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Pressure Cookers & Sterilization for Mushroom Cultivation (Plus a Low-Tech Alternative)
As you take a deep dive into the exciting world of DIY mushroom cultivation, you’ll probably want to invest early on in a pressure cooker or autoclave for sterilizing all your growth medium. There are a lot of options out there, so we made this video to help you make an informed decision, learn how they work, and consider some great alternatives to sterilization.
A pressure cooker will allow you to make your own grain spawn, agar plates, liquid culture, and bulk substrates, saving you money and helping you deepen your connection with your mushrooms.
In this detailed how-to video, we’ll discuss sterilization methods and why they’re important. Then we’ll compare the pros and cons of a variety of different pressure cookers to help you choose which one is right for you, including Instant Pot, Presto canners, All American brand sterilizers, and affordable commercial autoclaves. We’ll also talk about pressure cooker safety and offer some tips for working with mushroom grow bags.
In part 2, we’ll go over standard operating procedures, explaining how to safely use a pressure cooker for a variety of different mushroom cultivation applications. This section includes specific information for using Instant Pot and All American pressure canners.
At the end, we’ll also show you a useful method for making grain spawn and other mushroom substrates without a pressure cooker, using a form of fractional pasteurization called tyndallization. Not only is this a good budget option for the hobby grower, this technique can also help you scale up by using a bulk pasteurizer. We’ll wrap up with some ideas for how to build one and why this is a great project for off-grid mushroom cultivators.
____________________________
Made with love by Fungaia, a heart-centered, donation-based mushroom company:
www.fungaia.life/
For the best, most reliable and super affordable mushroom genetics and liquid culture, visit our favorite culture library:
True Blue Genetics
www.TrueBlueGenetics.org/?coupon=FUNGAIA
Discount code: FUNGAIA
____________________________
Recommended Pressure Cookers, Autoclaves & Parts:
Instant Pot Duo
amzn.to/3W2sUSl
(Now there's an 8-quart model too)
Vintage 21qt Presto/National Pressure Canner
ebay.us/yBsuA8
(Replacement gasket: amzn.to/3zGgtnB)
All American 941 41.5-Quart Pressure Canner
ebay.us/Q25MUr
Overpressure plug: amzn.to/3LlTTTQ
Spring-loaded Petcock: amzn.to/4f1y9dq
150L Electric Vertical Steam Sterilizer
ebay.us/evnqyo
(Get the 220V!)
Bulk Pasteurizer Parts
Hot water valve: amzn.to/3Y0naeu
Turkey fryer: ebay.us/Wz37dz
Just the burner: amzn.to/3XYFVPp
____________________________
Standard Cook Times (15 psi / 1 bar):
Liquid culture / agar media: 35-45 minutes
Grain spawn (jars): 90 minutes
Grain spawn (bags): 1 hour 45 minutes
Sawdust blocks: 2 - 2.5 hours
Cook times for Instant Pot:
LC / agar: 1 hour
Grain jars: 2 hours
Sawdust / grain bags: 3+ hours
Cook Times for Tyndallization:
Round 1: 1 hour @ 165F
Cool 24 - 48 hours
Round 2: 2 hours @ 190F
Cool 24 hours
Round 3: 2+ hours @ 190F
____________________________
Other Useful Supplies
True Blue Genetics: the best liquid culture out there
truebluegenetics.org/?coupon=FUNGAIA
This is the last genetics provider you will ever need!
Gourmet and Medicinal Mushroom Cultures from Fungaia
www.fungaia.life/shop/species/
The Fungaia Cube Kit
www.fungaia.life/shop/kits/?q=cube_kit
An organic, all-in-one grow kit for dung-lover mushrooms
Pre-Sterilized Grain Bags
www.fungaia.life/shop/fertilesubstrate/?q=7lb_grain
Pasteurized, Manure-Based Mushroom Substrate
www.fungaia.life/shop/fertilesubstrate/?q=poo_sub
Monotubs and monotub filters: Microppose
microppose.com/r?id=h776jn
Discount code FUNGAIA
____________________________
More Videos from Fungaia
Mycology Lab 101: Sterile Culture Technique
ua-cam.com/video/Cw0MzDxMKHI/v-deo.html
How to Grow Mushrooms the Easy Way:
ua-cam.com/video/LjOQeaVxmsw/v-deo.html
How to Make Grain Spawn:
ua-cam.com/video/AvWC55higVw/v-deo.html
How to make your own liquid culture
ua-cam.com/video/ybOImNKrOaY/v-deo.html
How to Make Agar Media and Pour Petri Dishes:
ua-cam.com/video/IdaQdV82tE0/v-deo.html
____________________________
Created by Paul Lynn. All rights reserved.
Made with free stock footage from Pexels.
Music by Paul Lynn.
© 2024 Alembic Enterprises LLC
Walla Walla, Washington
0:00 Intro
1:14 Background Info
3:14 Sterilization Methods
7:00 Steam Sterilization Explained
8:32 Vacuum Issue
9:23 Using Pre-Sterilized Substrates
9:58 Choosing a Pressure Cooker: Pros and Cons
16:07 Heat Source
16:55 Low-Tech Alternative
17:40 Grow Bags & Safety Tips
18:57 PART 2: How to Use a Pressure Cooker, Step-by-Step
31:00 Instant Pot
34:26 All American Pressure Canner
37:27 PART 3: Sterilizing WITHOUT a Pressure Cooker
39:28 Science of Tyndallization
40:39 Tyndallization Procedure: Times & Temps
42:38 Bulk Pasteurizer
46:32 Outro
Переглядів: 23 277

