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Fungaia
Приєднався 23 кві 2020
Homegrown in Walla Walla, Washington, Fungaia is a heart-centered, donation-based mushroom company, driven by deep passion and a vision for sustainable symbiosis.
Firmly rooted in a do-it-yourself ethos, we endeavor to demystify fungi and make mushroom cultivation accessible to newcomers and lifelong mycophiles alike.
We believe that fungi are the finest teachers, and can help guide us on a path toward greater personal and environmental health, food security, education, social responsibility, and joy.
Firmly rooted in a do-it-yourself ethos, we endeavor to demystify fungi and make mushroom cultivation accessible to newcomers and lifelong mycophiles alike.
We believe that fungi are the finest teachers, and can help guide us on a path toward greater personal and environmental health, food security, education, social responsibility, and joy.
Pressure Cookers & Sterilization for Mushroom Cultivation (Plus a Low-Tech Alternative)
As you take a deep dive into the exciting world of DIY mushroom cultivation, you’ll probably want to invest early on in a pressure cooker or autoclave for sterilizing all your growth medium. There are a lot of options out there, so we made this video to help you make an informed decision, learn how they work, and consider some great alternatives to sterilization.
A pressure cooker will allow you to make your own grain spawn, agar plates, liquid culture, and bulk substrates, saving you money and helping you deepen your connection with your mushrooms.
In this detailed how-to video, we’ll discuss sterilization methods and why they’re important. Then we’ll compare the pros and cons of a variety of different pressure cookers to help you choose which one is right for you, including Instant Pot, Presto canners, All American brand sterilizers, and affordable commercial autoclaves. We’ll also talk about pressure cooker safety and offer some tips for working with mushroom grow bags.
In part 2, we’ll go over standard operating procedures, explaining how to safely use a pressure cooker for a variety of different mushroom cultivation applications. This section includes specific information for using Instant Pot and All American pressure canners.
At the end, we’ll also show you a useful method for making grain spawn and other mushroom substrates without a pressure cooker, using a form of fractional pasteurization called tyndallization. Not only is this a good budget option for the hobby grower, this technique can also help you scale up by using a bulk pasteurizer. We’ll wrap up with some ideas for how to build one and why this is a great project for off-grid mushroom cultivators.
____________________________
Made with love by Fungaia, a heart-centered, donation-based mushroom company:
www.fungaia.life/
For the best, most reliable and super affordable mushroom genetics and liquid culture, visit our favorite culture library:
True Blue Genetics
www.TrueBlueGenetics.org/?coupon=FUNGAIA
Discount code: FUNGAIA
____________________________
Recommended Pressure Cookers, Autoclaves & Parts:
Instant Pot Duo
amzn.to/3W2sUSl
(Now there's an 8-quart model too)
Vintage 21qt Presto/National Pressure Canner
ebay.us/yBsuA8
(Replacement gasket: amzn.to/3zGgtnB)
All American 941 41.5-Quart Pressure Canner
ebay.us/Q25MUr
Overpressure plug: amzn.to/3LlTTTQ
Spring-loaded Petcock: amzn.to/4f1y9dq
150L Electric Vertical Steam Sterilizer
ebay.us/evnqyo
(Get the 220V!)
Bulk Pasteurizer Parts
Hot water valve: amzn.to/3Y0naeu
Turkey fryer: ebay.us/Wz37dz
Just the burner: amzn.to/3XYFVPp
____________________________
Standard Cook Times (15 psi / 1 bar):
Liquid culture / agar media: 35-45 minutes
Grain spawn (jars): 90 minutes
Grain spawn (bags): 1 hour 45 minutes
Sawdust blocks: 2 - 2.5 hours
Cook times for Instant Pot:
LC / agar: 1 hour
Grain jars: 2 hours
Sawdust / grain bags: 3+ hours
Cook Times for Tyndallization:
Round 1: 1 hour @ 165F
Cool 24 - 48 hours
Round 2: 2 hours @ 190F
Cool 24 hours
Round 3: 2+ hours @ 190F
____________________________
Other Useful Supplies
True Blue Genetics: the best liquid culture out there
truebluegenetics.org/?coupon=FUNGAIA
This is the last genetics provider you will ever need!
