I know Im asking the wrong place but does anybody know of a trick to get back into an Instagram account?? I was stupid forgot my login password. I would appreciate any assistance you can give me
@Zachary Zain Thanks for your reply. I got to the site through google and I'm in the hacking process atm. Looks like it's gonna take a while so I will reply here later when my account password hopefully is recovered.
So in my opinion, if you substract the mean absorbance of the negative control from the absorbance (exp), you also have to substract it from the poisitive control or it will falsify your results, because if you don't do that, technically a 100% viability isn't even possible... or am I missing something?
Great video! The video explained many detail things and combined examples to better understand the technique. It is hard to find such high-quality video in youtube. Hope to provide more common skills and techniques in research. Thank you!
Overall good presentation, however the formula should be corrected: the absorbance value of the blank wells (negative control) must be subtracted from all samples, including from the positive control samples. That's why your 100 % viability is not 100%.
We typically make our graphs and calculations using a combination of excel and prism. You can see more details about that in this video - ua-cam.com/video/TnSAdtrwGI8/v-deo.html
A very helpful video. The viability of positive control wells doesn't average to 100% in this presentation. I think it's because you calculated the average of raw positive control data before subtracting the average of the blank from the value of each well.
I'm going to perform MTT assay using cancer cell Line, I want to know how can I decide concentration for any extracts or phytoconstituent to gate good results.. please reply me waiting for your answer Mam 😊🙏
The final concentration of MTT that you’ve used is 5ug/ml. Bt i guess it’s actually 0.2mg/ml - 0.5mg/ml. In our lab we’re routinely performing the assay and we use 0.5mg/ml concentration.
The step 2 Add MTT is not clear.. Can anyone please explain 1. Make 5mg/ml stock in PBS 2. From this dilute how much amount ? After this process is not visible
why we have to do the dose or concentration of the drug as log scale? would you give me more explanation about this please. Thank you so much. Such a good video
This is the one I’ve been looking for after many years. Has anyone heard of a stereotypical laboratory that involves this same form of laboratory equipment? That’s why I came here.
This is the first of its kind video with such an excellent expanation to MTT on youtube. However, I have a question related to the negative control. Does negative control include only DMSO or it should be MTT + DMSO?
Thank you so much for the positive comment. The negative control should have no cells or drug treatments. You can do it two ways - you can either just add DMSO to it at the end and subtract the reading out as background. Or if you want to be more rigorous, you can add plain media to it (no cells or treatments), then add the MTT reagent mix, and then add just DMSO. The results should be similar since there are no cells so there will be no reduction or crystal formation. If you want to see more detailed versions of these videos, you can also check out additional videos (many more coming soon) here: ua-cam.com/channels/zMh8ngHIzPB-bJCveXrhjg.html
We usually do 6-8 replicates, just because MTTs can sometimes have higher error and more replicates helps manage the error bars, but 3 will also be ok if you get very consistent results.
Your explanation is exceptionally clear and very detailed!
What a great channel you have! Everything a scientist need to know is available here. Thanks a lot & keep going.
I love love love how detailed and direct the entire video is! Thank you so much!
I know Im asking the wrong place but does anybody know of a trick to get back into an Instagram account??
I was stupid forgot my login password. I would appreciate any assistance you can give me
@Idris Langston instablaster :)
@Zachary Zain Thanks for your reply. I got to the site through google and I'm in the hacking process atm.
Looks like it's gonna take a while so I will reply here later when my account password hopefully is recovered.
@Zachary Zain It did the trick and I actually got access to my account again. I am so happy!
Thanks so much, you really help me out!
@Idris Langston No problem xD
A very tactful way to understand the metabolic activity of certain cell cultures. Good work.
It has been a long time since I've seen such a well explained video. Truly exceptional!!
I have been doing MTT from last few years now and i can tell u that it is one of the best video explanation of the assay. Nice job👍
Aoa bro. Can I have your contact email?
So in my opinion, if you substract the mean absorbance of the negative control from the absorbance (exp), you also have to substract it from the poisitive control or it will falsify your results, because if you don't do that, technically a 100% viability isn't even possible... or am I missing something?
Great video! The video explained many detail things and combined examples to better understand the technique. It is hard to find such high-quality video in youtube. Hope to provide more common skills and techniques in research. Thank you!
WE ARE EXCITED TO SEE THAT THIS VIDEO PROTOCOL WAS USEFUL TO YOU.
Why is the blank value not also subtracted from the mean value of the positive control? Doesn't it also have the same DMSO background noise?
That was a splendid discussion. However, i was wondering if you do really enjoy your work, because your voice says otherwise.