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КОМЕНТАРІ

  • @TruthISeek
    @TruthISeek 11 хвилин тому

    Love the padawan braid. Though you are a Master of Mycology!

  • @MycoVance
    @MycoVance 4 години тому

    Thanks Paul! I revisit these often to refresh memory.

  • @octoberlights8816
    @octoberlights8816 14 годин тому

    This video is lit

  • @bobbyflamez8857
    @bobbyflamez8857 День тому

    Your videos are connecting some many dots for me. Thank you for your time and effort to share the knowledge you have attained 💙

  • @BloodAsp
    @BloodAsp День тому

    For Agar, I have always used a microwave for sterilizing.

  • @BloodAsp
    @BloodAsp День тому

    heapa

  • @pelvabill
    @pelvabill День тому

    Great stuff. Thanks.

  • @tselengphala
    @tselengphala 2 дні тому

    youre like my geeky younger bro but one who made it and even more wackier. i love your videos cos i am never taking myself too seriously. you make it all fun basically. one peace peace out

  • @tselengphala
    @tselengphala 2 дні тому

    i dub you Paul 'Walker' of Fungaia, master grower and one strong with spore force. Thanks man this helps 10K.

  • @s0cializedpsych0path
    @s0cializedpsych0path 3 дні тому

    The only complaint I have is around that rat-tail....😂😂😂😂 I'm just busting balls.

  • @s0cializedpsych0path
    @s0cializedpsych0path 3 дні тому

    This is the best mushroom intro video I've ever seen.... posting on MycoMafia!

  • @c.h.1073
    @c.h.1073 3 дні тому

    the pirate impression is hilarious

  • @Sifodias111
    @Sifodias111 4 дні тому

    After watching about 6 of your videos I must say, you sir have cultivated one of the best mushroom related channels I’ve ever seen on the internet. From the fantastic detailed explanations to your wonderful humor, I can’t state enough how awesome you’re channel is!! Thank you and please keep making videos!

  • @user-vf7cv7ci6o
    @user-vf7cv7ci6o 4 дні тому

    What is the spawn to substrate ratio you used in the video?

  • @olefosshaug5565
    @olefosshaug5565 5 днів тому

    This channel is a gold mine of information

  • @compubijou
    @compubijou 6 днів тому

    From your intro, I thought you were going to show the many uses of the honey, like baking bread, only to realize at the end you were de-gassing it, lol!!

  • @3marchenko
    @3marchenko 6 днів тому

    Hey! You are awesome! The best and most thorough and educational video guides I've ever come across on UA-cam

  • @pwnzormcduck6546
    @pwnzormcduck6546 8 днів тому

    This guy is awesome. He teaches it all but the mushroom community as a whole is great. They always offer help and information. Hey man I have a envirco Mac 10 XL 2*4 flow hood. It is my first hood, how do you recommend cleaning the face of it. I got it refurbished from nothing but air. They are highly rated and known in the community. Has brand new filters and was cleaned. I just want to know how I should clean the grill of it. I know you don't want the filter material getting wet.