Gourmet and Medicinal Mushroom Cultures from Fungaia
www.fungaia.life/shop/species/
The Fungaia Cube Kit
www.fungaia.life/shop/kits/?q=cube_kit
An organic, all-in-one grow kit for dung-lover mushrooms
Pre-Sterilized Grain Bags
www.fungaia.life/shop/fertilesubstrate/?q=7lb_grain
Pasteurized, Manure-Based Mushroom Substrate
www.fungaia.life/shop/fertilesubstrate/?q=poo_sub
Monotubs and monotub filters: Microppose
microppose.com/r?id=h776jn
Discount code FUNGAIA
____________________________
More Videos from Fungaia
Mycology Lab 101: Sterile Culture Technique
ua-cam.com/video/Cw0MzDxMKHI/v-deo.html
How to Grow Mushrooms the Easy Way:
ua-cam.com/video/LjOQeaVxmsw/v-deo.html
How to Make Grain Spawn:
ua-cam.com/video/AvWC55higVw/v-deo.html
How to make your own liquid culture
ua-cam.com/video/ybOImNKrOaY/v-deo.html
How to Make Agar Media and Pour Petri Dishes:
ua-cam.com/video/IdaQdV82tE0/v-deo.html
____________________________
Created by Paul Lynn. All rights reserved.
Made with free stock footage from Pexels.
Music by Paul Lynn.
© 2024 Alembic Enterprises LLC
Walla Walla, Washington
0:00 Intro
1:14 Background Info
3:14 Sterilization Methods
7:00 Steam Sterilization Explained
8:32 Vacuum Issue
9:23 Using Pre-Sterilized Substrates
9:58 Choosing a Pressure Cooker: Pros and Cons
16:07 Heat Source
16:55 Low-Tech Alternative
17:40 Grow Bags & Safety Tips
18:57 PART 2: How to Use a Pressure Cooker, Step-by-Step
31:00 Instant Pot
34:26 All American Pressure Canner
37:27 PART 3: Sterilizing WITHOUT a Pressure Cooker
39:28 Science of Tyndallization
40:39 Tyndallization Procedure: Times & Temps
42:38 Bulk Pasteurizer
46:32 Outro
A pressure cooker will allow you to make your own grain spawn, agar plates, liquid culture, and bulk substrates, saving you money and helping you deepen your connection with your mushrooms.
In this detailed how-to video, we’ll discuss sterilization methods and why they’re important. Then we’ll compare the pros and cons of a variety of different pressure cookers to help you choose which one is right for you, including Instant Pot, Presto canners, All American brand sterilizers, and affordable commercial autoclaves. We’ll also talk about pressure cooker safety and offer some tips for working with mushroom grow bags.
In part 2, we’ll go over standard operating procedures, explaining how to safely use a pressure cooker for a variety of different mushroom cultivation applications. This section includes specific information for using Instant Pot and All American pressure canners.
At the end, we’ll also show you a useful method for making grain spawn and other mushroom substrates without a pressure cooker, using a form of fractional pasteurization called tyndallization. Not only is this a good budget option for the hobby grower, this technique can also help you scale up by using a bulk pasteurizer. We’ll wrap up with some ideas for how to build one and why this is a great project for off-grid mushroom cultivators.
____________________________
Made with love by Fungaia, a heart-centered, donation-based mushroom company:
www.fungaia.life/
For the best, most reliable and super affordable mushroom genetics and liquid culture, visit our favorite culture library:
True Blue Genetics
www.TrueBlueGenetics.org/?coupon=FUNGAIA
Discount code: FUNGAIA
____________________________
Recommended Pressure Cookers, Autoclaves & Parts:
Instant Pot Duo
amzn.to/3W2sUSl
(Now there's an 8-quart model too)
Vintage 21qt Presto/National Pressure Canner
ebay.us/yBsuA8
(Replacement gasket: amzn.to/3zGgtnB)
All American 941 41.5-Quart Pressure Canner
ebay.us/Q25MUr
Overpressure plug: amzn.to/3LlTTTQ
Spring-loaded Petcock: amzn.to/4f1y9dq
150L Electric Vertical Steam Sterilizer
ebay.us/evnqyo
(Get the 220V!)
