Very well done, great for both undergraduates and the more advanced. I found especially useful the way you distinguished between the 3 different systems, which is often not clear to those who are first learning about the system.
Great video, just a minor suggestion: I would give a short general overview (basically the summary that you give at the end of the video) of what the system does and the goal at the beginning of the video and then go in detail.
Thank you so much. This is so helpful. Great video and great explanation :) I can understand about CRISPR system better in just twenty minutes. Thank you
If a bacterium can cut off piece of viral dna in the first encounter, there is no chance of it (viral dna) integrating the bacterial dna. So why this complicated mechanism is needed ?
At 8:12 you refer to the lower strand as the coding strand. I believe that this strand is actually the template strand because the newly synthesized strand would have the same sequence as the top strand. That would make the top strand the coding strand and the bottom strand the template strand.
You are right the coding strand is actually the Non template strand as it contains the SAME nucleotides as that of mRNA while the template strand contains the COMPLEMENTARY nucleotides
@@LKVPAWAR_ycmou as you know there are two strands, template and non template. And because template strand has the complimentary sequences with that of mRNA (the complementary rule) that means the another DNA strand (non template strand)has the same sequence as that of the mRNA (except T in place of U) that's it is called as conding sequence as it has THE SAME CODES as that of mRNA.
At 9:15, cas6e/cas6f cut at the junction of ssRNA and dsRNA. The graph is not correct. It should stop at the end of hairpin loop. But this is minor detail. Many thanks for the videos. This is the only CRISPR video in English I can find on youtube that teaches type I, II, and III. Thanks!
Informative content, with simple and useful animation. Having produced hundreds of science videos and animations, some simple suggestions for the sound track. First, write out a clear concise script. Do a second edit on the script. When recording the script, speak each line clearly, leaving a long pause in between each comment. Avoid ummms and awes! With audio editing software it’s easy to adjust the length of the audio to match the animation. With the audio editing software, it’s easy to recognize and find, ummms and awe waveforms and replace audio distractions with silence.
I like this vdeo.but i have question. Diff viruses always attacking due to crispr bacteria add virus genome snnipts .Does it harm bacterial Dna bcoz it is put in spacer Dna and how long will bacteria keep it in spacer Dna bcoz new viruses attacks bacteria i think bacterial genome will no space if many viruses attck for long time.plz clarify.
a really great video. I just dont understand one thing. where did you know where to cut. And how did crispr knew where its needs to cut and replace the dna/rna or modifie the dna/rna. As far as i understand it crispr splits the rna/dna up but where did know what you need to pick.
This video explanation is very helpful especially for a beginner like me. Could you please also explain how CRISPR/Cas9 disappear from the genome of an organism? Thanks.
Thank you ,it is very useful video . I have Q if virus invade a bacterial cell ,it will insert genome and use bacterial cell to produce compunents to produce new phage and finally, bacterial cell will lysis . the question is how can bacterial cell save piece of viral genome when it will be degredate after viral invading .
