Man! You are one heck of a great teacher. If we could all shrink ourselves and see everything in our cells and shrink even smaller to see the mechanisms DNA uses, you would be the perfect tour guide because you explain things that well.
@@andrewdouch hey andrew i must say you are the best at explaining these complicated concepts. In fact I have a sac on wednesday based on this and manipulation techniques and you have helped me understand all these and i feel like i’m more confident because of you. Thank you so much
I have 20 years of research experience and have heard several CRISPR-related talks. But this video explains the concept thoroughly..helped me get in touch with my basics..Thanks
Sir, I was so lost in CRISPR and didn’t know where to start and where to go. This is hands down tue best video on the topic. You went sufficiently into depth while keeping everything clear and concise. I would really appreciate next a detailed video on Prime Editing similar to this one, and comparing the 2 strategies, and talking about the problems with CRISPR such as high indel ratio, etc. Keep up the good work!
As you said, your video really fills the gap between those simple videos for the "common people" and those, who are made from experts for experts. Thank you!
OMG this video, yes perfect, for my exam i needed to learn how this works and needed to learn a paper written by fellow classmates, i dont think they saw this video bc this explanation is a 1000x better more understandable! Thank you!
This is the most splendid video on CRISPRCas9 system I have ever encountered. You have solved every question I have got on this topic! Thank you so much!
This video is really helpful for my research project relating crispr/cas9 to cancer treatment in high school. Thank you for making this, covering everything in sufficient depth to allow me to 'digest' the terminology in potentially intimidating articles made for graduate students. Your video is a great foundation.
You really gave the intuition to the whole CRISPR -Cas9 thing and cured the questions that were bugging me before , now its clear to a very good extent. Very grateful for this
The presentation is a perfect marriage between hitting all the key points, conciseness, proper elaboration, giving all the necessary details. Excellent job!
wow, what a great video. i've been looking for it for a long time and now there's finally one. without whiteboards, bad drawings, superficial subject matter and confusing slides. i'm studying biology and never understood the mechanism when my professor explained it. i then neglected it while studying. now i failed an exam repeatedly and have to go through the topic again. and you explained it so incredibly well, with all the details one needs to know, but not toooo detailed. thank you so much for that. i feel well prepared and hope there will be a question about CRISPR/Cas9 :)
Thank you. Thank you Andrew for your detailed and brilliant exposition of what actually takes place during homologous repair of dsDNA and insertion of foreign DNA through Cas9 CRISPR. I spent several hours looking at the schematics in books and articles and never could quite grasp how the homologous repair let alone DNA insertion occurred exactly. You are a superb teacher and I love the Brit accent.
Outstanding explanation. Going to start working in the lab next week, exploring the possibilities of CRISPR Cas 9 gene editing in patients with heart disease. Thank you for the refresh !
Andrew- outstanding presentation. I had some molecular biology training, but rarely if ever received instruction in the methodical and organized manner you use here. Thanks very much for taking the time to do this.
OMG! What a full-package video of animation & theory both. Very nicely described, Never thought this topic will be a cakewalk for me. Thanks a lot for making this.
Good Evening. I am also a teacher but your smile and such innocent communication, wonderful for me because students never get boring in such topic like crispr.
You have no idea how much of a big help you provided with this amazing video. I felt so lost because I could not understand CRISPR and I found this video!! I had to say thank you for million times.
Watched 6 vids on this topic and this video just set everything crystal clear...I was interested in knowing crispr...but now i feel like i can explain crispr...thank u🎉❤
Wow! that was awesome! Took me about 90 Minutes to work and write through this video, but I can‘t imagine anyone is doing a better job explaining this than you! Thank you so much! Definitely a new follower here 😊
As somebody of the general public just eager to understand the world around him and within himelf, I am very grateful for this going-to-more-depth video. After perusing this video at least three times and consulting some school textbooks for some basics, I was able for follow and understand. What an amazing and ingenious nano-world! And what an admirable and joyful manner of conveying knowledge! Chapeau!😊
Many thanks for a fantastic exposition. As a teacher of A level biology and senior examiner I always kept abreast of new developments in molecular biology, then I retired just before the potential of CRISPR was appreciated. To my shame I have only now got round to getting my head around it and your video has been a great shortcut to understanding.