  • @starmantarot2871
    @starmantarot2871 8 днів тому

    10/10 video

  • @las9582
    @las9582 8 днів тому

    Thank you for the videos!! ❤❤ They're super helpful!

  • @jabs4554
    @jabs4554 9 днів тому

    BEST video i have seen yet ! Thank you

  • @Jerry-x4n7x
    @Jerry-x4n7x 10 днів тому

    Do you have any evidence or proof that adding your substrate or all of that extra stuff makes any difference? Or helps the mycelium to 'recognize it later' as you say? I'm just curious.

  • @Eric-e8d3u
    @Eric-e8d3u 10 днів тому

    When growing in a tent, use a 4" vent fan for intake and hepa filter the air. This would create a positive pressure sterile air environment for the cost of a hepa filter and fan. After pressure cooking, I put the medium directly into a monotub hot and used the heat to disinfect the monotub. Ready to inoculate. Almost every video I've seen about inoculating, people aren't using a mask. Gloves are important but, masking is too. Breathe onto a mirror, the exhaled moisture gets into and onto the material people are working with. I'd wager, this specific issue is causing infections.

  • @fed6997
    @fed6997 11 днів тому

    Just came randomly across this channel. Dude, i know a lot of fungi channels....but your content is fire!

  • @Holonet01
    @Holonet01 11 днів тому

    I use glass petris and the "no pour" technique. The condensation is definitely a thing, but once it's cool enough to be solid, I pick the dishes up, holding the top/bottom together, drain off any obvious water, then put them upside-down in a small monotub with the holes uncovered--no parafilm or plastic wrap yet. A few days like that, and the worst of the water evaporates if you're not in the jungle. Not perfect, but worth it/good enough for me. The glass petris are expensive, but you can eventually go through thousands of the plastic ones, and it's super wasteful.

  • @przemysawduda6938
    @przemysawduda6938 11 днів тому

    Super cool 🎉

  • @jonahmartin332
    @jonahmartin332 12 днів тому

    I’ve been studying this videos for a few days now. So much info, definitely have a notebook handy. Thank you for your enthusiasm, and information

  • @a.r.music4you362
    @a.r.music4you362 13 днів тому

    Love what your doing and how you're doing it

  • @mattk3654
    @mattk3654 13 днів тому

    I wish i had found your channel first, before i seen others. I could've avoided a few issues and had confidence at times i didn't.

  • @t.n.1116
    @t.n.1116 13 днів тому

    Where do you get your spawn bags with injection ports?

    • @_fungaia
      @_fungaia 9 днів тому

      Hey man, we use unicorn bags and add the injection ports. We use those little rubber furniture buttons you can buy that go on like cabinet doors. Hope this helps!

    • @t.n.1116
      @t.n.1116 9 днів тому

      @_fungaia thanks! What size spawn/grow bags do you guys do for best yields? Like 10lb, 5lb, etc? Thanks 😊

  • @BrewmasterN8
    @BrewmasterN8 14 днів тому

    Man, I cant find anyone who has a good ME to water ratio! This guy says 2% sugar...does he mean glucose or any sugar? Because ME has starches and enzymes that exponentially increase the simple sugar content. So thise 10g of DME will yield way more than 2% "sugar". My search continues 😢

  • @FainTMako
    @FainTMako 14 днів тому

    love your guide and I was really surprised by the amount of knowledge you have. Its obvious that you not only have done your research but you are passionate about the craft and you clearly have experience :) One thing I want to add as my personal opinion though is that with petri dishes its better to not let the mycelium grow to the outer edges. If I notice that my plates are almost fully colonized I will try to just use them to inoculate some bags or something but I wouldnt use them for more lab work.

    • @_fungaia
      @_fungaia 9 днів тому

      Appreciate the kind words! I'll pass the tip on up, I haven't heard of that before. Sounds worth looking into.

  • @TyphoonCoffee
    @TyphoonCoffee 15 днів тому

    I really need to try this BLAGAR recipe 😊 By the way, how much activated carbon did you add to 500ml of water?