Do you think so too? Do you think that if you create a tax that taxes expenses and income lightly, as your parents and younger brother say, your job will be less profitable than when you were on income tax? It seems that Mr. Greta Thunberg, who insists on environmental protection, met former President Trump, but even if a unified political nation can be formed with Q Anon, if it remains an income tax, I disagree. If we do new taxation and digital taxation such as income tax and expense taxation, everyone will be profitable, so I will agree. I think it is nonsense not only to grasp the income of one country but also to [if you need to see the income of the whole world, fill the gap between rich and poor with income redistribution] because productivity does not increase. 2020/1/31/5/3 Create a tax of love. It is thought that you can live in "Love Country" by creating [income tax] [expense tax] [reverse poll tax (revenue reverse tax / basic income)], which is called love tax. By switching to taxation according to tax liability instead of tax-bearing power, stable economic fluctuations and powerfulness that capture capital diversified development rather than capital-accumulated development such as [democratic efficiency economy] and [loving economy] Let's create an economy where economic development can be obtained. Until now, the [capitalist economy] has been an emergency economy that helps those who spend capital, those who work hard, and those who are not profitable. From now on, let's live a comfortable life by not spending capital, living hard, helping profitable people, creating a normal and permanent economy. Get a ticket from "patriotism" to "happiness". 2021/1/28 To the love that is handed down: Hiroko Yakushimaru www.uta-net.com/movie/1305/BAazkAioWGI/ Nowadays, it is clear that it is an economy for work because it shares living money, does not share work money, deducts necessary expenses, and progressively taxes income. Instead, let's create an economy for living by income tax, expense taxation, and reverse poll tax. It is necessary for the retired people to do so. 2020/1/31 If love is "to become one" and the "capitalist economy" that achieves capital-intensive development fails, then do not seek the [love-based economy] that achieves capital-diversified development next. I wonder? Instead of the tax-bearing power that starts with income tax, expense taxation, reverse head tax (revenue reverse tax, basic income), etc., which is called [love tax], we have created a tax system that is organized in tax-bearing responsibility, and stable economic fluctuations and strongness. I think it is necessary to create an economy that requires economic development. It will require the development of a commons called a commons. Unfortunately, it is necessary to seek the development of those without capital, not the development of those with capital. Will everyone believe in my [loving economy] ...? 2021/2/1 君もそう思う？経費や収入に薄く課税する税金を創ったら、親や弟の言う通り、仕事が所得税の時より、儲からなくなっちゃうと想う？環境保護を主張している、グレタ・トューンベリさんがトランプ元大統領と逢ったみたいなのだけども、もし、Qアノンとかで、政界統一国家が出来るとしても、所得税のままで在るのだとしたら反対をして、新しい課税、デジタル課税として収入税や経費課税を行うのだったら、みんなが儲かるようになるので、賛成しようと想います。一国の所得の把握だけではなく【全世界の所得を見る必要があるならば、貧富の格差を所得の再分配で埋める】のは、生産性が伸びないのでナンセンスだと想うから。 2020/1/31・5/3 愛の税金を創ろう。愛の税金と云われる、〔収入税〕〔経費課税〕〔逆人頭税（歳入逆税・ベーシックインカム）〕を創る事により、《愛の国》に住むことが出来ると想われる。担税力ではなく担税責任に応じた課税に切り替わる事により、〔民主効率主義経済〕や〔愛情主義経済〕と云う、資本集積型発展ではなく、資本分散型発展を捉える、安定した経済変動と力強い経済発展が手に入る経済を創ろう。今までの〔資本主義経済〕は、資本を掛ける人、一生懸命働く人、儲かって居ない人を助ける、緊急時の経済だった。これからは資本を掛けない、一生懸命生活をする、儲かった人を助ける、通常時・恒久時の経済を創って、余裕のある暮らしをしよう。「愛国」発「幸福」行き切符を、手に入れるのである。 2021/1/28 語り継ぐ愛に：薬師丸ひろ子 www.uta-net.com/movie/1305/BAazkAioWGI/ 現在は、生活のお金を分け合い、仕事のお金を分け合わない、必要経費を控除し、所得に累進で課税するので、仕事の為の経済で在る事は明らかです。そうではなく、収入税や経費課税・逆人頭税によって、生活の為の経済を創りましょう。そうすることが、経世済民には必要です。 2020/1/31 愛が“一つになる事”で在り又、資本集積型発展を遂げる、『資本主義経済』が駄目になるならば、次に資本分散型発展を遂げる〔愛情主義経済〕を求めてはいけないだろうか？〔愛の税金〕と呼ばれる、収入税・経費課税・逆人頭税（歳入逆税・ベーシックインカム）、等から始まる担税力ではなく、担税責任にまとまる税制を創りだし、安定した経済変動と力強い経済発展が求められる経済を創る必要があると想う。それは、共有体という共有地（コモンズ）の開拓が必要になるだろう。資本が在るものの発展ではなく、残念ながらも、資本無き者の発展を求めていく必要が在る。 皆は私の唱える〔愛情主義経済〕を信じてくれるだろうか・・・・・・・・？ 2021/2/1
Thanku mam to share this video Its good enough to understand the mechanism of crispr bt i hv a query regaring this as u told about crispr mechanism to bacteria protect from viral infection as immunity, bt there is also the lytic or lysogeny cycle where they protect from adverse conditions what about ds?
Thank you for a very helpful explanation. It's not an easy topic and I will need to watch the video again a few times for it all to sink in but, essentially, and for the first time, I feel I do understand.
The Cas9 protein has six domains, REC I, REC II, Bridge Helix, PAM Interacting, HNH and RuvC. Why did you say it has two? Is that because you are only concerned with the two (HAN and RuvC) for the purposes of this video? Or are those two the only ones used in the current state of CRISPR technology?
