Cell proliferation and cytotoxicity assay

Поділитися
Вставка
  • Опубліковано 11 гру 2024

КОМЕНТАРІ • 25

  • @zunikaamit
    @zunikaamit 4 роки тому +4

    Hi Julia, your video shows how to use the product with suspension cells. How about the adherent cells?

    • @michaeljones2781
      @michaeljones2781 4 роки тому

      Hi Zunika, Orangu works great with adherent cells.

  • @shaymaabalamand
    @shaymaabalamand 6 місяців тому

    Thanks so much.. very helpful ❤ Julia

  • @FA-oz4cd
    @FA-oz4cd 2 роки тому +1

    Hi Julia, can you explain how do you generate your calibration curve?

  • @scholarshipnetworkguideoff7988

    Great work done, I am PhD Scholar in China, Wish to work with you

  • @twinbrothers111
    @twinbrothers111 3 роки тому

    Thanks Jolia
    Plz tell me how you determine LD50 plz

  • @fran16justo
    @fran16justo 2 роки тому

    Really nice!!

  • @gogomahmoud6807
    @gogomahmoud6807 2 роки тому

    From the videos i watched .the color converted from yellow to purple and here is become orange why?

    • @kosheeka
      @kosheeka 5 місяців тому

      It depends on the dye you are using for your cell cytotoxicity assay.

  • @teferaworku8842
    @teferaworku8842 5 років тому +1

    Hi Julia I want to use the protocol that you have been demonstrated last time using column chromatography would you please! share me the the type of chemicals(reagent), column chromatography. And also would you send me the protocol and the required chemicals and instrument for the method. Thank you Julia

    • @CellGS
      @CellGS  5 років тому

      Hi Tefera,
      The Exosome Isolation range protocols can be found here: www.cellgs.com/items/exosomes/exospin.html.
      Please do not hesitate to email us at tech@cellgs.com if you have any further questions. Thank you!

  • @luisp.9710
    @luisp.9710 2 роки тому

    Hi. Can this kit be used after 6 months (shelf life) and get reliable results?

    • @michaeljones2781
      @michaeljones2781 2 роки тому +1

      Hi Luis, Yes, the kit will still be reliable after 6 month's storage

  • @biotical7697
    @biotical7697 3 роки тому

    Hi julia
    I Tried orangu assay for HCC cell line(Hep3B) , i seeded almost 7500 cells in per well(96 well) and it incubated , but it doesn't work i tried many times but i didn't get result do you have any suggestion?

    • @michaeljones2781
      @michaeljones2781 3 роки тому

      Hi I'msadaf, please contact tech@cellgs.com for support

  • @rjkim5000
    @rjkim5000 3 роки тому

    Nothing on quantified data analysis and results interpretation?

    • @michaeljones2781
      @michaeljones2781 3 роки тому

      Please have a look here. www.cellgs.com/exosomes.htm where we show examples of data generated using exosome purified using Exo-spin.

  • @dr.senthilkumarkalyanasund6099
    @dr.senthilkumarkalyanasund6099 3 роки тому

    Concentration in 100microlitres of Sample is?.. Reply...please... thanks

    • @CellGS
      @CellGS  3 роки тому

      Hi Senthilkumar, you can read the protocol here www.cellgs.com/products/oranguand8482--cell-counting-solution.html

  • @45bounty
    @45bounty 4 роки тому

    thk's so much..very helpful -)

  • @lailatarar5885
    @lailatarar5885 4 роки тому

    Hi julia can you tell me in detail about PFC assay mean to say how to perform it

    • @CellGS
      @CellGS  4 роки тому

      Hi Laila,
      The protocol for Orangu can be found in the following user guide: cellgs.e2ecdn.co.uk/Downloads/Orangu_User_Guide.pdf
      Please do not hesitate to contact us at tech@cellgs.com if you have any further questions. Many thanks.

  • @dr.l.v.prasaddiagnostics6035
    @dr.l.v.prasaddiagnostics6035 2 роки тому

    WHAT IS THE DIFFRENCE BETWEEN Cell proliferation and cytotoxicity assay

    • @michaeljones2781
      @michaeljones2781 2 роки тому +4

      Hi Dr Prasad. A cell proliferation assay measures any increase in the number of cells. A cytotoxicity assay measures the capacity of cytotoxic compounds to cause cell death, so markers of cell death are measured.

    • @kosheeka
      @kosheeka 5 місяців тому

      Cell proliferation assays measure cell growth, while cytotoxicity assays assess cell death caused by drugs or other factors. They provide insights into cell health and function, but target different aspects: growth vs. survival.