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Dr. Jack Auty
Приєднався 9 бер 2013
I am a lecturer at the University of Tasmania and my videos are educational content about how the body works and how this goes wrong in disease. I am also a researcher investigating the innate immune system with a particular focus on how inflammation can contribute to disease.
Research projects
How do microplastics affect human health and the health of wildlife?
How does inflammation contribute to Alzheimer’s disease?
Developing novel drugs or re-purposing existing drugs to fight inflammatory disease.
How does the body control the inflammatory response?
If you are a national or international student interested in doing an Honours, Masters or Ph.D. research project on any of these topics please send an email to jack.auty@UTAS.edu.au for more information.
Research projects
How do microplastics affect human health and the health of wildlife?
How does inflammation contribute to Alzheimer’s disease?
Developing novel drugs or re-purposing existing drugs to fight inflammatory disease.
How does the body control the inflammatory response?
If you are a national or international student interested in doing an Honours, Masters or Ph.D. research project on any of these topics please send an email to jack.auty@UTAS.edu.au for more information.
How can we use RNA sequencing to figure out how a drug works?
RNAseq is such a powerful tool! In this video I discuss how using the awesome power of RNA sequencing we can narrow down the potential mechanism of a novel drug. The key to this method is homeostasis!
Learning objectives
Explain the challenges associated with elucidating the mechanisms of action of drugs, considering the intricate nature of drug-target interactions and the limitations of current methods.
Explain and examine utilizing RNAseq techniques to identify potential mechanisms of action for drugs, utilizing transcriptomic data to uncover gene expression changes and pathways involved.
Evaluate the significance of homeostasis in utilizing RNAseq to investigate a drug's mechanism of action. Exploring how biological systems attempt to maintain equilibrium in the presence of a drug - which by definition perturbs biological processes.
Recognize the dual role of homeostasis in regulating inhibited proteins, understanding how it can both upregulate and downregulate the expression and activity of targeted proteins in response to drug treatment.
Learning objectives
Explain the challenges associated with elucidating the mechanisms of action of drugs, considering the intricate nature of drug-target interactions and the limitations of current methods.
Explain and examine utilizing RNAseq techniques to identify potential mechanisms of action for drugs, utilizing transcriptomic data to uncover gene expression changes and pathways involved.
Evaluate the significance of homeostasis in utilizing RNAseq to investigate a drug's mechanism of action. Exploring how biological systems attempt to maintain equilibrium in the presence of a drug - which by definition perturbs biological processes.
Recognize the dual role of homeostasis in regulating inhibited proteins, understanding how it can both upregulate and downregulate the expression and activity of targeted proteins in response to drug treatment.
Переглядів: 464
Відео
How can we use evolution to discover how antibiotics work?
Переглядів 123Рік тому
Learning objectives Investigate the role of resistance evolution in unraveling the mechanism of action of a drug, exploring how the emergence of resistant strains can provide insights into the drug's target and mode of action. Describe the concept of a morbidostat and its functioning, highlighting its use in studying bacterial evolution under constant drug exposure to better understand resista...
How does Next Generation Sequencing work?
Переглядів 259Рік тому
Learning Objectives: Understand next-generation sequencing principles and technologies for high-throughput DNA or RNA analysis. Explain the importance of fragment alignment in generating complete sequences during next-generation sequencing. Compare DNA sequencing and RNA sequencing methods in terms of targets, purposes, and applications. Explore the use of relative quantity analysis in DNA sequ...
How does PCR work?
Переглядів 287Рік тому
Define DNA as the genetic material responsible for carrying hereditary information in living organisms, explaining its double-stranded helical structure and the role of the genetic code in determining protein synthesis. Explain the process of DNA extraction, including the use of techniques such as chloroform and phenol, to isolate DNA from cells or tissues, emphasizing the separation of DNA fro...
What is AI? The absolute basics!
Переглядів 302Рік тому
Learning objectives Describe how statistics can be used to make predictions by analyzing data and identifying patterns or relationships. Explain the concept of sum of squares and its application in statistical analysis, such as in a linear model. Compare how statistics and AI (gradient descent) would generate a linear model by identifying their respective approaches to the sum of squares. Expla...
What are virulence factors of Bacteria?
Переглядів 1,3 тис.Рік тому
Learning objectives Explain how PAMPs (pathogen-associated molecular patterns) can influence the pathogenicity of bacteria by activating the immune system. Describe the mechanisms by which Exotoxin S and Exotoxin U block immune function, such as by inhibiting phagocytosis. Discuss the type three secretion system, which is used by some bacteria to deliver effector proteins directly into host cel...
Bacteria as pathogens
Переглядів 326Рік тому
Learning Objectives: Describe the gram stain, shape, and pathogenicity of E. coli, S. aureus, P. aeruginosa, and C. difficile. Explain how the dose of bacteria can impact the virulence of opportunistic pathogens. Discuss how changes in the location of bacterial growth on the body can affect the pathogenicity of the bacteria. Identify various environmental sources of bacteria, including food and...
Paper breakdown: Could NLRP3 inhibitors be therapeutic in Alzheimer's disease? (3)
Переглядів 425Рік тому
In these videos were we're diving into this paper: Fenamate NSAIDs inhibit the NLRP3 inflammasome and protect against Alzheimer's disease in rodent models. Daniels MJD, Rivers-Auty J, .... Lawrence CB, Brough D, pubmed.ncbi.nlm.nih.gov/27509... Learning objectives: Gain an understanding of how Daniels et al. conducted experiments to demonstrate the therapeutic potential of NLRP3 inhibiting NSAI...