As a food engineering student, it was amazing to be able to understand and set this amazing topic on my mind better. Thank you so much for sharing your knowledge in such a helpful way!
This was incredibily helpful! I am writing a term paper on CRISPR-Cas9 and its use for curing genetic disease such as muscular dystrophy. I was reading many research papers but could not keep up with the terminology and the heap of knowledge in them. This video gave a great foundation and I feel far more confident in approaching research papers. The visuals and explanations were elegant and show just how intricate and incredible life is. Thank you!
This was so helpful - I'm a clinician working through a clinical genomics qualification and this was just the right level for me!! You are an excellent teacher, thank you so much.
Man! I love how you explained everything i have watched this video over and over again and again and still find something new about the CRISPER CAS9 , thank you for an amazing and detailed video I hope you can teach us the complete biotechnology by this way!!😅 Thank you!! Cheers
Thank you so much, the video was so helpfull! It is actually the most informative and detailed explanation I have found. Its amazing how u deeply understand all the process step by step without skipping any crucial information. Im so glad I found your channel. You're doing an amazing job
our friend is a microbiology mmmajor(??), we've just had an interest in it since we were a kid, and she sent us this video. We were a *little* nervous at first that it'd be a hair beyond our understanding, but it came across all clear!! This stuff is so neat, it's never gonna stop being the coolest that chemistry taught itself programming by exploiting physics and now life exists. It's all so deeply intricate - an incredible dance of interlocking elements and systems, and it just!!! exists!!!! naturally!!!!! Anyways, this video is incredibly informative and effective at bridging that gap. Even for someone where the terminology isn't familiar, the explanation gets the idea across clearly and in detail such that an understanding is developed whether or not the particular vernacular sticks. Very well done!!
Thank you for making this video! Now I have a much bigger understanding on how CRISPR works. You explain everything clearly and it was easy to follow along with what you were saying
I am a fourth year biology student and every single day I wanted to give up studying, mostly because I couldn't bear the pressure from my professors. Today, for the first time during your video I got so fascinated that I couldn't stop rewatching.....for the first time..... That you so much for giving me hope and inspiration again. btw, I have an exam in molecular biology in 2 days))) thank you again!
Thanks so much for such a great video, really informative and easy to follow. This video and your one on human insulin have been amazing resources for my school research project. Thanks again!
You are an amazing educator, i am a year 2 biomedical science student and i have come across only a handful of professors or teachers that are as good and actually want to educate like you. Thank you very much.
OMG Thank you so much!! for CRISPR Cas-9, even though I read the powerpoint from the class, I still did not fully understand since it is very complicated and difficult mechanism to understand. Your video solved this problem, and I loved how you explain these step by step and those little details very clearly!!. It was so helpful for understand cas9 and even details of this process. Thank you!!!:)))))
This was very much informative yet explained in simple language video about CRISPR basic. It was more than helpful for me. Please make and upload explaining advanced forms of CRISPR (like prime editing, etc). This will help to build research methodology. Thanks.
Thank you so much! This was thorough and super well explained. I am an undergrad in biology and this was at the perfect level for my understanding. The animations were also a great help in visualization.
That's true - the mutation will be near the cut site. But a scientist can usually design the sgRNA direct Cas9 to cut more or less anywhere (PAMs are quite common - they are NGG, in other words anywhere there are two Gs together will be a PAM.
