MTT Assay

( www.abnova.com ) - A demonstration on the procedure of using MTT assay to assess the viability and the proliferation of regular cells with absorbance detection at 595nm is shown. This colorimetric assay measures the reduction of yellow 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) by mitochondrial succinate dehydrogenase. More videos at Abnova www.abnova.com

[@spectroxis6418]

Taking my baby steps into research, your videos have helped a lot!

[@user-ke1el7pm2d]

Very nice videos.. i am very thankful.. Abnova thanks a lot

[@rasoihargharki342]

very good video..with nice explaination of steps..

[@MrE03]

Music sure makes everything exciting.

[@vro20]

The music is very therapeutic.

[@flowerblossom4131]

Thank you

[@charlesahweyevu]

Must be very nice with the multichannel pipetter >_>. I have to use the single ones T_T

[@auto2829]

I feel you're pain, had to do the same at my university.

[@TIB5]

lool im the same!!!! and i have to use the 96-wellplate formatting...

[@bitoyau]

This was great, I've been looking for "sirt1 mitochondrial biogenesis" for a while now, and I think this has helped. Have you ever come across - Miyason Mitochondria Masker - (should be on google have a look ) ? Ive heard some unbelievable things about it and my partner got great results with it.

[@jasonlivinghouse330]

The music is terrific

[@shailashinde7187]

nice video

[@valeriedavidson1407]

Thanks for the video, I am having trouble plating my cells evenly across the 96-well plate. Does anyone have any tips? Thanks!

[@tolunaytemiz]

instead of a 96-well plate, what about using h.g. wells for culture?

[@amygeraghty385]

is cross contamination a possibility?

[@Vunami]

Isn't the absorbance supposed to be around 400-450nm for purple?

[@kumarshivam5197]

very nice

[@falahhassan4422]

thanks for this jop

[@shoukatwali4198]

can someone please tell me the same process is for candida species ?? i try same but donot remove medium and add dmso ,,i use mtt with pms solution but did not get the desire color

[@aishwaryalaxmi2173]

Great 👍

[@priyankadas8790]

Nice👏

[@hans6550]

can i do my mtt not inside the bisafety cabinet?

[@MuhammadShahid-mj2zs]

is any one know in running a MMT cytoxicity assay in addition to prodrug what elas molecules would you test

[@antis.1974]

Which is the first well ? Alphabet H or A ?

[@andrea-qw5my]

Do I need to resuspend after adding MTT solution?

[@aondohembanege9551]

Hi, thanks for the video. Please, I have a question: I will like to ask a question about cell treatment experiment with a test drug for 72 h. In such an experiment, do I have to add my drug once and incubate for 72h then check the cell viability or I have to remove the drug solution and replace with the same drug and concentration after each 24h to avoid wrong readings due to degraded drug in wells or evaporation? Please any other person reading the comments can also reply me if you have the correct answer to my question. Thank you

[@dr.hannibal8338]

why we used DMSO?

[@vasudhapriyadharshni4570]

What are the apparatus used here. Would like to buy the same. Can someone list it

[@atozatoz952]

Multi-well pipette

[@amirulfaez8437]

why do we need to discard medium and add DMSO?

[@Vanillaplayers]

To my understanding it is to lyse the cells to release the formazan produced inside the cells, otherwise we couldn't read the absorbance caused the substance

[@nahidsabahaldinsalihi5970]

I know this is too late but I cant help it, some do remove the media because it has a color which may affect the results as MTT is a colorimetric assay.

[@kadeshzalander5662]

Some people do not discard the medium and just add DMSO or SDS and make a correction for the absorbance, just leaving some wells with medium and making a subtraction of this value to the rest of the absorbances obtained.

[@julesteuwen]

To solubilize the crystals that are formed in cells

[@user-fh8eq9of6g]

We need complete kit for MTT assy can anyone send details of vendor

[@chetanpachauri530]

#BiologyofDiagrams.. 👍