This was the height of perfection in teaching.. Thank you so much Sir... for making such difficult topics easy to understand.. love all your videos ..:)
Outstanding. These concepts aren't always that complicated, but they are often explained so obtusely that it hinders comprehension. I think it is a skill to be able to communicate these things without such obstructions and you guys do this consistently.
oh man! you are absolutely beyond perfect! every time I need to have a better understanding of a subject and I search it on youtube your amazing videos are on the top of the results and they are completely enough.
Currently im studying at a medical college in Republic of Korea, and honestly you are a true life savior Thx a lot :) Ps. After watching this lecture, eating a chocolate chip cookies :D
I've watched your lecture on Gel Filtration Chromatography as well as this one and you explain the processes SO much better than my PhD University lecturer does so thank you!
Gracias por esto, que simple y facil de entender lo haces. Veo tantos comments de gente agradecida. De verdad eres un heroe, mis respetos y muchas bendiciones para ti. Saludos desde Puerto Rico.
I forgot everything of chemistry after graduation. At first, i tried to get glimpse of this because there were so many words.. However, your explanation was so clear and i already watched your other lectures. I'm really appreciated with your explanation.
You're amazing, really amazing! I swear I didn't understand a word at school (in which the teacher spent at least 1 hour explaining), and after some time when I wanted to revise I didn't understand a word either in my copies. When I discovered you made this lecture I literally jumped from happiness. After 5 mins from watching, I understood everything. Just wow... I'm really really amazed and flabbergasted at how you could explain so well. Thank you so so so much!
Thank you sir. I study molecular biology in austria and english is not my native language, but you explained this stuff better than all the german versions i´ve ever listened to. You are very talented. Tanks alot.
WOOW I have a lab today about this and I didn't get what was written so searched on UA-cam. You really explained everything so well. Thank you soso much :)))) Great video really.
Thank you so much for making these videos, they are so helpful and you are such an amazing teacher. Im able to understand these concepts clearly and concisely. I appreciate you too much, thank you!
Ak lectures is my favorite channel. by the inspiration of this channel i started my channel covering all topics regarding pharmaceutical analysis & biochemistry. i did video on ion exchange chromatography
The example and explanation is marvelous. Really well explained. Please include a bit of information about its industrial applications, its advantages and disadvantages. Thank you.
I like what you are saying and the tempo is good in your videos (i have only watched a few about chromatography)! On your previous videos about chromatography, it seems to me that you are shouting when talking. But in this video you are more calm - stay like that! :) cheers
This helps me to visualize the words I'm reading in textbooks. It really is helpful thank you so much!! That asides, who in the world hates choc chip cookies!!! They are delicious :)
While mass of any protein is not 0, suggesting that gravitational force pulls it down the column is a bit misleading to say the least. Also when you have electrostatic attractions between opposite charges in the column it is silly to suggest that anything that is electricly attracted to the stationary phase in the column will ever move down the column, unless you balance the charge between the stationary phase and that particular protein or whatever species you're trying to purify.
A very good presentation. I have though a question that may be basic. It is regarding the competition of charged salt components with attached proteins in the column medium/resin. I can understand that salts will be attracted by the opposite charge of the resin. However, because they have bigger ionic strength will they manage to disassociate the already bound proteins, break the existing ionic bond? Moreover, some proteins will be eluted but I imagine that a percentage of them will stay in column. Thank you very much in advance!
Thanks a lot for such great lecture. It is helpful and easy to understand. I would like to know, will the salt solution not have an effect on that protein?
+AK LECTURES (Andrey K) With a change in pH rather than in salt concentration, such a denaturation can also be reversed, or is it more difficult in most of cases? Great video btw
Nicely explained ! Thanks very much! One doubt - what will be the effect of pH change of the solvent ( mobile phase) on the separation / on the bead charge ??
hi andrey:) i have watched all of your videos. but for the question i have i still couldn't find the right answer. wish you could help me. the question that I'm fighting with is: from a protein mixture, we want to separate an specific enzyme with chromatography. name 2 chromatography methods that can be used so that at the end of separation we just get our desired enzyme alone separated in the final buffer? the first that i absolutely understand is the affinity chromatography. but i can not find an answer for the second method... because if we use the ion exchange like this example u made here, say our enzyme has 3 net charge on surface; if we have in mixture: 3 protein with 2 net charge, 2 protein with 3 net charge, for ex. by using a higher concentration of salt that can wash the proteins with 3 net charge, the both proteins are washed not only our desired one. it could happen also for adsorption chromatography. if in our mixture 2 proteins exist with the same adsorption behavior. also if we want to think about the partition chromatography it could also happen again, we could have in our stationary phase 2 or more proteins which have the same solubility. and by adding the buffer , the proteins with the same characteristic could be solved in buffer and eluted. i hope i didn't confused u , and you could help me.