I personally have no exams I'm doing on this topic, I'm just a 15 year old who got interested in the topic and wanted a deeper explanation than most youtube videos offered, and I'm really glad I came across this video because it's an amazing explanation, diagrams that really helped me understand what's going on, and your teaching is awesome. I really liked how you addressed potential questions and I hope one day i could be able to learn how to teach others as good as you do because I love teaching things I've learnt to my friends. Just a few questions, I remember hearing that some cells prefer non homology or homology directed repair when given the option for both, do you have any explanation why that may be? Could it be due to availability of more or less homologous pairs or such? Then I'm not sure if this was mentioned, but what process allows the nuclease domains to cut the DNA. Lastly, I keep on hearing gene drive in the case of genetic engineering of mosquitos, is gene drive essentially just meaning all other homologous strands of DNA contain only a certain allele of the gene, as such when the other allele is damaged/cut it replaces it with the desired version and the cell essentially eliminates the cut allele for the rest of it's life cycle. And why is gene drive just exclusive to insects, and doesn't occur in mammals? Is there some other mechanism in mammals that ensures the homologous DNA can have either version of the alleles. Thanks alot for the vid, I've subscribed and liked the video, hopefully helps in more people seeing it, and I'm looking forward to watching more of your videos.
its great that you have this interest, but if you're wanting real answers to your questions, I'd recommend you ask specific questions in your research. its quite hard to follow what you're asking, especially with unnecessarily long sentences.
Such a nice explanation....how much hardwork you have put in.....that's unbelievable such fastidious details....bravo for such a great hardwork....really appreciated
Very nice video for making it understable for the intermediate people who want to deep dive further into the subject. But one question i have is around Minute 14:00 i think the uncut DNA on the 3 End has CG before PAM and therefore complemantary GC on the 5 End. But after the cut the last sequence is now GG and not CG anymore. Is this a mistake ?
Thank you very much! Although I am a foreign English speaker, your explications were very easily understandable to me. (I am sorry for grammar mistakes) In school, I want to present a subject in genetics and CRISPR-Cas9 is quite amazing! Even though there is no explication in our biology book... Perhaps, if it´ll works, I will present CRISPR!! Thank you, finding some good explications on the internet is not easy but now I understand!!
Amazing video Andrew! I have an assignment to give a presentation on CRISPR to my class and this video cleared the entire concept and made me confident enough to present it! Thank you! Also it is so heartening to see you enjoying teaching it, you almost seem like a kid who is about to see his Christmas presents haha...
THANK YOU SO MUCH MAN YOU HAVE NO IDEA HOW CONFUSED I'VE BEEN FOR THE PAST FEW WEEKS ABOUT HOW THIS WORK, my exams are in 2 weeks and we didn't learn this properly in school < 3
Andrew! Thank you for posting this. I'm a fiction writer and songwriter and not a scientist by trade or training, so I'll admit that there was a reasonable amount of information that I'll have to rewatch several times before I'm remotely confident I understand it. However, what you did here, bridging the gap between the oversimplified videos out there and the overly-technical videos was amazing and a gift to us all. Now, I have a much better understanding of CRISPR-Cas9's mechanisms and a little more insight into the whole gene therapy process. As an added bonus, your enthusiasm is infectious and absolutely sells the whole video! I do have a question for you. If scientists wish to introduce a new genetic sequence into a person's DNA (what I assume gene therapy entails), how do they ensure that the engineered sequence is found and used by the cell's repair mechanisms--particularly, in Homology Directed Repair? Is it a matter of deploying a large enough concentration of those sections so that the odds are high? AND would be a typical vector or means of introducing that desired repair sequence into each cell? A viral vector?
Hi - you're welcome. I'm glad you found it helpful. You are right that by putting a large number of copies of the DNA one wants incorporated into the cell, increases the chance that the repair machinery will use one of those as a template for HDR. But it's not a sure thing by any means. In fact the success rate is quite low - but still much better than traditional methods. The DNA is usually put into the cell by microinjection or electroporation.
An another OMG! I understood the insertion of a gene too! But in this case I have a question. How the cell differentiate between the fragment of DNA inserted and the homologous chromosome? I is inserted many copies of the fragment of interest?
Glad you liked it! And yes you are right-there is only going to be one natural homologous copy in there (sometimes not even one), but many fragments with the passenger DNA and homolgy arms are inserted. So it's a matter of probability. There is a much higher chance that the cell's repair mechanism will use an inserted DNA fragment - but occasionally it might use the natural homologus DNA.
