This was the height of perfection in teaching.. Thank you so much Sir... for making such difficult topics easy to understand.. love all your videos ..:)
Outstanding. These concepts aren't always that complicated, but they are often explained so obtusely that it hinders comprehension. I think it is a skill to be able to communicate these things without such obstructions and you guys do this consistently.
oh man! you are absolutely beyond perfect! every time I need to have a better understanding of a subject and I search it on youtube your amazing videos are on the top of the results and they are completely enough.
I've watched your lecture on Gel Filtration Chromatography as well as this one and you explain the processes SO much better than my PhD University lecturer does so thank you!
Gracias por esto, que simple y facil de entender lo haces. Veo tantos comments de gente agradecida. De verdad eres un heroe, mis respetos y muchas bendiciones para ti. Saludos desde Puerto Rico.
Currently im studying at a medical college in Republic of Korea, and honestly you are a true life savior Thx a lot :) Ps. After watching this lecture, eating a chocolate chip cookies :D
WOOW I have a lab today about this and I didn't get what was written so searched on UA-cam. You really explained everything so well. Thank you soso much :)))) Great video really.
I forgot everything of chemistry after graduation. At first, i tried to get glimpse of this because there were so many words.. However, your explanation was so clear and i already watched your other lectures. I'm really appreciated with your explanation.
Thank you sir. I study molecular biology in austria and english is not my native language, but you explained this stuff better than all the german versions i´ve ever listened to. You are very talented. Tanks alot.
You're amazing, really amazing! I swear I didn't understand a word at school (in which the teacher spent at least 1 hour explaining), and after some time when I wanted to revise I didn't understand a word either in my copies. When I discovered you made this lecture I literally jumped from happiness. After 5 mins from watching, I understood everything. Just wow... I'm really really amazed and flabbergasted at how you could explain so well. Thank you so so so much!
Ak lectures is my favorite channel. by the inspiration of this channel i started my channel covering all topics regarding pharmaceutical analysis & biochemistry. i did video on ion exchange chromatography
Thank you so much for making these videos, they are so helpful and you are such an amazing teacher. Im able to understand these concepts clearly and concisely. I appreciate you too much, thank you!
The example and explanation is marvelous. Really well explained. Please include a bit of information about its industrial applications, its advantages and disadvantages. Thank you.
While mass of any protein is not 0, suggesting that gravitational force pulls it down the column is a bit misleading to say the least. Also when you have electrostatic attractions between opposite charges in the column it is silly to suggest that anything that is electricly attracted to the stationary phase in the column will ever move down the column, unless you balance the charge between the stationary phase and that particular protein or whatever species you're trying to purify.
I like what you are saying and the tempo is good in your videos (i have only watched a few about chromatography)! On your previous videos about chromatography, it seems to me that you are shouting when talking. But in this video you are more calm - stay like that! :) cheers
This helps me to visualize the words I'm reading in textbooks. It really is helpful thank you so much!! That asides, who in the world hates choc chip cookies!!! They are delicious :)
A very good presentation. I have though a question that may be basic. It is regarding the competition of charged salt components with attached proteins in the column medium/resin. I can understand that salts will be attracted by the opposite charge of the resin. However, because they have bigger ionic strength will they manage to disassociate the already bound proteins, break the existing ionic bond? Moreover, some proteins will be eluted but I imagine that a percentage of them will stay in column. Thank you very much in advance!
Thanks a lot for such great lecture. It is helpful and easy to understand. I would like to know, will the salt solution not have an effect on that protein?