Bulk Pasteurizer Parts
Hot water valve: amzn.to/3Y0naeu
Turkey fryer: ebay.us/Wz37dz
Just the burner: amzn.to/3XYFVPp
____________________________
Standard Cook Times (15 psi / 1 bar):
Liquid culture / agar media: 35-45 minutes
Grain spawn (jars): 90 minutes
Grain spawn (bags): 1 hour 45 minutes
Sawdust blocks: 2 - 2.5 hours
Cook times for Instant Pot:
LC / agar: 1 hour
Grain jars: 2 hours
Sawdust / grain bags: 3+ hours
Cook Times for Tyndallization:
Round 1: 1 hour @ 165F
Cool 24 - 48 hours
Round 2: 2 hours @ 190F
Cool 24 hours
Round 3: 2+ hours @ 190F
____________________________
Other Useful Supplies
True Blue Genetics: the best liquid culture out there
truebluegenetics.org/?coupon=FUNGAIA
This is the last genetics provider you will ever need!
Gourmet and Medicinal Mushroom Cultures from Fungaia
www.fungaia.life/shop/species/
The Fungaia Cube Kit
www.fungaia.life/shop/kits/?q=cube_kit
An organic, all-in-one grow kit for dung-lover mushrooms
Pre-Sterilized Grain Bags
www.fungaia.life/shop/fertilesubstrate/?q=7lb_grain
Pasteurized, Manure-Based Mushroom Substrate
www.fungaia.life/shop/fertilesubstrate/?q=poo_sub
Monotubs and monotub filters: Microppose
microppose.com/r?id=h776jn
Discount code FUNGAIA
____________________________
More Videos from Fungaia
Mycology Lab 101: Sterile Culture Technique
ua-cam.com/video/Cw0MzDxMKHI/v-deo.html
How to Grow Mushrooms the Easy Way:
ua-cam.com/video/LjOQeaVxmsw/v-deo.html
How to Make Grain Spawn:
ua-cam.com/video/AvWC55higVw/v-deo.html
How to make your own liquid culture
ua-cam.com/video/ybOImNKrOaY/v-deo.html
How to Make Agar Media and Pour Petri Dishes:
ua-cam.com/video/IdaQdV82tE0/v-deo.html
____________________________
Created by Paul Lynn. All rights reserved.
Made with free stock footage from Pexels.
Music by Paul Lynn.
© 2024 Alembic Enterprises LLC
Walla Walla, Washington
0:00 Intro
1:14 Background Info
3:14 Sterilization Methods
7:00 Steam Sterilization Explained
8:32 Vacuum Issue
9:23 Using Pre-Sterilized Substrates
9:58 Choosing a Pressure Cooker: Pros and Cons
16:07 Heat Source
16:55 Low-Tech Alternative
17:40 Grow Bags & Safety Tips
18:57 PART 2: How to Use a Pressure Cooker, Step-by-Step
31:00 Instant Pot
34:26 All American Pressure Canner
37:27 PART 3: Sterilizing WITHOUT a Pressure Cooker
39:28 Science of Tyndallization
40:39 Tyndallization Procedure: Times & Temps
42:38 Bulk Pasteurizer
46:32 Outro
Переглядів: 23 277
Відео
How to Make Mushroom Honey: Ancient Medicine, New Techniques
Переглядів 20 тис.4 місяці тому
Medicinal mushrooms are making headlines as new science confirms ancient folk wisdom. From improving cognition to healing PTSD, it seems there’s nothing fungi can’t do. But not all mushroom medicine is made equal, and the best way to know what you’re actually getting is to make your own! Mushroom honey is a gentle, holistic and wholesome way to enjoy the many healing benefits of mushrooms, and ...
How to Make Agar Media and Pour Petri Dishes for Mushroom Cultivation
Переглядів 49 тис.7 місяців тому
Once you’ve mastered the basics of DIY mushroom cultivation and you’re ready to take a deep dive into the heart of mycology, making your own agar media and petri dishes is a must. In this in-depth video, you’ll discover the secret recipe to unlock infinite nutritious growth media for your fungus. Learn how to prepare, sterilize, pour, catalogue and store your very own petri dishes. Pick up lots...