Man! You are one heck of a great teacher. If we could all shrink ourselves and see everything in our cells and shrink even smaller to see the mechanisms DNA uses, you would be the perfect tour guide because you explain things that well.
That's a very creative way to say 'thank you'. You're welcome! - thanks for the kind words. I'm glad you found it helpful.
I totally agree I am currently learning about CRISPR-Cas9 at Uni and you have been way better than my professors. Thank you.
haha that sounds like a fun job!
@@andrewdouch hey andrew i must say you are the best at explaining these complicated concepts. In fact I have a sac on wednesday based on this and manipulation techniques and you have helped me understand all these and i feel like i’m more confident because of you. Thank you so much
@@andrewdouchIs CRISPR-CAS9 used in covid vaccines?
I am speechless.
I think with a teacher like you, biology would have been on the top of my preferred classes.
I wish you all the best.
So nice of you
I have 20 years of research experience and have heard several CRISPR-related talks. But this video explains the concept thoroughly..helped me get in touch with my basics..Thanks
Sir, I was so lost in CRISPR and didn’t know where to start and where to go. This is hands down tue best video on the topic. You went sufficiently into depth while keeping everything clear and concise. I would really appreciate next a detailed video on Prime Editing similar to this one, and comparing the 2 strategies, and talking about the problems with CRISPR such as high indel ratio, etc. Keep up the good work!
Glad it was helpful!
As you said, your video really fills the gap between those simple videos for the "common people" and those, who are made from experts for experts. Thank you!
OMG this video, yes perfect, for my exam i needed to learn how this works and needed to learn a paper written by fellow classmates, i dont think they saw this video bc this explanation is a 1000x better more understandable! Thank you!
So glad you found it helpful to you.
This is the most splendid video on CRISPRCas9 system I have ever encountered. You have solved every question I have got on this topic! Thank you so much!
Glad you liked it! Cheers.
This video is really helpful for my research project relating crispr/cas9 to cancer treatment in high school. Thank you for making this, covering everything in sufficient depth to allow me to 'digest' the terminology in potentially intimidating articles made for graduate students. Your video is a great foundation.
You really gave the intuition to the whole CRISPR -Cas9 thing and cured the questions that were bugging me before , now its clear to a very good extent. Very grateful for this
this is the far away best video about crispr on youtube
Thanks-that's a real compliment.
The presentation is a perfect marriage between hitting all the key points, conciseness, proper elaboration, giving all the necessary details. Excellent job!
Thanks Peter - that's a nice review! :)
You're welcome, Andrew! Keep up the good work.@@andrewdouch
wow, what a great video. i've been looking for it for a long time and now there's finally one. without whiteboards, bad drawings, superficial subject matter and confusing slides. i'm studying biology and never understood the mechanism when my professor explained it. i then neglected it while studying. now i failed an exam repeatedly and have to go through the topic again. and you explained it so incredibly well, with all the details one needs to know, but not toooo detailed. thank you so much for that. i feel well prepared and hope there will be a question about CRISPR/Cas9 :)
You're welcome. I'm really happy to know it helped you! Good luck on the test.
Dr.Andrew, It is my first time to write a comment to express my gratitude for someone, really really thank you for this masterpiece !
you're welcome! I'm so glad you found it helpful :D
Thank you. Thank you Andrew for your detailed and brilliant exposition of what actually takes place during homologous repair of dsDNA and insertion of foreign DNA through Cas9
CRISPR. I spent several hours looking at the schematics in books and articles and never could quite grasp how the homologous repair let alone DNA insertion occurred exactly. You are a superb teacher and I love the Brit accent.
Thanks Jacques. i'm glad you found it helpful. (btw my accent is Australian 😉)
Thank you for that very understandable description of the CRISPR-Cas9 system. Your explanation and your graphics are super.
Glad you like them!