@@biomedicalandbiologicalsci4989 The nice reviews are nice but the not so nice reviews are also very necessary for growth and learning your self because you don't know it all. I love your video and the motivation to help people understand. I have learned by you. Thank you. But! It still remains that the mRNA transcripts isn't complementary to the coding strand( 5'-3'). It is always complementary to the 3'-5' strand ( anti sense/ template strand). And this should be viewed as nothing but a closed loop feedback. Nice reviews feel nice but the feeling aside it does nothing by way of promoting learning as ' not-so-nice' type of reviews do. Thanks for sharing your knowledge. I enjoyed your class very much.
Hay i have a realy important question where and what would one need to buy in a super market or plant to explore ones undersanding so can i buy any enzymes in store to explorer ones own health
Gosh I saw a really good video before that was so clear, she really pooped my understanding here. She explains really well lab procedures but she sucks explaining biomolecular concepts
Yes when this mortal puts on immortality And this corruptible puts on The incorruptible Then comes the end If you have not heard God made the heaven and Earth and everything in it
Whats the difference between the three types of interference described here? Do different species of bacteria use different types of interference or do these three all occur in the same cell simultaniously?
So simply explained with details... And also very simple and nice animation.... Great work... Keep explaining topics like this.... Your video helped me a lot... Thank you 😊😊👏👏
Very well explain I think 1st (this video) is essential to create base to understand CRISPR . Thank you for breakdown it and explain it in the simplest way 🙂
Hello thank you for the interesting vedio, i really enjoyed it, but I have one question, Im smolecular biology and genetics student and I have studied that the coding strand is the strand that is identical to mRNA " thymine instead of uracil" but in the vedio you said the the coding strand is the strand that was transcribed into mRNA , can you explain this to me ? Thank you
really good. much more detailed than most videos available on the same topic.thanks.
True
Exactly!!
Very well done, great for both undergraduates and the more advanced. I found especially useful the way you distinguished between the 3 different systems, which is often not clear to those who are first learning about the system.
finally someone who explains this topic very clear and also detailed enough :) thank you , it was really helpful !!
Great video, just a minor suggestion: I would give a short general overview (basically the summary that you give at the end of the video) of what the system does and the goal at the beginning of the video and then go in detail.
Thank you so much. This is so helpful. Great video and great explanation :) I can understand about CRISPR system better in just twenty minutes. Thank you
Thank you for your comment :)
I believe that at 10:43 you wanted to say Cas6 homolog (as written in diagram) instead of Cas9.
If a bacterium can cut off piece of viral dna in the first encounter, there is no chance of it (viral dna) integrating the bacterial dna. So why this complicated mechanism is needed ?
tysm this was what i was looking for for so long ;-;
GOAT CRISPR Video
Thank you!!! Excellent for undergrads having trouble with the primary sources; it really helped me. * thumbs up *
At 8:12 you refer to the lower strand as the coding strand. I believe that this strand is actually the template strand because the newly synthesized strand would have the same sequence as the top strand. That would make the top strand the coding strand and the bottom strand the template strand.
You are right the coding strand is actually the Non template strand as it contains the SAME nucleotides as that of mRNA while the template strand contains the COMPLEMENTARY nucleotides
yeah right
Exactly right.. bottom strand is template ..
@@mohinitalwar2888 I didn't got the Non template strand concept please explain what u said in didn't understand it please
@@LKVPAWAR_ycmou as you know there are two strands, template and non template. And because template strand has the complimentary sequences with that of mRNA (the complementary rule) that means the another DNA strand (non template strand)has the same sequence as that of the mRNA (except T in place of U) that's it is called as conding sequence as it has THE SAME CODES as that of mRNA.
Thanks you so much, you explained clearly for this topic.
Really nice presentation, much more detailed. Wait for your next presentations
At 9:15, cas6e/cas6f cut at the junction of ssRNA and dsRNA. The graph is not correct. It should stop at the end of hairpin loop. But this is minor detail. Many thanks for the videos. This is the only CRISPR video in English I can find on youtube that teaches type I, II, and III. Thanks!
Informative content, with simple and useful animation.
Having produced hundreds of science videos and animations, some simple suggestions for the sound track.
First, write out a clear concise script.
