After taking tons of Chemistry, Biology and Physics classes while attending college 50 years ago, I am drawn to your excellent lectures on a myriad of topics. You, sir, are an excellent instructor. Wish I had one like you those many years ago. I will watch every one of your videos just for the joy of it before dementia sets in.
I am SO GRATEFUL for this lesson. I'm an RN who recently switched to Oncology nursing a short while ago. I've scoured the internet and specifically UA-cam for an adequate explanation of monoclonal antibodies. I have been unable to find one until encountering yours. Thank you, and keep up the good work!
Even though I was majored in Biochemistry 20something years ago, I really forgot most of what I learned because I pursued organic chemistry in graduate school. Now I have to study biochem again because of my job and your lecture helped me alot. Thank u
Thanks a lot for your videos! You are speaking quite clear so it is not really difficult to understand you for non-native speakers as I am) I hope I will learn a lot from your channel. I'm currently a 3rd year student of St.-Petersburg State Chemical-Pharmaceutical Academy, so your lectures are useful for my studies. Thanks again, keep going
Wow! I love this lecture about turning normal cells into cancer cells for study..wish you could come over to The University of Nairobi for some lectures on Immunology..! This is a blessed effort sir.
Hi, so can the monoclonal antibody be purified directly after extracted from the body (not the cells), the same as with polyclonal antibodies as you explained in the previous one? if not, why? Thanks
It's an honor to write to you Cause you are really Awesome in teaching every single aspect and I gotta say if you wasn't I may have not been accepted to the Biology Olympiad So thank you soo much It will be Much more Awesome if We could have all (most)of ahat you said as a PDF or sth to print out and study and review.Every video takes me a lot to write down the most important things that need to be reviewed but all you say is to a large dgree important Thank you sooo much and I had a Question I'm a Biology Olympian and I wanted to know till this part of biochemistry is there any tests or Questions you suggest Cause I will only kearn and remember sth if I put it to work myself anything that I can somehow test my Understanding and See how much I remember Thank you sooooo much For your Awesome job
Great !! thank you so much .I have a question .. do you know how scientist use monoclonal antibodies in Prostate-specific Antigen test (PSA) if you know can you just briefly explain to me how ! ( I mean how they're using the antibodies to measure this antigen )
Wow fantastic job in simplifying this subject of Monoclonal treatment had no idea what great potential this treatment is this treatment will be a great revolution in medicine let’s hope it can help with Covid-19
Thank you - I still didn't understand one thing : after creating hybrid cancer-plasma cells, we still have a mixture of antibodies against different epitopes - how do you create monoclonal antibodies from the hybride cells ?
If we have the sequence of the protein(the antibody), using a virus or some other method, inject the genetic sequence of the protein into any bacteria. Then the bacteria will produce these anibodies for us.
Quick question, the hybridoma should have 4N chromosomes (2N from normal, 2N from tumor ), when these hybridoma mitosis, what will happen? Its progenitors will have 2N chromosomes? If so, if its progenitor split again, will it still have the ancestor all the hybridoma characteristics?
This video is not essential for learn to formation of mAbs becoz he did not explain the selective media and HGPRT knockout gene in Multiple Myloma cells. I think as per full understanding of hybridoma technology these things are very important for us.
sry what happens when we frezze a Cancer cell?! it is still alive continues dividing but slower?!So we can have it anytime we needed it right?That's why we Freeze those Cancerous plasma Cells
After taking tons of Chemistry, Biology and Physics classes while attending college 50 years ago, I am drawn to your excellent lectures on a myriad of topics. You, sir, are an excellent instructor. Wish I had one like you those many years ago. I will watch every one of your videos just for the joy of it before dementia sets in.
You manage to consistently maintain the world's cleanest white board.
that's not a green screen?
@Kolton Williams Same
I am SO GRATEFUL for this lesson. I'm an RN who recently switched to Oncology nursing a short while ago. I've scoured the internet and specifically UA-cam for an adequate explanation of monoclonal antibodies. I have been unable to find one until encountering yours. Thank you, and keep up the good work!
I literally jumped up with joy upon seeing a video by AK Lectures on monoclonal antibodies!!
THANK YOU SO MUCH FOR THIS.
this summarizes my 10+ page readings 👍🏻👍🏻👍🏻
Everything you say makes it so much easier to understand. Its incredible!
He helps me more than any of my teachers . He is the literal definition of best!
These videos never fail in making me understand topics. Never. Thank you
You summarized 30 heavy slides into 10 mints , thank you so much !
This is too good. Thank you!
Great video, thank you
Perfect layout, great explanation. Nice job dude.
Even though I was majored in Biochemistry 20something years ago, I really forgot most of what I learned because I pursued organic chemistry in graduate school. Now I have to study biochem again because of my job and your lecture helped me alot. Thank u
Thanks a lot for your videos! You are speaking quite clear so it is not really difficult to understand you for non-native speakers as I am) I hope I will learn a lot from your channel. I'm currently a 3rd year student of St.-Petersburg State Chemical-Pharmaceutical Academy, so your lectures are useful for my studies.
Thanks again, keep going
I love your lectures :) keep doing it!!!
Wow! I love this lecture about turning normal cells into cancer cells for study..wish you could come over to The University of Nairobi for some lectures on Immunology..! This is a blessed effort sir.
Excelent videos, they have helped me alot!!! You have a a great talent of explaining ! Keep it up :)
Fabulous! Thank you!
Thank you for making this video. You explain it better than our teacher xD
FFS, your video seems to be the only good one on monoclonal antibodies on youtube. Thank you.
Would have been better if you described the screening process for the production of Abs of interest.
