MTT assay | Cell Viability and Cytotoxicity determination using MTT assay
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- Опубліковано 5 лют 2025
- This video talks about MTT assay and answers following questions like:
How MTT assay is done?
How does MTT assay determine cell viability?
What is MTT assay cancer?
What is MTT assay used for?
What are advantages and disadvantages of MTT assay?
How do you use MTT test?
Why is determining cell viability important?
This was awesome, you took the major concepts and gave a quick overview of how they connect which makes it easier to understand
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i love you king i was so confused trying to write my lab report for 2nd year biochem, but this saved me and i understand the content so much more now
First of all, thankyou for making this content. It helps me a lot during uni 😭👍🏻 You make it so simple and easy to understand
Please share my channel with your friends and help me to reach big audience..lots of love from India
Very helpful video (animations). Helped me clear my concepts. God bless you❤
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waooo unbelievable~you really made this video so quickly.I really appreciate all your effort. Well done sir!
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Don't forget to leave your suggestions
@@animatedbiologywitharpan sure sure,I will try my best to share your working for many people .
This video is really helpful. Thank you for your wonderful presentation, it really helped me in my research study
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Ur videos are really a way better and lucid than other youtubers 🧡❤
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Very helpful video sir..Thankuu
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Thank u.. clear and very beauty explain
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Such an awesome video..thank you so much💓💓
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Amazing explanation sir.thank you so much sir.
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Honestly this was so much clear and helpful ! thank you very much you are amazing !!
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very clear and simple explanation. love it
:)
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Arpan, your videos are incredible, please keep going!
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Thank you for making this video. it really helps.
Please share my channel link with your friends and help me to reach big audience
very useful thankyou sir
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Very helpful video, Thanks
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Nice lecture sir.. Useful 😇
Bro, amazing animations and explained very well!
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Very well-detailed video!
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Thank u for such good concepts
Appreciate it
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@@animatedbiologywitharpan ofcourse man I will . Keep up the good content
Thank you so much sir for your time and effort :D a very interesting video and it helps me a lot to do my research in my undergraduate study.
Really glad to hear that my contents helped you 😊
Great sir.Thank you so much
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@@rajarshibose3318 checkout our techniques in biology playlist.
That "Hellow!" at the start of your videos is my energy drink these days. Haha!
Ha ha ha ha 😂
Nice lecture ,
Sir plzzzzzz explained cell revival,cell subculturing ,cell culture , splitting and passaging etc Also with protocol.
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Cell culture playlist ua-cam.com/play/PLKtiwIJ8Q7rpNrZRxzBgoH2Q6oz4bJwg2.html
Thanx sir for your reply .
Keep it up for making such a nice videos
Plz recommend any good book for cell culture protocol related.
We need more of such clips ... very informative and concise
please share with your friends
Great thanks very much
Thank you for this video.
I need to have cytotoxicity studies done for several samples of mercury sulphide on healthy cell lines of liver and kidney. Can you help me on where this can be done and what may be the approximate cost? Thanks in advance.
Please tell me cells seedling calculation
Thank you
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Nice video :)
Where are you get the animations (the little pictures)? Is there a website link?
I make it using adobe illustrator
But let me tell you similar graphics are
Available at www.biorender.com
Sir can you make a video on cell invasion and migration assay
I will try my best
I would like to ask if the reduction of tetrazolium takes place in the cytoplasm or in the mitochondria, i.e. the dye also penetrates into the mitochondria
Thank you so much !!!
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Can i use this technique to measure cell viability after autolysis of E. Coli??
Thank you so much 😭👍🏻
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Please make vedio on Oecd guidelines 407, 408, 409, 410 and on Neutral Red assay
Hello! so does MTT and LDH assays both use yellow tetrazolium salts that get converted into formazan? why is formazan produced different colours? is it bc in mtt the tetrazolium salt contains bromide (MTT) and in LDH - iodine (INT)?
Must we wait 2 hours after adding DMSO?
Is it a biochemical test
what did you put in the control and treatment
Could you please elaborate ? I did not understand
Thank you so much Sir... Sir can you please explain the Annexin V fitc experiment
Thanks for your suggestion I will make a video on that
thank you sooo much
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Thanks arpan
Could you please help me by sharing my contents with your friends
How we can generate standard curve or
how we know that how much cell are viable in or standard?
🤔
thank you :)
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Hi, thanks for the video. Please, I have a question: I will like to ask a question about cell treatment experiment with a test drug for 72 h. In such an experiment, do I have to add my drug once and incubate for 72h then check the cell viability or I have to remove the drug solution and replace with the same drug and concentration after each 24h to avoid wrong readings due to degraded drug in wells or evaporation? Please any other person reading the comments can also reply me if you have the correct answer to my question. Thank you
It depends on bioavailability. If the drug is degraded within a day then it has to be added multiple time.
Thank you very much for taking out time to respond to this my question here. I really appreciate it @animatedbiologywitharpan
thanks for the kind words@@aondohembanege9551
thank soo much
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Very nice and easy explanation. Pls let me how to making this slide in ppt?
Thanks for your compliments
Cat we use MTT assay for investigation of IC50 ?
@@panuwitkamwong3108 yes yes, it can be used
On which website I will find this information in written ?
www.sigmaaldrich.com/IN/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/cell-counting-and-health-analysis/cell-proliferation-kit-i-mtt
What are the material and chemicals required for this experiment?
Refer to this websote for further details
ua-cam.com/video/wJIUmE5RSNo/v-deo.html
When drug is added into it
Could you please elaborate
Hi, I have a question regadring with MTT assay😃
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Which app did you use?
Powerpoint
@@animatedbiologywitharpan Thankyou so much
It has really helped me a lot in understanding the test report of my research samples. Please let me know in which stage does the treated sample extract come in contact with the cells
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Cancer, apoptosis, metabolic activity of cell- MTT assay
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Dickinson Green
@@BettyHall-m6c Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Y we call it MTT ?
Because of the dye which is used in this procedure,"Tetrazoleum" which is short form of 3-(4,5-diMethylThiazol2-yl)-2,5-diphenylTetrazoluem bromide simply, MTT
Because Umtiti
Christa Camp
Sir can you tell me how i find IC50 value from the standard curve of this assay
Edna Shoals
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Koch Glen
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Jolie Village
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Milo Haven
Senger Hollow
Can you tell me what is the function of DMSO?
DMSO is added at the end of the reaction to dissolve the formazan crystals formed from the reaction. Otherwise, you can not properly measure the color density of the reaction. DMSO is added only after incubation with MTT dye.
but DMSO has an antibacterial effect, will this ruin the experiment when using e.coli strains for example ?
Devonte Square
can u please share your slides
you can buy it @5$ , please emailis at arpanparichha1994@gmail.com for more details