Mycology Lab 101: Agar Work, Cloning, Spores & Sterile Culture Technique for Mushroom Cultivation
Переглядів 151 тис.8 місяців тому
Master mycology lab skills with this deep-dive video about advanced sterile culture technique for mushroom cultivation! Learn everything you need to know about working with mycelium on petri dishes, making agar transfers, inoculating grain spawn and liquid culture, cloning live mushrooms and germinating and isolating new cultures from a spore print. Sterile culture technique allows us to breed ...
How to Grow Mushrooms from Scratch the Easy Way: A Step-by-Step Guide to Mushroom Cultivation
Переглядів 76 тис.9 місяців тому
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How to Use a Liquid Culture Syringe to Inoculate Grain Spawn: the First Step in Mushroom Cultivation
Переглядів 32 тис.9 місяців тому
Inoculation is the first and most sensitive step in the mushroom cultivation process, but with the right genetics and this simple technique, the process can be quick and easy. Packed with lots of helpful tips and tricks, this detailed video demonstrates how to use a liquid culture syringe to inoculate grain jars and pre-sterilized grain bags, and offers some tips for getting the most out of you...
How to Make Grain Spawn: A Deep Dive into the Heart of DIY Mushroom Cultivation
Переглядів 73 тис.10 місяців тому
Grain spawn is the key to the mushroom cultivation process. This video demonstrates how to prepare perfect grain to grow your own mushrooms from scratch at home. This simple DIY method will allow you to exponentially expand your mycelium from spores or liquid culture into many pounds of spawn in just a few short weeks. Packed with lots of helpful tips, trick and techniques, this in-depth video ...
How to Make Liquid Culture the Easy Way: A Magical Hack for DIY Mushroom Cultivation
Переглядів 87 тис.10 місяців тому
Liquid culture is the simplest, easiest, most effective and most affordable way to get started growing your own mushrooms from scratch. This video demonstrates a simple, reliable DIY method to exponentially expand your mycelium from a single liquid culture syringe into many pounds of spawn in just a few short weeks, no flow hood or special skills required. Packed with lots of helpful tips, tric...
How to Make Injection Port Jar Lids for Mushroom Cultivation
Переглядів 27 тис.10 місяців тому
Mason jars are the simplest, easiest and most reliable way to get started growing your own mushrooms at home. This video demonstrates how to modify standard canning jars to turn them into specialized mushroom cultivation containers. Learn two different methods: a super quick, cheap trick to get you started right away, and a more reliable but slightly more involved technique to help you go the d...
How to Make Liquid Culture: DIY Mushroom Cultivation Made Easy
Переглядів 449 тис.2 роки тому
Liquid culture is the easiest way to expand mycelium for mushroom cultivation, from a single agar plate into hundreds of pounds of mushrooms in just a few weeks. This video demonstrates a simple, DIY process using affordable, homemade equipment and basic sterile culture technique to brew large amounts of clean, vigorous liquid culture on a budget. Learn how to prepare nutritious media, avoid co...
Fungaia World Headquarters
Переглядів 3,8 тис.2 роки тому
Fungaia is a heart-centered, donation-based mushroom company. Built from scratch and completely DIY, our mushroom cultivation lab is nested inside the legendary Boiler Room, a decades-old indoor skate ramp that we manage as a co-op.
Fungaia Flow Hood Smoke Test
Переглядів 11 тис.3 роки тому
See the Fungaia Laminar Flow Hood in action with a smoke test. This is a powerful tool with a simple design. The Fungaia Laminar Flow Hood opens new horizons in biology, making the benefits of sterile culture technique more accessible than ever before. A fraction of the size and cost of comparable units, this compact, efficient, affordable design is now available on Kickstarter!
The Fungaia Laminar Flow Hood
Переглядів 18 тис.3 роки тому
Go with the flow into uncharted territory with this compact, efficient, affordable laminar flow hood. A laminar flow hood is a sterile air filtration device, a necessary tool for almost any task which requires total sterility. Flow hoods are used for a wide variety of applications in the biological sciences, healthcare, pharmaceuticals, nuclear technology and manufacturing.