+AK LECTURES (Andrey K) With a change in pH rather than in salt concentration, such a denaturation can also be reversed, or is it more difficult in most of cases? Great video btw
Nicely explained ! Thanks very much! One doubt - what will be the effect of pH change of the solvent ( mobile phase) on the separation / on the bead charge ??
hi andrey:) i have watched all of your videos. but for the question i have i still couldn't find the right answer. wish you could help me. the question that I'm fighting with is: from a protein mixture, we want to separate an specific enzyme with chromatography. name 2 chromatography methods that can be used so that at the end of separation we just get our desired enzyme alone separated in the final buffer? the first that i absolutely understand is the affinity chromatography. but i can not find an answer for the second method... because if we use the ion exchange like this example u made here, say our enzyme has 3 net charge on surface; if we have in mixture: 3 protein with 2 net charge, 2 protein with 3 net charge, for ex. by using a higher concentration of salt that can wash the proteins with 3 net charge, the both proteins are washed not only our desired one. it could happen also for adsorption chromatography. if in our mixture 2 proteins exist with the same adsorption behavior. also if we want to think about the partition chromatography it could also happen again, we could have in our stationary phase 2 or more proteins which have the same solubility. and by adding the buffer , the proteins with the same characteristic could be solved in buffer and eluted. i hope i didn't confused u , and you could help me.
hello I would like to ask: 1. What methods can be used to check the charge of the protein of interest before determining what type of beads (+/- charge) need to be used? 2. e.g. if somehow it can be tested that the protein of interest has a net positive charge (so we use negative-charged beads), then how can we know which elusion contains that protein, since we don't know whether it has the largest magnitude of positive charge?
NOT ALL HEROES WEAR CAPES!
You're saving lives here.
Respect.
Thanks you!! Why can't teachers explain that well!?! Life saver!
qdz1005 Thank you!!! :)
THIS WAS PERFECT!!! I even clapped at the end! GOOD JOB!
+Sheri Berry lol! thank you Sheri! :)
This was the height of perfection in teaching.. Thank you so much Sir... for making such difficult topics easy to understand.. love all your videos ..:)
Outstanding. These concepts aren't always that complicated, but they are often explained so obtusely that it hinders comprehension. I think it is a skill to be able to communicate these things without such obstructions and you guys do this consistently.
You are a really good speaker and teacher. I understood everything 100%. Subbed. Thanks!!
Your videos are so helpful. Have been watching these for years and I'm almost through university. Thank you!!
I can't even put in words how blessed I am to discover this channel, tysm💗.You are a big time saviour !!!
oh man! you are absolutely beyond perfect! every time I need to have a better understanding of a subject and I search it on youtube your amazing videos are on the top of the results and they are completely enough.
I've watched your lecture on Gel Filtration Chromatography as well as this one and you explain the processes SO much better than my PhD University lecturer does so thank you!
This was such a perfect explanation! I loved the analogy too - I wish you were my BioChem professor!
Gracias por esto, que simple y facil de entender lo haces. Veo tantos comments de gente agradecida. De verdad eres un heroe, mis respetos y muchas bendiciones para ti. Saludos desde Puerto Rico.
Currently im studying at a medical college in Republic of Korea, and honestly you are a true life savior Thx a lot :)
Ps. After watching this lecture, eating a chocolate chip cookies :D
All of the videos that I've seen by AK Lectures are well very done and SO HELPFUL. You do a great job, thanks!!
Thanks Helen!
Best biology teacher in the world . No one can better than AK lecture
There is no explaining this any better! Great job sir! Lesson learned. Now I cannot get this out of my head…
Wow, simply outstanding! You present this material so clearly and so well! Thank you so much!
Thanks Chris! glad my style of teaching is to your liking :)
the way you explain things is simply awesome
YOU HELPED ME THROUGH 3 QUESTIONS ON MY PAST EXAM! THANK YOU!!!
You are amazing and your teaching is so perfect.. Thank you for this
WOOW I have a lab today about this and I didn't get what was written so searched on UA-cam. You really explained everything so well. Thank you soso much :)))) Great video really.
I forgot everything of chemistry after graduation. At first, i tried to get glimpse of this because there were so many words.. However, your explanation was so clear and i already watched your other lectures. I'm really appreciated with your explanation.
The best and simple explanations in the world, i study microbiology and your video was very helpful, THANK YOU !!
Thank you sir.
I study molecular biology in austria and english is not my native language, but you explained this stuff better than all the german versions i´ve ever listened to. You are very talented.
Tanks alot.
Best ever teaching method👏 👏 Thanks for making this subject interesting..