Very nice explanation, but wouldn't protein 1 and/or 2 also interact with protein 3 in the solution (since they also have the opposite charges)? If so, could this influence the purity of an isolated protein?
this is a 1 year old question but here is the answer: yes, sometimes proteins can make associations with other proteins, opposite polarity is one of the reasons; these associations (more than one protein) can be so strong that instead of purifying single proteins the column will retain and release the protein asociation; the chemist called this proteins are "co-eluting" and the results is lower protein purity; to add a positive perspective to this: sometimes you want to purify proteins together because only together they are doing a specific reaction; so its not always bad that proteins are staying strong together :);
NOT ALL HEROES WEAR CAPES!
You're saving lives here.
Respect.
Thanks you!! Why can't teachers explain that well!?! Life saver!
qdz1005 Thank you!!! :)
THIS WAS PERFECT!!! I even clapped at the end! GOOD JOB!
+Sheri Berry lol! thank you Sheri! :)
You are a really good speaker and teacher. I understood everything 100%. Subbed. Thanks!!
This was the height of perfection in teaching.. Thank you so much Sir... for making such difficult topics easy to understand.. love all your videos ..:)
Outstanding. These concepts aren't always that complicated, but they are often explained so obtusely that it hinders comprehension. I think it is a skill to be able to communicate these things without such obstructions and you guys do this consistently.
Your videos are so helpful. Have been watching these for years and I'm almost through university. Thank you!!
oh man! you are absolutely beyond perfect! every time I need to have a better understanding of a subject and I search it on youtube your amazing videos are on the top of the results and they are completely enough.
I can't even put in words how blessed I am to discover this channel, tysm💗.You are a big time saviour !!!
Currently im studying at a medical college in Republic of Korea, and honestly you are a true life savior Thx a lot :)
Ps. After watching this lecture, eating a chocolate chip cookies :D
All of the videos that I've seen by AK Lectures are well very done and SO HELPFUL. You do a great job, thanks!!
Thanks Helen!
I've watched your lecture on Gel Filtration Chromatography as well as this one and you explain the processes SO much better than my PhD University lecturer does so thank you!
Best biology teacher in the world . No one can better than AK lecture
This was such a perfect explanation! I loved the analogy too - I wish you were my BioChem professor!
Wow, simply outstanding! You present this material so clearly and so well! Thank you so much!
Thanks Chris! glad my style of teaching is to your liking :)
Gracias por esto, que simple y facil de entender lo haces. Veo tantos comments de gente agradecida. De verdad eres un heroe, mis respetos y muchas bendiciones para ti. Saludos desde Puerto Rico.
YOU HELPED ME THROUGH 3 QUESTIONS ON MY PAST EXAM! THANK YOU!!!
I forgot everything of chemistry after graduation. At first, i tried to get glimpse of this because there were so many words.. However, your explanation was so clear and i already watched your other lectures. I'm really appreciated with your explanation.
the way you explain things is simply awesome
You are amazing and your teaching is so perfect.. Thank you for this
You're amazing, really amazing! I swear I didn't understand a word at school (in which the teacher spent at least 1 hour explaining), and after some time when I wanted to revise I didn't understand a word either in my copies. When I discovered you made this lecture I literally jumped from happiness. After 5 mins from watching, I understood everything. Just wow... I'm really really amazed and flabbergasted at how you could explain so well. Thank you so so so much!
Thank you! :) awesome to hear that!
AK LECTURES Oh, thank 'you'. I'm indebted to you. God bless you *-*
There is no explaining this any better! Great job sir! Lesson learned. Now I cannot get this out of my head…
Thank you sir.