Fungaia 2021 Summer Internship Project with Fielding & Vinny
Переглядів 2,3 тис.3 роки тому
Fungaia summer interns Fielding and Vinny describe their big project! We were lucky enough to get TWO great interns this year. I proposed a bunch of ideas, and this project was the one they were most excited to tackle. As a bonus I got them to put together this sweet vid to share a bit about their experience.
Go with the Flow (Hood) - Fungaia - Walla Walla
Переглядів 4,8 тис.3 роки тому
A portable, battery-powered laminar flow hood opens new horizons in microbiology. Fungaia is a small, DIY mushroom business with big dreams. Help us spread the word-and the spores-for the Kickstarter launch! www.fungaia.life/
Love the padawan braid. Though you are a Master of Mycology!
Thanks Paul! I revisit these often to refresh memory.
This video is lit
Your videos are connecting some many dots for me. Thank you for your time and effort to share the knowledge you have attained 💙
For Agar, I have always used a microwave for sterilizing.
heapa
Great stuff. Thanks.
youre like my geeky younger bro but one who made it and even more wackier. i love your videos cos i am never taking myself too seriously. you make it all fun basically. one peace peace out
i dub you Paul 'Walker' of Fungaia, master grower and one strong with spore force. Thanks man this helps 10K.
The only complaint I have is around that rat-tail....😂😂😂😂 I'm just busting balls.
This is the best mushroom intro video I've ever seen.... posting on MycoMafia!
the pirate impression is hilarious
After watching about 6 of your videos I must say, you sir have cultivated one of the best mushroom related channels I’ve ever seen on the internet. From the fantastic detailed explanations to your wonderful humor, I can’t state enough how awesome you’re channel is!! Thank you and please keep making videos!
What is the spawn to substrate ratio you used in the video?
This channel is a gold mine of information
From your intro, I thought you were going to show the many uses of the honey, like baking bread, only to realize at the end you were de-gassing it, lol!!
Hey! You are awesome! The best and most thorough and educational video guides I've ever come across on UA-cam
This guy is awesome. He teaches it all but the mushroom community as a whole is great. They always offer help and information. Hey man I have a envirco Mac 10 XL 2*4 flow hood. It is my first hood, how do you recommend cleaning the face of it. I got it refurbished from nothing but air. They are highly rated and known in the community. Has brand new filters and was cleaned. I just want to know how I should clean the grill of it. I know you don't want the filter material getting wet.
10/10 video
Thank you for the videos!! ❤❤ They're super helpful!
BEST video i have seen yet ! Thank you
Do you have any evidence or proof that adding your substrate or all of that extra stuff makes any difference? Or helps the mycelium to 'recognize it later' as you say? I'm just curious.
When growing in a tent, use a 4" vent fan for intake and hepa filter the air. This would create a positive pressure sterile air environment for the cost of a hepa filter and fan. After pressure cooking, I put the medium directly into a monotub hot and used the heat to disinfect the monotub. Ready to inoculate. Almost every video I've seen about inoculating, people aren't using a mask. Gloves are important but, masking is too. Breathe onto a mirror, the exhaled moisture gets into and onto the material people are working with. I'd wager, this specific issue is causing infections.
Just came randomly across this channel. Dude, i know a lot of fungi channels....but your content is fire!
I use glass petris and the "no pour" technique. The condensation is definitely a thing, but once it's cool enough to be solid, I pick the dishes up, holding the top/bottom together, drain off any obvious water, then put them upside-down in a small monotub with the holes uncovered--no parafilm or plastic wrap yet. A few days like that, and the worst of the water evaporates if you're not in the jungle. Not perfect, but worth it/good enough for me. The glass petris are expensive, but you can eventually go through thousands of the plastic ones, and it's super wasteful.
Super cool 🎉
I’ve been studying this videos for a few days now. So much info, definitely have a notebook handy. Thank you for your enthusiasm, and information
Love what your doing and how you're doing it
I wish i had found your channel first, before i seen others. I could've avoided a few issues and had confidence at times i didn't.
Where do you get your spawn bags with injection ports?
Hey man, we use unicorn bags and add the injection ports. We use those little rubber furniture buttons you can buy that go on like cabinet doors. Hope this helps!
