 2 роки тому +1

    Thank you sir! This is a very good, clear, and concise video.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому +1

      Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance

  • @sunlydia7718
    @sunlydia7718 11 місяців тому

    So a nice video, very clear.It's a good beginning for my immunology lab

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  11 місяців тому

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @fatima8305
    @fatima8305 3 роки тому +3

    Thank you so much! Your effort is appreciated.

  • @truc1201
    @truc1201 Рік тому +1

    I started my day watching your video (I am going to a course of Cytometry today but it will be in French, which will be complicated for me). In short, you saved my life😂 Thank you so much

  • @candiceord1590
    @candiceord1590 7 місяців тому +1

    Great simple explanation, thank you!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  7 місяців тому

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  • @wbgw6476
    @wbgw6476 7 місяців тому +1

    This was a WONDERFUL video! Thank you very much!! :)

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  7 місяців тому

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @thunderstorminmyblood3705
    @thunderstorminmyblood3705 Рік тому +1

    super helpful and clearly explained, thank you!

    • @animatedbiologywitharpan
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  • @geraldineduignan8322
    @geraldineduignan8322 3 роки тому +1

    Very clear introduction. Thank you

  • @user-lg4zm5nj8t
    @user-lg4zm5nj8t 3 роки тому +3

    Well presented. Thank you.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому +1

      Please share my channel link with your friends and help me to reach big audience

  • @tripathyseema
    @tripathyseema 4 роки тому +2

    Thank you . It's very helpful and easy to understand the concept .

  • @meghasharma2505
    @meghasharma2505 Рік тому +1

    So wonderful explained! Thanku so much!

  • @simmywong3538
    @simmywong3538 2 роки тому +2

    Thank you so much for the incredible video!! :) May I know why PBMC instead of whole blood is used in flow cytometry?

  • @tinepone
    @tinepone 2 роки тому +1

    Phenomenal .. I understood so fast!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
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  • @mariatso3889
    @mariatso3889 3 роки тому +1

    thank you, this was so easy to understand
    keep up the good work!

  • @ceylan4948
    @ceylan4948 2 роки тому +1

    Damn nice video thank you! This might have saved my bachelor thesis!

  • @anyoon8829
    @anyoon8829 Рік тому +1

    The side detector seems to focus on how long the cell is. The forward detector can only detect how wide the cell is. Gravity factor comes into play on this machine

  • @gkgk8949
    @gkgk8949 4 роки тому +1

    Very clear explanation. Thank you

  • @natalias374
    @natalias374 2 роки тому +1

    thank you very helpful for chemical biology mid term

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

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  • @shubhanginisrivastava672
    @shubhanginisrivastava672 3 роки тому +5

    Great work!! you explained it very well and made it look easy👏👏
    Also i cant find ur next video about FACS, can you pls provide link of FACS video?

    • @tejaskhire
      @tejaskhire 2 роки тому

      I was also looking for FACS video. Can I get the link? Thanks !

  • @maria-br7ki
    @maria-br7ki 3 роки тому +3

    thank you so much for the explanation, this has been incredibly helpful! x

  • @dmoots2663
    @dmoots2663 Рік тому +1

    this was so so so helpful, thank you!

    • @animatedbiologywitharpan
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  • @EthanRiver
    @EthanRiver 3 роки тому +1

    It’s very clear, helps me a lot!!!👍

  • @maisashaikh4099
    @maisashaikh4099 7 днів тому +1

    Best explanation 👍👏👌

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  7 днів тому +1

      @@maisashaikh4099 Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

    • @maisashaikh4099
      @maisashaikh4099 3 дні тому +1

      ​@@animatedbiologywitharpanyes sure ❤👍. I'm sure it will help them a lot too just the way it helped me.

  • @ushanikaushalya3044
    @ushanikaushalya3044 Рік тому +1

    Thank you for your amazing explanation🥰

    • @animatedbiologywitharpan
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  • @BernardCouvreur
    @BernardCouvreur 2 роки тому +1

    very complete and clear !