Outstanding explanation. Going to start working in the lab next week, exploring the possibilities of CRISPR Cas 9 gene editing in patients with heart disease. Thank you for the refresh !
You're welcome. Cheers
Andrew- outstanding presentation. I had some molecular biology training, but rarely if ever received instruction in the methodical and organized manner you use here. Thanks very much for taking the time to do this.
You're welcome :)
I cannot stress enough how well you have explained this subject! Thank you very much!! Hats off to you, sir
Thank you for taking the time to leave such a kind comment. I'm so glad you found the video helpful 😀
I passed my one of my honours tests because of you. Thank you so much. Great teacher!
That makes me happy to know! Glad I could help
This video actually saved me!
You are the only reason I'm making it through year 12 biology 🤣🤣
OMG! What a full-package video of animation & theory both. Very nicely described, Never thought this topic will be a cakewalk for me. Thanks a lot for making this.
you're welcome :D
امیدوارم همه مسیر زندگیتون به همین راحتی و همواری باشه که این مبحث رو توضیح دادید. سپاسگذارم استاد گرانقدر.
Good Evening. I am also a teacher but your smile and such innocent communication, wonderful for me because students never get boring in such topic like crispr.
So nice of you
You have no idea how much of a big help you provided with this amazing video. I felt so lost because I could not understand CRISPR and I found this video!! I had to say thank you for million times.
You're very welcome!
This video was so helpful in getting my head around the CRISPR-Cas9 tool, you're a great teacher!
Thank you. That's very kind.
Watched 6 vids on this topic and this video just set everything crystal clear...I was interested in knowing crispr...but now i feel like i can explain crispr...thank u🎉❤
You're welcome! Thank you for the lovely comment 😊
Wow! that was awesome! Took me about 90 Minutes to work and write through this video, but I can‘t imagine anyone is doing a better job explaining this than you! Thank you so much! Definitely a new follower here 😊
Wow, thanks! Anyone who can listen to my voice for 90 minutes deserves a medal ;)
As somebody of the general public just eager to understand the world around him and within himelf, I am very grateful for this going-to-more-depth video. After perusing this video at least three times and consulting some school textbooks for some basics, I was able for follow and understand. What an amazing and ingenious nano-world! And what an admirable and joyful manner of conveying knowledge! Chapeau!😊
Thanks for that. Glad you found it interesting:)
What an amazing biologist, your enthusiasm is very contagious
Many thanks for a fantastic exposition. As a teacher of A level biology and senior examiner I always kept abreast of new developments in molecular biology, then I retired just before the potential of CRISPR was appreciated. To my shame I have only now got round to getting my head around it and your video has been a great shortcut to understanding.
Awesome. Thanks for that. I'm glad it was helpful to you. Best of luck for your retirement!
As a food engineering student, it was amazing to be able to understand and set this amazing topic on my mind better. Thank you so much for sharing your knowledge in such a helpful way!
This was incredibily helpful! I am writing a term paper on CRISPR-Cas9 and its use for curing genetic disease such as muscular dystrophy. I was reading many research papers but could not keep up with the terminology and the heap of knowledge in them. This video gave a great foundation and I feel far more confident in approaching research papers. The visuals and explanations were elegant and show just how intricate and incredible life is. Thank you!
Glad it helped you out :D
This was so helpful - I'm a clinician working through a clinical genomics qualification and this was just the right level for me!! You are an excellent teacher, thank you so much.
I'm so glad! - thanks for your comment it made me smile.
Man! I love how you explained everything i have watched this video over and over again and again and still find something new about the CRISPER CAS9 , thank you for an amazing and detailed video
I hope you can teach us the complete biotechnology by this way!!😅
Thank you!! Cheers
Thank you so much, the video was so helpfull! It is actually the most informative and detailed explanation I have found. Its amazing how u deeply understand all the process step by step without skipping any crucial information. Im so glad I found your channel. You're doing an amazing job
Glad it was helpful!