@_fungaia thanks! What size spawn/grow bags do you guys do for best yields? Like 10lb, 5lb, etc? Thanks 😊
Man, I cant find anyone who has a good ME to water ratio! This guy says 2% sugar...does he mean glucose or any sugar? Because ME has starches and enzymes that exponentially increase the simple sugar content. So thise 10g of DME will yield way more than 2% "sugar". My search continues 😢
love your guide and I was really surprised by the amount of knowledge you have. Its obvious that you not only have done your research but you are passionate about the craft and you clearly have experience :) One thing I want to add as my personal opinion though is that with petri dishes its better to not let the mycelium grow to the outer edges. If I notice that my plates are almost fully colonized I will try to just use them to inoculate some bags or something but I wouldnt use them for more lab work.
Appreciate the kind words! I'll pass the tip on up, I haven't heard of that before. Sounds worth looking into.
I really need to try this BLAGAR recipe 😊 By the way, how much activated carbon did you add to 500ml of water?
Wish you where in or had a base in South Africa and Zimbabwe. They a lot of small scale mushroom farmers and enthusiasts who would really like to learn from you and buy some of your equipments
Your videos are so informative, clear and concise. Thanks
Would vegetable glycerin or propylene glycol provide an oxygen environment enough to preserve them for longer period? ... and on a similar note what if you were to purge the storage vessel of oxygen say purchase with nitrogen or CO2 would anoxia preserve the product for longer period
I'm a complete novice, but I heard that you should let steam out for like 10 mins before adding the weight. What do you think about that time period sensei?
I should add that you also restored my faith in humanity yesterday, so cheers for that!
My guy, please don’t recommend using an Instant Pot for sterilization. Even the manufacturer says don’t do it
I love your channel! You could try to do a "honey infusion" instead of pulling the air out afterwards (vacuum the powder and suck in the honey directly from the instant pot). Mixing could be awkward, but could save you hours (i'm guessing). Anyways, thanks for the awesome content!
Love your outro bro. Thank you so much for the free schooling you are sharing, and keeping advertising humorous and gentle. Mush mush love for you. 😘
I really enjoy using clingwrap on petri dishes! I find it simple, effective and immensely satisfying. Cheaper and easier than parafilm.
Having studied and worked in food and clinical micro labs, things to always have "to sterilize/thoroughly clean" an area: - Chlorohexidine - Bleach - Denat. Alcohol (azeotropic alcohol = better/more universal uses) - Iodine - Fungicide Specific solution - Oven, pressure cooker, bunsen, blowtorch (autoclave = +1)
A Lam. flow hood = not for beginner, non-DIY ppl, or shoestring budgets. A non-DIY is also not gona be able to create a large-size, seal isolation box: complete w/ comp air tanks passing heat-sterilized (and then cooled) air...Bcuz when you start getting this quality of a iso. box, DIY-or-Die ppl r just gona make a modular, or portable lam flow hood...cuz they're 90% of the way there. +1 Items: - fresh hydrogen peroxide. As a superoxide, it can help to destroy certain cells. - A dehydrating/dessicant box = for storing certain dishes/tools. - A seal (IE vacuum) oven. Pulling vacuum + heating = almost as good as autoclave, and has diff uses. IF you're serious (or large scale setup): - Nitrogen, CO2, O2 tanks and dessicant boxes w/ hose adapters (if you gotta store stamples in a environ w/ high N2, etc environment). - UV boxes (another way to help kill lot of bacteria/viruses): storing some glassware etc in it.
Can use a simple micropore tape both sides , either along with silicone works very well and cheap
love you
This information is 100% wrong! Commercial growers use cell slurries NOT liquid cultures to inoculate grain spawn! There is no quality control check with a liquid culture! You need to understand the difference between a cell slurry and a liquid culture to succesfully generate pure culture grain spawn!
"Oh, what a goof I am"? 🤔
love learning with you. Free school!!!! same vibes, good to see a fellow being on the same page :)
I hope you had/have a lot of success with this project. I fear your target audience of budding mycologists is too small a market but I hope you prove me wrong.
Great video! When is the breeding video coming?
I love it! Your videos are so educational in a fun and professionally way. Thanks to you I will sterilise my old syringe and start cultivating again