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

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  • @nikitalokhande4063
    @nikitalokhande4063 3 роки тому +1

    Thank you. Good explanation & presentation

  • @abhyarthanabehera4802
    @abhyarthanabehera4802 2 роки тому +1

    Wow. Thanks again for giving me a better idea. 👍

  • @milica9136
    @milica9136 Рік тому +1

    Hello! Thank you for this information! Its easy understandable! I have a few questions. What are the odds of flow cytometry? How likely is that device, that is based on flow cytometry, counts cells wrong? For example, to count lymphocytes into monocytes? In what cases device might be wrong (I am asking only in context of flow cytometry)? Could you direct me to the sources where I can read more about these questions? Thank you very much! :)

  • @vuhkhyhg3334
    @vuhkhyhg3334 Рік тому

    Thank you this was very helpful im so grateful for your channel

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  Рік тому

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @Paramapabani
    @Paramapabani Рік тому +2

    Thank you so much Hare Kṛṣṇa 🙏😇

  • @mr.lonely.S.T.
    @mr.lonely.S.T. 2 роки тому +1

    Thank you so much!🙆‍♂️This is really helpful!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

  • @bartomiejrogalewicz6423
    @bartomiejrogalewicz6423 2 роки тому +1

    Thank you very much for uploading this :)

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому +1

      Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance

  • @tayyabmohammad3845
    @tayyabmohammad3845 2 роки тому +1

    Nicely done sir . Appreciated

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
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  • @kiaekim3466
    @kiaekim3466 2 роки тому +1

    Thank you very much! It is so helpful

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
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  • @davideschiliro
    @davideschiliro 2 роки тому +1

    Amazing, you are very good

  • @ogedara9846
    @ogedara9846 3 роки тому +1

    TRULY HELPFUL!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому

      Reach of my channel is really low...please share my channel link with your friends and help me to reach big audience

  • @manjildiganta9556
    @manjildiganta9556 4 роки тому +5

    how do you read graphs that have 4 quadrants, and then in the x axis you have FITC and in y axix you have APC. thanks

  • @tashichopel5235
    @tashichopel5235 4 місяці тому +1

    excellent video

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 місяці тому

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @rivasarkar
    @rivasarkar 6 місяців тому +1

    Thank you. Helped a lot

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  6 місяців тому

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @daisylockshire1953
    @daisylockshire1953 Рік тому +1

    Very helpful, thank you!

  • @arindampaul5256
    @arindampaul5256 3 роки тому +1

    Again Sir you won heart, Thank you very much 🙏

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому +1

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    • @arindampaul5256
      @arindampaul5256 3 роки тому

      @@animatedbiologywitharpan Sure Sir;
      Sir, Mahendra Sonawane Sir is in TIFR now?

  • @sabaali6638
    @sabaali6638 2 роки тому +1

    amazing explanation!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance

  • @Nature-Walk-With-Me
    @Nature-Walk-With-Me 3 роки тому +1

    Excellent video

  • @prachijadhao8431
    @prachijadhao8431 3 роки тому +2

    Very helpful 💯👍🏻

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому

      Please share my channel link with your friends and help me to reach big audience .

  • @norab6022
    @norab6022 3 роки тому +1

    Excellent straight-forward video! Thank you!

  • @ahmedsadique9916
    @ahmedsadique9916 2 роки тому +1

    Really good content

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

  • @paramitadas1756
    @paramitadas1756 4 роки тому +1

    thank you soo soo soo much bro ..you are really great ..grateful to you

  • @kaustubhrege969
    @kaustubhrege969 4 роки тому +1

    Thank u for great explanation

  • @friendsforever-hi8ij
    @friendsforever-hi8ij 3 місяці тому +1

    Very nice video

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 місяці тому

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @koespiana
    @koespiana 10 місяців тому +1

    thank you so much for such a good explaination!

  • @teyang2144
    @teyang2144 Рік тому +1

    Thanks for this!

