Enzyme Rate of Reaction Trypsin
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- Опубліковано 5 чер 2016
- AS/A level Biology required practical activity. Investigating the effects of a named variable (temperature) on the rate of an enzyme controlled reaction. Make sure you are familiar with the rate calculation and graph shown at the end of the clip.
This is a quick guide to a practical activity. Hopefully students have been taught to explain what is happening to the enzyme fully when answering exam questions. They must give detailed answers.
Excellent Ma'am. Really need it for my protein extraction project.
Science lesson from Gill Hudson, I’ve seen it all
Thank you very much for doing this :)
If you put the cross on the tube above the half way point it becomes visible in about 10 mins though the higher and Lower temperatures take around 30 mins. Make sure you use freshly bought Marvel. It doesn’t work if the milk is old and other brands are not good for this.
I was just wondering where you got the background information about what trypsin is? if you could provide a link or starting point for research, it would be appreciated :))
useful, clear video, thanks
Livid video man
HOw long does it take for the trypsin to hydrolyse the casein, as you did not state this?
thank you, it was helpful :)
great video thank you !
just wondering why it is better to use powdered milk?
its easier to make the concentration as you can change the mass of powdered milk and water according to your needs. If you need 1%, its gonna be 5g : 500cm3 or 1g : 100cm3
Hi, what is the reason for using pH7 buffer?
لقد ساعدني هذا كثيرا 💜
Apparatus
0.5% trypsin solution
3% solution of milk powder
pH 7 buffer solution
250cm3 beaker to use as a water bath
10 test tubes and 2 test-tube racks
Timer
Marker pen
Thermometer
1 10cm3 syringe
2 1cm3 syringes
Access to hot and cold water
Glass rod
Universal indicator paper and colour chart
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what stats test would this use?
Do you have an equipment list for this practical? :)
Ma 'am..i m teaching As level biology for the first time..and i hv difficulty in conducting practicals..i need help and guidance from an experienced person like you...how can i get resources videos and manuals to understand the practicals..
Anil Kumar where are you based and which exam board are you with?
Is it possible for you to share your results table please as I am unsure about how to draw it accurately?
Hi StephMy raw results in seconds for cross to become visible were 20oC - 239s, 30oC - 125s, 40oC - 69s, 50oC - 114s, 60oC - 313s, 70oC - 385s. The last part is a bit tricky. An easier way (and perfectly correct) to calculate rate of reaction is to use 1000/time for reaction to take place instead of 1/time taken for reaction to take place. This gives you easier numbers to plot particularly if your not great at dealing with numbers in standard form. Hope that helps. I will put a note on the video.
thanx aloot... n pls upload all the A's practical
I have uploaded them all. Everything except the A2 choice chambers prac which doesn't really need a video.
Why was the milk and enzyme left in the water bath before they were mixed together? Any reason why?
for the temperature to be the same
hi, Miss is the graph supposed to be a curve or a straight line to each point?
She’s not even your teacher what you on abut you cooking fool
@@hasnainn.m uhm why are you so mad about someone saying Miss
Thx
Why can’t we be sure 40°C is t optimum? Is it because it might be 35°C or 45°C?
Yes the optimum is somewhere between 30 and 50 degrees.
Hello. Can this experiment be done with commercial digestive enzymes?
Yes I should think so. Try it with any protease and see what happens.
@@biologypracticalsandrevisi8066 Thank you, I will try it.
Cute !
What’s the teachers name
Why did we use powered milk
I have no idea why but I tried many types of milk and Marvel was the only one that worked well.
how can we write a prediction for this experiment before even doing it ?
Jalal Chukwurah human digestive enzyme so it would be a good prediction that it would work close to human body temperature. .
@@biologypracticalsandrevisi8066 Thank you very much
Am I the only one who got pissed off by the camera man? Good video though
My name is pikachu respect me
No
@@Deepathy wow
Didn’t even help me with
Campuradux
I do not like the way in which, at 6:15 you state that the 'enzyme becomes denatured' it is in fact the active site of the enzyme that is denatured. Stating this in exam, even at GCSE level would score no marks.
The enzyme does denature. This changes the shape of the active site so no E-S complexes can form.
I reiterate, it is the ACTIVE SITE of the enzyme that denatures so that the enzyme substrate and enzyme active site no longer fit complimentary to each other, hence as you correctly state, no ES complexes form.
Damn didn't expect to see you here
blitzchika you agree with me, right Chika?
Umm I would say the enzyme as a whole and the active site are denatured. The entire enzyme loses its quaternary/tertiary structure as a result of broken bonds so seeing as the active site is part of the enzyme the bonds in it are broken/disrupted and its structure changes