Exceptional level of teaching, really grateful.
You open my eyes ! Thank you for this hard works indeed! Love this !
Thank You SOOOOO much! I have a Biochem exam on Monday and your videos are extremely helpful and informative!
OMG this was so good I didn't get the lecture slides but after watching your video, everything is SO clear!!!!!!!!!!!!
this is called PERFECTION! saves a lot of time by watching this
You have videos for everything I need for this course I'm having!
You're amazing, really amazing! I swear I didn't understand a word at school (in which the teacher spent at least 1 hour explaining), and after some time when I wanted to revise I didn't understand a word either in my copies. When I discovered you made this lecture I literally jumped from happiness. After 5 mins from watching, I understood everything. Just wow... I'm really really amazed and flabbergasted at how you could explain so well. Thank you so so so much!
Thank you! :) awesome to hear that!
AK LECTURES Oh, thank 'you'. I'm indebted to you. God bless you *-*
You always make things look very easy and simple , you are a great lecturer !!!! thanks !
Ak lectures is my favorite channel. by the inspiration of this channel i started my channel covering all topics regarding pharmaceutical analysis & biochemistry. i did video on ion exchange chromatography
ما شاء الله , دقة الفيديو عالية ,, أبدعت بالشرح ,, شكرًا
Part with chocolate cookies - super!!
Thank you for very very nice lecture!!
Clear and quick explanation! Thank you. Just what i needed.
Excellent video that I have seen about ion exchange chromatography, thanks a lot.❤
awesome teaching style!! wont forget this concept ever!!
Chirag Mohan Sharma thank you! glad to hear the concept sunk in
Awesome video...why ads not coming to this channel...i hv been watching this since 6 months..
100% understood. well done sir your all lectures are very very helpful.
Thank you so much for making these videos, they are so helpful and you are such an amazing teacher. Im able to understand these concepts clearly and concisely. I appreciate you too much, thank you!
thanks,man! prepared for my exam now due to ur explaination 😉👍
BEST BEST!
I was searching this answer for the Whole Day, yes whole day.
And here it was
Thank you
subscribed because of the examples you gave.. the chocolate cookies... great explanation!! helped a lot for my exams.. :)
Your videos are so helpful . tnx
The example and explanation is marvelous. Really well explained. Please include a bit of information about its industrial applications, its advantages and disadvantages. Thank you.
Amazing explanation!!!!!! It helped me so much!!!!!
U did areally good job.... Many thanks
Explained perfectly, thank you!
Very good job ,I really like this site . Analysis made easy henceforth.
Thank you so much for doing this for free.. you are awesome
Chocolate chip cookie analogy was brilliant. Just finished watching 34 videos, and you got 34 likes :) More to come.
haha thanks! :)
Your explanation is great
I love it 😍
Art of teaching.
Omg, this explanation was awesome! Thanks man, very helpful.
This was beautifully explained. THANKYOU‼️‼️
You remind me of leonardo dicaprio in wolf of wall street - your sound and mannerisms! Anyways - thank you- this is brilliant
nice teacher.thank you very much for your work. only god will reword you
Thank you so much for making these lectures so simple and easy to understand!!! I'm suprised you have only around 550 views until now :P
***** Thanks for the kind words! :-)
It's alright as the years go by more people will be taking Biochemistry. He'll keep on receiving views
Perfect explanation!
While mass of any protein is not 0, suggesting that gravitational force pulls it down the column is a bit misleading to say the least. Also when you have electrostatic attractions between opposite charges in the column it is silly to suggest that anything that is electricly attracted to the stationary phase in the column will ever move down the column, unless you balance the charge between the stationary phase and that particular protein or whatever species you're trying to purify.
Very educational and helpful. Thank you!!!
That was a really good analogy
Thank you kind sir, excellent review for tomorrows lab!
Thanks so much! You made it easy to understand!
This was so incredibly helpful! Thank you so much
I like what you are saying and the tempo is good in your videos (i have only watched a few about chromatography)!