  • @SofiaColledan
    @SofiaColledan Рік тому +1

    thank youuuu awesome and clear!!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  Рік тому

      In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

  • @amanuelbekuma8373
    @amanuelbekuma8373 Рік тому +1

    Good work!

  • @samiaaabdulwahid4875
    @samiaaabdulwahid4875 3 роки тому +1

    Thank you for your explained

  • @fallseason5163
    @fallseason5163 2 роки тому +1

    Perfect 👍🏻

  • @laurenwalker7165
    @laurenwalker7165 2 роки тому +1

    Great video

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому +1

      Please share my channel link with your friends and help me to reach big audience

  • @QueenBee-kh7bx
    @QueenBee-kh7bx 3 роки тому +1

    thank you so much! very nice video

  • @totalrandomness1718
    @totalrandomness1718 3 роки тому +1

    Wow just wow... 👏👏

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому +1

      Please share my channel link with your friends and help me to reach big audience......keep on watching ....your comments gives me so much of motivation to make more content

    • @totalrandomness1718
      @totalrandomness1718 3 роки тому

      @@animatedbiologywitharpan sure thing sir

  • @libra1807
    @libra1807 2 роки тому +1

    very useful. thanks 👌

  • @tripathyseema
    @tripathyseema 4 роки тому +2

    Thank you sir .

  • @aishwaryamhatre5032
    @aishwaryamhatre5032 2 роки тому +1

    Thank you so much sir 🙏❤️

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
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  • @st6589-p8kl
    @st6589-p8kl 7 місяців тому +1

    Thank you so much!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  7 місяців тому

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @jacquelinemarshall129
    @jacquelinemarshall129 3 роки тому

    Great stuff thank you.

  • @magicalcosmos
    @magicalcosmos 4 роки тому +1

    very nice ...keep it up

  • @gowthamir8562
    @gowthamir8562 2 роки тому +1

    Helpful

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance

  • @fazrinanirzaf
    @fazrinanirzaf 4 роки тому

    Very helpful ! Thanks sir

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 роки тому

      Please subscribe to my other channel ua-cam.com/play/PL_tBg3lwcZBmp2oqVEdjuDfmO-wzRJubc.html

  • @shailvishrivastava4403
    @shailvishrivastava4403 5 місяців тому +1

    Thanks a lot sir

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  5 місяців тому

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @skyminds9192
    @skyminds9192 2 роки тому +1

    simply super.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      Please share my channel link with your friends.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

  • @sportsgenre6
    @sportsgenre6 5 років тому +1

    Please take full classes in unacademy plus it will help us a lot. Please bhaia

  • @gamelaalrohaidy3779
    @gamelaalrohaidy3779 4 роки тому +1

    Thank you so much it's great

  • @stardreamix786
    @stardreamix786 Рік тому +1

    Thank you so so much :)

  • @gloriasolomon6165
    @gloriasolomon6165 3 роки тому +1

    Wowww thank you so much

  • @snehalshende-z4h
    @snehalshende-z4h Рік тому +1

    Hello sir..... by that graph we can understand which one lymphocytes and which one is monocyte or neutrophile????? Plz ans my question

  • @sharafisharafi4229
    @sharafisharafi4229 Рік тому +1

    Thank you 🙏

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  Рік тому

      In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

  • @Kirsten9Heuer
    @Kirsten9Heuer 4 роки тому

    Very well explained - super helpful, thank you!

  • @bedouraleneze5042
    @bedouraleneze5042 2 роки тому +1

    Thanks a lot 😭🫶🏻🤍🤍🤍

  • @fallseason5163
    @fallseason5163 2 роки тому +1

    Would you please help in analyzing data resulted from flow cytometry in some examples?!?!

  • @madhurimachaudhuri3282
    @madhurimachaudhuri3282 7 місяців тому +1

    Do you have lecture notes for this?

  • @roove1537
    @roove1537 3 роки тому +1

    Good video! :D