This is an excellent lecture. I found this waaaaay more helpful than my teacher's explanation in my native language. I'm surprised of this great work :) Good job.
It's like magic!!! I mean in just 10 minutes I understand more than I would in a three hours lecture! ! If I ever take a degree it would be mostly because of you
Whenever someone try to teach they all talk about complex mechanics which is very hard to catch when studying the new concept but in your case it's so easy to understand the basic of the topic...
Omg u blew up my mind....english is not my first language but I understand bit by bit what u just taught.....very simple to understand...thanks a lot🙃🙃🙃
Some universities require to take biochem extended program , as USC, and u should pay 2000 dollars by ur own pocket and barely teaching u something literally their professor don’t know anything thank u Andrey u r here I love ur videos
Brilliant! I am not a native speaker but i really got it well. But i have a question. I wonder if someone can regain the proteins in a 100% purity or a near percentage purity by using this method. Thank you sir for these wonderful lectures.
You are the best. How about separation of polysaccharides? And I'd like to also emphasise that some molecules bear same color, in that case what will one do about timing?
So on a SEC machine, buffer is supposed to be constantly pumped through the system. What is the consequence if there is not a consistent flow of the buffer? This happened during a portion of our experiment and some overlapping peaks showed on the chromatogram.
Why doesn't the protein of interest dissolve back into the solution in the baggie, and do the other proteins that were in the baggie go through the semipermeable membrane? And if they do then why wouldn't the protein of interest also go through since salt concentration in the baggie is going down making it soluble in water?
Just a question, is there a factor that forces the small proteins to enter the pore? I mean isn't there also a chance that the small proteins won't enter the pore and just follow the same pathway that the larger proteins will take?
What is your day job? Also, have you obtained any degrees? Just curious as you seem to know about a lot of different aspects of certain science topics.
hi, thanks for the lecture it really helps.But i have a queston to ask, and I never do any protein purification work. So , I want to ask about the tsk gel filtration, how much volume does it require to run this method? And, can I get the molecular weight of the protein that I'm using using this method?
Basically the names are used interchangeably. However, to be more specific Gel Filtration Chromatography is one of the methods of Size Exclusion Chromatography. Size Exclusion Chromatography is an umbrella term. Other methods include Gel Permeation Chromatography where you use organic solvent as a mobile phase rather than aqueous solvent as in Gel Filtration Chromatography. Hope that helps!
I love you. You are the best instructor I have ever seen in my life, seriously. Thank you.
this is what I also say whenever I see his face when I'm finding lectures. lmao. I love this man. ALL RESPECT
This is an excellent lecture. I found this waaaaay more helpful than my teacher's explanation in my native language. I'm surprised of this great work :) Good job.
You’ve explained this much better than my professor that had studied in Harvard University . I really thank you
If I pass my final Enzymology exam tomorrow, i owe you everything.
It's like magic!!! I mean in just 10 minutes I understand more than I would in a three hours lecture! ! If I ever take a degree it would be mostly because of you
Thank you so much for getting me through biochemistry and anatomy. Have watched sooo many of your videos and they ALWAYS help!!!
Your lectures have made studying easier and concepts clear. Excellent lectures.
Your analogies are EXCELLENT! Thank you! You may possibly be the best teacher EVER.
Whenever someone try to teach they all talk about complex mechanics which is very hard to catch when studying the new concept but in your case it's so easy to understand the basic of the topic...
Beatle and ant example u have given in this lecture can explain this chromatography in a best way👏👏👏
the best teacher seriously. you explain it so well sir. that helped me a lot. thankyou
thank you so much For great explanation especially as Egyptian my English is not good enough so when you speak clearly and slowly it really helps
Really didn't use you much for organic chemistry, but using you right now for biochemistry. Your videos are amazing sir.
Omg u blew up my mind....english is not my first language but I understand bit by bit what u just taught.....very simple to understand...thanks a lot🙃🙃🙃
I have a chromatography report to write and this was so helpful thank you so much :>
zazooq88 awesome! you're welcome :)
I really think this Ak lectures tutorials are like gems to all student 🖤
Thank you so much, very clear short explanation 👍🏻
Thank you very much Sir...You are undoubtedly one of the finest teachers I have ever learned from...
i love ur way of presenting lectures..
thank you v much for all the help..
I did not understand the lesson in the classroom , but here *-* the way that you explain is very usefull , thank's a lot
Aminehordy glad to hear that mate :)
Basically watching this 1hr to laboratory quiz 🤗🤗🤗 how I feel about your lecture right now
your diagrams are always so clean and detailed damn i wish i could draw that nicely
getting my BSc degree thanks to this guy.
very clear explanations - thank you so much for posting this lecture!