I study molecular biology in austria and english is not my native language, but you explained this stuff better than all the german versions i´ve ever listened to. You are very talented.
Tanks alot.
Thank You SOOOOO much! I have a Biochem exam on Monday and your videos are extremely helpful and informative!
WOOW I have a lab today about this and I didn't get what was written so searched on UA-cam. You really explained everything so well. Thank you soso much :)))) Great video really.
The best and simple explanations in the world, i study microbiology and your video was very helpful, THANK YOU !!
You open my eyes ! Thank you for this hard works indeed! Love this !
Exceptional level of teaching, really grateful.
You have videos for everything I need for this course I'm having!
ما شاء الله , دقة الفيديو عالية ,, أبدعت بالشرح ,, شكرًا
this is called PERFECTION! saves a lot of time by watching this
OMG this was so good I didn't get the lecture slides but after watching your video, everything is SO clear!!!!!!!!!!!!
You always make things look very easy and simple , you are a great lecturer !!!! thanks !
Thank you so much for making these videos, they are so helpful and you are such an amazing teacher. Im able to understand these concepts clearly and concisely. I appreciate you too much, thank you!
Ak lectures is my favorite channel. by the inspiration of this channel i started my channel covering all topics regarding pharmaceutical analysis & biochemistry. i did video on ion exchange chromatography
awesome teaching style!! wont forget this concept ever!!
Chirag Mohan Sharma thank you! glad to hear the concept sunk in
Chocolate chip cookie analogy was brilliant. Just finished watching 34 videos, and you got 34 likes :) More to come.
haha thanks! :)
Thank you so much for making these lectures so simple and easy to understand!!! I'm suprised you have only around 550 views until now :P
***** Thanks for the kind words! :-)
It's alright as the years go by more people will be taking Biochemistry. He'll keep on receiving views
thanks,man! prepared for my exam now due to ur explaination 😉👍
100% understood. well done sir your all lectures are very very helpful.
Clear and quick explanation! Thank you. Just what i needed.
BEST BEST!
I was searching this answer for the Whole Day, yes whole day.
And here it was
Thank you
subscribed because of the examples you gave.. the chocolate cookies... great explanation!! helped a lot for my exams.. :)
Part with chocolate cookies - super!!
Thank you for very very nice lecture!!
Excellent video that I have seen about ion exchange chromatography, thanks a lot.❤
Explained perfectly, thank you!
Amazing explanation!!!!!! It helped me so much!!!!!
The example and explanation is marvelous. Really well explained. Please include a bit of information about its industrial applications, its advantages and disadvantages. Thank you.
Omg, this explanation was awesome! Thanks man, very helpful.
Awesome video...why ads not coming to this channel...i hv been watching this since 6 months..
Very good job ,I really like this site . Analysis made easy henceforth.
U did areally good job.... Many thanks
Your videos are so helpful . tnx
Your explanation is great
I love it 😍
Thank you so much for doing this for free.. you are awesome
Thanks much, u really great , simple teaching and clear voice , i have only one request , if u can zoom alittile on the board
such a useful lecture,, thanks
You remind me of leonardo dicaprio in wolf of wall street - your sound and mannerisms! Anyways - thank you- this is brilliant
Art of teaching.
Clearly explained 👍👍👍
Dude you've saved my life
This was beautifully explained. THANKYOU‼️‼️
perfect refresher for my job interview...😃
This was so incredibly helpful! Thank you so much
I like what you are saying and the tempo is good in your videos (i have only watched a few about chromatography)!
On your previous videos about chromatography, it seems to me that you are shouting when talking. But in this video you are more calm - stay like that! :) cheers
Perfect explanation!
Please keep doing what you are doing! You are great :)
Very educational and helpful. Thank you!!!
nice teacher.thank you very much for your work. only god will reword you
your lecture helped me a lot THANK YOU SO MUCH!!!!!!!!!!!!!!
bless your soul, thanks to you I will make it through my exam :D
Thank you kind sir, excellent review for tomorrows lab!
Really clear explanation! Thank you so much!
Thanks so much! You made it easy to understand!
Great video, and great analogy with the chocolate chip cookie kids! Thanks for this :)
This helps me to visualize the words I'm reading in textbooks. It really is helpful thank you so much!! That asides, who in the world hates choc chip cookies!!! They are delicious :)
Best explanation!