Loved these videos! Very helpful. The one thing I still can't understand are the applications of monoclonal antibodies vs. polyclonal.
Thank you sir :)
Clearly explained!
Very simplified explanation . Thank you.
I always enjoy your presentation because it is understandable
Your videos are awesome :) Keep it up!
Explanations are very clear ..Thank youu
splendid.nice explanation
thanks for the nice explanation
Thank you so much 🙏🙏🙏
Many thanks , more than amazing
Thank you 😍 finally I understood 😭❤️
thank you very much sir. It is very helpful.
انت رائع فى التدريس
Great explanation for the lecture you just save my exam thank you soooooo much
you have a very good Pronunciation and explication
thank you for this video 😍
THANK YOU SO MUCH
You are amazing
Hi, so can the monoclonal antibody be purified directly after extracted from the body (not the cells), the same as with polyclonal antibodies as you explained in the previous one? if not, why? Thanks
God Bless You!
Oh thank u😇
This helped me alot✋
Thanks a lot. Merci
thanks sir, ur every videos on any topic are very clear and I got it very easily. plz upload some lecture on digestive physiology.
long live sir! you are excellent lecturer!
Well explained..!! Now I can write my exam ..😊
thank you so much sir,
nicely explained.
and subscribed :)
Nicely explained sir very helpful 👌 thank you 👍
great lecture
It's an honor to write to you Cause you are really Awesome in teaching every single aspect and I gotta say if you wasn't I may have not been accepted to the Biology Olympiad So thank you soo much
It will be Much more Awesome if We could have all (most)of ahat you said as a PDF or sth to print out and study and review.Every video takes me a lot to write down the most important things that need to be reviewed but all you say is to a large dgree important Thank you sooo much and I had a Question
I'm a Biology Olympian and I wanted to know till this part of biochemistry is there any tests or Questions you suggest Cause I will only kearn and remember sth if I put it to work myself
anything that I can somehow test my Understanding and See how much I remember
Thank you sooooo much For your Awesome job
thank u. it helps a lot :)
Thanks alot ❤️ that will help me alot in my final exam
Great !! thank you so much .I have a question .. do you know how scientist use monoclonal antibodies in Prostate-specific Antigen test (PSA) if you know can you just briefly explain to me how ! ( I mean how they're using the antibodies to measure this antigen )
thank you
How did he screen the hybridomas for the select plasma cell?
THANKS SIR FOR YOUR VERY USEFUL LECTURES
+chhaya pitre welcome!
thanks bro.
sir can u please tell me ..in mouse we inject a single type of epitope of antigen or multiple epitops
Wow fantastic job in simplifying this subject of Monoclonal treatment had no idea what great potential this treatment is this treatment will be a great revolution in medicine let’s hope it can help with Covid-19
Thank you - I still didn't understand one thing : after creating hybrid cancer-plasma cells, we still have a mixture of antibodies against different epitopes - how do you create monoclonal antibodies from the hybride cells ?
what are the screening methods?
HAi sir!! During the mass production of the monoclonal antibodies, what is the process of removing the cancerous property from the plasma cells??
If we have the sequence of the protein(the antibody), using a virus or some other method, inject the genetic sequence of the protein into any bacteria. Then the bacteria will produce these anibodies for us.
Quick question, the hybridoma should have 4N chromosomes (2N from normal, 2N from tumor ), when these hybridoma mitosis, what will happen? Its progenitors will have 2N chromosomes? If so, if its progenitor split again, will it still have the ancestor all the hybridoma characteristics?
thanks!
Harry Zhao welcome Harry
Sir please can you make a video on phage display and yeast display technology for Monoclonal antibody production ?
Hello Sir, do you have Analytical lecture just like this? thank you. this is big help for self-review board exam 🤗
please come to Sheffield Hallam University and be my lecturer!!! :) THANK YOU SO MUCHHHHH!
Daisy Bernini :-) I'd love to! and you're welcome, happy to help!
Nice mouse drawing :D
Thank u for helping me studying the exam 🙏🏽🙏🏽🙏🏽
very poggour from france
Isn't that mean polyclonal just a bunch of monoclonal antibody?
Sir, you said that plasma cells die on extracting from the body, so then how is it extracted and mixed with myeloma cells?
What I understand is that, Plasma cell has short life span can not grow further for many passages. It doesn't mean plasma cell will die immediately
Thankyou so very much for all your lectures... 😊
🙏
Y can't you share notes sir....so it might helpful for many of us...
m the same guy whose ass was saved by you in Polyclonal Antibodies. :))
The body makes at least 10 to the 18 that is one quintillion or 1 million trillion different anti bodies.
thank you so muchhhhhh
mostafa hamadi you're welcome
You having a Skype?
Yes.
can you give it to me?
No I cant.
Methodology used to create New Boston Virus ?
How you will isolate the plasma cells that produce the specific Igs? Put the antigen and test which bind in the antibodyies,? Ty
kindly read on the types of immunoassay and you will have the right answer with you.
According to my perspective one can use the immunodiffusion test.
2:10 *hits that loud
This video is not essential for learn to formation of mAbs becoz he did not explain the selective media and HGPRT knockout gene in Multiple Myloma cells. I think as per full understanding of hybridoma technology these things are very important for us.
I can't understand, because I am hindi
Don’t cough on your hand lol
So the jka antibody is a monoclonal antibody correct? I should've sold my placenta to science... the hospital probably did. Awe Jam it. 🤣
sry what happens when we frezze a Cancer cell?!
it is still alive continues dividing but slower?!So we can have it anytime we needed it
right?That's why we Freeze those Cancerous plasma Cells