Construction of cDNA library | Synthesis of cDNA | rDNA Technology | Bio science
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- Опубліковано 5 вер 2024
- Construction of cDNA library | Synthesis of cDNA | rDNA Technology | Bio science
A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, of which constitute some portion of the transcriptome of the organism and are stored as a "library". cDNA is produced from fully transcribed mRNA found in the nucleus and therefore contains only the expressed genes of an organism. Similarly, tissue-specific cDNA libraries can be produced. In eukaryotic cells the mature mRNA is already spliced, hence the cDNA produced lacks introns and can be readily expressed in a bacterial cell. While information in cDNA libraries is a powerful and useful tool since gene products are easily identified, the libraries lack information about enhancers, introns, and other regulatory elements found in a genomic DNA library.
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You well explained bro. Before this video I can't understand this topic but after watching your video . Everything understood. Thank you so much
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The name reverse transcriptase is due to synthasise of DNA from RNA not for 3' to 5 '
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Good explanation, but for the formation of mRNA only transcription occurs, translation is not done
And reverse transcriptase enzyme synthesizes DNA from RNA in 5' to 3' direction
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Best videos on these topics. Thankyou for putting these here :)
My pleasure
Thank you so much bro for clear cut explanation
You need to clear your concept about synthesis direction movement of polymerases 👍🏻👍🏻👍🏻
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Bro u r explanation is too good
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Good explanation bro I watched this vdo before my semester exm without knowing the concept. Now I know about cDNA construction . Thankyou
Can you please explain DNA shot gun method!!? PLEASE
very good information
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Very well explained sir
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It's very useful thank you for giving this info great job 🥳🥳
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Nice explanation
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Can I get the pdf of that notes , please
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easily understood thank you
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Thank you, clear concept❤
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Nice lecture 👌
Can you help me in learning for msc zoology entrance exam?
Where is the link...not found in the description box
Waoww totally cleared
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Thank you bro for Crystal clear explanation could you pls provide the whatsapp link to join the group .I want the notes for this topic
As it moves to 5 prime end will form hair pin loop
Sir plz upload retroviral method ....i have to prepare that for my exams ..because of lockdown i was unable to attend any tuition or class from anyone .... Probably my offline exams gets started from November 20.. i have to prepare other subjects toooo ..plz sir help me 🙏
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Bhaii restriction mapping of DNA fragments and map construction topic par banaiye
Yeah already uploaded
I can't join the what's app group bcz it's full.....
those are not "cloned plasmids", it called as recombinant DNA
They are also called as cloned plasmids
Sir, can you plz resend the notes of this topic in the group... I just joined it
How can I get these notes
Thymine nucleotide
Zabr 10 yar...
Love from pakistan
Thanks
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Good concept... kindly can I get the link of wp group.
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S 1 nuclease cleave
Oligo dt primer
Brother me what's app group is full add kavdaniki avdamle
Are the cDNA fragments single stranded or double stranded?
Double stranded
Hi sir, I just wanted to join in your WhatsApp grp for notes purpose but it showing unable to join It is full plz do something
Exons r coding sequence
Dna polymerase
Where do I find whatsapp link for the notes?
Sir Plz share what's app link for notes
Reverse transcriptase
Alkaline naoh
5:04
Talking slowly 🙏
Sir whatsaap no. ?
Bro whats app group link bro
Bhaii pdf milega iska
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Spilicing
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