  • @TawandaChuma
    @TawandaChuma 15 днів тому

    Wish you where in or had a base in South Africa and Zimbabwe. They a lot of small scale mushroom farmers and enthusiasts who would really like to learn from you and buy some of your equipments

  • @TawandaChuma
    @TawandaChuma 15 днів тому

    Your videos are so informative, clear and concise. Thanks

  • @Eric-e8d3u
    @Eric-e8d3u 15 днів тому

    Would vegetable glycerin or propylene glycol provide an oxygen environment enough to preserve them for longer period? ... and on a similar note what if you were to purge the storage vessel of oxygen say purchase with nitrogen or CO2 would anoxia preserve the product for longer period

  • @danielmcardle3476
    @danielmcardle3476 15 днів тому

    I'm a complete novice, but I heard that you should let steam out for like 10 mins before adding the weight. What do you think about that time period sensei?

    • @danielmcardle3476
      @danielmcardle3476 15 днів тому

      I should add that you also restored my faith in humanity yesterday, so cheers for that!

  • @weflyvr6780
    @weflyvr6780 16 днів тому

    My guy, please don’t recommend using an Instant Pot for sterilization. Even the manufacturer says don’t do it

  • @albertoangelucci7930
    @albertoangelucci7930 17 днів тому

    I love your channel! You could try to do a "honey infusion" instead of pulling the air out afterwards (vacuum the powder and suck in the honey directly from the instant pot). Mixing could be awkward, but could save you hours (i'm guessing). Anyways, thanks for the awesome content!

  • @danielmcardle3476
    @danielmcardle3476 17 днів тому

    Love your outro bro. Thank you so much for the free schooling you are sharing, and keeping advertising humorous and gentle. Mush mush love for you. 😘

  • @danielmcardle3476
    @danielmcardle3476 17 днів тому

    I really enjoy using clingwrap on petri dishes! I find it simple, effective and immensely satisfying. Cheaper and easier than parafilm.

  • @djdrack4681
    @djdrack4681 18 днів тому

    Having studied and worked in food and clinical micro labs, things to always have "to sterilize/thoroughly clean" an area: - Chlorohexidine - Bleach - Denat. Alcohol (azeotropic alcohol = better/more universal uses) - Iodine - Fungicide Specific solution - Oven, pressure cooker, bunsen, blowtorch (autoclave = +1)

    • @djdrack4681
      @djdrack4681 18 днів тому

      A Lam. flow hood = not for beginner, non-DIY ppl, or shoestring budgets. A non-DIY is also not gona be able to create a large-size, seal isolation box: complete w/ comp air tanks passing heat-sterilized (and then cooled) air...Bcuz when you start getting this quality of a iso. box, DIY-or-Die ppl r just gona make a modular, or portable lam flow hood...cuz they're 90% of the way there. +1 Items: - fresh hydrogen peroxide. As a superoxide, it can help to destroy certain cells. - A dehydrating/dessicant box = for storing certain dishes/tools. - A seal (IE vacuum) oven. Pulling vacuum + heating = almost as good as autoclave, and has diff uses. IF you're serious (or large scale setup): - Nitrogen, CO2, O2 tanks and dessicant boxes w/ hose adapters (if you gotta store stamples in a environ w/ high N2, etc environment). - UV boxes (another way to help kill lot of bacteria/viruses): storing some glassware etc in it.

  • @manusblr
    @manusblr 18 днів тому

    Can use a simple micropore tape both sides , either along with silicone works very well and cheap

  • @fitnessfoodflow3785
    @fitnessfoodflow3785 18 днів тому

    love you

  • @ukcharlie7591
    @ukcharlie7591 18 днів тому

    This information is 100% wrong! Commercial growers use cell slurries NOT liquid cultures to inoculate grain spawn! There is no quality control check with a liquid culture! You need to understand the difference between a cell slurry and a liquid culture to succesfully generate pure culture grain spawn!

  • @katkas.3066
    @katkas.3066 19 днів тому

    "Oh, what a goof I am"? 🤔

  • @fitnessfoodflow3785
    @fitnessfoodflow3785 19 днів тому

    love learning with you. Free school!!!! same vibes, good to see a fellow being on the same page :)

  • @dummkompf
    @dummkompf 19 днів тому

    I hope you had/have a lot of success with this project. I fear your target audience of budding mycologists is too small a market but I hope you prove me wrong.

  • @messyties
    @messyties 19 днів тому

    Great video! When is the breeding video coming?

  • @thraxxius
    @thraxxius 20 днів тому

    I love it! Your videos are so educational in a fun and professionally way. Thanks to you I will sterilise my old syringe and start cultivating again