cDNA Libraries and Expression Libraries | MIT 7.01SC Fundamentals of Biology
Вставка
- Опубліковано 8 тра 2012
- cDNA Libraries and Expression Libraries
Instructor: Eric Lander
View the complete course: ocw.mit.edu/7-01SCF11
License: Creative Commons BY-NC-SA
More information at ocw.mit.edu/terms
More courses at ocw.mit.edu
actually Eric Lander is so good that I watch these lectures for entertainment (and learning of course)
When that student said “Human cell”, i was like, nah, must be bacterial or yeast cell, then out of my expectation, the teacher went “That’s interesting, let’s put it as an option”. That is the moment i saw the light of science, education and my ignorance... Great lecture
and the difference between a good and bad professor
The way he filled the classroom with positivity and curiosity together is just awesome
Oh my god, I wish I had a teacher like this! Perfectly understandable AND funny? It doesnt feel like studying anymore :D
Same. i wish my lecture actually care if student understand the material or not. all the care about if getting to the of their prepared slides that's all
That's what I call teaching! I wish I had more incredible professors like this ! Thanks a lot !
He does a great job of making it interesting
Well done. I almost got teary-eyed. I miss college!
Amazing! Wish that was my professor!!!
eric lander saved my sorry self today after i zoned out in class thank you based god eric lander
I have to write an essay on this, and was unsure of the steps. Thanks for the clarification, i wish my lecturers were this good at explaining!
Incredible teacher, MIT students have lot of chance
I agree with u guys.. I like the way he taught that, he made it look so simple. I wish to learn more from him...
he is the ultimate teacher of Biology.
Wonderful Lesson a midnight before my exams
This was wonderful! Thanks very much. Extremely helpful and engaging.
The best teacher ever...
This guy is great. Pretty fast for my brain but his energy is infectious. Jealous of those students!
Wonderfully presented!
The lecture is a legend.
This is my permanent teacher
This guy makes sense to me.What a wonderful lessons....Thanks% you are a mazing
what is great professor, i wish to learn from one like this
such a cool professor
Great teacher!
Really a great teacher!
Really helpful, thanks so much
Amazing Prof.!!!!
I'm doing good with this time thanks to your help
its in the catalogue. love from india
Wow excellent lecture😊
perfect lecture
U r amazing teacher 😃
these Master is awesome... hahah I am working in 4 sec the daily question .great
oui exacte
@ 7:14 - 7:32
One question, I suppose we're growing Beta Globin cells for the purpose of using it?
If the antibody sticks on the Beta Globin cells, these cells become un-usable?
Anyone who knows the answer, pls share your knowledge.
I suppose we'd grow these cells in order to use the beta globin itself (as in the example of insulin at the end). In that case, attaching the fluorescent antibody to the cells would kill them in the process because, as he explained, you'd first have to destroy their membranes and spill out their content. Thus the cells die, but the beta globin is still usable.
thanks a lot.
now I have some questions.
1) how do you destroy their cell membrane without denaturing the beta Globin protein?
2) and how do you isolate the beta Globin protein from the mess?
looking forward to your reply.
Leon, Ye Lin Kaung
1) There are many ways to break down the cell's membrane (a process called "lysis"): repeated freezing and thawing, sonication, homogenization by high pressure, filtration, or permeabilization by organic solvents. The method of choice depends on how fragile the protein is and how sturdy the cells are.
Leon, Ye Lin Kaung
2) This usually depends on the mass of the protein. Anything that weighs more than 10 kDa can simply be extracted and purified by a centrifuge (either zonal or differential). Beta globin is just under 16 kDa, so that should do the thing.
Leon, Ye Lin Kaung
Otherwise you have to rely on other techniques that target other characteristics of the protein: electrophoresis (separates in function of charge), chromatography (in function of mass, charge or strength of its bonds) or simply an enzyme that binds to certain proteins
He's preparing them to be inventors.
I wish the opportunity to one day study at MIT
sounds like we have the same professor :P, except mines with a french accent
I would like to have such a teacher.
Chciałbym mieć takiego nauczyciela.
sun everything comes from it, photosynthesis, next next. and d 4 terrestrial plan will take when suns color is our red.
I dont get this. If you wanna clone a protein from human gemone, you simply design a specific pair of primers and amplify. Why bother fluorescent ab selection?
In my understanding, flourescnt antibody will bind to the beta globin protein in the filter paper and this would tell us which cell or colony actually expressed the beta globin gene.
Learning in the United States seems so easy. In India, in the same amount of time, you need to process five times more data due to the competition and resulting higher floor of knowledge.
How so? :O
Pls upload elaborately about chromosome walking
bookmark 11-23-23
Make a Protein! ;)
flawless teaching...
but dumb class nobody answers the questions
Right? This is MIT right? I go to UTEP (not even a tier 1 university) and I knew how to make this contruct right away and I don't even have my bachelors yet.
Jonathan vazquez
This is 7.01 - an INTRODUCTORY biology course.