One of the best, explanation for CRISPR, hats off 🥰❤️
I have never seen an amazing explanation like this, u are a phenomenon
thanks for that :D
OMG! I Finnaly understood not only CRISPR, but also homologous junction repair! Thank you very much!
You're really welcome :D I'm glad you understood!
our friend is a microbiology mmmajor(??), we've just had an interest in it since we were a kid, and she sent us this video. We were a *little* nervous at first that it'd be a hair beyond our understanding, but it came across all clear!! This stuff is so neat, it's never gonna stop being the coolest that chemistry taught itself programming by exploiting physics and now life exists. It's all so deeply intricate - an incredible dance of interlocking elements and systems, and it just!!! exists!!!! naturally!!!!!
Anyways, this video is incredibly informative and effective at bridging that gap. Even for someone where the terminology isn't familiar, the explanation gets the idea across clearly and in detail such that an understanding is developed whether or not the particular vernacular sticks. Very well done!!
This is really an amazing video! Thank you so much for taking your time carefully preparing and making this video!!
That was a superb masterclass on CRISPR Cas-9, thank you so much for that.
You're welcome. Thanks for the kind words
Thank you for making this video! Now I have a much bigger understanding on how CRISPR works. You explain everything clearly and it was easy to follow along with what you were saying
Great to hear!
Wow! The greatest video ever! In every regard, it was the best. Very deep and very understandable. Thank you so very much. Please teach us more!
I am a fourth year biology student and every single day I wanted to give up studying, mostly because I couldn't bear the pressure from my professors. Today, for the first time during your video I got so fascinated that I couldn't stop rewatching.....for the first time..... That you so much for giving me hope and inspiration again. btw, I have an exam in molecular biology in 2 days))) thank you again!
I'm so glad to hear that-thank you for sharing :) All the best with your exam!
Thanks so much for such a great video, really informative and easy to follow. This video and your one on human insulin have been amazing resources for my school research project. Thanks again!
You are an amazing educator, i am a year 2 biomedical science student and i have come across only a handful of professors or teachers that are as good and actually want to educate like you.
Thank you very much.
Thanks for that. It means a lot :)
For the first time understood the topic from the scratch to more than expected to know. Wonderful explanation
I'm so glad it helped you out
i found a the best explanation for CRISPR , it's well explained sir thank you , I'm looking forward for more videos like this
I have read many articles and protocols and this is the only resource I have come across so far that explained in great detail! Thank you!
You're welcome :D I'm glad it helped
Amazing video! It is hard to find such specific information and detailed animations and explanations of these concepts. Invaluable! Subscribed.
Glad it was helpful!
Excellent video - well presented. Easy to understand.
OMG Thank you so much!! for CRISPR Cas-9, even though I read the powerpoint from the class, I still did not fully understand since it is very complicated and difficult mechanism to understand. Your video solved this problem, and I loved how you explain these step by step and those little details very clearly!!. It was so helpful for understand cas9 and even details of this process. Thank you!!!:)))))
You're very welcome!
I am glad and thankful that this video exists.
thank you! I'm glad you liked it
This was very much informative yet explained in simple language video about CRISPR basic. It was more than helpful for me. Please make and upload explaining advanced forms of CRISPR (like prime editing, etc). This will help to build research methodology. Thanks.
This was amazing and you are an amazing teacher! I am a new FAN!
HEY !!! YOU REALLY HELPED ME A LOT !!!!
A TONS OF APPRECIATES FROM ME !!!😂
THANKS A LOT PROF !!!!
New videos are always exciting to see!
best CRISPR education by far！
Thank you so much! This was thorough and super well explained. I am an undergrad in biology and this was at the perfect level for my understanding. The animations were also a great help in visualization.
Glad it was helpful!
the video served it purpose, thanks a lot mr andrew .
thank you so much, I was really struggling to visualise CRISPR and this has definitely made it easier for me!
Glad it helped!
That's true - the mutation will be near the cut site. But a scientist can usually design the sgRNA direct Cas9 to cut more or less anywhere (PAMs are quite common - they are NGG, in other words anywhere there are two Gs together will be a PAM.