Do a second edit on the script.
When recording the script, speak each line clearly, leaving a long pause in between each comment.
Avoid ummms and awes!
With audio editing software it’s easy to adjust the length of the audio to match the animation.
With the audio editing software, it’s easy to recognize and find, ummms and awe waveforms and replace audio distractions with silence.
Thank you so much for this vedio I exactly understand how crispr work
I like this vdeo.but i have question. Diff viruses always attacking due to crispr bacteria add virus genome snnipts .Does it harm bacterial Dna bcoz it is put in spacer Dna and how long will bacteria keep it in spacer Dna bcoz new viruses attacks bacteria i think bacterial genome will no space if many viruses attck for long time.plz clarify.
This was great! Now I finally understand. Thank you!
you are welcome :)
at first i thought it was Ariana Grande's voice teaching CRISPR and am like "whut"
Do you have this crispr 2 video as an article???
a really great video. I just dont understand one thing. where did you know where to cut. And how did crispr knew where its needs to cut and replace the dna/rna or modifie the dna/rna. As far as i understand it crispr splits the rna/dna up but where did know what you need to pick.
Literally very informative mam
Hi! Thx for the video, helps a lot. Is there any place i could find it as pdf or ppt?
4:09,3 steps
1-spacer acquisition,4:21
2-crRNA processing,7:30
3-interference,10:55
Many thanks for the video, it is very clear. Well done.
This video explanation is very helpful especially for a beginner like me. Could you please also explain how CRISPR/Cas9 disappear from the genome of an organism? Thanks.
Thank you so much!! well explained :)
Thank you ,it is very useful video . I have Q if virus invade a bacterial cell ,it will insert genome and use bacterial cell to produce compunents to produce new phage and finally, bacterial cell will lysis . the question is how can bacterial cell save piece of viral genome when it will be degredate after viral invading .
Sometimes the bacteria resist the phage so it can take a part of its genome, and pass this genome to the next generations.
Thank you very much for the explaination
very good explanation am able to better understand the processes involved
seems like it would of been a nice video if the closed captioning was fixed
Do you think so too? Do you think that if you create a tax that taxes expenses and income lightly, as your parents and younger brother say, your job will be less profitable than when you were on income tax? It seems that Mr. Greta Thunberg, who insists on environmental protection, met former President Trump, but even if a unified political nation can be formed with Q Anon, if it remains an income tax, I disagree. If we do new taxation and digital taxation such as income tax and expense taxation, everyone will be profitable, so I will agree. I think it is nonsense not only to grasp the income of one country but also to [if you need to see the income of the whole world, fill the gap between rich and poor with income redistribution] because productivity does not increase.
2020/1/31/5/3
Create a tax of love. It is thought that you can live in "Love Country" by creating [income tax] [expense tax] [reverse poll tax (revenue reverse tax / basic income)], which is called love tax. By switching to taxation according to tax liability instead of tax-bearing power, stable economic fluctuations and powerfulness that capture capital diversified development rather than capital-accumulated development such as [democratic efficiency economy] and [loving economy] Let's create an economy where economic development can be obtained. Until now, the [capitalist economy] has been an emergency economy that helps those who spend capital, those who work hard, and those who are not profitable. From now on, let's live a comfortable life by not spending capital, living hard, helping profitable people, creating a normal and permanent economy. Get a ticket from "patriotism" to "happiness". 2021/1/28
To the love that is handed down: Hiroko Yakushimaru
www.uta-net.com/movie/1305/BAazkAioWGI/
Nowadays, it is clear that it is an economy for work because it shares living money, does not share work money, deducts necessary expenses, and progressively taxes income. Instead, let's create an economy for living by income tax, expense taxation, and reverse poll tax. It is necessary for the retired people to do so.
2020/1/31
If love is "to become one" and the "capitalist economy" that achieves capital-intensive development fails, then do not seek the [love-based economy] that achieves capital-diversified development next. I wonder? Instead of the tax-bearing power that starts with income tax, expense taxation, reverse head tax (revenue reverse tax, basic income), etc., which is called [love tax], we have created a tax system that is organized in tax-bearing responsibility, and stable economic fluctuations and strongness. I think it is necessary to create an economy that requires economic development. It will require the development of a commons called a commons. Unfortunately, it is necessary to seek the development of those without capital, not the development of those with capital.