Paper breakdown: Could NLRP3 inhibitors be therapeutic in Alzheimer's disease? (2)
Переглядів 485Рік тому
In these videos were we're diving into this paper: Fenamate NSAIDs inhibit the NLRP3 inflammasome and protect against Alzheimer's disease in rodent models. Daniels MJD, Rivers-Auty J, .... Lawrence CB, Brough D, pubmed.ncbi.nlm.nih.gov/27509875/ Learning objectives: Understand the experimental techniques used by Daniels et al. to investigate the potential mechanisms of action of NLRP3 inhibitin...
Paper breakdown: Could NLRP3 inhibitors be therapeutic in Alzheimer's disease? (Figure 1)
Переглядів 861Рік тому
In this video series I'm diving into this paper: Fenamate NSAIDs inhibit the NLRP3 inflammasome and protect against Alzheimer's disease in rodent models. Daniels MJD, Rivers-Auty J, .... Lawrence CB, Brough D, pubmed.ncbi.nlm.nih.gov/27509875/ Learning Objectives: Understand the historical context and research question that led to the hypothesis presented in Daniels et al.'s 2016 paper. Gain a ...
What is a Western Blot and how does it work?
Переглядів 971Рік тому
Understanding the Principles of Western Blots: Learn the fundamental principles of Western blotting, including its purpose, procedure, and components, and how it is used to detect specific proteins. Steps Involved in Western Blots: Familiarize yourself with the step-by-step process of Western blotting, from sample preparation to protein detection, and understand the importance of each step in o...
Neuroinflammation in Alzheimer's Disease: How is Amyloid Detected by Microglia?
Переглядів 1,4 тис.Рік тому
Identify the pattern recognition receptors (PRRs) involved in detecting amyloid in the context of Alzheimer's disease. Summarize the research by Stewart et al. that elucidates the roles of CD36, TLR4, and TLR6 in Alzheimer's disease. Describe how activation of CD36, TLR4, and TLR6 leads to the release of prostaglandins and cytokines in Alzheimer's disease. Discuss the hypothesis of amyloid as a...
Neuroinflammation in Alzheimer's Disease: The Role of Neutrophils
Переглядів 932Рік тому
Learning objectives Understand the mechanisms by which neutrophils are able to infiltrate the brain. Learn how neutrophil degranulation can lead to brain damage through the release of toxic granule contents. Explore the findings and implications of research conducted by Volkman et al. and Zenaro et al. that provide evidence of the involvement of neutrophils in Alzheimer's disease-associated ne...
Neuroinflammation in Alzheimer's Disease: The Role of Microglia
Переглядів 3,3 тис.Рік тому
Learning objectives Understand how microglia drive neuronal damage through phagocytosis and reactive oxygen species (ROS) creation. Explore the role of flippase and scramblase in microglia-mediated neuronal damage. Understand how annexins can provide insights into the process of microglia-mediated neuronal damage. Learn how primary cell culture can be used as a tool for Alzheimer's research o...
Animal models of Alzheimer's disease explained!
Переглядів 2,7 тис.Рік тому
Understand how insertion of human familial Alzheimer's disease genes into rodents is the foundation of most models of Alzheimer's disease. Briefly explain the process of generating genetically engineered mice. Explain how endogenous promoter regions can be used to target gene expression to specific locations within the mouse. Describe the APP/PS1 mouse model of Alzheimer's disease and list some...
What causes Alzheimer's disease: The pathophysiology of Amyloid.
Переглядів 1,7 тис.Рік тому
What causes Alzheimer's disease: The pathophysiology of Amyloid.
What causes Alzheimer's disease: Amyloid vs Tau
Переглядів 1,9 тис.Рік тому
What causes Alzheimer's disease: Amyloid vs Tau
The brain is immune privileged: What does that mean?
Переглядів 2,7 тис.Рік тому
The brain is immune privileged: What does that mean?
What are microglia and what do they do?
Переглядів 1,2 тис.Рік тому
What are microglia and what do they do?
What cells make up the brain and what do they do?
Переглядів 711Рік тому
What cells make up the brain and what do they do?
Problems in science: Translational failure - why animal models aren't working
Переглядів 1552 роки тому
Problems in science: Translational failure - why animal models aren't working
Translational failure: What is it? and what is causing it?
Переглядів 1462 роки тому
Translational failure: What is it? and what is causing it?
What is blinding in research? The placebo effect, bias and more!
Переглядів 4192 роки тому
What is blinding in research? The placebo effect, bias and more!
What is cell culture and how does it work?
Переглядів 1,3 тис.3 роки тому
What is cell culture and how does it work?
How we know smoking causes lung cancer
Переглядів 9773 роки тому
How we know smoking causes lung cancer
Inflammation in Alzheimer’s disease: Researching from cells to people and back again.
Переглядів 3053 роки тому
Inflammation in Alzheimer’s disease: Researching from cells to people and back again.
What is silver staining and how does it work?
Переглядів 3,5 тис.3 роки тому
What is silver staining and how does it work?
What is Alzheimer's disease? And what causes Alzheimer's disease?
Переглядів 8623 роки тому
What is Alzheimer's disease? And what causes Alzheimer's disease?