Good Explanation Sir I Loved It and Every Video you post They are just So Simple to understand
i can not say thank you enough for your hard working wonderful explanations.
surely a best explanation.... thank you sir!!☺️☺️
Thank you so much sir your videos are really helpful. Also you explain way better than my professor 😍
I love the way you present your lecture wish you all the best
awesome work!! great explanation right from the basics!!
Thank you so much. I always enjoy your explanation. God bless you
Thank you very much.
Your explanations are very helpful
Wonderful,,,,, great,,, perfect,,, fantastic lecture,,,, excellent
You are a Real Game Changer 💕 dear 💝🤗
Some universities require to take biochem extended program , as USC, and u should pay 2000 dollars by ur own pocket and barely teaching u something literally their professor don’t know anything thank u Andrey u r here I love ur videos
thank you!
You are such a champion. Total BOSS.
You are a great teacher !!! keep on going !!
Thank you Boss for making me to understand this
Thank YOUUUUUU SOOO MUCH for all you DO.
Very clear explanations , thank you soo much 🧬
AWESOME video. Thank you so much.
Wonderful explanation!!!!!! keep it up!!!!!
Best explanation!
Brilliant! I am not a native speaker but i really got it well. But i have a question. I wonder if someone can regain the proteins in a 100% purity or a near percentage purity by using this method. Thank you sir for these wonderful lectures.
Thank You so much!!! these videos are so helpful!
you are seriously the best!
Best teacher🙌🙌🙌
Amazing explanation.. Thank you
Thank you so much for this! Watching this video truly helped me fortify a few ideas for my lab report :)
Thank you very much. This was so clear.
This is so helpful ! Thank you .
best lecturer everrrr
You are the best. How about separation of polysaccharides? And I'd like to also emphasise that some molecules bear same color, in that case what will one do about timing?
If the proteins under study were not
coloured, what alternative method could be used to monitor the fractionation process?
awesome explanation sir...helped alot
Excellent tutorial..thankyou!!!!!
Soon I will be able to give back. Thank you so much!
So on a SEC machine, buffer is supposed to be constantly pumped through the system. What is the consequence if there is not a consistent flow of the buffer? This happened during a portion of our experiment and some overlapping peaks showed on the chromatogram.
informative and easy explanation. Thanks!
you're welcome! :)
Why doesn't the protein of interest dissolve back into the solution in the baggie, and do the other proteins that were in the baggie go through the semipermeable membrane? And if they do then why wouldn't the protein of interest also go through since salt concentration in the baggie is going down making it soluble in water?
amazing video , thank you so muchh
Great video, keep it up! :))
best lecture
Best teacher
Thank you so much!
Thank you.
that was so helpful, thank you!
This is really good.
best explanation eeeeeveeer thank you
THANKYOU!! very understandable!
this is gold
Just a question, is there a factor that forces the small proteins to enter the pore? I mean isn't there also a chance that the small proteins won't enter the pore and just follow the same pathway that the larger proteins will take?
You are great!
One question I want to ask --where the mobile phase is...we have the stationary phase,mixture to be separated but mobile phase?
thank you vary vary much for that
What is your day job? Also, have you obtained any degrees? Just curious as you seem to know about a lot of different aspects of certain science topics.
Well explained
thank you very much
thank you sir
very helpful :) thank you .
Very good but why do you pour water on top ? is it simply to push the protein down?
Quirijn Zwier i think this is the reason why he pour water, but i can be wrong
thank you ,,, you are the best :)
great stuff, thank you!
J Williams you're welcome!
Thank you Teacher :D !
Couldn't possibly be clearer
hi, thanks for the lecture it really helps.But i have a queston to ask, and I never do any protein purification work. So , I want to ask about the tsk gel filtration, how much volume does it require to run this method? And, can I get the molecular weight of the protein that I'm using using this method?
excellent
excellent !!!!!
good jobs
Do you have any videos about ubiquination of proteins?
why is there a variation of volume when the protein emerge ??
thank u sir
wait is this related to column chromatography?
What can you do with the collected fractions?
Can you help me..I need some questions and their answer about this subject (chromatography ...electrophoresis)
can i use silica gel
What is the difference between Gel Filtration Chromatography and Size Exclusion Chromatography?
Basically the names are used interchangeably. However, to be more specific Gel Filtration Chromatography is one of the methods of Size Exclusion Chromatography. Size Exclusion Chromatography is an umbrella term. Other methods include Gel Permeation Chromatography where you use organic solvent as a mobile phase rather than aqueous solvent as in Gel Filtration Chromatography. Hope that helps!
Hi. Why purple color indicates the presence of protein?
its probably either the natural colour of the protein or it's dyed to make observations easier
nice
You suck at lecturing!!! Just kidding. Thanks for the concise explanation. You have a new subscriber!