Will everyone believe in my [loving economy] ...?
2021/2/1
君もそう思う？経費や収入に薄く課税する税金を創ったら、親や弟の言う通り、仕事が所得税の時より、儲からなくなっちゃうと想う？環境保護を主張している、グレタ・トューンベリさんがトランプ元大統領と逢ったみたいなのだけども、もし、Qアノンとかで、政界統一国家が出来るとしても、所得税のままで在るのだとしたら反対をして、新しい課税、デジタル課税として収入税や経費課税を行うのだったら、みんなが儲かるようになるので、賛成しようと想います。一国の所得の把握だけではなく【全世界の所得を見る必要があるならば、貧富の格差を所得の再分配で埋める】のは、生産性が伸びないのでナンセンスだと想うから。
2020/1/31・5/3
愛の税金を創ろう。愛の税金と云われる、〔収入税〕〔経費課税〕〔逆人頭税（歳入逆税・ベーシックインカム）〕を創る事により、《愛の国》に住むことが出来ると想われる。担税力ではなく担税責任に応じた課税に切り替わる事により、〔民主効率主義経済〕や〔愛情主義経済〕と云う、資本集積型発展ではなく、資本分散型発展を捉える、安定した経済変動と力強い経済発展が手に入る経済を創ろう。今までの〔資本主義経済〕は、資本を掛ける人、一生懸命働く人、儲かって居ない人を助ける、緊急時の経済だった。これからは資本を掛けない、一生懸命生活をする、儲かった人を助ける、通常時・恒久時の経済を創って、余裕のある暮らしをしよう。「愛国」発「幸福」行き切符を、手に入れるのである。 2021/1/28
語り継ぐ愛に：薬師丸ひろ子
www.uta-net.com/movie/1305/BAazkAioWGI/
現在は、生活のお金を分け合い、仕事のお金を分け合わない、必要経費を控除し、所得に累進で課税するので、仕事の為の経済で在る事は明らかです。そうではなく、収入税や経費課税・逆人頭税によって、生活の為の経済を創りましょう。そうすることが、経世済民には必要です。
2020/1/31
愛が“一つになる事”で在り又、資本集積型発展を遂げる、『資本主義経済』が駄目になるならば、次に資本分散型発展を遂げる〔愛情主義経済〕を求めてはいけないだろうか？〔愛の税金〕と呼ばれる、収入税・経費課税・逆人頭税（歳入逆税・ベーシックインカム）、等から始まる担税力ではなく、担税責任にまとまる税制を創りだし、安定した経済変動と力強い経済発展が求められる経済を創る必要があると想う。それは、共有体という共有地（コモンズ）の開拓が必要になるだろう。資本が在るものの発展ではなく、残念ながらも、資本無き者の発展を求めていく必要が在る。
皆は私の唱える〔愛情主義経済〕を信じてくれるだろうか・・・・・・・・？
2021/2/1
Very helpful video about CRISPR.... Thanks alot for guiding
Thank for sharing this video. It very greatful.
Thanku mam to share this video Its good enough to understand the mechanism of crispr bt i hv a query regaring this as u told about crispr mechanism to bacteria protect from viral infection as immunity, bt there is also the lytic or lysogeny cycle where they protect from adverse conditions what about ds?
Yes, it was very good to understand easily. Thanx.
YOU ARE AMAZING!
why bacteria need crispr if it can cut the dna of the phage in the first place
This is one of the best detailed video on CRISPR i have seen in UA-cam
This was fantastic, i can now apply the technique of crispr in my research
A great help...thanks
Thank you for a very helpful explanation. It's not an easy topic and I will need to watch the video again a few times for it all to sink in but, essentially, and for the first time, I feel I do understand.
Detail information in simple language with presentation
Very well explained 👌
WHERE TRACR RNA FORMED? HOW USE IN GENOME EDITING?
thanks alot for easy explanations could u pls send me its refrences
Thanks, it is a very good explanation
can anyone tell me, effects and characters of short and long PAM?
Thank you very much! It saved my lifeeeee! :D
The Cas9 protein has six domains, REC I, REC II, Bridge Helix, PAM Interacting, HNH and RuvC. Why did you say it has two?
Is that because you are only concerned with the two (HAN and RuvC) for the purposes of this video?