Thanks a lot! This video really helps me know better about CRISPR, and your teaching is fabulous!
You're very welcome!
Amazing and in-depth, made it super simple and easy to understand, love you douchy
Glad it helped!
I personally have no exams I'm doing on this topic, I'm just a 15 year old who got interested in the topic and wanted a deeper explanation than most youtube videos offered, and I'm really glad I came across this video because it's an amazing explanation, diagrams that really helped me understand what's going on, and your teaching is awesome. I really liked how you addressed potential questions and I hope one day i could be able to learn how to teach others as good as you do because I love teaching things I've learnt to my friends. Just a few questions, I remember hearing that some cells prefer non homology or homology directed repair when given the option for both, do you have any explanation why that may be? Could it be due to availability of more or less homologous pairs or such? Then I'm not sure if this was mentioned, but what process allows the nuclease domains to cut the DNA. Lastly, I keep on hearing gene drive in the case of genetic engineering of mosquitos, is gene drive essentially just meaning all other homologous strands of DNA contain only a certain allele of the gene, as such when the other allele is damaged/cut it replaces it with the desired version and the cell essentially eliminates the cut allele for the rest of it's life cycle. And why is gene drive just exclusive to insects, and doesn't occur in mammals? Is there some other mechanism in mammals that ensures the homologous DNA can have either version of the alleles. Thanks alot for the vid, I've subscribed and liked the video, hopefully helps in more people seeing it, and I'm looking forward to watching more of your videos.
Thanks for the feedback - I'm glad you enjoyed it. Also glad you are doing biology for fun. That's the best way imho!
its great that you have this interest, but if you're wanting real answers to your questions, I'd recommend you ask specific questions in your research. its quite hard to follow what you're asking, especially with unnecessarily long sentences.
With this smiling face difficult things explained beautifully simply....I need such a good supervisor for PhD do you take PhD students
i finally understood how CRISPR-Cas9 works!!! thanks for making this video!
You're welcome!
1 video that contains everything I needed to know with the best animations ever, amazing wow. I would pay for this
you're welcome - glad you liked it. (those animations took me ages to make so I'm really glad you found them helpful)
This is awesome and professional. Thank you for explaining those concepts deeply and thoroughly
Such a nice explanation....how much hardwork you have put in.....that's unbelievable such fastidious details....bravo for such a great hardwork....really appreciated
It's my pleasure
I'm glad I found your video. You're a blessing to all students Crispr-Cas9 has caused headache to including me.
Glad to help
Very nice video for making it understable for the intermediate people who want to deep dive further into the subject.
But one question i have is around Minute 14:00 i think the uncut DNA on the 3 End has CG before PAM and therefore complemantary GC on the 5 End.
But after the cut the last sequence is now GG and not CG anymore. Is this a mistake ?
you literally saved my life! you explained this really detailed and simple at the same time. it was really really helpful. thank you sm!
You're very welcome!
Great teaching, Andrew. Thank you for helping me understand the CRISPR concept.
Amazing... I really appreciate your work. As a teacher, your videos were super-useful. Thanks
You're very welcome!
Thank you very much. Year 12 unit 3/4 bio student was extremely helpful and well articulated! Underrated channel will recommend to other classmates!
Cheers for that. 👍🏼
The best crispr-explanation video 🎉
Glad it was helpful!
Thank you very much! Although I am a foreign English speaker, your explications were very easily understandable to me. (I am sorry for grammar mistakes) In school, I want to present a subject in genetics and CRISPR-Cas9 is quite amazing! Even though there is no explication in our biology book... Perhaps, if it´ll works, I will present CRISPR!! Thank you, finding some good explications on the internet is not easy but now I understand!!
You're very welcome!
Thanks a lot Sir. I'm feeling more confident about my seminar presentation because of you.
That's great - I hope the presentation went well 😊
I'm retired military, retired state voc-rehab and now taking the cellular biology trip. Thanks for the ride!