A fantasic video and explanation except that sickening vocal fry
Overall good presentation, however the formula should be corrected: the absorbance value of the blank wells (negative control) must be subtracted from all samples, including from the positive control samples. That's why your 100 % viability is not 100%.
It was really helpful and I really wanna say thank you to you all.
Thank you very much for the explanation. With which program did you do the graphs and IC50 calculation?
We typically make our graphs and calculations using a combination of excel and prism. You can see more details about that in this video - ua-cam.com/video/TnSAdtrwGI8/v-deo.html
Gracias
This was the best MTT tutorial I have watched . Could you please do a video for ELISA and RT PCR?
Thank you
There is one for qPCR here: ua-cam.com/video/EuXUpI0b-q4/v-deo.html - Glad they are helpful to you!
A very helpful video. The viability of positive control wells doesn't average to 100% in this presentation. I think it's because you calculated the average of raw positive control data before subtracting the average of the blank from the value of each well.
Yes and because of that the relative viability of the samples are not % of the positive control as it was intended.
I'm going to perform MTT assay using cancer cell Line, I want to know how can I decide concentration for any extracts or phytoconstituent to gate good results.. please reply me waiting for your answer Mam 😊🙏
so positive control= cell+ mtt agent+ dmso and negative control = mtt+ media+ dmso
only?
This video just explains everything I have being looking for in MTT assay. Thank you so much
thanks for the very informative video..can i use an expired dmso for this assay?
You have taken your time to give a detailed explanation. good job and kudos to you
So helpful! Thank you!
hi could you please provide reference or article showing the % viability equation?
The final concentration of MTT that you’ve used is 5ug/ml. Bt i guess it’s actually 0.2mg/ml - 0.5mg/ml. In our lab we’re routinely performing the assay and we use 0.5mg/ml concentration.
The step 2 Add MTT is not clear.. Can anyone please explain
1. Make 5mg/ml stock in PBS
2. From this dilute how much amount ? After this process is not visible
Why you stopped making Videos?
Your Voice is so good and easy to follow🙏🙏
Start uploading if possible... I hope everything is fine
Can water be used as solvent for such cell culturing and performing various viability assays just as DMSO?
If not then y?
This was a very helpful video! Where would you recommend purchasing the MTT assay, and would it include this protocol?
why we have to do the dose or concentration of the drug as log scale? would you give me more explanation about this please. Thank you so much. Such a good video
so helpful Video, thank a million
really nice
I loved your approach
This is the one I’ve been looking for after many years.
Has anyone heard of a stereotypical laboratory that involves this same form of laboratory equipment? That’s why I came here.
THE BEST! THANK YOU!
You sound just like my friend Amy's soothing voice, which states for: piece of cake.
It is one of the best vedios for explanation Mtt assay i have seen really perfect 👏👏👏
Can MTT be used for virus quantification?
No. However, it can indirectly measure toxicity induced by virus infection.
Thank you so much!
Very informative and helpful video to know all about MTT assay. Thank u..
Thank you🌹
Very helpful. Thank you!
big help, thank youu!!
This was highly informative and useful for what I'm doing. Thanks a lot.
Thanks alot
Very good presentation, helps me a lot! Thanks!!!
Such an amazing video!
This is the first of its kind video with such an excellent expanation to MTT on youtube. However, I have a question related to the negative control.
Does negative control include only DMSO or it should be MTT + DMSO?
Thank you so much for the positive comment. The negative control should have no cells or drug treatments. You can do it two ways - you can either just add DMSO to it at the end and subtract the reading out as background. Or if you want to be more rigorous, you can add plain media to it (no cells or treatments), then add the MTT reagent mix, and then add just DMSO. The results should be similar since there are no cells so there will be no reduction or crystal formation. If you want to see more detailed versions of these videos, you can also check out additional videos (many more coming soon) here: ua-cam.com/channels/zMh8ngHIzPB-bJCveXrhjg.html
Much love & appreciation
❤❤❤ exceptional
So helpful for me. Thank you so much. No one can elaborate so nicely like did.
Does this specific protocol have to be in 6 replicates, or okay to seed in triplicate?
We usually do 6-8 replicates, just because MTTs can sometimes have higher error and more replicates helps manage the error bars, but 3 will also be ok if you get very consistent results.
Really impressive and informative content 👏
Thank you!🤩
Nice
how do u calculate p value for this one?
www.socscistatistics.com/pvalues/tdistribution.aspx
ua-cam.com/video/HoUjqVJdFhQ/v-deo.html
Very confusing 😢