Or are those two the only ones used in the current state of CRISPR technology?
so nicely explained ! A big thanyou for making me understand the whole process in a easy way !
excellent and very useful video and explanation
Excellent presentation. Ideal as an educational resource, for explaining how the system works in bacteria. Very well done, indeed.
Thank you for the nice review ... stay tuned :)
Biomedical and Biological Sciences very good explanation
@@biomedicalandbiologicalsci4989
The nice reviews are nice but the not so nice reviews are also very necessary for growth and learning your self because you don't know it all.
I love your video and the motivation to help people understand. I have learned by you. Thank you. But! It still remains that the mRNA transcripts isn't complementary to the coding strand( 5'-3'). It is always complementary to the 3'-5' strand ( anti sense/ template strand). And this should be viewed as nothing but a closed loop feedback. Nice reviews feel nice but the feeling aside it does nothing by way of promoting learning as ' not-so-nice' type of reviews do.
Thanks for sharing your knowledge. I enjoyed your class very much.
I have to do a presentation about the CRISPR/Cas System and couldn't find any conclusive, highschooler-friendly texts. Thank you so much.
Hay i have a realy important question where and what would one need to buy in a super market or plant to explore ones undersanding so can i buy any enzymes in store to explorer ones own health
Very nice video
Gosh I saw a really good video before that was so clear, she really pooped my understanding here. She explains really well lab procedures but she sucks explaining biomolecular concepts
what is the difference between CRISPR and RESTRICTION ENZYME
👏👏👏GREAT JOB. pls keep uploading videos on ur channel. 🤗🤞
pls explain this in russian its hard to follow with all the stuttering
Yes when this mortal puts on immortality
And this corruptible puts on The incorruptible
Then comes the end
If you have not heard
God made the heaven and Earth and everything in it
Great Video! Have suggested the channel to my professor so he can recommend throughout the next years
Whats the difference between the three types of interference described here? Do different species of bacteria use different types of interference or do these three all occur in the same cell simultaniously?
Does it always be 5' to 3' that became the template DNA? I was taught that 3' to 5' is the one that will undergoing transcription.
Wow I’ve watched this couple times and u explained it very well. Thank you
Amazing video !! You win it !!!
could you provide the Sources for me?
thank y very much and I like your video presentations. have y made any videos jumping genes(transposons)?
Thank you for your comment, I will try to do it :)
Great video! Thanks for sharing
thank you. it was an awesome video.
Thank you so much for your very nice explanation it wa really very helpful ❤️
Thanks very useful
Very helpful, however PAM position is on the non target strand 3’ to the protospacer
Jhhhnn
You made it so well
Coding strand is not the one transcribed 8:16, it should be the template/non coding/antisense/transcribed
Excellent video. Thank you so much!
very detailed and easy to understand this complicated mess. thank you
Very helpful.Thank you
So simply explained with details... And also very simple and nice animation.... Great work... Keep explaining topics like this.... Your video helped me a lot... Thank you 😊😊👏👏
Very well explain
I think 1st (this video) is essential to create base to understand CRISPR .
Thank you for breakdown it and explain it in the simplest way 🙂
Why does the bacteria need the crisper system if it can neutralize phage with first infection
Why start 3' and end5'? And 5' end 3'?
Wow, very help basics! Thank you
Biotechnology without microbiology would be impossible.
very usefull...plz make more videos.
Hello thank you for the interesting vedio, i really enjoyed it, but I have one question, Im smolecular biology and genetics student and I have studied that the coding strand is the strand that is identical to mRNA " thymine instead of uracil" but in the vedio you said the the coding strand is the strand that was transcribed into mRNA , can you explain this to me ?
Thank you
Nice go ahead
Great video I understood really nicely and loved the accent, very understandable (perhaps you can make an updated video on the subject).
thank you it was very clear and easy
What is the difference between gRNA and tracrRNA?
thank u for teaching me what my teacher did not teach but wants to test T.T
I have the captions on and everytime cas is said it comes up as a different word, cats, caste, gas, class, caps,case, castor, lol everytime
thank you! very clear
So far the best video on CRISPR basics. Easy to understand in layman's langauge...
Please i have question . How to explain having diffrent size of deletion by using the same two
gRNA
thank you, this was very clear and easy to understand, it helped me out a lot!
Thanks so much, exactly what I have been looking for 🙏
Great .. really Great
Wonderful explanation!!!
well well. this was so clear ma'am. i understand it well