You're welcome
Wonderful way to explain everything in a bit more than 30 minutes. You are amazing Andrew! And thank you so much, I needed this
Thanks for the feedback - I'm really glad you found it useful.
Superb video, excellent explanation and incredible pacing on the details!! Very much appreciated
Glad you liked it!
Crisp and clear explanation on crispr
One of my favourite instructional videos. Already visited this one thrice for refresher of the nuances! Hope to see more such content. Thanks Andrew x
You're welcome! That's very kind of you
Amazing video Andrew! I have an assignment to give a presentation on CRISPR to my class and this video cleared the entire concept and made me confident enough to present it! Thank you! Also it is so heartening to see you enjoying teaching it, you almost seem like a kid who is about to see his Christmas presents haha...
Glad it was helpful!
Thank you so much for such a beautiful explanation!
great video mate, made me understand everything so easy when my teachers in uni couldn't... thanks!!!!
Most excellent! Thank you. This is the best explanation of this topic I have had yet.
Literally such a good video. Thank you so so much!
Thank you sir, the best video about cas9 i've ever seen
Glad you liked it!
THANK YOU SO MUCH MAN YOU HAVE NO IDEA HOW CONFUSED I'VE BEEN FOR THE PAST FEW WEEKS ABOUT HOW THIS WORK, my exams are in 2 weeks and we didn't learn this properly in school < 3
Also, I do have a small question, won't the bacteriophage evolve over time to have chi sites making CRISPR-Cas9 ineffective?
@@fatemastersleepy4197 Great question. You’d think so, right?
Andrew! Thank you for posting this. I'm a fiction writer and songwriter and not a scientist by trade or training, so I'll admit that there was a reasonable amount of information that I'll have to rewatch several times before I'm remotely confident I understand it. However, what you did here, bridging the gap between the oversimplified videos out there and the overly-technical videos was amazing and a gift to us all. Now, I have a much better understanding of CRISPR-Cas9's mechanisms and a little more insight into the whole gene therapy process. As an added bonus, your enthusiasm is infectious and absolutely sells the whole video! I do have a question for you. If scientists wish to introduce a new genetic sequence into a person's DNA (what I assume gene therapy entails), how do they ensure that the engineered sequence is found and used by the cell's repair mechanisms--particularly, in Homology Directed Repair? Is it a matter of deploying a large enough concentration of those sections so that the odds are high? AND would be a typical vector or means of introducing that desired repair sequence into each cell? A viral vector?
Hi - you're welcome. I'm glad you found it helpful. You are right that by putting a large number of copies of the DNA one wants incorporated into the cell, increases the chance that the repair machinery will use one of those as a template for HDR. But it's not a sure thing by any means. In fact the success rate is quite low - but still much better than traditional methods. The DNA is usually put into the cell by microinjection or electroporation.
best video on CRISPR - PERIOD
Thanks Dom :)
An another OMG! I understood the insertion of a gene too! But in this case I have a question. How the cell differentiate between the fragment of DNA inserted and the homologous chromosome? I is inserted many copies of the fragment of interest?
Glad you liked it! And yes you are right-there is only going to be one natural homologous copy in there (sometimes not even one), but many fragments with the passenger DNA and homolgy arms are inserted. So it's a matter of probability. There is a much higher chance that the cell's repair mechanism will use an inserted DNA fragment - but occasionally it might use the natural homologus DNA.
This was brilliant‼️ especially the PAM part! 😂😂❤
I always appreciate a fellow Seinfeld fan. ;)
Incredible video, greatly increased my understanding, and your energy is contagious
Glad you enjoyed it!
Wow! What a presentation....best among all that I have watched.
Glad you liked it!
Excellent Andrew. I was thinking of doing something like this. But you have already done an exceptional job. 👍👍👍
Thanks! :)
Thank you this great explaination, best video I ´ve seen sofar in the internet about this topic !!! Thanks :)
Glad it was helpful!
Excellent video and explaination, thanks!
Glad you enjoyed it!
Thank youuuuuuuu. It's very useful. 🙏🏻🙏🏻🙏🏻