While mass of any protein is not 0, suggesting that gravitational force pulls it down the column is a bit misleading to say the least. Also when you have electrostatic attractions between opposite charges in the column it is silly to suggest that anything that is electricly attracted to the stationary phase in the column will ever move down the column, unless you balance the charge between the stationary phase and that particular protein or whatever species you're trying to purify.
Does adding the salt solution create the salting out effect? Thank you for the insightful lecture!
A JOB WELL DONE, YOU DID WELL.
A very good presentation. I have though a question that may be basic. It is regarding the competition of charged salt components with attached proteins in the column medium/resin. I can understand that salts will be attracted by the opposite charge of the resin. However, because they have bigger ionic strength will they manage to disassociate the already bound proteins, break the existing ionic bond? Moreover, some proteins will be eluted but I imagine that a percentage of them will stay in column. Thank you very much in advance!
EXCELENTE, FELICITACIONES! SE ENTIENDE PERFECTAMENTE EL INGLES!!!!
That was a really good analogy
Thanks a lot for such great lecture. It is helpful and easy to understand. I would like to know, will the salt solution not have an effect on that protein?
Sure it will. Salt solutions can denature proteins. But once you remove the salt, the protein will reform their original structure.
Thanks a lot
Lisa Touyon You're welcome Lisa :)
+AK LECTURES (Andrey K) With a change in pH rather than in salt concentration, such a denaturation can also be reversed, or is it more difficult in most of cases? Great video btw
you make everything so easy thank you soooooo much
I am the kid that will stop for the chocolate chip cookies! Well presented btw!
me too! :)
so much thank you , your videos and explanations is really helpful!!!
Medical university level, good job sir!
I understand everything now, thank you so much !!!
What if we have two negatively charged proteins and two positively charged proteins.what do we do???
OMG!!! Outstanding, you Sir!
Nicely explained ! Thanks very much!
One doubt - what will be the effect of pH change of the solvent ( mobile phase) on the separation / on the bead charge ??
hi andrey:) i have watched all of your videos. but for the question i have i still couldn't find the right answer. wish you could help me. the question that I'm fighting with is: from a protein mixture, we want to separate an specific enzyme with chromatography. name 2 chromatography methods that can be used so that at the end of separation we just get our desired enzyme alone separated in the final buffer?
the first that i absolutely understand is the affinity chromatography. but i can not find an answer for the second method... because if we use the ion exchange like this example u made here, say our enzyme has 3 net charge on surface; if we have in mixture: 3 protein with 2 net charge, 2 protein with 3 net charge, for ex. by using a higher concentration of salt that can wash the proteins with 3 net charge, the both proteins are washed not only our desired one.
it could happen also for adsorption chromatography. if in our mixture 2 proteins exist with the same adsorption behavior.
also if we want to think about the partition chromatography it could also happen again, we could have in our stationary phase 2 or more proteins which have the same solubility. and by adding the buffer , the proteins with the same characteristic could be solved in buffer and eluted.
i hope i didn't confused u , and you could help me.
This was so helpful
Now I can only think of biochem when I'm eating chocolate chip cookies. Thanks for the vid!
How would you tell where the boundaries are between the proteins as you collect them in tubes?
Thank you for this! God bless you!
Very nice explanation, but wouldn't protein 1 and/or 2 also interact with protein 3 in the solution (since they also have the opposite charges)? If so, could this influence the purity of an isolated protein?
This is something I am curious about as well. Why don't opposite charged proteins bond?
this is a 1 year old question but here is the answer: yes, sometimes proteins can make associations with other proteins, opposite polarity is one of the reasons; these associations (more than one protein) can be so strong that instead of purifying single proteins the column will retain and release the protein asociation; the chemist called this proteins are "co-eluting" and the results is lower protein purity; to add a positive perspective to this: sometimes you want to purify proteins together because only together they are doing a specific reaction; so its not always bad that proteins are staying strong together :);
What about the case of negatively charged proteins and neutral ones? Will the negatively charged reach the bottom first?
Very nice and helpful
very helpful it is....thanks sir
arjun khandelwal you're welcome! :)