On your previous videos about chromatography, it seems to me that you are shouting when talking. But in this video you are more calm - stay like that! :) cheers
Thanks much, u really great , simple teaching and clear voice , i have only one request , if u can zoom alittile on the board
such a useful lecture,, thanks
your lecture helped me a lot THANK YOU SO MUCH!!!!!!!!!!!!!!
Really clear explanation! Thank you so much!
Best explanation!
Great video, and great analogy with the chocolate chip cookie kids! Thanks for this :)
EXCELENTE, FELICITACIONES! SE ENTIENDE PERFECTAMENTE EL INGLES!!!!
you make everything so easy thank you soooooo much
Dude you've saved my life
A JOB WELL DONE, YOU DID WELL.
perfect refresher for my job interview...😃
so much thank you , your videos and explanations is really helpful!!!
Please keep doing what you are doing! You are great :)
This helps me to visualize the words I'm reading in textbooks. It really is helpful thank you so much!! That asides, who in the world hates choc chip cookies!!! They are delicious :)
Clearly explained 👍👍👍
A very good presentation. I have though a question that may be basic. It is regarding the competition of charged salt components with attached proteins in the column medium/resin. I can understand that salts will be attracted by the opposite charge of the resin. However, because they have bigger ionic strength will they manage to disassociate the already bound proteins, break the existing ionic bond? Moreover, some proteins will be eluted but I imagine that a percentage of them will stay in column. Thank you very much in advance!
Does adding the salt solution create the salting out effect? Thank you for the insightful lecture!
bless your soul, thanks to you I will make it through my exam :D
I understand everything now, thank you so much !!!
Thank you for this! God bless you!
This was so helpful
Very nice and helpful
I am the kid that will stop for the chocolate chip cookies! Well presented btw!
me too! :)
Thanks a lot for such great lecture. It is helpful and easy to understand. I would like to know, will the salt solution not have an effect on that protein?
Sure it will. Salt solutions can denature proteins. But once you remove the salt, the protein will reform their original structure.
Thanks a lot
Lisa Touyon You're welcome Lisa :)
+AK LECTURES (Andrey K) With a change in pH rather than in salt concentration, such a denaturation can also be reversed, or is it more difficult in most of cases? Great video btw
Now I can only think of biochem when I'm eating chocolate chip cookies. Thanks for the vid!
Nicely explained ! Thanks very much!
One doubt - what will be the effect of pH change of the solvent ( mobile phase) on the separation / on the bead charge ??
Medical university level, good job sir!
Thaaaank you very much. really good presentation.
Wonderful lecture thank you very much.
hi andrey:) i have watched all of your videos. but for the question i have i still couldn't find the right answer. wish you could help me. the question that I'm fighting with is: from a protein mixture, we want to separate an specific enzyme with chromatography. name 2 chromatography methods that can be used so that at the end of separation we just get our desired enzyme alone separated in the final buffer?
the first that i absolutely understand is the affinity chromatography. but i can not find an answer for the second method... because if we use the ion exchange like this example u made here, say our enzyme has 3 net charge on surface; if we have in mixture: 3 protein with 2 net charge, 2 protein with 3 net charge, for ex. by using a higher concentration of salt that can wash the proteins with 3 net charge, the both proteins are washed not only our desired one.
it could happen also for adsorption chromatography. if in our mixture 2 proteins exist with the same adsorption behavior.
also if we want to think about the partition chromatography it could also happen again, we could have in our stationary phase 2 or more proteins which have the same solubility. and by adding the buffer , the proteins with the same characteristic could be solved in buffer and eluted.
i hope i didn't confused u , and you could help me.
OMG!!! Outstanding, you Sir!
What about the chloride ions that you pour down from your salt solution. Should those be collected in a separate test tube?
hello I would like to ask:
1. What methods can be used to check the charge of the protein of interest before determining what type of beads (+/- charge) need to be used?
2. e.g. if somehow it can be tested that the protein of interest has a net positive charge (so we use negative-charged beads), then how can we know which elusion contains that protein, since we don't know whether it has the largest magnitude of positive charge?